4ekv - Revision history

OCA at 15:03, 14 March 2024

OCA — Thu, 14 Mar 2024 15:03:53 GMT

←Older revision		Revision as of 15:03, 14 March 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4ekv]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EKV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EKV FirstGlance]. <br>		<table><tr><td colspan='2'>[[4ekv]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EKV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EKV FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BTN:BIOTIN'>BTN</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BTN:BIOTIN'>BTN</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ekv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ekv OCA], [https://pdbe.org/4ekv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ekv RCSB], [https://www.ebi.ac.uk/pdbsum/4ekv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ekv ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ekv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ekv OCA], [https://pdbe.org/4ekv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ekv RCSB], [https://www.ebi.ac.uk/pdbsum/4ekv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ekv ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV]] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).	+	[https://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	A novel form of tetrameric streptavidin has been engineered to have reversible biotin binding capability. In wild-type streptavidin, loop(3-4) functions as a lid for the entry and exit of biotin. When biotin is bound, interactions between biotin and key residues in loop(3-4) keep this lid in the closed state. In the engineered mutein, a second biotin exit door is created by changing the amino acid sequence of loop(7-8). This door is mobile even in the presence of the bound biotin and can facilitate the release of biotin from the mutein. Since loop(7-8) is involved in subunit interactions, alteration of this loop in the engineered mutein results in an 11 degrees rotation between the two dimers in reference to wild-type streptavidin. The tetrameric state of the engineered mutein is stabilized by a H127C mutation, which leads to the formation of inter-subunit disulfide bonds. The biotin binding kinetic parameters (k(off) of 4.28x10(-4) s(-1) and K(d) of 1.9x10(-8) M) make this engineered mutein a superb affinity agent for the purification of biotinylated biomolecules. Affinity matrices can be regenerated using gentle procedures, and regenerated matrices can be reused at least ten times without any observable reduction in binding capacity. With the combination of both the engineered mutein and wild-type streptavidin, biotinylated biomolecules can easily be affinity purified to high purity and immobilized to desirable platforms without any leakage concerns. Other potential biotechnological applications, such as development of an automated high-throughput protein purification system, are feasible.	+
-		+
-	Development of a tetrameric streptavidin mutein with reversible biotin binding capability: engineering a mobile loop as an exit door for biotin.,O'Sullivan VJ, Barrette-Ng I, Hommema E, Hermanson GT, Schofield M, Wu SC, Honetschlaeger C, Ng KK, Wong SL PLoS One. 2012;7(4):e35203. Epub 2012 Apr 19. PMID:22536357<ref>PMID:22536357</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 4ekv" style="background-color:#fffaf0;"></div>~~	+

	==See Also==		==See Also==
	*[[Avidin 3D structures\|Avidin 3D structures]]		*[[Avidin 3D structures\|Avidin 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 07:15, 28 September 2022

OCA — Wed, 28 Sep 2022 07:15:31 GMT

←Older revision		Revision as of 07:15, 28 September 2022
Line 3:		Line 3:
	<StructureSection load='4ekv' size='340' side='right'caption='[[4ekv]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='4ekv' size='340' side='right'caption='[[4ekv]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4ekv]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~As_4.1583 As 4.1583~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EKV OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4EKV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4ekv]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EKV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EKV FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BTN:BIOTIN'>BTN</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BTN:BIOTIN'>BTN</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1swe\|1swe]]~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ekv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ekv OCA], [https://pdbe.org/4ekv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ekv RCSB], [https://www.ebi.ac.uk/pdbsum/4ekv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ekv ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4ekv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ekv OCA], [~~http~~://pdbe.org/4ekv PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4ekv RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4ekv PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4ekv ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV]] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).	+	[[https://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV]] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 26:		Line 25:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: As 4 1583]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Barrette-Ng, I H]]~~	+	[[Category: Streptomyces avidinii]]
-	~~[[Category: Honetschlaeger, C~~]]	+	[[Category: Barrette-Ng IH]]
-	[[Category: Ng~~, K K.S~~]]	+	[[Category: Honetschlaeger C]]
-	[[Category: ~~Wong, S L~~]]	+	[[Category: Ng KKS]]
-	[[Category: ~~Beta-barrel~~]]	+	[[Category: Wong SL]]
-	[[Category: ~~Binding protein]]~~	+
-	~~[[Category: Biotin-binding]]~~	+
-	~~[[Category: Biotin-binding protein~~]]	+

OCA at 09:21, 5 February 2020

OCA — Wed, 05 Feb 2020 09:21:52 GMT

←Older revision		Revision as of 09:21, 5 February 2020
Line 1:		Line 1:

	==Streptavidin 8-aa-loop H127C mutein with reversible biotin binding==		==Streptavidin 8-aa-loop H127C mutein with reversible biotin binding==
-	<StructureSection load='4ekv' size='340' side='right' caption='[[4ekv]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='4ekv' size='340' side='right'caption='[[4ekv]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4ekv]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/As_4.1583 As 4.1583]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EKV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EKV FirstGlance]. <br>		<table><tr><td colspan='2'>[[4ekv]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/As_4.1583 As 4.1583]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EKV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EKV FirstGlance]. <br>
Line 19:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 4ekv" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4ekv" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Avidin 3D structures\|Avidin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 24:		Line 27:
	</StructureSection>		</StructureSection>
	[[Category: As 4 1583]]		[[Category: As 4 1583]]
		+	[[Category: Large Structures]]
	[[Category: Barrette-Ng, I H]]		[[Category: Barrette-Ng, I H]]
	[[Category: Honetschlaeger, C]]		[[Category: Honetschlaeger, C]]

OCA at 08:05, 15 November 2017

OCA — Wed, 15 Nov 2017 08:05:31 GMT

←Older revision		Revision as of 08:05, 15 November 2017
Line 19:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 4ekv" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4ekv" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Avidin\|Avidin]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 21:18, 4 August 2016

OCA — Thu, 04 Aug 2016 21:18:53 GMT

←Older revision		Revision as of 21:18, 4 August 2016
Line 1:		Line 1:
		+
	==Streptavidin 8-aa-loop H127C mutein with reversible biotin binding==		==Streptavidin 8-aa-loop H127C mutein with reversible biotin binding==
	<StructureSection load='4ekv' size='340' side='right' caption='[[4ekv]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='4ekv' size='340' side='right' caption='[[4ekv]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4ekv]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Streptomyces_avidinii Streptomyces avidinii~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EKV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EKV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4ekv]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/As_4.1583 As 4.1583]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EKV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EKV FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BTN:BIOTIN'>BTN</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BTN:BIOTIN'>BTN</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1swe\|1swe]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1swe\|1swe]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ekv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ekv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ekv RCSB], [http://www.ebi.ac.uk/pdbsum/4ekv PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ekv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ekv OCA], [http://pdbe.org/4ekv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ekv RCSB], [http://www.ebi.ac.uk/pdbsum/4ekv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ekv ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4ekv" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 24:		Line 26:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Streptomyces avidinii~~]]	+	[[Category: As 4 1583]]
	[[Category: Barrette-Ng, I H]]		[[Category: Barrette-Ng, I H]]
	[[Category: Honetschlaeger, C]]		[[Category: Honetschlaeger, C]]

OCA at 19:44, 24 December 2014

OCA — Wed, 24 Dec 2014 19:44:56 GMT

←Older revision		Revision as of 19:44, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4ekv]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EKV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EKV FirstGlance]. <br>		<table><tr><td colspan='2'>[[4ekv]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EKV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EKV FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BTN:BIOTIN'>BTN</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BTN:BIOTIN'>BTN</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1swe\|1swe]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1swe\|1swe]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ekv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ekv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ekv RCSB], [http://www.ebi.ac.uk/pdbsum/4ekv PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ekv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ekv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ekv RCSB], [http://www.ebi.ac.uk/pdbsum/4ekv PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV]] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 25:
	</StructureSection>		</StructureSection>
	[[Category: Streptomyces avidinii]]		[[Category: Streptomyces avidinii]]
-	[[Category: Barrette-Ng, I H.]]	+	[[Category: Barrette-Ng, I H]]
-	[[Category: Honetschlaeger, C.]]	+	[[Category: Honetschlaeger, C]]
-	[[Category: Ng, K K.S.]]	+	[[Category: Ng, K K.S]]
-	[[Category: Wong, S L.]]	+	[[Category: Wong, S L]]
	[[Category: Beta-barrel]]		[[Category: Beta-barrel]]
	[[Category: Binding protein]]		[[Category: Binding protein]]
	[[Category: Biotin-binding]]		[[Category: Biotin-binding]]
	[[Category: Biotin-binding protein]]		[[Category: Biotin-binding protein]]

OCA at 07:30, 5 June 2014

OCA — Thu, 05 Jun 2014 07:30:38 GMT

←Older revision		Revision as of 07:30, 5 June 2014
Line 1:		Line 1:
-	[[~~Image:~~4ekv.~~jpg~~\|~~left~~\|~~200px~~]]	+	==Streptavidin 8-aa-loop H127C mutein with reversible biotin binding==
		+	<StructureSection load='4ekv' size='340' side='right' caption='[[4ekv]], [[Resolution\|resolution]] 2.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4ekv]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EKV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EKV FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BTN:BIOTIN'>BTN</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1swe\|1swe]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ekv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ekv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ekv RCSB], [http://www.ebi.ac.uk/pdbsum/4ekv PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	A novel form of tetrameric streptavidin has been engineered to have reversible biotin binding capability. In wild-type streptavidin, loop(3-4) functions as a lid for the entry and exit of biotin. When biotin is bound, interactions between biotin and key residues in loop(3-4) keep this lid in the closed state. In the engineered mutein, a second biotin exit door is created by changing the amino acid sequence of loop(7-8). This door is mobile even in the presence of the bound biotin and can facilitate the release of biotin from the mutein. Since loop(7-8) is involved in subunit interactions, alteration of this loop in the engineered mutein results in an 11 degrees rotation between the two dimers in reference to wild-type streptavidin. The tetrameric state of the engineered mutein is stabilized by a H127C mutation, which leads to the formation of inter-subunit disulfide bonds. The biotin binding kinetic parameters (k(off) of 4.28x10(-4) s(-1) and K(d) of 1.9x10(-8) M) make this engineered mutein a superb affinity agent for the purification of biotinylated biomolecules. Affinity matrices can be regenerated using gentle procedures, and regenerated matrices can be reused at least ten times without any observable reduction in binding capacity. With the combination of both the engineered mutein and wild-type streptavidin, biotinylated biomolecules can easily be affinity purified to high purity and immobilized to desirable platforms without any leakage concerns. Other potential biotechnological applications, such as development of an automated high-throughput protein purification system, are feasible.

-	<!--	+	Development of a tetrameric streptavidin mutein with reversible biotin binding capability: engineering a mobile loop as an exit door for biotin.,O'Sullivan VJ, Barrette-Ng I, Hommema E, Hermanson GT, Schofield M, Wu SC, Honetschlaeger C, Ng KK, Wong SL PLoS One. 2012;7(4):e35203. Epub 2012 Apr 19. PMID:22536357<ref>PMID:22536357</ref>
-	~~The line below this paragraph~~, ~~containing "STRUCTURE_4ekv"~~, ~~creates the "Structure Box" on the page~~.	+
-	~~You may change the PDB parameter~~ (~~which sets the PDB file loaded into the applet~~)	+
-	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~	+
-	~~or leave the SCENE parameter empty for the default display~~.	+
-	-->	+
-	~~{{STRUCTURE_4ekv\| PDB=4ekv \| SCENE= }}~~	+

-	~~===Streptavidin 8-aa-loop H127C mutein with reversible biotin binding===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

-		+	==See Also==
-	~~<!--~~	+	*[[Avidin\|Avidin]]
-	~~The line below this paragraph, {{ABSTRACT_PUBMED_22536357}}, adds the Publication Abstract to the page~~	+	== References ==
-	~~(as it appears on PubMed at http://www.pubmed.gov), where 22536357 is the PubMed ID number.~~	+	<references/>
-	~~-->~~	+	__TOC__
-	~~{{ABSTRACT_PUBMED_22536357}}~~	+	</StructureSection>
-		+
-	==~~About this Structure~~==	+
-	[[~~4ekv~~]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EKV OCA].	+
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:022536357<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Streptomyces avidinii]]		[[Category: Streptomyces avidinii]]
	[[Category: Barrette-Ng, I H.]]		[[Category: Barrette-Ng, I H.]]

OCA at 06:11, 13 June 2012

OCA — Wed, 13 Jun 2012 06:11:58 GMT

←Older revision		Revision as of 06:11, 13 June 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:4ekv.jpg\|left\|200px]]

-	The ~~entry 4ekv~~ is ~~ON HOLD~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_4ekv", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_4ekv\| PDB=4ekv \| SCENE= }}

-	~~Authors: Barrette~~-~~Ng, I.H., Honetschlaeger, C., Wong, S.L., Ng, K.K.S.~~	+	===Streptavidin 8-aa-loop H127C mutein with reversible biotin binding===

-	~~Description~~: ~~Streptavidin 8~~-aa-~~loop H127C mutein~~ with ~~reversible biotin~~ binding	+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_22536357}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 22536357 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_22536357}}
		+
		+	==About this Structure==
		+	[[4ekv]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EKV OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:022536357</ref><references group="xtra"/>
		+	[[Category: Streptomyces avidinii]]
		+	[[Category: Barrette-Ng, I H.]]
		+	[[Category: Honetschlaeger, C.]]
		+	[[Category: Ng, K K.S.]]
		+	[[Category: Wong, S L.]]
		+	[[Category: Beta-barrel]]
		+	[[Category: Binding protein]]
		+	[[Category: Biotin-binding]]
		+	[[Category: Biotin-binding protein]]

OCA: Protected "4ekv" [edit=sysop:move=sysop]

OCA — Wed, 18 Apr 2012 07:34:05 GMT

Protected "4ekv" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:34, 18 April 2012

OCA: New page: '''Unreleased structure''' The entry 4ekv is ON HOLD Authors: Barrette-Ng, I.H., Honetschlaeger, C., Wong, S.L., Ng, K.K.S. Description: Streptavidin 8-aa-loop H127C mutein with revers...

OCA — Wed, 18 Apr 2012 07:34:04 GMT

New page: '''Unreleased structure''' The entry 4ekv is ON HOLD Authors: Barrette-Ng, I.H., Honetschlaeger, C., Wong, S.L., Ng, K.K.S. Description: Streptavidin 8-aa-loop H127C mutein with revers...

New page

'''Unreleased structure'''

The entry 4ekv is ON HOLD

Authors: Barrette-Ng, I.H., Honetschlaeger, C., Wong, S.L., Ng, K.K.S.

Description: Streptavidin 8-aa-loop H127C mutein with reversible biotin binding