4elw - Revision history

OCA at 08:50, 20 March 2024

OCA — Wed, 20 Mar 2024 08:50:10 GMT

←Older revision		Revision as of 08:50, 20 March 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4elw]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ELW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ELW FirstGlance]. <br>		<table><tr><td colspan='2'>[[4elw]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ELW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ELW FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene>, <scene name='pdbligand=SIN:SUCCINIC+ACID'>SIN</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.551Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene>, <scene name='pdbligand=SIN:SUCCINIC+ACID'>SIN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4elw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4elw OCA], [https://pdbe.org/4elw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4elw RCSB], [https://www.ebi.ac.uk/pdbsum/4elw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4elw ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4elw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4elw OCA], [https://pdbe.org/4elw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4elw RCSB], [https://www.ebi.ac.uk/pdbsum/4elw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4elw ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/MENB_ECOLI MENB_ECOLI]] Converts o-succinylbenzoyl-CoA (OSB-CoA) to 1,4-dihydroxy-2-naphthoyl-CoA (DHNA-CoA).<ref>PMID:1091286</ref> <ref>PMID:20643650</ref>	+	[https://www.uniprot.org/uniprot/MENB_ECOLI MENB_ECOLI] Converts o-succinylbenzoyl-CoA (OSB-CoA) to 1,4-dihydroxy-2-naphthoyl-CoA (DHNA-CoA).<ref>PMID:1091286</ref> <ref>PMID:20643650</ref>
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	1,4-Dihydroxy-2-naphthoyl coenzyme A (DHNA-CoA) synthase, or MenB, catalyzes a carbon-carbon bond formation reaction in the biosynthesis of both vitamin K1 and K2. Bicarbonate is crucial to the activity of a large subset of its orthologues but lacks a clearly defined structural and mechanistic role. Here we determine the crystal structure of the holoenzymes from Escherichia coli at 2.30 A and Synechocystis sp. PCC6803 at 2.04 A, in which the bicarbonate cofactor is bound to the enzyme active site at a position equivalent to that of the side chain carboxylate of an aspartate residue conserved among bicarbonate-insensitive DHNA-CoA synthases. Binding of the planar anion involves both nonspecific electrostatic attraction and specific hydrogen bonding and hydrophobic interactions. In the absence of bicarbonate, the anion binding site is occupied by a chloride ion or nitrate, an inhibitor directly competing with bicarbonate. These results provide a solid structural basis for the bicarbonate dependence of the enzymatic activity of type I DHNA-CoA synthases. The unique location of the bicarbonate ion in relation to the expected position of the substrate alpha-proton in the enzyme's active site suggests a critical catalytic role for the anionic cofactor as a catalytic base in enolate formation.	+
-		+
-	Active Site Binding and Catalytic Role of Bicarbonate in 1,4-Dihydroxy-2-naphthoyl Coenzyme A Synthases from Vitamin K Biosynthetic Pathways.,Sun Y, Song H, Li J, Jiang M, Li Y, Zhou J, Guo Z Biochemistry. 2012 May 24. PMID:22606952<ref>PMID:22606952</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 4elw" style="background-color:#fffaf0;"></div~~>	+
	== References ==		== References ==
	<references/>		<references/>

OCA at 07:17, 28 September 2022

OCA — Wed, 28 Sep 2022 07:17:33 GMT

←Older revision		Revision as of 07:17, 28 September 2022
Line 1:		Line 1:

	==Structure of E. coli. 1,4-dihydroxy-2- naphthoyl coenzyme A synthases (MENB) in complex with nitrate==		==Structure of E. coli. 1,4-dihydroxy-2- naphthoyl coenzyme A synthases (MENB) in complex with nitrate==
-	<StructureSection load='4elw' size='340' side='right' caption='[[4elw]], [[Resolution\|resolution]] 2.55Å' scene=''>	+	<StructureSection load='4elw' size='340' side='right'caption='[[4elw]], [[Resolution\|resolution]] 2.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4elw]] is a 6 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Ecoli Ecoli~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ELW OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4ELW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4elw]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ELW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ELW FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene>, <scene name='pdbligand=SIN:SUCCINIC+ACID'>SIN</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene>, <scene name='pdbligand=SIN:SUCCINIC+ACID'>SIN</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4els\|4els]], [[4elx\|4elx]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4elw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4elw OCA], [https://pdbe.org/4elw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4elw RCSB], [https://www.ebi.ac.uk/pdbsum/4elw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4elw ProSAT]</span></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">menB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>~~	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/1,4-dihydroxy-2-naphthoyl-CoA_synthase 1,4-dihydroxy-2-naphthoyl-CoA synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.3.36 4.1.3.36] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4elw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4elw OCA], [~~http~~://pdbe.org/4elw PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4elw RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4elw PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4elw ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/MENB_ECOLI MENB_ECOLI]] Converts o-succinylbenzoyl-CoA (OSB-CoA) to 1,4-dihydroxy-2-naphthoyl-CoA (DHNA-CoA).<ref>PMID:1091286</ref> <ref>PMID:20643650</ref>	+	[[https://www.uniprot.org/uniprot/MENB_ECOLI MENB_ECOLI]] Converts o-succinylbenzoyl-CoA (OSB-CoA) to 1,4-dihydroxy-2-naphthoyl-CoA (DHNA-CoA).<ref>PMID:1091286</ref> <ref>PMID:20643650</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 25:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~1,4~~-~~dihydroxy-2-naphthoyl-CoA synthase~~]]	+	[[Category: Escherichia coli K-12]]
-	[[Category: ~~Ecoli~~]]	+	[[Category: Large Structures]]
-	[[Category: Guo~~, Z H~~]]	+	[[Category: Guo ZH]]
-	[[Category: Jiang, M]]	+	[[Category: Jiang M]]
-	[[Category: Li, J]]	+	[[Category: Li J]]
-	[[Category: Li, Y]]	+	[[Category: Li Y]]
-	[[Category: Song~~, H G~~]]	+	[[Category: Song HG]]
-	[[Category: Sun~~, Y R~~]]	+	[[Category: Sun YR]]
-	[[Category: Zhou~~, J H]]~~	+	[[Category: Zhou JH]]
-	~~[[Category: Dihydroxynaphthoic acid synthetase]]~~	+
-	~~[[Category: Lyase~~]]	+

OCA at 23:59, 4 August 2016

OCA — Thu, 04 Aug 2016 23:59:26 GMT

←Older revision		Revision as of 23:59, 4 August 2016
Line 1:		Line 1:
		+
	==Structure of E. coli. 1,4-dihydroxy-2- naphthoyl coenzyme A synthases (MENB) in complex with nitrate==		==Structure of E. coli. 1,4-dihydroxy-2- naphthoyl coenzyme A synthases (MENB) in complex with nitrate==
	<StructureSection load='4elw' size='340' side='right' caption='[[4elw]], [[Resolution\|resolution]] 2.55Å' scene=''>		<StructureSection load='4elw' size='340' side='right' caption='[[4elw]], [[Resolution\|resolution]] 2.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4elw]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli_k-12 Escherichia coli k-12~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ELW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ELW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4elw]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ELW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ELW FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene>, <scene name='pdbligand=SIN:SUCCINIC+ACID'>SIN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene>, <scene name='pdbligand=SIN:SUCCINIC+ACID'>SIN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4els\|4els]], [[4elx\|4elx]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4els\|4els]], [[4elx\|4elx]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">menB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ~~Escherichia coli K-12~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">menB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/1,4-dihydroxy-2-naphthoyl-CoA_synthase 1,4-dihydroxy-2-naphthoyl-CoA synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.3.36 4.1.3.36] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/1,4-dihydroxy-2-naphthoyl-CoA_synthase 1,4-dihydroxy-2-naphthoyl-CoA synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.3.36 4.1.3.36] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4elw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4elw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4elw RCSB], [http://www.ebi.ac.uk/pdbsum/4elw PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4elw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4elw OCA], [http://pdbe.org/4elw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4elw RCSB], [http://www.ebi.ac.uk/pdbsum/4elw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4elw ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 19:		Line 20:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4elw" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
Line 24:		Line 26:
	</StructureSection>		</StructureSection>
	[[Category: 1,4-dihydroxy-2-naphthoyl-CoA synthase]]		[[Category: 1,4-dihydroxy-2-naphthoyl-CoA synthase]]
-	[[Category: ~~Escherichia coli k-12~~]]	+	[[Category: Ecoli]]
	[[Category: Guo, Z H]]		[[Category: Guo, Z H]]
	[[Category: Jiang, M]]		[[Category: Jiang, M]]

OCA at 14:11, 25 December 2014

OCA — Thu, 25 Dec 2014 14:11:37 GMT

←Older revision		Revision as of 14:11, 25 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_4elw\| PDB=4elw \| SCENE= }}~~	+	==Structure of E. coli. 1,4-dihydroxy-2- naphthoyl coenzyme A synthases (MENB) in complex with nitrate==
-	===Structure of E. coli. 1,4-dihydroxy-2- naphthoyl coenzyme A synthases (MENB) in complex with nitrate===	+	<StructureSection load='4elw' size='340' side='right' caption='[[4elw]], [[Resolution\|resolution]] 2.55Å' scene=''>
-	~~{{ABSTRACT_PUBMED_22606952}}~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[4elw]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_k-12 Escherichia coli k-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ELW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ELW FirstGlance]. <br>
-	==Function==	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene>, <scene name='pdbligand=SIN:SUCCINIC+ACID'>SIN</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4els\|4els]], [[4elx\|4elx]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">menB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 Escherichia coli K-12])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/1,4-dihydroxy-2-naphthoyl-CoA_synthase 1,4-dihydroxy-2-naphthoyl-CoA synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.3.36 4.1.3.36] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4elw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4elw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4elw RCSB], [http://www.ebi.ac.uk/pdbsum/4elw PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
	[[http://www.uniprot.org/uniprot/MENB_ECOLI MENB_ECOLI]] Converts o-succinylbenzoyl-CoA (OSB-CoA) to 1,4-dihydroxy-2-naphthoyl-CoA (DHNA-CoA).<ref>PMID:1091286</ref> <ref>PMID:20643650</ref>		[[http://www.uniprot.org/uniprot/MENB_ECOLI MENB_ECOLI]] Converts o-succinylbenzoyl-CoA (OSB-CoA) to 1,4-dihydroxy-2-naphthoyl-CoA (DHNA-CoA).<ref>PMID:1091286</ref> <ref>PMID:20643650</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	1,4-Dihydroxy-2-naphthoyl coenzyme A (DHNA-CoA) synthase, or MenB, catalyzes a carbon-carbon bond formation reaction in the biosynthesis of both vitamin K1 and K2. Bicarbonate is crucial to the activity of a large subset of its orthologues but lacks a clearly defined structural and mechanistic role. Here we determine the crystal structure of the holoenzymes from Escherichia coli at 2.30 A and Synechocystis sp. PCC6803 at 2.04 A, in which the bicarbonate cofactor is bound to the enzyme active site at a position equivalent to that of the side chain carboxylate of an aspartate residue conserved among bicarbonate-insensitive DHNA-CoA synthases. Binding of the planar anion involves both nonspecific electrostatic attraction and specific hydrogen bonding and hydrophobic interactions. In the absence of bicarbonate, the anion binding site is occupied by a chloride ion or nitrate, an inhibitor directly competing with bicarbonate. These results provide a solid structural basis for the bicarbonate dependence of the enzymatic activity of type I DHNA-CoA synthases. The unique location of the bicarbonate ion in relation to the expected position of the substrate alpha-proton in the enzyme's active site suggests a critical catalytic role for the anionic cofactor as a catalytic base in enolate formation.

-	~~==About this Structure==~~	+	Active Site Binding and Catalytic Role of Bicarbonate in 1,4-Dihydroxy-2-naphthoyl Coenzyme A Synthases from Vitamin K Biosynthetic Pathways.,Sun Y, Song H, Li J, Jiang M, Li Y, Zhou J, Guo Z Biochemistry. 2012 May 24. PMID:22606952<ref>PMID:22606952</ref>
-	~~[[4elw]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_k~~-~~12 Escherichia coli k~~-~~12]. Full crystallographic information is available~~ from ~~[http://oca~~.~~weizmann~~.ac.il/~~oca-bin/ocashort?id=4ELW OCA].~~	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group="xtra">PMID:022606952</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: 1,4-dihydroxy-2-naphthoyl-CoA synthase]]		[[Category: 1,4-dihydroxy-2-naphthoyl-CoA synthase]]
	[[Category: Escherichia coli k-12]]		[[Category: Escherichia coli k-12]]
-	[[Category: Guo, Z H.]]	+	[[Category: Guo, Z H]]
-	[[Category: Jiang, M.]]	+	[[Category: Jiang, M]]
-	[[Category: Li, J.]]	+	[[Category: Li, J]]
-	[[Category: Li, Y.]]	+	[[Category: Li, Y]]
-	[[Category: Song, H G.]]	+	[[Category: Song, H G]]
-	[[Category: Sun, Y R.]]	+	[[Category: Sun, Y R]]
-	[[Category: Zhou, J H.]]	+	[[Category: Zhou, J H]]
	[[Category: Dihydroxynaphthoic acid synthetase]]		[[Category: Dihydroxynaphthoic acid synthetase]]
	[[Category: Lyase]]		[[Category: Lyase]]

OCA at 10:22, 3 July 2013

OCA — Wed, 03 Jul 2013 10:22:11 GMT

←Older revision		Revision as of 10:22, 3 July 2013
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-	[[Image:4elw.jpg\|left\|200px]]
-
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-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_4elw\| PDB=4elw \| SCENE= }}		{{STRUCTURE_4elw\| PDB=4elw \| SCENE= }}
-
	===Structure of E. coli. 1,4-dihydroxy-2- naphthoyl coenzyme A synthases (MENB) in complex with nitrate===		===Structure of E. coli. 1,4-dihydroxy-2- naphthoyl coenzyme A synthases (MENB) in complex with nitrate===
		+	{{ABSTRACT_PUBMED_22606952}}

-		+	==Function==
-	~~<!--~~	+	[[http://www.uniprot.org/uniprot/MENB_ECOLI MENB_ECOLI]] Converts o-succinylbenzoyl-CoA (OSB-CoA) to 1,4-dihydroxy-2-naphthoyl-CoA (DHNA-CoA).<ref>PMID:1091286</ref> <ref>PMID:20643650</ref>
-	~~The line below this paragraph, {{ABSTRACT_PUBMED_22606952}}, adds the Publication Abstract to the page~~	+
-	~~(as it appears on PubMed at~~ http://www.~~pubmed~~.~~gov~~), ~~where 22606952 is the PubMed ID number.~~	+
-	-->	+
-	~~{{ABSTRACT_PUBMED_22606952}}~~	+

	==About this Structure==		==About this Structure==
-	[[4elw]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli~~ Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ELW OCA].	+	[[4elw]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_k-12 Escherichia coli k-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ELW OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:022606952</ref><references group="xtra"/>	+	<ref group="xtra">PMID:022606952</ref><references group="xtra"/><references/>
-	[[Category: ~~Escherichia coli~~]]	+	[[Category: 1,4-dihydroxy-2-naphthoyl-CoA synthase]]
-	[[Category: ~~Naphthoate synthase~~]]	+	[[Category: Escherichia coli k-12]]
	[[Category: Guo, Z H.]]		[[Category: Guo, Z H.]]
	[[Category: Jiang, M.]]		[[Category: Jiang, M.]]

OCA at 07:40, 6 June 2012

OCA — Wed, 06 Jun 2012 07:40:40 GMT

←Older revision		Revision as of 07:40, 6 June 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:4elw.jpg\|left\|200px]]

-	The ~~entry 4elw~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_4elw", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_4elw\| PDB=4elw \| SCENE= }}

-	~~Authors: Sun, Y~~.R., ~~Song, H.G., Li, J., Jiang, M., Li, Y., Zhou, J.H., Guo, Z.H.~~	+	===Structure of E. coli. 1,4-dihydroxy-2- naphthoyl coenzyme A synthases (MENB) in complex with nitrate===

-	~~Description~~: ~~Structure of E~~. ~~coli~~. 1,4-~~dihydroxy~~-~~2-naphthoyl coenzyme A synthases (MENB) in complex~~ with ~~nitrate~~	+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_22606952}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 22606952 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_22606952}}
		+
		+	==About this Structure==
		+	[[4elw]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ELW OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:022606952</ref><references group="xtra"/>
		+	[[Category: Escherichia coli]]
		+	[[Category: Naphthoate synthase]]
		+	[[Category: Guo, Z H.]]
		+	[[Category: Jiang, M.]]
		+	[[Category: Li, J.]]
		+	[[Category: Li, Y.]]
		+	[[Category: Song, H G.]]
		+	[[Category: Sun, Y R.]]
		+	[[Category: Zhou, J H.]]
		+	[[Category: Dihydroxynaphthoic acid synthetase]]
		+	[[Category: Lyase]]

OCA at 08:47, 9 May 2012

OCA — Wed, 09 May 2012 08:47:51 GMT

←Older revision		Revision as of 08:47, 9 May 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4elw is ON HOLD	+	The entry 4elw is ON HOLD until Paper Publication

-	Authors: ~~SUN~~ Y. R.	+	Authors: Sun, Y.R., Song, H.G., Li, J., Jiang, M., Li, Y., Zhou, J.H., Guo, Z.H.

-	Description: Structure of E. coli. 4-dihydroxy-2-naphthoyl ~~CoA~~ synthases (~~MenB~~) in complex with nitrate	+	Description: Structure of E. coli. 1,4-dihydroxy-2-naphthoyl coenzyme A synthases (MENB) in complex with nitrate

OCA: Protected "4elw" [edit=sysop:move=sysop]

OCA — Wed, 18 Apr 2012 07:35:06 GMT

Protected "4elw" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:35, 18 April 2012

OCA: New page: '''Unreleased structure''' The entry 4elw is ON HOLD Authors: SUN Y. R. Description: Structure of E. coli. 4-dihydroxy-2-naphthoyl CoA synthases (MenB) in complex with nitrate

OCA — Wed, 18 Apr 2012 07:35:05 GMT

New page: '''Unreleased structure''' The entry 4elw is ON HOLD Authors: SUN Y. R. Description: Structure of E. coli. 4-dihydroxy-2-naphthoyl CoA synthases (MenB) in complex with nitrate

New page

'''Unreleased structure'''

The entry 4elw is ON HOLD

Authors: SUN Y. R.

Description: Structure of E. coli. 4-dihydroxy-2-naphthoyl CoA synthases (MenB) in complex with nitrate