4epa - Revision history

OCA at 07:21, 28 September 2022

OCA — Wed, 28 Sep 2022 07:21:37 GMT

←Older revision		Revision as of 07:21, 28 September 2022
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	==The crystal structure of the ferric yersiniabactin uptake receptor FyuA from Yersinia pestis==		==The crystal structure of the ferric yersiniabactin uptake receptor FyuA from Yersinia pestis==
-	<StructureSection load='4epa' size='340' side='right' caption='[[4epa]], [[Resolution\|resolution]] 3.20Å' scene=''>	+	<StructureSection load='4epa' size='340' side='right'caption='[[4epa]], [[Resolution\|resolution]] 3.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4epa]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"bacillus_pestis"_(lehmann_and_neumann_1896)_migula_1900 "bacillus~~ pestis~~" (lehmann and neumann 1896) migula 1900~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EPA OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4EPA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4epa]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Yersinia_pestis Yersinia pestis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EPA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EPA FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LDA:LAURYL+DIMETHYLAMINE-N-OXIDE'>LDA</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LDA:LAURYL+DIMETHYLAMINE-N-OXIDE'>LDA</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	~~<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat">~~<scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>~~</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4epa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4epa OCA], [https://pdbe.org/4epa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4epa RCSB], [https://www.ebi.ac.uk/pdbsum/4epa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4epa ProSAT]</span></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4epf\|4epf]], [[4epi\|4epi]]</td></tr>~~	+
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">fyuA, psn, YPO1906, y2404, YP_1649 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=632 "Bacillus pestis" (Lehmann and Neumann 1896) Migula 1900])</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4epa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4epa OCA], [~~http~~://pdbe.org/4epa PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4epa RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4epa PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4epa ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/FYUA_YERPE FYUA_YERPE]] Receptor for the bacteriocin pesticin and for the siderophore yersiniabactin.	+	[[https://www.uniprot.org/uniprot/FYUA_YERPE FYUA_YERPE]] Receptor for the bacteriocin pesticin and for the siderophore yersiniabactin.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 25:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Barnard, T J~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Buchanan, S K~~]]	+	[[Category: Yersinia pestis]]
-	[[Category: ~~Lukacik, P~~]]	+	[[Category: Barnard TJ]]
-	[[Category: ~~Iron import~~]]	+	[[Category: Buchanan SK]]
-	[[Category: ~~Metal transport]]~~	+	[[Category: Lukacik P]]
-	~~[[Category: Tonb dependent transporter~~]]	+

OCA at 11:52, 11 August 2016

OCA — Thu, 11 Aug 2016 11:52:56 GMT

←Older revision		Revision as of 11:52, 11 August 2016
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		+
	==The crystal structure of the ferric yersiniabactin uptake receptor FyuA from Yersinia pestis==		==The crystal structure of the ferric yersiniabactin uptake receptor FyuA from Yersinia pestis==
	<StructureSection load='4epa' size='340' side='right' caption='[[4epa]], [[Resolution\|resolution]] 3.20Å' scene=''>		<StructureSection load='4epa' size='340' side='right' caption='[[4epa]], [[Resolution\|resolution]] 3.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4epa]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Yersinia_pestis Yersinia~~ pestis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EPA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EPA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4epa]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_pestis"_(lehmann_and_neumann_1896)_migula_1900 "bacillus pestis" (lehmann and neumann 1896) migula 1900]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EPA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EPA FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LDA:LAURYL+DIMETHYLAMINE-N-OXIDE'>LDA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LDA:LAURYL+DIMETHYLAMINE-N-OXIDE'>LDA</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4epf\|4epf]], [[4epi\|4epi]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4epf\|4epf]], [[4epi\|4epi]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">fyuA, psn, YPO1906, y2404, YP_1649 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=632 ~~Yersinia~~ pestis])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">fyuA, psn, YPO1906, y2404, YP_1649 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=632 "Bacillus pestis" (Lehmann and Neumann 1896) Migula 1900])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4epa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4epa OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4epa RCSB], [http://www.ebi.ac.uk/pdbsum/4epa PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4epa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4epa OCA], [http://pdbe.org/4epa PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4epa RCSB], [http://www.ebi.ac.uk/pdbsum/4epa PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4epa ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/FYUA_YERPE FYUA_YERPE]] Receptor for the bacteriocin pesticin and for the siderophore yersiniabactin.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 17:		Line 20:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4epa" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Yersinia pestis]]
	[[Category: Barnard, T J]]		[[Category: Barnard, T J]]
	[[Category: Buchanan, S K]]		[[Category: Buchanan, S K]]

OCA at 14:02, 4 January 2015

OCA — Sun, 04 Jan 2015 14:02:27 GMT

←Older revision		Revision as of 14:02, 4 January 2015
Line 1:		Line 1:
-	[[~~Image:~~4epa.~~png~~\|~~left~~\|~~200px~~]]	+	==The crystal structure of the ferric yersiniabactin uptake receptor FyuA from Yersinia pestis==
		+	<StructureSection load='4epa' size='340' side='right' caption='[[4epa]], [[Resolution\|resolution]] 3.20Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4epa]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Yersinia_pestis Yersinia pestis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EPA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EPA FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LDA:LAURYL+DIMETHYLAMINE-N-OXIDE'>LDA</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4epf\|4epf]], [[4epi\|4epi]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">fyuA, psn, YPO1906, y2404, YP_1649 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=632 Yersinia pestis])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4epa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4epa OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4epa RCSB], [http://www.ebi.ac.uk/pdbsum/4epa PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Bacterial pathogens are becoming increasingly resistant to antibiotics. As an alternative therapeutic strategy, phage therapy reagents containing purified viral lysins have been developed against Gram-positive organisms but not against Gram-negative organisms due to the inability of these types of drugs to cross the bacterial outer membrane. We solved the crystal structures of a Yersinia pestis outer membrane transporter called FyuA and a bacterial toxin called pesticin that targets this transporter. FyuA is a beta-barrel membrane protein belonging to the family of TonB dependent transporters, whereas pesticin is a soluble protein with two domains, one that binds to FyuA and another that is structurally similar to phage T4 lysozyme. The structure of pesticin allowed us to design a phage therapy reagent comprised of the FyuA binding domain of pesticin fused to the N-terminus of T4 lysozyme. This hybrid toxin kills specific Yersinia and pathogenic E. coli strains and, importantly, can evade the pesticin immunity protein (Pim) giving it a distinct advantage over pesticin. Furthermore, because FyuA is required for virulence and is more common in pathogenic bacteria, the hybrid toxin also has the advantage of targeting primarily disease-causing bacteria rather than indiscriminately eliminating natural gut flora.

-	~~{{STRUCTURE_4epa\| PDB=4epa \| SCENE= }}~~	+	Structural engineering of a phage lysin that targets Gram-negative pathogens.,Lukacik P, Barnard TJ, Keller PW, Chaturvedi KS, Seddiki N, Fairman JW, Noinaj N, Kirby TL, Henderson JP, Steven AC, Hinnebusch BJ, Buchanan SK Proc Natl Acad Sci U S A. 2012 Jun 7. PMID:22679291<ref>PMID:22679291</ref>

-	~~===The crystal structure~~ of the ~~ferric yersiniabactin uptake receptor FyuA from Yersinia pestis===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_22679291}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[4epa]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Yersinia_pestis Yersinia pestis]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=4EPA OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:022679291<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Yersinia pestis]]		[[Category: Yersinia pestis]]
-	[[Category: Barnard, T J.]]	+	[[Category: Barnard, T J]]
-	[[Category: Buchanan, S K.]]	+	[[Category: Buchanan, S K]]
-	[[Category: Lukacik, P.]]	+	[[Category: Lukacik, P]]
	[[Category: Iron import]]		[[Category: Iron import]]
	[[Category: Metal transport]]		[[Category: Metal transport]]
	[[Category: Tonb dependent transporter]]		[[Category: Tonb dependent transporter]]

OCA at 06:58, 4 July 2012

OCA — Wed, 04 Jul 2012 06:58:06 GMT

←Older revision		Revision as of 06:58, 4 July 2012
Line 1:		Line 1:
-	[[Image:4epa.~~jpg~~\|left\|200px]]	+	[[Image:4epa.png\|left\|200px]]

	{{STRUCTURE_4epa\| PDB=4epa \| SCENE= }}		{{STRUCTURE_4epa\| PDB=4epa \| SCENE= }}

OCA at 07:51, 20 June 2012

OCA — Wed, 20 Jun 2012 07:51:46 GMT

←Older revision		Revision as of 07:51, 20 June 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:4epa.jpg\|left\|200px]]

-	~~The entry~~ 4epa ~~is ON HOLD~~ ~~until Paper Publication~~	+	{{STRUCTURE_4epa\| PDB=4epa \| SCENE= }}

-	~~Authors: Lukacik, P., Barnard, T.J., Buchanan, S.K.~~	+	===The crystal structure of the ferric yersiniabactin uptake receptor FyuA from Yersinia pestis===

-	~~Description: The crystal~~ structure ~~of the ferric yersiniabactin uptake receptor FyuA~~ from Yersinia pestis	+	{{ABSTRACT_PUBMED_22679291}}
		+
		+	==About this Structure==
		+	[[4epa]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Yersinia_pestis Yersinia pestis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EPA OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:022679291</ref><references group="xtra"/>
		+	[[Category: Yersinia pestis]]
		+	[[Category: Barnard, T J.]]
		+	[[Category: Buchanan, S K.]]
		+	[[Category: Lukacik, P.]]
		+	[[Category: Iron import]]
		+	[[Category: Metal transport]]
		+	[[Category: Tonb dependent transporter]]

OCA at 08:48, 9 May 2012

OCA — Wed, 09 May 2012 08:48:30 GMT

←Older revision		Revision as of 08:48, 9 May 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4epa is ON HOLD	+	The entry 4epa is ON HOLD until Paper Publication

	Authors: Lukacik, P., Barnard, T.J., Buchanan, S.K.		Authors: Lukacik, P., Barnard, T.J., Buchanan, S.K.

	Description: The crystal structure of the ferric yersiniabactin uptake receptor FyuA from Yersinia pestis		Description: The crystal structure of the ferric yersiniabactin uptake receptor FyuA from Yersinia pestis

OCA: Protected "4epa" [edit=sysop:move=sysop]

OCA — Wed, 25 Apr 2012 08:56:13 GMT

Protected "4epa" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:56, 25 April 2012

OCA: New page: '''Unreleased structure''' The entry 4epa is ON HOLD Authors: Lukacik, P., Barnard, T.J., Buchanan, S.K. Description: The crystal structure of the ferric yersiniabactin uptake receptor...

OCA — Wed, 25 Apr 2012 08:56:12 GMT

New page: '''Unreleased structure''' The entry 4epa is ON HOLD Authors: Lukacik, P., Barnard, T.J., Buchanan, S.K. Description: The crystal structure of the ferric yersiniabactin uptake receptor...

New page

'''Unreleased structure'''

The entry 4epa is ON HOLD

Authors: Lukacik, P., Barnard, T.J., Buchanan, S.K.

Description: The crystal structure of the ferric yersiniabactin uptake receptor FyuA from Yersinia pestis