4d28 - Revision history

OCA at 12:19, 20 December 2023

OCA — Wed, 20 Dec 2023 12:19:39 GMT

←Older revision		Revision as of 12:19, 20 December 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4d28]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4D28 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4D28 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4d28]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4D28 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4D28 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4d28 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4d28 OCA], [https://pdbe.org/4d28 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4d28 RCSB], [https://www.ebi.ac.uk/pdbsum/4d28 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4d28 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.3Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4d28 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4d28 OCA], [https://pdbe.org/4d28 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4d28 RCSB], [https://www.ebi.ac.uk/pdbsum/4d28 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4d28 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/CIPKO_ARATH CIPKO_ARATH]] Involved in the regulatory pathway for the control of intracellular Na(+) and K(+) homeostasis and salt tolerance. Activates the vacuolar H(+)/Ca(2+) antiporter CAX1 and operates in synergy with CBL4/SOS3 to activate the plasma membrane Na(+)/H(+) antiporter SOS1. CIPK serine-threonine protein kinases interact with CBL proteins. Binding of a CBL protein to the regulatory NAF domain of CIPK protein lead to the activation of the kinase in a calcium-dependent manner. Phosphorylates CBL1, CBL4 and CBL10.<ref>PMID:10725350</ref> <ref>PMID:12034882</ref> <ref>PMID:14583601</ref> <ref>PMID:22253446</ref>	+	[https://www.uniprot.org/uniprot/CIPKO_ARATH CIPKO_ARATH] Involved in the regulatory pathway for the control of intracellular Na(+) and K(+) homeostasis and salt tolerance. Activates the vacuolar H(+)/Ca(2+) antiporter CAX1 and operates in synergy with CBL4/SOS3 to activate the plasma membrane Na(+)/H(+) antiporter SOS1. CIPK serine-threonine protein kinases interact with CBL proteins. Binding of a CBL protein to the regulatory NAF domain of CIPK protein lead to the activation of the kinase in a calcium-dependent manner. Phosphorylates CBL1, CBL4 and CBL10.<ref>PMID:10725350</ref> <ref>PMID:12034882</ref> <ref>PMID:14583601</ref> <ref>PMID:22253446</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 07:43, 14 September 2022

OCA — Wed, 14 Sep 2022 07:43:43 GMT

←Older revision		Revision as of 07:43, 14 September 2022
Line 3:		Line 3:
	<StructureSection load='4d28' size='340' side='right'caption='[[4d28]], [[Resolution\|resolution]] 3.30Å' scene=''>		<StructureSection load='4d28' size='340' side='right'caption='[[4d28]], [[Resolution\|resolution]] 3.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4d28]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Arath Arath~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4D28 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4D28 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4d28]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4D28 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4D28 FirstGlance]. <br>
-	</td></tr>~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4czt\|4czt]], [[4czu\|4czu]]</td></tr>~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4d28 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4d28 OCA], [https://pdbe.org/4d28 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4d28 RCSB], [https://www.ebi.ac.uk/pdbsum/4d28 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4d28 ProSAT]</span></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4d28 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4d28 OCA], [~~http~~://pdbe.org/4d28 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4d28 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4d28 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4d28 ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/CIPKO_ARATH CIPKO_ARATH]] Involved in the regulatory pathway for the control of intracellular Na(+) and K(+) homeostasis and salt tolerance. Activates the vacuolar H(+)/Ca(2+) antiporter CAX1 and operates in synergy with CBL4/SOS3 to activate the plasma membrane Na(+)/H(+) antiporter SOS1. CIPK serine-threonine protein kinases interact with CBL proteins. Binding of a CBL protein to the regulatory NAF domain of CIPK protein lead to the activation of the kinase in a calcium-dependent manner. Phosphorylates CBL1, CBL4 and CBL10.<ref>PMID:10725350</ref> <ref>PMID:12034882</ref> <ref>PMID:14583601</ref> <ref>PMID:22253446</ref>	+	[[https://www.uniprot.org/uniprot/CIPKO_ARATH CIPKO_ARATH]] Involved in the regulatory pathway for the control of intracellular Na(+) and K(+) homeostasis and salt tolerance. Activates the vacuolar H(+)/Ca(2+) antiporter CAX1 and operates in synergy with CBL4/SOS3 to activate the plasma membrane Na(+)/H(+) antiporter SOS1. CIPK serine-threonine protein kinases interact with CBL proteins. Binding of a CBL protein to the regulatory NAF domain of CIPK protein lead to the activation of the kinase in a calcium-dependent manner. Phosphorylates CBL1, CBL4 and CBL10.<ref>PMID:10725350</ref> <ref>PMID:12034882</ref> <ref>PMID:14583601</ref> <ref>PMID:22253446</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Arath~~]]	+	[[Category: Arabidopsis thaliana]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Non-specific serine/threonine protein kinase]]~~	+	[[Category: Albert A]]
-	[[Category: Albert, A]]	+	[[Category: Chaves-Sanjuan A]]
-	[[Category: Chaves-Sanjuan, A]]	+	[[Category: Gonzalez-Rubio JM]]
-	[[Category: Gonzalez-Rubio~~, J M~~]]	+	[[Category: Sanchez-Barrena MJ]]
-	[[Category: Sanchez-Barrena~~, M J]]~~	+
-	~~[[Category: Ion homeostasis]]~~	+
-	~~[[Category: Salt stress]]~~	+
-	~~[[Category: Sodium transport]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 09:18, 5 February 2020

OCA — Wed, 05 Feb 2020 09:18:55 GMT

←Older revision		Revision as of 09:18, 5 February 2020
Line 1:		Line 1:

	==Crystal structure of the kinase domain of CIPK24/SOS2==		==Crystal structure of the kinase domain of CIPK24/SOS2==
-	<StructureSection load='4d28' size='340' side='right' caption='[[4d28]], [[Resolution\|resolution]] 3.30Å' scene=''>	+	<StructureSection load='4d28' size='340' side='right'caption='[[4d28]], [[Resolution\|resolution]] 3.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4d28]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4D28 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4D28 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4d28]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4D28 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4D28 FirstGlance]. <br>
Line 24:		Line 24:
	</StructureSection>		</StructureSection>
	[[Category: Arath]]		[[Category: Arath]]
		+	[[Category: Large Structures]]
	[[Category: Non-specific serine/threonine protein kinase]]		[[Category: Non-specific serine/threonine protein kinase]]
	[[Category: Albert, A]]		[[Category: Albert, A]]

OCA at 07:29, 6 December 2017

OCA — Wed, 06 Dec 2017 07:29:18 GMT

←Older revision		Revision as of 07:29, 6 December 2017
Line 1:		Line 1:

-	==Crystal structure of the kinase domain of ~~SnRK3.11 At5g35410~~==	+	==Crystal structure of the kinase domain of CIPK24/SOS2==
	<StructureSection load='4d28' size='340' side='right' caption='[[4d28]], [[Resolution\|resolution]] 3.30Å' scene=''>		<StructureSection load='4d28' size='340' side='right' caption='[[4d28]], [[Resolution\|resolution]] 3.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4d28]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4D28 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4D28 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4d28]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4D28 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4D28 FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4czt\|4czt]], [[4czu\|4czu]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4czt\|4czt]], [[4czu\|4czu]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] </span></td></tr>
Line 23:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Arath]]
	[[Category: Non-specific serine/threonine protein kinase]]		[[Category: Non-specific serine/threonine protein kinase]]
	[[Category: Albert, A]]		[[Category: Albert, A]]

OCA at 10:57, 4 August 2016

OCA — Thu, 04 Aug 2016 10:57:29 GMT

←Older revision		Revision as of 10:57, 4 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of the kinase domain of SnRK3.11 At5g35410==		==Crystal structure of the kinase domain of SnRK3.11 At5g35410==
	<StructureSection load='4d28' size='340' side='right' caption='[[4d28]], [[Resolution\|resolution]] 3.30Å' scene=''>		<StructureSection load='4d28' size='340' side='right' caption='[[4d28]], [[Resolution\|resolution]] 3.30Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4czt\|4czt]], [[4czu\|4czu]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4czt\|4czt]], [[4czu\|4czu]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4d28 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4d28 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4d28 RCSB], [http://www.ebi.ac.uk/pdbsum/4d28 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4d28 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4d28 OCA], [http://pdbe.org/4d28 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4d28 RCSB], [http://www.ebi.ac.uk/pdbsum/4d28 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4d28 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4d28" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 13:34, 24 December 2014

OCA — Wed, 24 Dec 2014 13:34:24 GMT

←Older revision		Revision as of 13:34, 24 December 2014
Line 7:		Line 7:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4d28 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4d28 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4d28 RCSB], [http://www.ebi.ac.uk/pdbsum/4d28 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4d28 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4d28 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4d28 RCSB], [http://www.ebi.ac.uk/pdbsum/4d28 PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/CIPKO_ARATH CIPKO_ARATH]] Involved in the regulatory pathway for the control of intracellular Na(+) and K(+) homeostasis and salt tolerance. Activates the vacuolar H(+)/Ca(2+) antiporter CAX1 and operates in synergy with CBL4/SOS3 to activate the plasma membrane Na(+)/H(+) antiporter SOS1. CIPK serine-threonine protein kinases interact with CBL proteins. Binding of a CBL protein to the regulatory NAF domain of CIPK protein lead to the activation of the kinase in a calcium-dependent manner. Phosphorylates CBL1, CBL4 and CBL10.<ref>PMID:10725350</ref> <ref>PMID:12034882</ref> <ref>PMID:14583601</ref> <ref>PMID:22253446</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 08:32, 17 December 2014

OCA — Wed, 17 Dec 2014 08:32:50 GMT

←Older revision		Revision as of 08:32, 17 December 2014
Line 20:		Line 20:
	</StructureSection>		</StructureSection>
	[[Category: Non-specific serine/threonine protein kinase]]		[[Category: Non-specific serine/threonine protein kinase]]
-	[[Category: Albert, A.]]	+	[[Category: Albert, A]]
-	[[Category: Chaves-Sanjuan, A.]]	+	[[Category: Chaves-Sanjuan, A]]
-	[[Category: Gonzalez-Rubio, J M.]]	+	[[Category: Gonzalez-Rubio, J M]]
-	[[Category: Sanchez-Barrena, M J.]]	+	[[Category: Sanchez-Barrena, M J]]
	[[Category: Ion homeostasis]]		[[Category: Ion homeostasis]]
	[[Category: Salt stress]]		[[Category: Salt stress]]
	[[Category: Sodium transport]]		[[Category: Sodium transport]]
	[[Category: Transferase]]		[[Category: Transferase]]

OCA at 10:38, 20 October 2014

OCA — Mon, 20 Oct 2014 10:38:05 GMT

←Older revision		Revision as of 10:38, 20 October 2014
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==Crystal structure of the kinase domain of SnRK3.11 At5g35410==
		+	<StructureSection load='4d28' size='340' side='right' caption='[[4d28]], [[Resolution\|resolution]] 3.30Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4d28]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4D28 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4D28 FirstGlance]. <br>
		+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4czt\|4czt]], [[4czu\|4czu]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4d28 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4d28 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4d28 RCSB], [http://www.ebi.ac.uk/pdbsum/4d28 PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Plant cells have developed specific protective molecular machinery against environmental stresses. The family of CBL-interacting protein kinases (CIPK) and their interacting activators, the calcium sensors calcineurin B-like (CBLs), work together to decode calcium signals elicited by stress situations. The molecular basis of biological activation of CIPKs relies on the calcium-dependent interaction of a self-inhibitory NAF motif with a particular CBL, the phosphorylation of the activation loop by upstream kinases, and the subsequent phosphorylation of the CBL by the CIPK. We present the crystal structures of the NAF-truncated and pseudophosphorylated kinase domains of CIPK23 and CIPK24/SOS2. In addition, we provide biochemical data showing that although CIPK23 is intrinsically inactive and requires an external stimulation, CIPK24/SOS2 displays basal activity. This data correlates well with the observed conformation of the respective activation loops: Although the loop of CIPK23 is folded into a well-ordered structure that blocks the active site access to substrates, the loop of CIPK24/SOS2 protrudes out of the active site and allows catalysis. These structures together with biochemical and biophysical data show that CIPK kinase activity necessarily requires the coordinated releases of the activation loop from the active site and of the NAF motif from the nucleotide-binding site. Taken all together, we postulate the basis for a conserved calcium-dependent NAF-mediated regulation of CIPKs and a variable regulation by upstream kinases.

-	~~The entry 4d28 is ON HOLD until Paper Publication~~	+	Structural basis of the regulatory mechanism of the plant CIPK family of protein kinases controlling ion homeostasis and abiotic stress.,Chaves-Sanjuan A, Sanchez-Barrena MJ, Gonzalez-Rubio JM, Moreno M, Ragel P, Jimenez M, Pardo JM, Martinez-Ripoll M, Quintero FJ, Albert A Proc Natl Acad Sci U S A. 2014 Oct 6. pii: 201407610. PMID:25288725<ref>PMID:25288725</ref>

-	~~Authors: Gonzalez-Rubio~~, J.M., Chaves-Sanjuan, A., Sanchez-Barrena, M.J.~~, Albert, A.~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description~~: ~~Crystal structure of the kinase domain of SnRK3.11 At5g35410~~	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Non-specific serine/threonine protein kinase]]
		+	[[Category: Albert, A.]]
		+	[[Category: Chaves-Sanjuan, A.]]
		+	[[Category: Gonzalez-Rubio, J M.]]
		+	[[Category: Sanchez-Barrena, M J.]]
		+	[[Category: Ion homeostasis]]
		+	[[Category: Salt stress]]
		+	[[Category: Sodium transport]]
		+	[[Category: Transferase]]

OCA: Protected "4d28" [edit=sysop:move=sysop]

OCA — Wed, 14 May 2014 07:02:50 GMT

Protected "4d28" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:02, 14 May 2014

OCA: New page: '''Unreleased structure''' The entry 4d28 is ON HOLD until Paper Publication Authors: Gonzalez-Rubio, J.M., Chaves-Sanjuan, A., Sanchez-Barrena, M.J., Albert, A. Description: Crystal s...

OCA — Wed, 14 May 2014 07:02:49 GMT

New page: '''Unreleased structure''' The entry 4d28 is ON HOLD until Paper Publication Authors: Gonzalez-Rubio, J.M., Chaves-Sanjuan, A., Sanchez-Barrena, M.J., Albert, A. Description: Crystal s...

New page

'''Unreleased structure'''

The entry 4d28 is ON HOLD until Paper Publication

Authors: Gonzalez-Rubio, J.M., Chaves-Sanjuan, A., Sanchez-Barrena, M.J., Albert, A.

Description: Crystal structure of the kinase domain of SnRK3.11 At5g35410