4cwe - Revision history

OCA at 12:17, 20 December 2023

OCA — Wed, 20 Dec 2023 12:17:16 GMT

←Older revision		Revision as of 12:17, 20 December 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4cwe]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CWE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4CWE FirstGlance]. <br>		<table><tr><td colspan='2'>[[4cwe]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CWE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4CWE FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4cwe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cwe OCA], [https://pdbe.org/4cwe PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4cwe RCSB], [https://www.ebi.ac.uk/pdbsum/4cwe PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4cwe ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4cwe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cwe OCA], [https://pdbe.org/4cwe PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4cwe RCSB], [https://www.ebi.ac.uk/pdbsum/4cwe PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4cwe ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/REPN_STAAU REPN_STAAU]] This protein is probably a specific topoisomerase involved in initiating replication. This protein is specifically required and may be rate-limiting for replication of the plasmid in vivo.[[https://www.uniprot.org/uniprot/REPD_STAAU REPD_STAAU]] This protein is probably a specific topoisomerase involved in initiating replication. This protein is specifically required and may be rate-limiting for replication of the plasmid in vivo.	+	[https://www.uniprot.org/uniprot/REPN_STAAU REPN_STAAU] This protein is probably a specific topoisomerase involved in initiating replication. This protein is specifically required and may be rate-limiting for replication of the plasmid in vivo.[https://www.uniprot.org/uniprot/REPD_STAAU REPD_STAAU] This protein is probably a specific topoisomerase involved in initiating replication. This protein is specifically required and may be rate-limiting for replication of the plasmid in vivo.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 07:35, 14 September 2022

OCA — Wed, 14 Sep 2022 07:35:59 GMT

←Older revision		Revision as of 07:35, 14 September 2022
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	<StructureSection load='4cwe' size='340' side='right'caption='[[4cwe]], [[Resolution\|resolution]] 3.00Å' scene=''>		<StructureSection load='4cwe' size='340' side='right'caption='[[4cwe]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4cwe]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus~~ aureus~~" (rosenbach 1884) zopf 1885~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CWE OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4CWE FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4cwe]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CWE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4CWE FirstGlance]. <br>
-	</td></tr>~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4cwc\|4cwc]]</td></tr>~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4cwe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cwe OCA], [https://pdbe.org/4cwe PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4cwe RCSB], [https://www.ebi.ac.uk/pdbsum/4cwe PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4cwe ProSAT]</span></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA_topoisomerase DNA topoisomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.99.1.2 5.99.1.2] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4cwe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cwe OCA], [~~http~~://pdbe.org/4cwe PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4cwe RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4cwe PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4cwe ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/REPD_STAAU REPD_STAAU]] This protein is probably a specific topoisomerase involved in initiating replication. This protein is specifically required and may be rate-limiting for replication of the plasmid in vivo.	+	[[https://www.uniprot.org/uniprot/REPN_STAAU REPN_STAAU]] This protein is probably a specific topoisomerase involved in initiating replication. This protein is specifically required and may be rate-limiting for replication of the plasmid in vivo.[[https://www.uniprot.org/uniprot/REPD_STAAU REPD_STAAU]] This protein is probably a specific topoisomerase involved in initiating replication. This protein is specifically required and may be rate-limiting for replication of the plasmid in vivo.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: DNA topoisomerase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Carr, S B~~]]	+	[[Category: Staphylococcus aureus]]
-	[[Category: ~~Phillips, S E.V~~]]	+	[[Category: Carr SB]]
-	[[Category: ~~Thomas, C D~~]]	+	[[Category: Phillips SEV]]
-	[[Category: ~~Antibiotic resistance]]~~	+	[[Category: Thomas CD]]
-	~~[[Category: Chimera]]~~	+
-	~~[[Category: Dna relaxase]]~~	+
-	~~[[Category: Isomerase]]~~	+
-	~~[[Category: Pcra helicase~~]]	+

OCA at 09:57, 20 March 2019

OCA — Wed, 20 Mar 2019 09:57:09 GMT

←Older revision		Revision as of 09:57, 20 March 2019
Line 1:		Line 1:

	==Structural studies of rolling circle replication initiation protein from Staphylococcus aureus==		==Structural studies of rolling circle replication initiation protein from Staphylococcus aureus==
-	<StructureSection load='4cwe' size='340' side='right' caption='[[4cwe]], [[Resolution\|resolution]] 3.00Å' scene=''>	+	<StructureSection load='4cwe' size='340' side='right'caption='[[4cwe]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4cwe]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CWE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4CWE FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4cwe]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CWE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4CWE FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4cwc\|4cwc]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4cwc\|4cwc]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA_topoisomerase DNA topoisomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.99.1.2 5.99.1.2] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA_topoisomerase DNA topoisomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.99.1.2 5.99.1.2] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4cwe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cwe OCA], [http://pdbe.org/4cwe PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4cwe RCSB], [http://www.ebi.ac.uk/pdbsum/4cwe PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4cwe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cwe OCA], [http://pdbe.org/4cwe PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4cwe RCSB], [http://www.ebi.ac.uk/pdbsum/4cwe PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4cwe ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 24:		Line 24:
	</StructureSection>		</StructureSection>
	[[Category: DNA topoisomerase]]		[[Category: DNA topoisomerase]]
		+	[[Category: Large Structures]]
	[[Category: Carr, S B]]		[[Category: Carr, S B]]
	[[Category: Phillips, S E.V]]		[[Category: Phillips, S E.V]]

OCA at 17:32, 10 May 2016

OCA — Tue, 10 May 2016 17:32:03 GMT

←Older revision		Revision as of 17:32, 10 May 2016
Line 1:		Line 1:
		+
	==Structural studies of rolling circle replication initiation protein from Staphylococcus aureus==		==Structural studies of rolling circle replication initiation protein from Staphylococcus aureus==
	<StructureSection load='4cwe' size='340' side='right' caption='[[4cwe]], [[Resolution\|resolution]] 3.00Å' scene=''>		<StructureSection load='4cwe' size='340' side='right' caption='[[4cwe]], [[Resolution\|resolution]] 3.00Å' scene=''>

OCA at 07:53, 3 February 2016

OCA — Wed, 03 Feb 2016 07:53:40 GMT

←Older revision		Revision as of 07:53, 3 February 2016
Line 5:		Line 5:
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4cwc\|4cwc]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4cwc\|4cwc]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA_topoisomerase DNA topoisomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.99.1.2 5.99.1.2] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA_topoisomerase DNA topoisomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.99.1.2 5.99.1.2] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4cwe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cwe OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4cwe RCSB], [http://www.ebi.ac.uk/pdbsum/4cwe PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4cwe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cwe OCA], [http://pdbe.org/4cwe PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4cwe RCSB], [http://www.ebi.ac.uk/pdbsum/4cwe PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/REPD_STAAU REPD_STAAU]] This protein is probably a specific topoisomerase involved in initiating replication. This protein is specifically required and may be rate-limiting for replication of the plasmid in vivo.		[[http://www.uniprot.org/uniprot/REPD_STAAU REPD_STAAU]] This protein is probably a specific topoisomerase involved in initiating replication. This protein is specifically required and may be rate-limiting for replication of the plasmid in vivo.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Antibiotic resistance in pathogenic bacteria is a continual threat to human health, often residing in extrachromosomal plasmid DNA. Plasmids of the pT181 family are widespread and confer various antibiotic resistances to Staphylococcus aureus. They replicate via a rolling circle mechanism that requires a multi-functional, plasmid-encoded replication protein to initiate replication, recruit a helicase to the site of initiation and terminate replication after DNA synthesis is complete. We present the first atomic resolution structures of three such replication proteins that reveal distinct, functionally relevant conformations. The proteins possess a unique active site and have been shown to contain a catalytically essential metal ion that is bound in a manner distinct from that of any other rolling circle replication proteins. These structures are the first examples of the Rep_trans Pfam family providing insights into the replication of numerous antibiotic resistance plasmids from Gram-positive bacteria, Gram-negative phage and the mobilisation of DNA by conjugative transposons.
		+
		+	Structures of replication initiation proteins from staphylococcal antibiotic resistance plasmids reveal protein asymmetry and flexibility are necessary for replication.,Carr SB, Phillips SE, Thomas CD Nucleic Acids Res. 2016 Jan 20. pii: gkv1539. PMID:26792891<ref>PMID:26792891</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 4cwe" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 12:56, 15 April 2015

OCA — Wed, 15 Apr 2015 12:56:56 GMT

←Older revision		Revision as of 12:56, 15 April 2015
Line 1:		Line 1:
-	'''~~Unreleased structure~~'''	+	==Structural studies of rolling circle replication initiation protein from Staphylococcus aureus==
-		+	<StructureSection load='4cwe' size='340' side='right' caption='[[4cwe]], [[Resolution\|resolution]] 3.00Å' scene=''>
-	~~The entry~~ 4cwe is ~~ON HOLD until Paper Publication~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[4cwe]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CWE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4CWE FirstGlance]. <br>
-	~~Authors~~: ~~Carr~~, S.B., ~~Phillips~~, S.E.V., ~~Thomas, C~~.D.	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4cwc\|4cwc]]</td></tr>
-		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA_topoisomerase DNA topoisomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.99.1.2 5.99.1.2] </span></td></tr>
-	~~Description: Structural studies of rolling circle replication initiation protein from Staphylococcus aureus~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4cwe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cwe OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4cwe RCSB], [http://www.ebi.ac.uk/pdbsum/4cwe PDBsum]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[Category: ~~Thomas, C.D~~]]	+	== Function ==
-	[[Category: Carr, S.B]]	+	[[http://www.uniprot.org/uniprot/REPD_STAAU REPD_STAAU]] This protein is probably a specific topoisomerase involved in initiating replication. This protein is specifically required and may be rate-limiting for replication of the plasmid in vivo.
-	[[Category: Phillips, S.E.V]]	+	__TOC__
		+	</StructureSection>
		+	[[Category: DNA topoisomerase]]
		+	[[Category: Carr, S B]]
		+	[[Category: Phillips, S E.V]]
		+	[[Category: Thomas, C D]]
		+	[[Category: Antibiotic resistance]]
		+	[[Category: Chimera]]
		+	[[Category: Dna relaxase]]
		+	[[Category: Isomerase]]
		+	[[Category: Pcra helicase]]

OCA at 13:36, 24 December 2014

OCA — Wed, 24 Dec 2014 13:36:40 GMT

←Older revision		Revision as of 13:36, 24 December 2014
Line 6:		Line 6:

	Description: Structural studies of rolling circle replication initiation protein from Staphylococcus aureus		Description: Structural studies of rolling circle replication initiation protein from Staphylococcus aureus
		+	[[Category: Unreleased Structures]]
		+	[[Category: Thomas, C.D]]
		+	[[Category: Carr, S.B]]
		+	[[Category: Phillips, S.E.V]]

OCA at 07:22, 7 May 2014

OCA — Wed, 07 May 2014 07:22:02 GMT

←Older revision		Revision as of 07:22, 7 May 2014
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4cwe is ON HOLD until ~~sometime in the future~~	+	The entry 4cwe is ON HOLD until Paper Publication

	Authors: Carr, S.B., Phillips, S.E.V., Thomas, C.D.		Authors: Carr, S.B., Phillips, S.E.V., Thomas, C.D.

	Description: Structural studies of rolling circle replication initiation protein from Staphylococcus aureus		Description: Structural studies of rolling circle replication initiation protein from Staphylococcus aureus

OCA: Protected "4cwe" [edit=sysop:move=sysop]

OCA — Wed, 09 Apr 2014 05:37:21 GMT

Protected "4cwe" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:37, 9 April 2014

OCA: New page: '''Unreleased structure''' The entry 4cwe is ON HOLD until sometime in the future Authors: Carr, S.B., Phillips, S.E.V., Thomas, C.D. Description: Structural studies of rolling circle ...

OCA — Wed, 09 Apr 2014 05:37:20 GMT

New page: '''Unreleased structure''' The entry 4cwe is ON HOLD until sometime in the future Authors: Carr, S.B., Phillips, S.E.V., Thomas, C.D. Description: Structural studies of rolling circle ...

New page

'''Unreleased structure'''

The entry 4cwe is ON HOLD until sometime in the future

Authors: Carr, S.B., Phillips, S.E.V., Thomas, C.D.

Description: Structural studies of rolling circle replication initiation protein from Staphylococcus aureus