4d5v - Revision history

OCA at 12:21, 20 December 2023

OCA — Wed, 20 Dec 2023 12:21:16 GMT

←Older revision		Revision as of 12:21, 20 December 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4d5v]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Trichoderma_reesei Trichoderma reesei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4D5V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4D5V FirstGlance]. <br>		<table><tr><td colspan='2'>[[4d5v]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Trichoderma_reesei Trichoderma reesei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4D5V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4D5V FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene>, <scene name='pdbligand=XLS:D-XYLOSE+(LINEAR+FORM)'>XLS</scene>, <scene name='pdbligand=XYP:BETA-D-XYLOPYRANOSE'>XYP</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.62Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene>, <scene name='pdbligand=XLS:D-XYLOSE+(LINEAR+FORM)'>XLS</scene>, <scene name='pdbligand=XYP:BETA-D-XYLOPYRANOSE'>XYP</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4d5v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4d5v OCA], [https://pdbe.org/4d5v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4d5v RCSB], [https://www.ebi.ac.uk/pdbsum/4d5v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4d5v ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4d5v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4d5v OCA], [https://pdbe.org/4d5v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4d5v RCSB], [https://www.ebi.ac.uk/pdbsum/4d5v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4d5v ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/GUX1_HYPJE GUX1_HYPJE]] The biological conversion of cellulose to glucose generally requires three types of hydrolytic enzymes: (1) Endoglucanases which cut internal beta-1,4-glucosidic bonds; (2) Exocellobiohydrolases that cut the dissaccharide cellobiose from the non-reducing end of the cellulose polymer chain; (3) Beta-1,4-glucosidases which hydrolyze the cellobiose and other short cello-oligosaccharides to glucose.	+	[https://www.uniprot.org/uniprot/GUX1_HYPJE GUX1_HYPJE] The biological conversion of cellulose to glucose generally requires three types of hydrolytic enzymes: (1) Endoglucanases which cut internal beta-1,4-glucosidic bonds; (2) Exocellobiohydrolases that cut the dissaccharide cellobiose from the non-reducing end of the cellulose polymer chain; (3) Beta-1,4-glucosidases which hydrolyze the cellobiose and other short cello-oligosaccharides to glucose.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 07:48, 14 September 2022

OCA — Wed, 14 Sep 2022 07:48:48 GMT

←Older revision		Revision as of 07:48, 14 September 2022
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	<StructureSection load='4d5v' size='340' side='right'caption='[[4d5v]], [[Resolution\|resolution]] 1.62Å' scene=''>		<StructureSection load='4d5v' size='340' side='right'caption='[[4d5v]], [[Resolution\|resolution]] 1.62Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4d5v]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Atcc_13631 Atcc 13631~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4D5V OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4D5V FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4d5v]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Trichoderma_reesei Trichoderma reesei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4D5V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4D5V FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=XLS:D-XYLOSE+(LINEAR+FORM)'>XLS</scene>, <scene name='pdbligand=XYP:BETA-D-XYLOPYRANOSE'>XYP</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene>, <scene name='pdbligand=XLS:D-XYLOSE+(LINEAR+FORM)'>XLS</scene>, <scene name='pdbligand=XYP:BETA-D-XYLOPYRANOSE'>XYP</scene></td></tr>
-	~~<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4d5v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4d5v OCA], [https://pdbe.org/4d5v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4d5v RCSB], [https://www.ebi.ac.uk/pdbsum/4d5v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4d5v ProSAT]</span></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4d5i\|4d5i]], [[4d5j\|4d5j]], [[4d5o\|4d5o]], [[4d5p\|4d5p]], [[4d5q\|4d5q]]</td></tr>~~	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cellulose_1,4-beta-cellobiosidase_(reducing_end) Cellulose 1,4-beta-cellobiosidase (reducing end)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.176 3.2.1.176] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4d5v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4d5v OCA], [~~http~~://pdbe.org/4d5v PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4d5v RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4d5v PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4d5v ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/GUX1_HYPJE GUX1_HYPJE]] The biological conversion of cellulose to glucose generally requires three types of hydrolytic enzymes: (1) Endoglucanases which cut internal beta-1,4-glucosidic bonds; (2) Exocellobiohydrolases that cut the dissaccharide cellobiose from the non-reducing end of the cellulose polymer chain; (3) Beta-1,4-glucosidases which hydrolyze the cellobiose and other short cello-oligosaccharides to glucose.	+	[[https://www.uniprot.org/uniprot/GUX1_HYPJE GUX1_HYPJE]] The biological conversion of cellulose to glucose generally requires three types of hydrolytic enzymes: (1) Endoglucanases which cut internal beta-1,4-glucosidic bonds; (2) Exocellobiohydrolases that cut the dissaccharide cellobiose from the non-reducing end of the cellulose polymer chain; (3) Beta-1,4-glucosidases which hydrolyze the cellobiose and other short cello-oligosaccharides to glucose.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Atcc 13631]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Hansson, H~~]]	+	[[Category: Trichoderma reesei]]
-	[[Category: ~~Momeni, M~~ H]]	+	[[Category: Hansson H]]
-	[[Category: ~~Stahlberg, J~~]]	+	[[Category: Momeni MH]]
-	[[Category: ~~Cellobiohydrolase]]~~	+	[[Category: Stahlberg J]]
-	~~[[Category: Cellulase. inhibition]]~~	+
-	~~[[Category: Glycoside hydrolase]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Xylooligosaccharide~~]]	+

OCA at 12:52, 10 May 2019

OCA — Fri, 10 May 2019 12:52:41 GMT

←Older revision		Revision as of 12:52, 10 May 2019
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	==Hypocrea jecorina cellobiohydrolase Cel7A E217Q soaked with xylotetraose.==		==Hypocrea jecorina cellobiohydrolase Cel7A E217Q soaked with xylotetraose.==
-	<StructureSection load='4d5v' size='340' side='right' caption='[[4d5v]], [[Resolution\|resolution]] 1.62Å' scene=''>	+	<StructureSection load='4d5v' size='340' side='right'caption='[[4d5v]], [[Resolution\|resolution]] 1.62Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4d5v]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_13631 Atcc 13631]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4D5V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4D5V FirstGlance]. <br>		<table><tr><td colspan='2'>[[4d5v]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_13631 Atcc 13631]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4D5V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4D5V FirstGlance]. <br>
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	</div>		</div>
	<div class="pdbe-citations 4d5v" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4d5v" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Cellobiohydrolase 3D structures\|Cellobiohydrolase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 26:		Line 29:
	</StructureSection>		</StructureSection>
	[[Category: Atcc 13631]]		[[Category: Atcc 13631]]
		+	[[Category: Large Structures]]
	[[Category: Hansson, H]]		[[Category: Hansson, H]]
	[[Category: Momeni, M H]]		[[Category: Momeni, M H]]

OCA at 07:52, 17 January 2018

OCA — Wed, 17 Jan 2018 07:52:43 GMT

←Older revision		Revision as of 07:52, 17 January 2018
Line 3:		Line 3:
	<StructureSection load='4d5v' size='340' side='right' caption='[[4d5v]], [[Resolution\|resolution]] 1.62Å' scene=''>		<StructureSection load='4d5v' size='340' side='right' caption='[[4d5v]], [[Resolution\|resolution]] 1.62Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4d5v]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4D5V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4D5V FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4d5v]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_13631 Atcc 13631]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4D5V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4D5V FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=XLS:D-XYLOSE+(LINEAR+FORM)'>XLS</scene>, <scene name='pdbligand=XYP:BETA-D-XYLOPYRANOSE'>XYP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=XLS:D-XYLOSE+(LINEAR+FORM)'>XLS</scene>, <scene name='pdbligand=XYP:BETA-D-XYLOPYRANOSE'>XYP</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Atcc 13631]]
	[[Category: Hansson, H]]		[[Category: Hansson, H]]
	[[Category: Momeni, M H]]		[[Category: Momeni, M H]]

OCA at 17:07, 22 March 2017

OCA — Wed, 22 Mar 2017 17:07:21 GMT

←Older revision		Revision as of 17:07, 22 March 2017
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		+
	==Hypocrea jecorina cellobiohydrolase Cel7A E217Q soaked with xylotetraose.==		==Hypocrea jecorina cellobiohydrolase Cel7A E217Q soaked with xylotetraose.==
	<StructureSection load='4d5v' size='340' side='right' caption='[[4d5v]], [[Resolution\|resolution]] 1.62Å' scene=''>		<StructureSection load='4d5v' size='340' side='right' caption='[[4d5v]], [[Resolution\|resolution]] 1.62Å' scene=''>
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	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4d5i\|4d5i]], [[4d5j\|4d5j]], [[4d5o\|4d5o]], [[4d5p\|4d5p]], [[4d5q\|4d5q]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4d5i\|4d5i]], [[4d5j\|4d5j]], [[4d5o\|4d5o]], [[4d5p\|4d5p]], [[4d5q\|4d5q]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cellulose_1,4-beta-cellobiosidase_(reducing_end) Cellulose 1,4-beta-cellobiosidase (reducing end)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.176 3.2.1.176] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cellulose_1,4-beta-cellobiosidase_(reducing_end) Cellulose 1,4-beta-cellobiosidase (reducing end)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.176 3.2.1.176] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4d5v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4d5v OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4d5v RCSB], [http://www.ebi.ac.uk/pdbsum/4d5v PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4d5v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4d5v OCA], [http://pdbe.org/4d5v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4d5v RCSB], [http://www.ebi.ac.uk/pdbsum/4d5v PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4d5v ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 19:		Line 20:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4d5v" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 14:02, 26 March 2015

OCA — Thu, 26 Mar 2015 14:02:09 GMT

←Older revision		Revision as of 14:02, 26 March 2015
Line 1:		Line 1:
-	'''~~Unreleased~~ structure'''	+	==Hypocrea jecorina cellobiohydrolase Cel7A E217Q soaked with xylotetraose.==
		+	<StructureSection load='4d5v' size='340' side='right' caption='[[4d5v]], [[Resolution\|resolution]] 1.62Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4d5v]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4D5V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4D5V FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=XLS:D-XYLOSE+(LINEAR+FORM)'>XLS</scene>, <scene name='pdbligand=XYP:BETA-D-XYLOPYRANOSE'>XYP</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4d5i\|4d5i]], [[4d5j\|4d5j]], [[4d5o\|4d5o]], [[4d5p\|4d5p]], [[4d5q\|4d5q]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cellulose_1,4-beta-cellobiosidase_(reducing_end) Cellulose 1,4-beta-cellobiosidase (reducing end)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.176 3.2.1.176] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4d5v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4d5v OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4d5v RCSB], [http://www.ebi.ac.uk/pdbsum/4d5v PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/GUX1_HYPJE GUX1_HYPJE]] The biological conversion of cellulose to glucose generally requires three types of hydrolytic enzymes: (1) Endoglucanases which cut internal beta-1,4-glucosidic bonds; (2) Exocellobiohydrolases that cut the dissaccharide cellobiose from the non-reducing end of the cellulose polymer chain; (3) Beta-1,4-glucosidases which hydrolyze the cellobiose and other short cello-oligosaccharides to glucose.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The filamentous fungus Hypocrea jecorina (anamorph to Trichoderma reesei) is the predominant source of enzymes for industrial saccharification of lignocellulose biomass. The major enzyme, cellobiohydrolase Cel7A, constitutes nearly half of the total protein in the secretome. The performance of the enzymes is susceptible to inhibition by compounds liberated by physico-chemical pretreatment if the biomass is kept unwashed. Xylan and xylooligosaccharides (XOS) have been proposed to play a key role for inhibition of cellobiohydrolases of glycoside hydrolase family 7. To elucidate the mechanism behind this inhibition at a molecular level, we have used X-ray crystallography to determine structures of H. jecorina Cel7A in complex with XOS. Structures with xylotriose, -tetraose, and -pentaose, reveal a predominant binding mode at the beginning of the substrate-binding tunnel of the enzyme, where each xylose residue is shifted about 2.4 A towards the catalytic center compared to the binding of cellooligosaccharides. Furthermore, partial occupancy of two consecutive xylose residues in subsites -2 and -1 suggests an alternate binding mode for XOS in the vicinity of the catalytic center. Interestingly, the -1 xylosyl unit exhibits an open aldehyde conformation in one of the structures and a regular ring-closed pyranoside in another complex. Complementary inhibition studies with p-nitrophenyl lactoside as substrate indicate mixed inhibition rather than pure competitive inhibition. This article is protected by copyright. All rights reserved.

-	~~The entry 4d5v is ON HOLD until Paper Publication~~	+	Structural insights into the inhibition of Cellobiohydrolase Cel7A by xylooligosaccharides.,Haddad Momeni M, Ubhayasekera W, Sandgren M, Stahlberg J, Hansson H FEBS J. 2015 Mar 12. doi: 10.1111/febs.13265. PMID:25765184<ref>PMID:25765184</ref>

-	~~Authors: Momeni~~, M.H.~~, Stahlberg, J., Hansson, H~~.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~Description: Hypocrea jecorina cellobiohydrolase Cel7A E217Q soaked with xylotetraose.~~	+	== References ==
-	~~[[Category: Unreleased Structures]]~~	+	<references/>
-	~~[[Category: Momeni, M.H]]~~	+	__TOC__
		+	</StructureSection>
	[[Category: Hansson, H]]		[[Category: Hansson, H]]
		+	[[Category: Momeni, M H]]
	[[Category: Stahlberg, J]]		[[Category: Stahlberg, J]]
		+	[[Category: Cellobiohydrolase]]
		+	[[Category: Cellulase. inhibition]]
		+	[[Category: Glycoside hydrolase]]
		+	[[Category: Hydrolase]]
		+	[[Category: Xylooligosaccharide]]

OCA at 13:52, 24 December 2014

OCA — Wed, 24 Dec 2014 13:52:20 GMT

←Older revision		Revision as of 13:52, 24 December 2014
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	Description: Hypocrea jecorina cellobiohydrolase Cel7A E217Q soaked with xylotetraose.		Description: Hypocrea jecorina cellobiohydrolase Cel7A E217Q soaked with xylotetraose.
		+	[[Category: Unreleased Structures]]
		+	[[Category: Momeni, M.H]]
		+	[[Category: Hansson, H]]
		+	[[Category: Stahlberg, J]]

OCA at 06:26, 26 November 2014

OCA — Wed, 26 Nov 2014 06:26:36 GMT

←Older revision		Revision as of 06:26, 26 November 2014
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4d5v is ON HOLD	+	The entry 4d5v is ON HOLD until Paper Publication

	Authors: Momeni, M.H., Stahlberg, J., Hansson, H.		Authors: Momeni, M.H., Stahlberg, J., Hansson, H.

	Description: Hypocrea jecorina cellobiohydrolase Cel7A E217Q soaked with xylotetraose.		Description: Hypocrea jecorina cellobiohydrolase Cel7A E217Q soaked with xylotetraose.

OCA: Protected "4d5v" [edit=sysop:move=sysop]

OCA — Wed, 19 Nov 2014 11:08:06 GMT

Protected "4d5v" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:08, 19 November 2014

OCA: New page: '''Unreleased structure''' The entry 4d5v is ON HOLD Authors: Momeni, M.H., Stahlberg, J., Hansson, H. Description: Hypocrea jecorina cellobiohydrolase Cel7A E217Q soaked with xylotetr...

OCA — Wed, 19 Nov 2014 11:08:05 GMT

New page: '''Unreleased structure''' The entry 4d5v is ON HOLD Authors: Momeni, M.H., Stahlberg, J., Hansson, H. Description: Hypocrea jecorina cellobiohydrolase Cel7A E217Q soaked with xylotetr...

New page

'''Unreleased structure'''

The entry 4d5v is ON HOLD

Authors: Momeni, M.H., Stahlberg, J., Hansson, H.

Description: Hypocrea jecorina cellobiohydrolase Cel7A E217Q soaked with xylotetraose.