4dol - Revision history

OCA at 14:40, 14 March 2024

OCA — Thu, 14 Mar 2024 14:40:52 GMT

←Older revision		Revision as of 14:40, 14 March 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4dol]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DOL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4DOL FirstGlance]. <br>		<table><tr><td colspan='2'>[[4dol]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DOL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4DOL FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PLM:PALMITIC+ACID'>PLM</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.7Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PLM:PALMITIC+ACID'>PLM</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4dol FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dol OCA], [https://pdbe.org/4dol PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4dol RCSB], [https://www.ebi.ac.uk/pdbsum/4dol PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4dol ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4dol FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dol OCA], [https://pdbe.org/4dol PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4dol RCSB], [https://www.ebi.ac.uk/pdbsum/4dol PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4dol ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/FAP3_ARATH FAP3_ARATH]] Fatty-acid-binding protein. Interacts with most fatty acids tested and has maximal relative affinity for C16:0.	+	[https://www.uniprot.org/uniprot/FAP3_ARATH FAP3_ARATH] Fatty-acid-binding protein. Interacts with most fatty acids tested and has maximal relative affinity for C16:0.
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	Specialized metabolic enzymes biosynthesize chemicals of ecological importance, often sharing a pedigree with primary metabolic enzymes. However, the lineage of the enzyme chalcone isomerase (CHI) remained unknown. In vascular plants, CHI-catalysed conversion of chalcones to chiral (S)-flavanones is a committed step in the production of plant flavonoids, compounds that contribute to attraction, defence and development. CHI operates near the diffusion limit with stereospecific control. Although associated primarily with plants, the CHI fold occurs in several other eukaryotic lineages and in some bacteria. Here we report crystal structures, ligand-binding properties and in vivo functional characterization of a non-catalytic CHI-fold family from plants. Arabidopsis thaliana contains five actively transcribed genes encoding CHI-fold proteins, three of which additionally encode amino-terminal chloroplast-transit sequences. These three CHI-fold proteins localize to plastids, the site of de novo fatty-acid biosynthesis in plant cells. Furthermore, their expression profiles correlate with those of core fatty-acid biosynthetic enzymes, with maximal expression occurring in seeds and coinciding with increased fatty-acid storage in the developing embryo. In vitro, these proteins are fatty-acid-binding proteins (FAPs). FAP knockout A. thaliana plants show elevated alpha-linolenic acid levels and marked reproductive defects, including aberrant seed formation. Notably, the FAP discovery defines the adaptive evolution of a stereospecific and catalytically 'perfected' enzyme from a non-enzymatic ancestor over a defined period of plant evolution.	+
-		+
-	Evolution of the chalcone-isomerase fold from fatty-acid binding to stereospecific catalysis.,Ngaki MN, Louie GV, Philippe RN, Manning G, Pojer F, Bowman ME, Li L, Larsen E, Wurtele ES, Noel JP Nature. 2012 May 13;485(7399):530-3. doi: 10.1038/nature11009. PMID:22622584<ref>PMID:22622584</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 4dol" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references/>~~	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 08:32, 21 September 2022

OCA — Wed, 21 Sep 2022 08:32:08 GMT

←Older revision		Revision as of 08:32, 21 September 2022
Line 1:		Line 1:

	==Crystal structure of Arabidopsis thaliana fatty-acid binding protein At1g53520 (AtFAP3)==		==Crystal structure of Arabidopsis thaliana fatty-acid binding protein At1g53520 (AtFAP3)==
-	<StructureSection load='4dol' size='340' side='right' caption='[[4dol]], [[Resolution\|resolution]] 1.70Å' scene=''>	+	<StructureSection load='4dol' size='340' side='right'caption='[[4dol]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4dol]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Arath Arath~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DOL OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4DOL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4dol]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DOL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4DOL FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PLM:PALMITIC+ACID'>PLM</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PLM:PALMITIC+ACID'>PLM</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4doi\|4doi]], [[4dok\|4dok]], [[4doo\|4doo]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4dol FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dol OCA], [https://pdbe.org/4dol PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4dol RCSB], [https://www.ebi.ac.uk/pdbsum/4dol PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4dol ProSAT]</span></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">At1g53520, F22G10.11 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])~~</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4dol FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dol OCA], [~~http~~://pdbe.org/4dol PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4dol RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4dol PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4dol ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/FAP3_ARATH FAP3_ARATH]] Fatty-acid-binding protein. Interacts with most fatty acids tested and has maximal relative affinity for C16:0.	+	[[https://www.uniprot.org/uniprot/FAP3_ARATH FAP3_ARATH]] Fatty-acid-binding protein. Interacts with most fatty acids tested and has maximal relative affinity for C16:0.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Arath~~]]	+	[[Category: Arabidopsis thaliana]]
-	[[Category: ~~Bowman, M E~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Louie, G V~~]]	+	[[Category: Bowman ME]]
-	[[Category: ~~Noel, J P~~]]	+	[[Category: Louie GV]]
-	[[Category: ~~Chalcone-isomerase like fold]]~~	+	[[Category: Noel JP]]
-	~~[[Category: Fatty-acid binding]]~~	+
-	~~[[Category: Isomerase~~]]	+

OCA at 14:04, 5 August 2016

OCA — Fri, 05 Aug 2016 14:04:06 GMT

←Older revision		Revision as of 14:04, 5 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of Arabidopsis thaliana fatty-acid binding protein At1g53520 (AtFAP3)==		==Crystal structure of Arabidopsis thaliana fatty-acid binding protein At1g53520 (AtFAP3)==
	<StructureSection load='4dol' size='340' side='right' caption='[[4dol]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='4dol' size='340' side='right' caption='[[4dol]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4dol]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Arabidopsis_thaliana Arabidopsis thaliana~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DOL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4DOL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4dol]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DOL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4DOL FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PLM:PALMITIC+ACID'>PLM</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PLM:PALMITIC+ACID'>PLM</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4doi\|4doi]], [[4dok\|4dok]], [[4doo\|4doo]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4doi\|4doi]], [[4dok\|4dok]], [[4doo\|4doo]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">At1g53520, F22G10.11 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ~~Arabidopsis thaliana~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">At1g53520, F22G10.11 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4dol FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dol OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4dol RCSB], [http://www.ebi.ac.uk/pdbsum/4dol PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4dol FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dol OCA], [http://pdbe.org/4dol PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4dol RCSB], [http://www.ebi.ac.uk/pdbsum/4dol PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4dol ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[http://www.uniprot.org/uniprot/~~Q9C8L2_ARATH Q9C8L2_ARATH~~]] Fatty-acid-binding protein. Interacts with most fatty acids tested and has maximal relative affinity for C16:0.	+	[[http://www.uniprot.org/uniprot/FAP3_ARATH FAP3_ARATH]] Fatty-acid-binding protein. Interacts with most fatty acids tested and has maximal relative affinity for C16:0.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4dol" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Arabidopsis thaliana~~]]	+	[[Category: Arath]]
	[[Category: Bowman, M E]]		[[Category: Bowman, M E]]
	[[Category: Louie, G V]]		[[Category: Louie, G V]]

OCA at 03:06, 25 December 2014

OCA — Thu, 25 Dec 2014 03:06:25 GMT

←Older revision		Revision as of 03:06, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4dol]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DOL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4DOL FirstGlance]. <br>		<table><tr><td colspan='2'>[[4dol]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DOL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4DOL FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PLM:PALMITIC+ACID'>PLM</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PLM:PALMITIC+ACID'>PLM</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4doi\|4doi]], [[4dok\|4dok]], [[4doo\|4doo]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4doi\|4doi]], [[4dok\|4dok]], [[4doo\|4doo]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">At1g53520, F22G10.11 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 Arabidopsis thaliana])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">At1g53520, F22G10.11 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 Arabidopsis thaliana])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4dol FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dol OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4dol RCSB], [http://www.ebi.ac.uk/pdbsum/4dol PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4dol FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dol OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4dol RCSB], [http://www.ebi.ac.uk/pdbsum/4dol PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/Q9C8L2_ARATH Q9C8L2_ARATH]] Fatty-acid-binding protein. Interacts with most fatty acids tested and has maximal relative affinity for C16:0.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 23:
	</StructureSection>		</StructureSection>
	[[Category: Arabidopsis thaliana]]		[[Category: Arabidopsis thaliana]]
-	[[Category: Bowman, M E.]]	+	[[Category: Bowman, M E]]
-	[[Category: Louie, G V.]]	+	[[Category: Louie, G V]]
-	[[Category: Noel, J P.]]	+	[[Category: Noel, J P]]
	[[Category: Chalcone-isomerase like fold]]		[[Category: Chalcone-isomerase like fold]]
	[[Category: Fatty-acid binding]]		[[Category: Fatty-acid binding]]
	[[Category: Isomerase]]		[[Category: Isomerase]]

OCA at 08:26, 5 June 2014

OCA — Thu, 05 Jun 2014 08:26:33 GMT

←Older revision		Revision as of 08:26, 5 June 2014
Line 1:		Line 1:
-	[[~~Image:~~4dol.~~png~~\|~~left~~\|~~200px~~]]	+	==Crystal structure of Arabidopsis thaliana fatty-acid binding protein At1g53520 (AtFAP3)==
		+	<StructureSection load='4dol' size='340' side='right' caption='[[4dol]], [[Resolution\|resolution]] 1.70Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4dol]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DOL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4DOL FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PLM:PALMITIC+ACID'>PLM</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4doi\|4doi]], [[4dok\|4dok]], [[4doo\|4doo]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">At1g53520, F22G10.11 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 Arabidopsis thaliana])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4dol FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dol OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4dol RCSB], [http://www.ebi.ac.uk/pdbsum/4dol PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Specialized metabolic enzymes biosynthesize chemicals of ecological importance, often sharing a pedigree with primary metabolic enzymes. However, the lineage of the enzyme chalcone isomerase (CHI) remained unknown. In vascular plants, CHI-catalysed conversion of chalcones to chiral (S)-flavanones is a committed step in the production of plant flavonoids, compounds that contribute to attraction, defence and development. CHI operates near the diffusion limit with stereospecific control. Although associated primarily with plants, the CHI fold occurs in several other eukaryotic lineages and in some bacteria. Here we report crystal structures, ligand-binding properties and in vivo functional characterization of a non-catalytic CHI-fold family from plants. Arabidopsis thaliana contains five actively transcribed genes encoding CHI-fold proteins, three of which additionally encode amino-terminal chloroplast-transit sequences. These three CHI-fold proteins localize to plastids, the site of de novo fatty-acid biosynthesis in plant cells. Furthermore, their expression profiles correlate with those of core fatty-acid biosynthetic enzymes, with maximal expression occurring in seeds and coinciding with increased fatty-acid storage in the developing embryo. In vitro, these proteins are fatty-acid-binding proteins (FAPs). FAP knockout A. thaliana plants show elevated alpha-linolenic acid levels and marked reproductive defects, including aberrant seed formation. Notably, the FAP discovery defines the adaptive evolution of a stereospecific and catalytically 'perfected' enzyme from a non-enzymatic ancestor over a defined period of plant evolution.

-	<!--	+	Evolution of the chalcone-isomerase fold from fatty-acid binding to stereospecific catalysis.,Ngaki MN, Louie GV, Philippe RN, Manning G, Pojer F, Bowman ME, Li L, Larsen E, Wurtele ES, Noel JP Nature. 2012 May 13;485(7399):530-3. doi: 10.1038/nature11009. PMID:22622584<ref>PMID:22622584</ref>
-	~~The line below this paragraph~~, ~~containing "STRUCTURE_4dol"~~, ~~creates the "Structure Box" on the page~~.	+
-	~~You may change the PDB parameter~~ (~~which sets the PDB file loaded into the applet~~)	+
-	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~	+
-	~~or leave the SCENE parameter empty for the default display.~~	+
-	-->	+
-	~~{{STRUCTURE_4dol\| PDB=4dol \| SCENE= }}~~	+

-	~~===Crystal structure~~ of ~~Arabidopsis thaliana fatty-acid binding protein At1g53520 (AtFAP3)===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-		+	== References ==
-	~~<!--~~	+	<references/>
-	~~The line below this paragraph, {{ABSTRACT_PUBMED_22622584}}, adds~~ the ~~Publication Abstract to the page~~	+	__TOC__
-	~~(as it appears on PubMed at http://www~~.~~pubmed~~.~~gov), where 22622584 is the PubMed ID number~~.	+	</StructureSection>
-	-->	+
-	~~{{ABSTRACT_PUBMED_22622584}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[4dol]] is a 1 chain structure with sequence from [http:~~/~~/en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DOL OCA].~~	+
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:022622584<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Arabidopsis thaliana]]		[[Category: Arabidopsis thaliana]]
	[[Category: Bowman, M E.]]		[[Category: Bowman, M E.]]

OCA at 06:46, 6 June 2012

OCA — Wed, 06 Jun 2012 06:46:33 GMT

←Older revision		Revision as of 06:46, 6 June 2012
Line 1:		Line 1:
-	[[Image:4dol.~~jpg~~\|left\|200px]]	+	[[Image:4dol.png\|left\|200px]]

	<!--		<!--
Line 11:		Line 11:
	===Crystal structure of Arabidopsis thaliana fatty-acid binding protein At1g53520 (AtFAP3)===		===Crystal structure of Arabidopsis thaliana fatty-acid binding protein At1g53520 (AtFAP3)===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_22622584}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 22622584 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_22622584}}

	==About this Structure==		==About this Structure==
	[[4dol]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DOL OCA].		[[4dol]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DOL OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:022622584</ref><references group="xtra"/>
	[[Category: Arabidopsis thaliana]]		[[Category: Arabidopsis thaliana]]
	[[Category: Bowman, M E.]]		[[Category: Bowman, M E.]]

OCA at 09:26, 9 May 2012

OCA — Wed, 09 May 2012 09:26:15 GMT

←Older revision		Revision as of 09:26, 9 May 2012
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-	~~'''Unreleased structure'''~~	+	[[Image:4dol.jpg\|left\|200px]]

-	The ~~entry 4dol~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_4dol", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_4dol\| PDB=4dol \| SCENE= }}

-	~~Authors: Noel, J.P., Louie, G.V., Bowman, M.E.~~	+	===Crystal structure of Arabidopsis thaliana fatty-acid binding protein At1g53520 (AtFAP3)===

-	~~Description: Crystal~~ structure of Arabidopsis thaliana ~~fatty~~-acid binding ~~protein At1g53520 (AtFAP3)~~	+
		+	==About this Structure==
		+	[[4dol]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DOL OCA].
		+	[[Category: Arabidopsis thaliana]]
		+	[[Category: Bowman, M E.]]
		+	[[Category: Louie, G V.]]
		+	[[Category: Noel, J P.]]
		+	[[Category: Chalcone-isomerase like fold]]
		+	[[Category: Fatty-acid binding]]
		+	[[Category: Isomerase]]

OCA at 06:11, 14 March 2012

OCA — Wed, 14 Mar 2012 06:11:17 GMT

←Older revision		Revision as of 06:11, 14 March 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4dol is ON HOLD	+	The entry 4dol is ON HOLD until Paper Publication

	Authors: Noel, J.P., Louie, G.V., Bowman, M.E.		Authors: Noel, J.P., Louie, G.V., Bowman, M.E.

	Description: Crystal structure of Arabidopsis thaliana fatty-acid binding protein At1g53520 (AtFAP3)		Description: Crystal structure of Arabidopsis thaliana fatty-acid binding protein At1g53520 (AtFAP3)

OCA at 07:31, 22 February 2012

OCA — Wed, 22 Feb 2012 07:31:01 GMT

←Older revision		Revision as of 07:31, 22 February 2012
Line 3:		Line 3:
	The entry 4dol is ON HOLD		The entry 4dol is ON HOLD

-	Authors: Noel, ~~Joseph~~ P., Louie, ~~Gordon~~ V., Bowman, ~~Marianne~~ E.	+	Authors: Noel, J.P., Louie, G.V., Bowman, M.E.

	Description: Crystal structure of Arabidopsis thaliana fatty-acid binding protein At1g53520 (AtFAP3)		Description: Crystal structure of Arabidopsis thaliana fatty-acid binding protein At1g53520 (AtFAP3)

OCA: Protected "4dol" [edit=sysop:move=sysop]

OCA — Wed, 15 Feb 2012 06:26:51 GMT

Protected "4dol" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:26, 15 February 2012