4dqj - Revision history

OCA at 10:54, 1 March 2024

OCA — Fri, 01 Mar 2024 10:54:40 GMT

←Older revision		Revision as of 10:54, 1 March 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4dqj]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_virus_phi6 Pseudomonas virus phi6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DQJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4DQJ FirstGlance]. <br>		<table><tr><td colspan='2'>[[4dqj]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_virus_phi6 Pseudomonas virus phi6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DQJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4DQJ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AES:4-(2-AMINOETHYL)BENZENESULFONYL+FLUORIDE'>AES</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.23Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AES:4-(2-AMINOETHYL)BENZENESULFONYL+FLUORIDE'>AES</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4dqj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dqj OCA], [https://pdbe.org/4dqj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4dqj RCSB], [https://www.ebi.ac.uk/pdbsum/4dqj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4dqj ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4dqj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dqj OCA], [https://pdbe.org/4dqj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4dqj RCSB], [https://www.ebi.ac.uk/pdbsum/4dqj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4dqj ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/Q283U5_BPPH6 Q283U5_BPPH6]]	+	[https://www.uniprot.org/uniprot/Q283U5_BPPH6 Q283U5_BPPH6]
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	The modulation of fitness by single mutational substitutions during environmental change is the most fundamental consequence of natural selection. The antagonistic tradeoffs of pleiotropic mutations that can be selected under changing environments therefore lie at the foundation of evolutionary biology. However, the molecular basis of fitness tradeoffs is rarely determined in terms of how these pleiotropic mutations affect protein structure. Here we use an interdisciplinary approach to study how antagonistic pleiotropy and protein function dictate a fitness tradeoff. We challenged populations of an RNA virus, bacteriophage Phi6, to evolve in a novel temperature environment where heat shock imposed extreme virus mortality. A single amino acid substitution in the viral lysin protein P5 (V207F) favored improved stability, and hence survival of challenged viruses, despite a concomitant tradeoff that decreased viral reproduction. This mutation increased the thermostability of P5. Crystal structures of wild-type, mutant, and ligand-bound P5 reveal the molecular basis of this thermostabilization--the Phe207 side chain fills a hydrophobic cavity that is unoccupied in the wild-type--and identify P5 as a lytic transglycosylase. The mutation did not reduce the enzymatic activity of P5, suggesting that the reproduction tradeoff stems from other factors such as inefficient capsid assembly or disassembly. Our study demonstrates how combining experimental evolution, biochemistry, and structural biology can identify the mechanisms that drive the antagonistic pleiotropic phenotypes of an individual point mutation in the classic evolutionary tug-of-war between survival and reproduction.	+

-	Selective pressure causes an RNA virus to trade reproductive fitness for increased structural and thermal stability of a viral enzyme.,Dessau M, Goldhill D, McBride R, Turner PE, Modis Y PLoS Genet. 2012;8(11):e1003102. doi: 10.1371/journal.pgen.1003102. Epub 2012 Nov, 29. PMID:23209446<ref>PMID:23209446</ref>	+	==See Also==
-		+	*[[Lysin 3D structures\|Lysin 3D structures]]
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 4dqj" style="background-color:#fffaf0;"></div>~~	+
-	== ~~References~~ ==	+
-	~~<references/>~~	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 08:34, 21 September 2022

OCA — Wed, 21 Sep 2022 08:34:30 GMT

←Older revision		Revision as of 08:34, 21 September 2022
Line 3:		Line 3:
	<StructureSection load='4dqj' size='340' side='right'caption='[[4dqj]], [[Resolution\|resolution]] 1.23Å' scene=''>		<StructureSection load='4dqj' size='340' side='right'caption='[[4dqj]], [[Resolution\|resolution]] 1.23Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4dqj]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Bpph6 Bpph6~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DQJ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4DQJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4dqj]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_virus_phi6 Pseudomonas virus phi6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DQJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4DQJ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AES:4-(2-AMINOETHYL)BENZENESULFONYL+FLUORIDE'>AES</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AES:4-(2-AMINOETHYL)BENZENESULFONYL+FLUORIDE'>AES</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4dq5\|4dq5]], [[4dq7\|4dq7]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4dqj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dqj OCA], [https://pdbe.org/4dqj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4dqj RCSB], [https://www.ebi.ac.uk/pdbsum/4dqj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4dqj ProSAT]</span></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">P5, p5a ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10879 BPPH6])~~</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4dqj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dqj OCA], [~~http~~://pdbe.org/4dqj PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4dqj RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4dqj PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4dqj ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/Q283U5_BPPH6 Q283U5_BPPH6]]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Bpph6]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Dessau, M A~~]]	+	[[Category: Pseudomonas virus phi6]]
-	[[Category: ~~Modis, Y~~]]	+	[[Category: Dessau MA]]
-	[[Category: ~~Hydrolase]]~~	+	[[Category: Modis Y]]
-	~~[[Category: Lysin]]~~	+
-	~~[[Category: Thermotolerance]]~~	+
-	~~[[Category: Transglycosylase~~]]	+

OCA at 08:06, 27 March 2019

OCA — Wed, 27 Mar 2019 08:06:25 GMT

←Older revision		Revision as of 08:06, 27 March 2019
Line 1:		Line 1:

	==Structural Investigation of Bacteriophage Phi6 Lysin (in complex with chitotetraose)==		==Structural Investigation of Bacteriophage Phi6 Lysin (in complex with chitotetraose)==
-	<StructureSection load='4dqj' size='340' side='right' caption='[[4dqj]], [[Resolution\|resolution]] 1.23Å' scene=''>	+	<StructureSection load='4dqj' size='340' side='right'caption='[[4dqj]], [[Resolution\|resolution]] 1.23Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4dqj]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DQJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4DQJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4dqj]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpph6 Bpph6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DQJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4DQJ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AES:4-(2-AMINOETHYL)BENZENESULFONYL+FLUORIDE'>AES</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AES:4-(2-AMINOETHYL)BENZENESULFONYL+FLUORIDE'>AES</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4dq5\|4dq5]], [[4dq7\|4dq7]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4dq5\|4dq5]], [[4dq7\|4dq7]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">P5, p5a ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10879 BPPH6])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4dqj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dqj OCA], [http://pdbe.org/4dqj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4dqj RCSB], [http://www.ebi.ac.uk/pdbsum/4dqj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4dqj ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4dqj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dqj OCA], [http://pdbe.org/4dqj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4dqj RCSB], [http://www.ebi.ac.uk/pdbsum/4dqj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4dqj ProSAT]</span></td></tr>
	</table>		</table>
Line 21:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bpph6]]
		+	[[Category: Large Structures]]
	[[Category: Dessau, M A]]		[[Category: Dessau, M A]]
	[[Category: Modis, Y]]		[[Category: Modis, Y]]

OCA at 11:03, 1 February 2017

OCA — Wed, 01 Feb 2017 11:03:22 GMT

←Older revision		Revision as of 11:03, 1 February 2017
Line 3:		Line 3:
	<StructureSection load='4dqj' size='340' side='right' caption='[[4dqj]], [[Resolution\|resolution]] 1.23Å' scene=''>		<StructureSection load='4dqj' size='340' side='right' caption='[[4dqj]], [[Resolution\|resolution]] 1.23Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4dqj]] is a 2 chain structure ~~with sequence from [http://en.wikipedia.org/wiki/Bpph6 Bpph6]~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DQJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4DQJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4dqj]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DQJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4DQJ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AES:4-(2-AMINOETHYL)BENZENESULFONYL+FLUORIDE'>AES</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AES:4-(2-AMINOETHYL)BENZENESULFONYL+FLUORIDE'>AES</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4dq5\|4dq5]], [[4dq7\|4dq7]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4dq5\|4dq5]], [[4dq7\|4dq7]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">P5, p5a ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10879 BPPH6])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4dqj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dqj OCA], [http://pdbe.org/4dqj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4dqj RCSB], [http://www.ebi.ac.uk/pdbsum/4dqj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4dqj ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4dqj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dqj OCA], [http://pdbe.org/4dqj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4dqj RCSB], [http://www.ebi.ac.uk/pdbsum/4dqj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4dqj ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The modulation of fitness by single mutational substitutions during environmental change is the most fundamental consequence of natural selection. The antagonistic tradeoffs of pleiotropic mutations that can be selected under changing environments therefore lie at the foundation of evolutionary biology. However, the molecular basis of fitness tradeoffs is rarely determined in terms of how these pleiotropic mutations affect protein structure. Here we use an interdisciplinary approach to study how antagonistic pleiotropy and protein function dictate a fitness tradeoff. We challenged populations of an RNA virus, bacteriophage Phi6, to evolve in a novel temperature environment where heat shock imposed extreme virus mortality. A single amino acid substitution in the viral lysin protein P5 (V207F) favored improved stability, and hence survival of challenged viruses, despite a concomitant tradeoff that decreased viral reproduction. This mutation increased the thermostability of P5. Crystal structures of wild-type, mutant, and ligand-bound P5 reveal the molecular basis of this thermostabilization--the Phe207 side chain fills a hydrophobic cavity that is unoccupied in the wild-type--and identify P5 as a lytic transglycosylase. The mutation did not reduce the enzymatic activity of P5, suggesting that the reproduction tradeoff stems from other factors such as inefficient capsid assembly or disassembly. Our study demonstrates how combining experimental evolution, biochemistry, and structural biology can identify the mechanisms that drive the antagonistic pleiotropic phenotypes of an individual point mutation in the classic evolutionary tug-of-war between survival and reproduction.
		+
		+	Selective pressure causes an RNA virus to trade reproductive fitness for increased structural and thermal stability of a viral enzyme.,Dessau M, Goldhill D, McBride R, Turner PE, Modis Y PLoS Genet. 2012;8(11):e1003102. doi: 10.1371/journal.pgen.1003102. Epub 2012 Nov, 29. PMID:23209446<ref>PMID:23209446</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 4dqj" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Bpph6]]
	[[Category: Dessau, M A]]		[[Category: Dessau, M A]]
	[[Category: Modis, Y]]		[[Category: Modis, Y]]

OCA at 02:45, 11 December 2016

OCA — Sun, 11 Dec 2016 02:45:55 GMT

←Older revision		Revision as of 02:45, 11 December 2016
Line 1:		Line 1:
		+
	==Structural Investigation of Bacteriophage Phi6 Lysin (in complex with chitotetraose)==		==Structural Investigation of Bacteriophage Phi6 Lysin (in complex with chitotetraose)==
	<StructureSection load='4dqj' size='340' side='right' caption='[[4dqj]], [[Resolution\|resolution]] 1.23Å' scene=''>		<StructureSection load='4dqj' size='340' side='right' caption='[[4dqj]], [[Resolution\|resolution]] 1.23Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4dqj]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Pseudomonas_phage_phi6 Pseudomonas phage phi6~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DQJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4DQJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4dqj]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpph6 Bpph6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DQJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4DQJ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AES:4-(2-AMINOETHYL)BENZENESULFONYL+FLUORIDE'>AES</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AES:4-(2-AMINOETHYL)BENZENESULFONYL+FLUORIDE'>AES</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4dq5\|4dq5]], [[4dq7\|4dq7]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4dq5\|4dq5]], [[4dq7\|4dq7]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">P5, p5a ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10879 ~~Pseudomonas phage phi6~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">P5, p5a ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10879 BPPH6])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4dqj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dqj OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4dqj RCSB], [http://www.ebi.ac.uk/pdbsum/4dqj PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4dqj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dqj OCA], [http://pdbe.org/4dqj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4dqj RCSB], [http://www.ebi.ac.uk/pdbsum/4dqj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4dqj ProSAT]</span></td></tr>
	</table>		</table>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Pseudomonas phage phi6~~]]	+	[[Category: Bpph6]]
	[[Category: Dessau, M A]]		[[Category: Dessau, M A]]
	[[Category: Modis, Y]]		[[Category: Modis, Y]]

OCA at 15:54, 9 December 2014

OCA — Tue, 09 Dec 2014 15:54:53 GMT

←Older revision		Revision as of 15:54, 9 December 2014
Line 1:		Line 1:
-	~~[[Image:4dqj.jpg\|left\|200px]]~~	+	==Structural Investigation of Bacteriophage Phi6 Lysin (in complex with chitotetraose)==
-		+	<StructureSection load='4dqj' size='340' side='right' caption='[[4dqj]], [[Resolution\|resolution]] 1.23Å' scene=''>
-	~~{{STRUCTURE_4dqj\| PDB=4dqj \| SCENE= }}~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[4dqj]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_phage_phi6 Pseudomonas phage phi6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DQJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4DQJ FirstGlance]. <br>
-	===Structural Investigation of Bacteriophage Phi6 Lysin (in complex with chitotetraose)===	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AES:4-(2-AMINOETHYL)BENZENESULFONYL+FLUORIDE'>AES</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4dq5\|4dq5]], [[4dq7\|4dq7]]</td></tr>
-		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">P5, p5a ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10879 Pseudomonas phage phi6])</td></tr>
-	==~~About this Structure~~==	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4dqj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dqj OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4dqj RCSB], [http://www.ebi.ac.uk/pdbsum/4dqj PDBsum]</span></td></tr>
-	[[4dqj]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_phage_phi6 Pseudomonas phage phi6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DQJ OCA].	+	</table>
		+	__TOC__
		+	</StructureSection>
	[[Category: Pseudomonas phage phi6]]		[[Category: Pseudomonas phage phi6]]
-	[[Category: Dessau, M A.]]	+	[[Category: Dessau, M A]]
-	[[Category: Modis, Y.]]	+	[[Category: Modis, Y]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Lysin]]		[[Category: Lysin]]
	[[Category: Thermotolerance]]		[[Category: Thermotolerance]]
	[[Category: Transglycosylase]]		[[Category: Transglycosylase]]

OCA at 09:35, 2 January 2013

OCA — Wed, 02 Jan 2013 09:35:11 GMT

←Older revision		Revision as of 09:35, 2 January 2013
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:4dqj.jpg\|left\|200px]]

-	~~The entry~~ 4dqj ~~is ON HOLD~~ ~~until Paper Publication~~	+	{{STRUCTURE_4dqj\| PDB=4dqj \| SCENE= }}

-	~~Authors: Dessau, M.A., Modis, Y.~~	+	===Structural Investigation of Bacteriophage Phi6 Lysin (in complex with chitotetraose)===

-	~~Description~~: ~~Structural Investigation of Bacteriophage Phi6~~ Lysin	+
		+	==About this Structure==
		+	[[4dqj]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_phage_phi6 Pseudomonas phage phi6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DQJ OCA].
		+	[[Category: Pseudomonas phage phi6]]
		+	[[Category: Dessau, M A.]]
		+	[[Category: Modis, Y.]]
		+	[[Category: Hydrolase]]
		+	[[Category: Lysin]]
		+	[[Category: Thermotolerance]]
		+	[[Category: Transglycosylase]]

OCA at 08:44, 16 May 2012

OCA — Wed, 16 May 2012 08:44:42 GMT

←Older revision		Revision as of 08:44, 16 May 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4dqj is ON HOLD	+	The entry 4dqj is ON HOLD until Paper Publication

	Authors: Dessau, M.A., Modis, Y.		Authors: Dessau, M.A., Modis, Y.

	Description: Structural Investigation of Bacteriophage Phi6 Lysin		Description: Structural Investigation of Bacteriophage Phi6 Lysin

OCA at 07:19, 28 March 2012

OCA — Wed, 28 Mar 2012 07:19:38 GMT

←Older revision		Revision as of 07:19, 28 March 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4dqj is ON HOLD ~~until Paper Publication~~	+	The entry 4dqj is ON HOLD

	Authors: Dessau, M.A., Modis, Y.		Authors: Dessau, M.A., Modis, Y.

	Description: Structural Investigation of Bacteriophage Phi6 Lysin		Description: Structural Investigation of Bacteriophage Phi6 Lysin

OCA at 07:12, 21 March 2012

OCA — Wed, 21 Mar 2012 07:12:10 GMT

←Older revision		Revision as of 07:12, 21 March 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4dqj is ON HOLD	+	The entry 4dqj is ON HOLD until Paper Publication

	Authors: Dessau, M.A., Modis, Y.		Authors: Dessau, M.A., Modis, Y.

	Description: Structural Investigation of Bacteriophage Phi6 Lysin		Description: Structural Investigation of Bacteriophage Phi6 Lysin