4dhx - Revision history

OCA at 10:48, 1 March 2024

OCA — Fri, 01 Mar 2024 10:48:37 GMT

←Older revision		Revision as of 10:48, 1 March 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4dhx]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DHX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4DHX FirstGlance]. <br>		<table><tr><td colspan='2'>[[4dhx]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DHX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4DHX FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4dhx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dhx OCA], [https://pdbe.org/4dhx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4dhx RCSB], [https://www.ebi.ac.uk/pdbsum/4dhx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4dhx ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.1Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4dhx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dhx OCA], [https://pdbe.org/4dhx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4dhx RCSB], [https://www.ebi.ac.uk/pdbsum/4dhx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4dhx ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/GANP_HUMAN GANP_HUMAN]] Isoform GANP: Essential for the generation of high-affinity B-cells against T-cell-dependent antigens by affecting somatic hypermutation at the IgV-regions. May have stimulation-dependent DNA primase activity that would generate extra RNA primers in very rapidely proliferating cells and would support clonal expansion of differentiating B-cells (By similarity). Involved in the nuclear export of poly(A)-containing mRNAs by acting as a scaffold for the TREX-2 complex. The TREX-2 complex functions in docking export-competent ribonucleoprotein particles (mRNPs) to the nuclear entrance of the nuclear pore complex (nuclear basket). TREX-2 participates in mRNA export and accurate chromatin positioning in the nucleus by tethering genes to the nuclear periphery.<ref>PMID:22307388</ref> Isoform MCM3AP: Acetyltransferase targeting MCM3. Inhibits initiation of DNA replication, but not elongation.<ref>PMID:22307388</ref>	+	[https://www.uniprot.org/uniprot/GANP_HUMAN GANP_HUMAN] Isoform GANP: Essential for the generation of high-affinity B-cells against T-cell-dependent antigens by affecting somatic hypermutation at the IgV-regions. May have stimulation-dependent DNA primase activity that would generate extra RNA primers in very rapidely proliferating cells and would support clonal expansion of differentiating B-cells (By similarity). Involved in the nuclear export of poly(A)-containing mRNAs by acting as a scaffold for the TREX-2 complex. The TREX-2 complex functions in docking export-competent ribonucleoprotein particles (mRNPs) to the nuclear entrance of the nuclear pore complex (nuclear basket). TREX-2 participates in mRNA export and accurate chromatin positioning in the nucleus by tethering genes to the nuclear periphery.<ref>PMID:22307388</ref> Isoform MCM3AP: Acetyltransferase targeting MCM3. Inhibits initiation of DNA replication, but not elongation.<ref>PMID:22307388</ref>
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	Export of messenger RNA (mRNA) from the nucleus to the cytoplasm is a critical step in the gene expression pathway of eukaryotic cells. Here, we report the functional and structural characterization of the mammalian TREX-2 complex and show how it links transcription/processing with nuclear mRNA export. Mammalian TREX-2 is based on a germinal-centre associated nuclear protein (GANP) scaffold to which ENY2, PCID2 and centrins bind and depletion of any of these components inhibits mRNA export. The crystal structure of the GANP:ENY2 complex shows that two ENY2 chains interact directly with GANP, but they have different orientations from those observed on yeast Sac3. GANP is required to recruit ENY2 to nuclear pore complexes (NPCs), but ENY2 is not necessary to recruit GANP, which requires both its CID and MCM3AP domains, together with nucleoporin Nup153. GANP and ENY2 associate with RNA polymerase II and inhibition of mRNA processing redistributes GANP from NPCs into nuclear foci indicating that mammalian TREX-2 is associated with transcription. Thus, we implicate TREX-2 as an integral component of the mammalian mRNA export machinery where it links transcription and nuclear export by facilitating the transfer of mature mRNPs from the nuclear interior to NPCs.	+
-		+
-	Functional and structural characterization of the mammalian TREX-2 complex that links transcription with nuclear messenger RNA export.,Jani D, Lutz S, Hurt E, Laskey RA, Stewart M, Wickramasinghe VO Nucleic Acids Res. 2012 May 1;40(10):4562-73. Epub 2012 Feb 4. PMID:22307388<ref>PMID:22307388</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 4dhx" style="background-color:#fffaf0;"></div~~>	+
	== References ==		== References ==
	<references/>		<references/>

OCA at 08:19, 21 September 2022

OCA — Wed, 21 Sep 2022 08:19:03 GMT

←Older revision		Revision as of 08:19, 21 September 2022
Line 1:		Line 1:

	==ENY2:GANP complex==		==ENY2:GANP complex==
-	<StructureSection load='4dhx' size='340' side='right' caption='[[4dhx]], [[Resolution\|resolution]] 2.10Å' scene=''>	+	<StructureSection load='4dhx' size='340' side='right'caption='[[4dhx]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4dhx]] is a 6 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DHX OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4DHX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4dhx]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DHX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4DHX FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MCM3AP, GANP, KIAA0572, MAP80 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), ENY2, DC6 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4dhx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dhx OCA], [https://pdbe.org/4dhx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4dhx RCSB], [https://www.ebi.ac.uk/pdbsum/4dhx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4dhx ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4dhx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dhx OCA], [~~http~~://pdbe.org/4dhx PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4dhx RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4dhx PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4dhx ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/GANP_HUMAN GANP_HUMAN]] Isoform GANP: Essential for the generation of high-affinity B-cells against T-cell-dependent antigens by affecting somatic hypermutation at the IgV-regions. May have stimulation-dependent DNA primase activity that would generate extra RNA primers in very rapidely proliferating cells and would support clonal expansion of differentiating B-cells (By similarity). Involved in the nuclear export of poly(A)-containing mRNAs by acting as a scaffold for the TREX-2 complex. The TREX-2 complex functions in docking export-competent ribonucleoprotein particles (mRNPs) to the nuclear entrance of the nuclear pore complex (nuclear basket). TREX-2 participates in mRNA export and accurate chromatin positioning in the nucleus by tethering genes to the nuclear periphery.<ref>PMID:22307388</ref> Isoform MCM3AP: Acetyltransferase targeting MCM3. Inhibits initiation of DNA replication, but not elongation.<ref>PMID:22307388</ref> [[http://www.uniprot.org/uniprot/ENY2_HUMAN ENY2_HUMAN]] Involved in mRNA export coupled transcription activation by association with both the TREX-2 and the SAGA complexes. The transcription regulatory histone acetylation (HAT) complex SAGA is a multiprotein complex that activates transcription by remodeling chromatin and mediating histone acetylation and deubiquitination. Within the SAGA complex, participates in a subcomplex that specifically deubiquitinates both histones H2A and H2B. The SAGA complex is recruited to specific gene promoters by activators such as MYC, where it is required for transcription. Required for nuclear receptor-mediated transactivation. The TREX-2 complex functions in docking export-competent ribonucleoprotein particles (mRNPs) to the nuclear entrance of the nuclear pore complex (nuclear basket). TREX-2 participates in mRNA export and accurate chromatin positioning in the nucleus by tethering genes to the nuclear periphery.<ref>PMID:18206972</ref> <ref>PMID:21746879</ref>	+	[[https://www.uniprot.org/uniprot/GANP_HUMAN GANP_HUMAN]] Isoform GANP: Essential for the generation of high-affinity B-cells against T-cell-dependent antigens by affecting somatic hypermutation at the IgV-regions. May have stimulation-dependent DNA primase activity that would generate extra RNA primers in very rapidely proliferating cells and would support clonal expansion of differentiating B-cells (By similarity). Involved in the nuclear export of poly(A)-containing mRNAs by acting as a scaffold for the TREX-2 complex. The TREX-2 complex functions in docking export-competent ribonucleoprotein particles (mRNPs) to the nuclear entrance of the nuclear pore complex (nuclear basket). TREX-2 participates in mRNA export and accurate chromatin positioning in the nucleus by tethering genes to the nuclear periphery.<ref>PMID:22307388</ref> Isoform MCM3AP: Acetyltransferase targeting MCM3. Inhibits initiation of DNA replication, but not elongation.<ref>PMID:22307388</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
-	[[Category: ~~Jani, D~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Stewart, M~~]]	+	[[Category: Jani D]]
-	[[Category: ~~Mrna export]]~~	+	[[Category: Stewart M]]
-	~~[[Category: Transport protein-dna binding protein complex~~]]	+

OCA at 07:52, 4 August 2016

OCA — Thu, 04 Aug 2016 07:52:50 GMT

←Older revision		Revision as of 07:52, 4 August 2016
Line 1:		Line 1:
		+
	==ENY2:GANP complex==		==ENY2:GANP complex==
	<StructureSection load='4dhx' size='340' side='right' caption='[[4dhx]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='4dhx' size='340' side='right' caption='[[4dhx]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4dhx]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DHX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4DHX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4dhx]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DHX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4DHX FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MCM3AP, GANP, KIAA0572, MAP80 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 ~~Homo sapiens~~]), ENY2, DC6 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 ~~Homo sapiens~~])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MCM3AP, GANP, KIAA0572, MAP80 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), ENY2, DC6 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4dhx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dhx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4dhx RCSB], [http://www.ebi.ac.uk/pdbsum/4dhx PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4dhx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dhx OCA], [http://pdbe.org/4dhx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4dhx RCSB], [http://www.ebi.ac.uk/pdbsum/4dhx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4dhx ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[http://www.uniprot.org/uniprot/ENY2_HUMAN ENY2_HUMAN]] Involved in mRNA export coupled transcription activation by association with both the TREX-2 and the SAGA complexes. The transcription regulatory histone acetylation (HAT) complex SAGA is a multiprotein complex that activates transcription by remodeling chromatin and mediating histone acetylation and deubiquitination. Within the SAGA complex, participates in a subcomplex that specifically deubiquitinates both histones H2A and H2B. The SAGA complex is recruited to specific gene promoters by activators such as MYC, where it is required for transcription. Required for nuclear receptor-mediated transactivation. The TREX-2 complex functions in docking export-competent ribonucleoprotein particles (mRNPs) to the nuclear entrance of the nuclear pore complex (nuclear basket). TREX-2 participates in mRNA export and accurate chromatin positioning in the nucleus by tethering genes to the nuclear periphery.<ref>PMID:18206972</ref> <ref>PMID:21746879</ref>	+	[[http://www.uniprot.org/uniprot/GANP_HUMAN GANP_HUMAN]] Isoform GANP: Essential for the generation of high-affinity B-cells against T-cell-dependent antigens by affecting somatic hypermutation at the IgV-regions. May have stimulation-dependent DNA primase activity that would generate extra RNA primers in very rapidely proliferating cells and would support clonal expansion of differentiating B-cells (By similarity). Involved in the nuclear export of poly(A)-containing mRNAs by acting as a scaffold for the TREX-2 complex. The TREX-2 complex functions in docking export-competent ribonucleoprotein particles (mRNPs) to the nuclear entrance of the nuclear pore complex (nuclear basket). TREX-2 participates in mRNA export and accurate chromatin positioning in the nucleus by tethering genes to the nuclear periphery.<ref>PMID:22307388</ref> Isoform MCM3AP: Acetyltransferase targeting MCM3. Inhibits initiation of DNA replication, but not elongation.<ref>PMID:22307388</ref> [[http://www.uniprot.org/uniprot/ENY2_HUMAN ENY2_HUMAN]] Involved in mRNA export coupled transcription activation by association with both the TREX-2 and the SAGA complexes. The transcription regulatory histone acetylation (HAT) complex SAGA is a multiprotein complex that activates transcription by remodeling chromatin and mediating histone acetylation and deubiquitination. Within the SAGA complex, participates in a subcomplex that specifically deubiquitinates both histones H2A and H2B. The SAGA complex is recruited to specific gene promoters by activators such as MYC, where it is required for transcription. Required for nuclear receptor-mediated transactivation. The TREX-2 complex functions in docking export-competent ribonucleoprotein particles (mRNPs) to the nuclear entrance of the nuclear pore complex (nuclear basket). TREX-2 participates in mRNA export and accurate chromatin positioning in the nucleus by tethering genes to the nuclear periphery.<ref>PMID:18206972</ref> <ref>PMID:21746879</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4dhx" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Homo sapiens~~]]	+	[[Category: Human]]
	[[Category: Jani, D]]		[[Category: Jani, D]]
	[[Category: Stewart, M]]		[[Category: Stewart, M]]
	[[Category: Mrna export]]		[[Category: Mrna export]]
	[[Category: Transport protein-dna binding protein complex]]		[[Category: Transport protein-dna binding protein complex]]

OCA at 07:14, 24 December 2014

OCA — Wed, 24 Dec 2014 07:14:44 GMT

←Older revision		Revision as of 07:14, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4dhx]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DHX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4DHX FirstGlance]. <br>		<table><tr><td colspan='2'>[[4dhx]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DHX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4DHX FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MCM3AP, GANP, KIAA0572, MAP80 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), ENY2, DC6 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MCM3AP, GANP, KIAA0572, MAP80 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), ENY2, DC6 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4dhx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dhx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4dhx RCSB], [http://www.ebi.ac.uk/pdbsum/4dhx PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4dhx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dhx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4dhx RCSB], [http://www.ebi.ac.uk/pdbsum/4dhx PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/ENY2_HUMAN ENY2_HUMAN]] Involved in mRNA export coupled transcription activation by association with both the TREX-2 and the SAGA complexes. The transcription regulatory histone acetylation (HAT) complex SAGA is a multiprotein complex that activates transcription by remodeling chromatin and mediating histone acetylation and deubiquitination. Within the SAGA complex, participates in a subcomplex that specifically deubiquitinates both histones H2A and H2B. The SAGA complex is recruited to specific gene promoters by activators such as MYC, where it is required for transcription. Required for nuclear receptor-mediated transactivation. The TREX-2 complex functions in docking export-competent ribonucleoprotein particles (mRNPs) to the nuclear entrance of the nuclear pore complex (nuclear basket). TREX-2 participates in mRNA export and accurate chromatin positioning in the nucleus by tethering genes to the nuclear periphery.<ref>PMID:18206972</ref> <ref>PMID:21746879</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 19:		Line 21:
	</StructureSection>		</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
-	[[Category: Jani, D.]]	+	[[Category: Jani, D]]
-	[[Category: Stewart, M.]]	+	[[Category: Stewart, M]]
	[[Category: Mrna export]]		[[Category: Mrna export]]
	[[Category: Transport protein-dna binding protein complex]]		[[Category: Transport protein-dna binding protein complex]]

OCA at 07:52, 5 June 2014

OCA — Thu, 05 Jun 2014 07:52:18 GMT

←Older revision		Revision as of 07:52, 5 June 2014
Line 1:		Line 1:
-	[[~~Image:~~4dhx.~~jpg~~\|~~left\|200px~~]]	+	==ENY2:GANP complex==
		+	<StructureSection load='4dhx' size='340' side='right' caption='[[4dhx]], [[Resolution\|resolution]] 2.10Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4dhx]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DHX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4DHX FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MCM3AP, GANP, KIAA0572, MAP80 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), ENY2, DC6 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4dhx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dhx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4dhx RCSB], [http://www.ebi.ac.uk/pdbsum/4dhx PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Export of messenger RNA (mRNA) from the nucleus to the cytoplasm is a critical step in the gene expression pathway of eukaryotic cells. Here, we report the functional and structural characterization of the mammalian TREX-2 complex and show how it links transcription/processing with nuclear mRNA export. Mammalian TREX-2 is based on a germinal-centre associated nuclear protein (GANP) scaffold to which ENY2, PCID2 and centrins bind and depletion of any of these components inhibits mRNA export. The crystal structure of the GANP:ENY2 complex shows that two ENY2 chains interact directly with GANP, but they have different orientations from those observed on yeast Sac3. GANP is required to recruit ENY2 to nuclear pore complexes (NPCs), but ENY2 is not necessary to recruit GANP, which requires both its CID and MCM3AP domains, together with nucleoporin Nup153. GANP and ENY2 associate with RNA polymerase II and inhibition of mRNA processing redistributes GANP from NPCs into nuclear foci indicating that mammalian TREX-2 is associated with transcription. Thus, we implicate TREX-2 as an integral component of the mammalian mRNA export machinery where it links transcription and nuclear export by facilitating the transfer of mature mRNPs from the nuclear interior to NPCs.

-	<!--	+	Functional and structural characterization of the mammalian TREX-2 complex that links transcription with nuclear messenger RNA export.,Jani D, Lutz S, Hurt E, Laskey RA, Stewart M, Wickramasinghe VO Nucleic Acids Res. 2012 May 1;40(10):4562-73. Epub 2012 Feb 4. PMID:22307388<ref>PMID:22307388</ref>
-	~~The line below this paragraph~~, ~~containing "STRUCTURE_4dhx"~~, ~~creates the "Structure Box" on the page~~.	+
-	~~You may change the PDB parameter~~ (~~which sets the PDB file loaded into the applet~~)	+
-	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~	+
-	~~or leave the SCENE parameter empty for the default display~~.	+
-	-->	+
-	~~{{STRUCTURE_4dhx\| PDB=4dhx \| SCENE= }}~~	+

-	~~===ENY2:GANP complex===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-		+	== References ==
-	~~<!--~~	+	<references/>
-	~~The line below this paragraph~~, ~~{{ABSTRACT_PUBMED_22307388}}, adds~~ the ~~Publication Abstract to the page~~	+	__TOC__
-	~~(as it appears on PubMed at http://www~~.~~pubmed~~.~~gov), where 22307388 is the PubMed ID number~~.	+	</StructureSection>
-	-->	+
-	~~{{ABSTRACT_PUBMED_22307388}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[4dhx]] is a 6 chain structure with sequence from [http:~~/~~/en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DHX OCA].~~	+
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:022307388<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Jani, D.]]		[[Category: Jani, D.]]

OCA at 06:07, 13 June 2012

OCA — Wed, 13 Jun 2012 06:07:59 GMT

←Older revision		Revision as of 06:07, 13 June 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:4dhx.jpg\|left\|200px]]

-	The ~~entry 4dhx~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_4dhx", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_4dhx\| PDB=4dhx \| SCENE= }}

-	~~Authors~~: ~~Stewart, M., Jani, D.~~	+	===ENY2:GANP complex===

-	~~Description~~: ~~ENY2~~:~~GANP~~ complex	+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_22307388}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 22307388 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_22307388}}
		+
		+	==About this Structure==
		+	[[4dhx]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DHX OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:022307388</ref><references group="xtra"/>
		+	[[Category: Homo sapiens]]
		+	[[Category: Jani, D.]]
		+	[[Category: Stewart, M.]]
		+	[[Category: Mrna export]]
		+	[[Category: Transport protein-dna binding protein complex]]

OCA at 07:28, 22 February 2012

OCA — Wed, 22 Feb 2012 07:28:38 GMT

←Older revision		Revision as of 07:28, 22 February 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4dhx is ON HOLD	+	The entry 4dhx is ON HOLD until Paper Publication

	Authors: Stewart, M., Jani, D.		Authors: Stewart, M., Jani, D.

	Description: ENY2:GANP complex		Description: ENY2:GANP complex

OCA: Protected "4dhx" [edit=sysop:move=sysop]

OCA — Wed, 08 Feb 2012 06:12:43 GMT

Protected "4dhx" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:12, 8 February 2012

OCA: New page: '''Unreleased structure''' The entry 4dhx is ON HOLD Authors: Stewart, M., Jani, D. Description: ENY2:GANP complex

OCA — Wed, 08 Feb 2012 06:12:42 GMT

New page: '''Unreleased structure''' The entry 4dhx is ON HOLD Authors: Stewart, M., Jani, D. Description: ENY2:GANP complex

New page

'''Unreleased structure'''

The entry 4dhx is ON HOLD

Authors: Stewart, M., Jani, D.

Description: ENY2:GANP complex