4cxs - Revision history

OCA at 12:17, 20 December 2023

OCA — Wed, 20 Dec 2023 12:17:35 GMT

←Older revision		Revision as of 12:17, 20 December 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4cxs]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CXS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4CXS FirstGlance]. <br>		<table><tr><td colspan='2'>[[4cxs]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CXS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4CXS FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DDZ:3,3-DIHYDROXY+L-ALANINE'>DDZ</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=SV7:PHENYLPHOSPHONIC+ACID'>SV7</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.3Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DDZ:3,3-DIHYDROXY+L-ALANINE'>DDZ</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=SV7:PHENYLPHOSPHONIC+ACID'>SV7</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4cxs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cxs OCA], [https://pdbe.org/4cxs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4cxs RCSB], [https://www.ebi.ac.uk/pdbsum/4cxs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4cxs ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4cxs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cxs OCA], [https://pdbe.org/4cxs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4cxs RCSB], [https://www.ebi.ac.uk/pdbsum/4cxs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4cxs ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/ARS_PSEAE ARS_PSEAE]]	+	[https://www.uniprot.org/uniprot/ARS_PSEAE ARS_PSEAE]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 07:37, 14 September 2022

OCA — Wed, 14 Sep 2022 07:37:57 GMT

←Older revision		Revision as of 07:37, 14 September 2022
Line 3:		Line 3:
	<StructureSection load='4cxs' size='340' side='right'caption='[[4cxs]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='4cxs' size='340' side='right'caption='[[4cxs]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4cxs]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CXS OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4CXS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4cxs]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CXS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4CXS FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=~~SO4~~:~~SULFATE~~+~~ION~~'>~~SO4~~</scene>, <scene name='pdbligand=~~SV7~~:~~PHENYLPHOSPHONIC~~+~~ACID~~'>~~SV7~~</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DDZ:3,3-DIHYDROXY+L-ALANINE'>DDZ</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=SV7:PHENYLPHOSPHONIC+ACID'>SV7</scene></td></tr>
-	~~<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat">~~<scene name='pdbligand=~~DDZ~~:~~3,3-DIHYDROXY~~+~~L-ALANINE~~'>~~DDZ~~</scene~~></td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4cxs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cxs OCA], [https://pdbe.org/4cxs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4cxs RCSB], [https://www.ebi.ac.uk/pdbsum/4cxs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4cxs ProSAT]</span></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4cxk\|4cxk]], [[4cxu\|4cxu]]</td></tr>~~	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Arylsulfatase Arylsulfatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.6.1 3.1.6.1] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4cxs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cxs OCA], [~~http~~://pdbe.org/4cxs PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4cxs RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4cxs PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4cxs ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/ARS_PSEAE ARS_PSEAE]]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 20:

	==See Also==		==See Also==
-	*[[Sulfatase\|Sulfatase]]	+	*[[Sulfatase 3D structures\|Sulfatase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Arylsulfatase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Fischer, G]]	+	[[Category: Pseudomonas aeruginosa]]
-	[[Category: Hollfelder, F]]	+	[[Category: Fischer G]]
-	[[Category: Hyvonen, M]]	+	[[Category: Hollfelder F]]
-	[[Category: Jonas, S]]	+	[[Category: Hyvonen M]]
-	[[Category: Kintses, B]]	+	[[Category: Jonas S]]
-	[[Category: Loo~~, B v~~]]	+	[[Category: Kintses B]]
-	[[Category: Miton~~, C M~~]]	+	[[Category: Loo Bv]]
-	[[Category: Mohammed~~, M F~~]]	+	[[Category: Miton CM]]
-	[[Category: Tokuriki, N]]	+	[[Category: Mohammed MF]]
-	~~[[Category: Catalytic promiscuity]]~~	+	[[Category: Tokuriki N]]
-	~~[[Category: Directed evolution]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Neutral drift]]~~	+
-	~~[[Category: Sulfatase]]~~	+
-	~~[[Category: Superfamily~~]]	+

OCA at 07:29, 24 April 2019

OCA — Wed, 24 Apr 2019 07:29:11 GMT

←Older revision		Revision as of 07:29, 24 April 2019
Line 1:		Line 1:

	==G4 mutant of PAS, arylsulfatase from Pseudomonas aeruginosa, in complex with Phenylphosphonic acid==		==G4 mutant of PAS, arylsulfatase from Pseudomonas aeruginosa, in complex with Phenylphosphonic acid==
-	<StructureSection load='4cxs' size='340' side='right' caption='[[4cxs]], [[Resolution\|resolution]] 2.30Å' scene=''>	+	<StructureSection load='4cxs' size='340' side='right'caption='[[4cxs]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4cxs]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CXS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4CXS FirstGlance]. <br>		<table><tr><td colspan='2'>[[4cxs]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CXS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4CXS FirstGlance]. <br>
Line 27:		Line 27:
	</StructureSection>		</StructureSection>
	[[Category: Arylsulfatase]]		[[Category: Arylsulfatase]]
		+	[[Category: Large Structures]]
	[[Category: Fischer, G]]		[[Category: Fischer, G]]
	[[Category: Hollfelder, F]]		[[Category: Hollfelder, F]]

OCA at 09:12, 30 January 2019

OCA — Wed, 30 Jan 2019 09:12:03 GMT

←Older revision		Revision as of 09:12, 30 January 2019
Line 1:		Line 1:

-	==G4 mutant of PAS, arylsulfatase from Pseudomonas ~~Aeruginosa~~, in complex with Phenylphosphonic acid==	+	==G4 mutant of PAS, arylsulfatase from Pseudomonas aeruginosa, in complex with Phenylphosphonic acid==
	<StructureSection load='4cxs' size='340' side='right' caption='[[4cxs]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='4cxs' size='340' side='right' caption='[[4cxs]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
Line 19:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 4cxs" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4cxs" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Sulfatase\|Sulfatase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 07:44, 29 August 2018

OCA — Wed, 29 Aug 2018 07:44:00 GMT

←Older revision		Revision as of 07:44, 29 August 2018
Line 3:		Line 3:
	<StructureSection load='4cxs' size='340' side='right' caption='[[4cxs]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='4cxs' size='340' side='right' caption='[[4cxs]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4cxs]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CXS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4CXS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4cxs]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CXS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4CXS FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=SV7:PHENYLPHOSPHONIC+ACID'>SV7</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=SV7:PHENYLPHOSPHONIC+ACID'>SV7</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=DDZ:3,3-DIHYDROXY+L-ALANINE'>DDZ</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=DDZ:3,3-DIHYDROXY+L-ALANINE'>DDZ</scene></td></tr>
Line 10:		Line 10:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4cxs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cxs OCA], [http://pdbe.org/4cxs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4cxs RCSB], [http://www.ebi.ac.uk/pdbsum/4cxs PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4cxs ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4cxs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cxs OCA], [http://pdbe.org/4cxs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4cxs RCSB], [http://www.ebi.ac.uk/pdbsum/4cxs PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4cxs ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The recruitment and evolutionary optimization of promiscuous enzymes is key to the rapid adaptation of organisms to changing environments. Our understanding of the precise mechanisms underlying enzyme repurposing is, however, limited: What are the active-site features that enable the molecular recognition of multiple substrates with contrasting catalytic requirements? To gain insights into the molecular determinants of adaptation in promiscuous enzymes, we performed the laboratory evolution of an arylsulfatase to improve its initially weak phenylphosphonate hydrolase activity. The evolutionary trajectory led to a 100,000-fold enhancement of phenylphosphonate hydrolysis, while the native sulfate and promiscuous phosphate mono- and diester hydrolyses were only marginally affected (</=50-fold). Structural, kinetic, and in silico characterizations of the evolutionary intermediates revealed that two key mutations, T50A and M72V, locally reshaped the active site, improving access to the catalytic machinery for the phosphonate. Measured transition state (TS) charge changes along the trajectory suggest the creation of a new Michaelis complex (E*S, enzyme-substrate), with enhanced leaving group stabilization in the TS for the promiscuous phosphonate (betaleavinggroup from -1.08 to -0.42). Rather than altering the catalytic machinery, evolutionary repurposing was achieved by fine-tuning the molecular recognition of the phosphonate in the Michaelis complex, and by extension, also in the TS. This molecular scenario constitutes a mechanistic alternative to adaptation solely based on enzyme flexibility and conformational selection. Instead, rapid functional transitions between distinct chemical reactions rely on the high reactivity of permissive active-site architectures that allow multiple substrate binding modes.
		+
		+	Evolutionary repurposing of a sulfatase: A new Michaelis complex leads to efficient transition state charge offset.,Miton CM, Jonas S, Fischer G, Duarte F, Mohamed MF, van Loo B, Kintses B, Kamerlin SCL, Tokuriki N, Hyvonen M, Hollfelder F Proc Natl Acad Sci U S A. 2018 Jul 31;115(31):E7293-E7302. doi:, 10.1073/pnas.1607817115. Epub 2018 Jul 16. PMID:30012610<ref>PMID:30012610</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 4cxs" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 17:22, 9 March 2017

OCA — Thu, 09 Mar 2017 17:22:17 GMT

←Older revision		Revision as of 17:22, 9 March 2017
Line 1:		Line 1:
		+
	==G4 mutant of PAS, arylsulfatase from Pseudomonas Aeruginosa, in complex with Phenylphosphonic acid==		==G4 mutant of PAS, arylsulfatase from Pseudomonas Aeruginosa, in complex with Phenylphosphonic acid==
	<StructureSection load='4cxs' size='340' side='right' caption='[[4cxs]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='4cxs' size='340' side='right' caption='[[4cxs]], [[Resolution\|resolution]] 2.30Å' scene=''>
Line 7:		Line 8:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4cxk\|4cxk]], [[4cxu\|4cxu]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4cxk\|4cxk]], [[4cxu\|4cxu]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Arylsulfatase Arylsulfatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.6.1 3.1.6.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Arylsulfatase Arylsulfatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.6.1 3.1.6.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4cxs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cxs OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4cxs RCSB], [http://www.ebi.ac.uk/pdbsum/4cxs PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4cxs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cxs OCA], [http://pdbe.org/4cxs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4cxs RCSB], [http://www.ebi.ac.uk/pdbsum/4cxs PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4cxs ProSAT]</span></td></tr>
	</table>		</table>
	__TOC__		__TOC__

OCA at 12:21, 13 May 2015

OCA — Wed, 13 May 2015 12:21:28 GMT

←Older revision		Revision as of 12:21, 13 May 2015
Line 1:		Line 1:
-	'''~~Unreleased structure~~'''	+	==G4 mutant of PAS, arylsulfatase from Pseudomonas Aeruginosa, in complex with Phenylphosphonic acid==
-		+	<StructureSection load='4cxs' size='340' side='right' caption='[[4cxs]], [[Resolution\|resolution]] 2.30Å' scene=''>
-	~~The entry~~ 4cxs is ~~ON HOLD until Paper Publication~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[4cxs]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CXS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4CXS FirstGlance]. <br>
-	~~Authors~~: ~~Miton, C~~.M.~~, Jonas, S~~.~~, Mohammed, M~~.F., ~~Fischer~~, G., ~~Loo~~, B.v., ~~Kintses, B~~., ~~Hyvonen, M~~., ~~Tokuriki, N~~., ~~Hollfelder, F~~.	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=SV7:PHENYLPHOSPHONIC+ACID'>SV7</scene></td></tr>
-		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=DDZ:3,3-DIHYDROXY+L-ALANINE'>DDZ</scene></td></tr>
-	~~Description~~: ~~G4 mutant of PAS~~, ~~arylsulfatase from Pseudomonas Aeruginosa, in complex with Phenylphosphonic acid~~	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4cxk\|4cxk]], [[4cxu\|4cxu]]</td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Arylsulfatase Arylsulfatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.6.1 3.1.6.1] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4cxs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cxs OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4cxs RCSB], [http://www.ebi.ac.uk/pdbsum/4cxs PDBsum]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Arylsulfatase]]
		+	[[Category: Fischer, G]]
		+	[[Category: Hollfelder, F]]
	[[Category: Hyvonen, M]]		[[Category: Hyvonen, M]]
		+	[[Category: Jonas, S]]
	[[Category: Kintses, B]]		[[Category: Kintses, B]]
-	[[Category: ~~Hollfelder~~, F]]	+	[[Category: Loo, B v]]
-	[[Category: ~~Fischer~~, G]]	+	[[Category: Miton, C M]]
		+	[[Category: Mohammed, M F]]
	[[Category: Tokuriki, N]]		[[Category: Tokuriki, N]]
-	[[Category: ~~Loo, B.V~~]]	+	[[Category: Catalytic promiscuity]]
-	[[Category: ~~Mohammed, M.F~~]]	+	[[Category: Directed evolution]]
-	[[Category: ~~Miton, C.M~~]]	+	[[Category: Hydrolase]]
-	[[Category: ~~Jonas, S~~]]	+	[[Category: Neutral drift]]
		+	[[Category: Sulfatase]]
		+	[[Category: Superfamily]]

OCA at 13:37, 24 December 2014

OCA — Wed, 24 Dec 2014 13:37:21 GMT

←Older revision		Revision as of 13:37, 24 December 2014
Line 6:		Line 6:

	Description: G4 mutant of PAS, arylsulfatase from Pseudomonas Aeruginosa, in complex with Phenylphosphonic acid		Description: G4 mutant of PAS, arylsulfatase from Pseudomonas Aeruginosa, in complex with Phenylphosphonic acid
		+	[[Category: Unreleased Structures]]
		+	[[Category: Hyvonen, M]]
		+	[[Category: Kintses, B]]
		+	[[Category: Hollfelder, F]]
		+	[[Category: Fischer, G]]
		+	[[Category: Tokuriki, N]]
		+	[[Category: Loo, B.V]]
		+	[[Category: Mohammed, M.F]]
		+	[[Category: Miton, C.M]]
		+	[[Category: Jonas, S]]

OCA: Protected "4cxs" [edit=sysop:move=sysop]

OCA — Wed, 16 Apr 2014 08:47:04 GMT

Protected "4cxs" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:47, 16 April 2014

OCA: New page: '''Unreleased structure''' The entry 4cxs is ON HOLD until Paper Publication Authors: Miton, C.M., Jonas, S., Mohammed, M.F., Fischer, G., Loo, B.v., Kintses, B., Hyvonen, M., Tokuriki,...

OCA — Wed, 16 Apr 2014 08:47:03 GMT

New page: '''Unreleased structure''' The entry 4cxs is ON HOLD until Paper Publication Authors: Miton, C.M., Jonas, S., Mohammed, M.F., Fischer, G., Loo, B.v., Kintses, B., Hyvonen, M., Tokuriki,...

New page

'''Unreleased structure'''

The entry 4cxs is ON HOLD until Paper Publication

Authors: Miton, C.M., Jonas, S., Mohammed, M.F., Fischer, G., Loo, B.v., Kintses, B., Hyvonen, M., Tokuriki, N., Hollfelder, F.

Description: G4 mutant of PAS, arylsulfatase from Pseudomonas Aeruginosa, in complex with Phenylphosphonic acid