4cts - Revision history

OCA at 10:42, 1 March 2024

OCA — Fri, 01 Mar 2024 10:42:12 GMT

←Older revision		Revision as of 10:42, 1 March 2024
Line 3:		Line 3:
	<StructureSection load='4cts' size='340' side='right'caption='[[4cts]], [[Resolution\|resolution]] 2.90Å' scene=''>		<StructureSection load='4cts' size='340' side='right'caption='[[4cts]], [[Resolution\|resolution]] 2.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4cts]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Pig Pig~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CTS OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4CTS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4cts]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CTS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4CTS FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name~~=~~'pdbligand=OAA:OXALOACETATE+ION'>OAA</scene~~></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.9Å</td></tr>
-	<tr id='~~activity~~'><td class="sblockLbl"><b>~~Activity~~:</b></td><td class="sblockDat"><~~span class~~='~~plainlinks~~'>~~[http://en.wikipedia.org/wiki/Citrate_(Si)-synthase Citrate (Si)-synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.3.1 2.3.3.1]~~ </~~span~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=OAA:OXALOACETATE+ION'>OAA</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4cts FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cts OCA], [~~http~~://pdbe.org/4cts PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4cts RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4cts PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4cts ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4cts FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cts OCA], [https://pdbe.org/4cts PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4cts RCSB], [https://www.ebi.ac.uk/pdbsum/4cts PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4cts ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/CISY_PIG CISY_PIG]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 18:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=4cts ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=4cts ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The crystal structure of the complex of pig heart citrate synthase and oxaloacetate in the presence of the potent inhibitor S-acetonyl coenzyme A has been determined at a nominal resolution of 2.9 A by Patterson search techniques and refined by restrained crystallographic refinement. The complex crystallizes in the presence of polyvinylpyrrolidone in space group P4(3)2(1)2 with a = 101.5 A and c = 224.6 A, with one dimeric molecule of molecular weight 100,000 in the asymmetric unit. The crystallographic R factor is 0.194 for the 14,332 unique reflections between 6.0 and 2.9 A resolution. The structures of two forms of citrate synthase in the presence and absence of product molecules have been determined recently and shown to differ in the relative arrangement of the large and small domains ("closed" and "open" forms). The third crystal form described here is also closed, but there is substantial rearrangement within the small domain relative to either of the other crystal forms. We conclude that this is a third structural state of the enzyme, and catalytic activity of the enzyme depends on structural changes during the course of the reaction affecting domain conformation also. The three structures are compared, and it is shown that the large domain is considerably more rigid than the small domain. The conformation of the small domain adapts to the ligand. The inhibitor, and the "coenzyme-A-binding segment" of the enzyme are disordered. No electron density is observed for the inhibitor, and only weak density for the coenzyme-A-binding segment. Electron density for oxaloacetate is well defined. It binds in a very similar manner to citrate.
-
-	Crystal structure analysis and molecular model of a complex of citrate synthase with oxaloacetate and S-acetonyl-coenzyme A.,Wiegand G, Remington S, Deisenhofer J, Huber R J Mol Biol. 1984 Mar 25;174(1):205-19. PMID:6716477<ref>PMID:6716477</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 4cts" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Citrate Synthase 3D structures\|Citrate Synthase 3D structures]]		*[[Citrate Synthase 3D structures\|Citrate Synthase 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Pig~~]]	+	[[Category: Sus scrofa]]
-	[[Category: Huber, R]]	+	[[Category: Huber R]]
-	[[Category: Remington, S]]	+	[[Category: Remington S]]
-	[[Category: Wiegand, G]]	+	[[Category: Wiegand G]]
-	~~[[Category: Oxo-acid-lyase~~]]	+

OCA at 09:30, 5 February 2020

OCA — Wed, 05 Feb 2020 09:30:52 GMT

←Older revision		Revision as of 09:30, 5 February 2020
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	==CRYSTAL STRUCTURE ANALYSIS AND MOLECULAR MODEL OF A COMPLEX OF CITRATE SYNTHASE WITH OXALOACETATE AND S-ACETONYL-COENZYME A==		==CRYSTAL STRUCTURE ANALYSIS AND MOLECULAR MODEL OF A COMPLEX OF CITRATE SYNTHASE WITH OXALOACETATE AND S-ACETONYL-COENZYME A==
-	<StructureSection load='4cts' size='340' side='right' caption='[[4cts]], [[Resolution\|resolution]] 2.90Å' scene=''>	+	<StructureSection load='4cts' size='340' side='right'caption='[[4cts]], [[Resolution\|resolution]] 2.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4cts]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pig Pig]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CTS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4CTS FirstGlance]. <br>		<table><tr><td colspan='2'>[[4cts]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pig Pig]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CTS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4CTS FirstGlance]. <br>
Line 12:		Line 12:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ct/4cts_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ct/4cts_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 27:		Line 27:
	</div>		</div>
	<div class="pdbe-citations 4cts" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4cts" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Citrate Synthase 3D structures\|Citrate Synthase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Pig]]		[[Category: Pig]]
	[[Category: Huber, R]]		[[Category: Huber, R]]

OCA at 07:19, 29 November 2017

OCA — Wed, 29 Nov 2017 07:19:05 GMT

←Older revision		Revision as of 07:19, 29 November 2017
Line 1:		Line 1:
		+
	==CRYSTAL STRUCTURE ANALYSIS AND MOLECULAR MODEL OF A COMPLEX OF CITRATE SYNTHASE WITH OXALOACETATE AND S-ACETONYL-COENZYME A==		==CRYSTAL STRUCTURE ANALYSIS AND MOLECULAR MODEL OF A COMPLEX OF CITRATE SYNTHASE WITH OXALOACETATE AND S-ACETONYL-COENZYME A==
	<StructureSection load='4cts' size='340' side='right' caption='[[4cts]], [[Resolution\|resolution]] 2.90Å' scene=''>		<StructureSection load='4cts' size='340' side='right' caption='[[4cts]], [[Resolution\|resolution]] 2.90Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=OAA:OXALOACETATE+ION'>OAA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=OAA:OXALOACETATE+ION'>OAA</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Citrate_(Si)-synthase Citrate (Si)-synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.3.1 2.3.3.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Citrate_(Si)-synthase Citrate (Si)-synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.3.1 2.3.3.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4cts FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cts OCA], [http://pdbe.org/4cts PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4cts RCSB], [http://www.ebi.ac.uk/pdbsum/4cts PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4cts FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cts OCA], [http://pdbe.org/4cts PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4cts RCSB], [http://www.ebi.ac.uk/pdbsum/4cts PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4cts ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 26:		Line 27:
	</div>		</div>
	<div class="pdbe-citations 4cts" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4cts" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Citrate Synthase\|Citrate Synthase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 04:30, 10 February 2016

OCA — Wed, 10 Feb 2016 04:30:18 GMT

←Older revision		Revision as of 04:30, 10 February 2016
Line 2:		Line 2:
	<StructureSection load='4cts' size='340' side='right' caption='[[4cts]], [[Resolution\|resolution]] 2.90Å' scene=''>		<StructureSection load='4cts' size='340' side='right' caption='[[4cts]], [[Resolution\|resolution]] 2.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4cts]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Sus_scrofa Sus scrofa~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CTS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4CTS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4cts]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pig Pig]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CTS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4CTS FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=OAA:OXALOACETATE+ION'>OAA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=OAA:OXALOACETATE+ION'>OAA</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Citrate_(Si)-synthase Citrate (Si)-synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.3.1 2.3.3.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Citrate_(Si)-synthase Citrate (Si)-synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.3.1 2.3.3.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4cts FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cts OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4cts RCSB], [http://www.ebi.ac.uk/pdbsum/4cts PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4cts FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cts OCA], [http://pdbe.org/4cts PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4cts RCSB], [http://www.ebi.ac.uk/pdbsum/4cts PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 15:		Line 15:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=4cts ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 25:		Line 25:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4cts" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 32:		Line 33:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Sus scrofa~~]]	+	[[Category: Pig]]
	[[Category: Huber, R]]		[[Category: Huber, R]]
	[[Category: Remington, S]]		[[Category: Remington, S]]
	[[Category: Wiegand, G]]		[[Category: Wiegand, G]]
	[[Category: Oxo-acid-lyase]]		[[Category: Oxo-acid-lyase]]

OCA at 10:40, 20 November 2014

OCA — Thu, 20 Nov 2014 10:40:57 GMT

←Older revision		Revision as of 10:40, 20 November 2014
Line 1:		Line 1:
-	[[~~Image~~:4cts.~~png\|left~~\|200px]]	+	==CRYSTAL STRUCTURE ANALYSIS AND MOLECULAR MODEL OF A COMPLEX OF CITRATE SYNTHASE WITH OXALOACETATE AND S-ACETONYL-COENZYME A==
		+	<StructureSection load='4cts' size='340' side='right' caption='[[4cts]], [[Resolution\|resolution]] 2.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4cts]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CTS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4CTS FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=OAA:OXALOACETATE+ION'>OAA</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Citrate_(Si)-synthase Citrate (Si)-synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.3.1 2.3.3.1] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4cts FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cts OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4cts RCSB], [http://www.ebi.ac.uk/pdbsum/4cts PDBsum]</span></td></tr>
		+	</table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ct/4cts_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The crystal structure of the complex of pig heart citrate synthase and oxaloacetate in the presence of the potent inhibitor S-acetonyl coenzyme A has been determined at a nominal resolution of 2.9 A by Patterson search techniques and refined by restrained crystallographic refinement. The complex crystallizes in the presence of polyvinylpyrrolidone in space group P4(3)2(1)2 with a = 101.5 A and c = 224.6 A, with one dimeric molecule of molecular weight 100,000 in the asymmetric unit. The crystallographic R factor is 0.194 for the 14,332 unique reflections between 6.0 and 2.9 A resolution. The structures of two forms of citrate synthase in the presence and absence of product molecules have been determined recently and shown to differ in the relative arrangement of the large and small domains ("closed" and "open" forms). The third crystal form described here is also closed, but there is substantial rearrangement within the small domain relative to either of the other crystal forms. We conclude that this is a third structural state of the enzyme, and catalytic activity of the enzyme depends on structural changes during the course of the reaction affecting domain conformation also. The three structures are compared, and it is shown that the large domain is considerably more rigid than the small domain. The conformation of the small domain adapts to the ligand. The inhibitor, and the "coenzyme-A-binding segment" of the enzyme are disordered. No electron density is observed for the inhibitor, and only weak density for the coenzyme-A-binding segment. Electron density for oxaloacetate is well defined. It binds in a very similar manner to citrate.

-	~~{{STRUCTURE_4cts\| PDB=4cts \| SCENE= }}~~	+	Crystal structure analysis and molecular model of a complex of citrate synthase with oxaloacetate and S-acetonyl-coenzyme A.,Wiegand G, Remington S, Deisenhofer J, Huber R J Mol Biol. 1984 Mar 25;174(1):205-19. PMID:6716477<ref>PMID:6716477</ref>

-	~~===CRYSTAL STRUCTURE ANALYSIS AND MOLECULAR MODEL OF A COMPLEX OF CITRATE SYNTHASE WITH OXALOACETATE AND S-ACETONYL-COENZYME A===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_6716477}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[4cts]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Sus_scrofa Sus scrofa]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=4CTS OCA].~~	+

	==See Also==		==See Also==
	*[[Citrate Synthase\|Citrate Synthase]]		*[[Citrate Synthase\|Citrate Synthase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:006716477<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Sus scrofa]]		[[Category: Sus scrofa]]
-	[[Category: Huber, R.]]	+	[[Category: Huber, R]]
-	[[Category: Remington, S.]]	+	[[Category: Remington, S]]
-	[[Category: Wiegand, G.]]	+	[[Category: Wiegand, G]]
	[[Category: Oxo-acid-lyase]]		[[Category: Oxo-acid-lyase]]

OCA at 16:20, 20 October 2012

OCA — Sat, 20 Oct 2012 16:20:24 GMT

←Older revision		Revision as of 16:20, 20 October 2012
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	==About this Structure==		==About this Structure==
-	[[4cts]] is a 2 chain structure ~~of [[Citrate Synthase]]~~ with sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CTS OCA].	+	[[4cts]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CTS OCA].

	==See Also==		==See Also==

OCA: Protected "4cts" [edit=sysop:move=sysop]

OCA — Thu, 26 Jul 2012 21:21:40 GMT

Protected "4cts" [edit=sysop:move=sysop]

←Older revision

Revision as of 21:21, 26 July 2012

OCA at 21:21, 26 July 2012

OCA — Thu, 26 Jul 2012 21:21:38 GMT

←Older revision		Revision as of 21:21, 26 July 2012
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-	{{Seed}}
	[[Image:4cts.png\|left\|200px]]		[[Image:4cts.png\|left\|200px]]

-	<!--
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	{{STRUCTURE_4cts\| PDB=4cts \| SCENE= }}		{{STRUCTURE_4cts\| PDB=4cts \| SCENE= }}

	===CRYSTAL STRUCTURE ANALYSIS AND MOLECULAR MODEL OF A COMPLEX OF CITRATE SYNTHASE WITH OXALOACETATE AND S-ACETONYL-COENZYME A===		===CRYSTAL STRUCTURE ANALYSIS AND MOLECULAR MODEL OF A COMPLEX OF CITRATE SYNTHASE WITH OXALOACETATE AND S-ACETONYL-COENZYME A===

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	{{ABSTRACT_PUBMED_6716477}}		{{ABSTRACT_PUBMED_6716477}}

	==About this Structure==		==About this Structure==
-	~~4CTS~~ is a 2 ~~chains~~ structure of ~~sequences~~ from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CTS OCA].	+	[[4cts]] is a 2 chain structure of [[Citrate Synthase]] with sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CTS OCA].
		+
		+	==See Also==
		+	*[[Citrate Synthase\|Citrate Synthase]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~6716477~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:006716477</ref><references group="xtra"/>
	[[Category: Sus scrofa]]		[[Category: Sus scrofa]]
	[[Category: Huber, R.]]		[[Category: Huber, R.]]
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	[[Category: Wiegand, G.]]		[[Category: Wiegand, G.]]
	[[Category: Oxo-acid-lyase]]		[[Category: Oxo-acid-lyase]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 15:26:43 2009''

OCA at 13:26, 17 February 2009

OCA — Tue, 17 Feb 2009 13:26:43 GMT

←Older revision		Revision as of 13:26, 17 February 2009
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	==About this Structure==		==About this Structure==
-	4CTS is a ~~[[Single protein]]~~ structure of ~~sequence~~ from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CTS OCA].	+	4CTS is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CTS OCA].

	==Reference==		==Reference==
-	Crystal structure analysis and molecular model of a complex of citrate synthase with oxaloacetate and S-acetonyl-coenzyme A., Wiegand G, Remington S, Deisenhofer J, Huber R, J Mol Biol. 1984 Mar 25;174(1):205-19. PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/6716477 6716477]~~	+	<ref group="xtra">PMID:6716477</ref><references group="xtra"/>
-	~~[[Category: Single protein]]~~	+
	[[Category: Sus scrofa]]		[[Category: Sus scrofa]]
	[[Category: Huber, R.]]		[[Category: Huber, R.]]
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	[[Category: Oxo-acid-lyase]]		[[Category: Oxo-acid-lyase]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Thu Jul 3 13~~:33:~~57 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 15:26:43 2009''

OCA at 10:33, 3 July 2008

OCA — Thu, 03 Jul 2008 10:33:58 GMT

←Older revision		Revision as of 10:33, 3 July 2008
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-	[[Image:4cts.~~gif~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:4cts.png\|left\|200px]]

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	{{STRUCTURE_4cts\| PDB=4cts \| SCENE= }}		{{STRUCTURE_4cts\| PDB=4cts \| SCENE= }}

-	~~'''~~CRYSTAL STRUCTURE ANALYSIS AND MOLECULAR MODEL OF A COMPLEX OF CITRATE SYNTHASE WITH OXALOACETATE AND S-ACETONYL-COENZYME A~~'''~~	+	===CRYSTAL STRUCTURE ANALYSIS AND MOLECULAR MODEL OF A COMPLEX OF CITRATE SYNTHASE WITH OXALOACETATE AND S-ACETONYL-COENZYME A===


-	~~==Overview==~~	+	<!--
-	The crystal structure of the complex of pig heart citrate synthase and oxaloacetate in the presence of the potent inhibitor S-acetonyl coenzyme A has been determined at a nominal resolution of 2.9 A by Patterson search techniques and refined by restrained crystallographic refinement. The complex crystallizes in the presence of polyvinylpyrrolidone in space group P4(3)2(1)2 with a = 101.5 A and c = 224.6 A, ~~with one dimeric molecule of molecular weight 100~~,~~000 in~~ the asymmetric unit. The crystallographic R factor is 0.194 for the 14,332 unique reflections between 6.0 and 2.9 A resolution. The structures of two forms of citrate synthase in the presence and absence of product molecules have been determined recently and shown to ~~differ in~~ the ~~relative arrangement of the large and small domains~~ (~~"closed" and "open" forms)~~. ~~The third crystal form described here is also closed, but there is substantial rearrangement within the small domain relative to either of the other crystal forms~~. ~~We conclude that this is a third structural state of the enzyme~~, ~~and catalytic activity of the enzyme depends on structural changes during the course of the reaction affecting domain conformation also. The three structures are compared, and it~~ is ~~shown that~~ the ~~large domain is considerably more rigid than the small domain~~. ~~The conformation of the small domain adapts to the ligand. The inhibitor, and the "coenzyme~~-A-binding segment" of the enzyme are disordered. No electron density is observed for the inhibitor, and only weak density for the coenzyme-A-binding segment. Electron density for oxaloacetate is well defined. It binds in a very similar manner to citrate.	+	The line below this paragraph, {{ABSTRACT_PUBMED_6716477}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 6716477 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_6716477}}

	==About this Structure==		==About this Structure==
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	[[Category: Wiegand, G.]]		[[Category: Wiegand, G.]]
	[[Category: Oxo-acid-lyase]]		[[Category: Oxo-acid-lyase]]
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sun May~~ ~~4 22~~:22:49 2008''	+
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