4epi - Revision history

OCA at 15:06, 14 March 2024

OCA — Thu, 14 Mar 2024 15:06:34 GMT

←Older revision		Revision as of 15:06, 14 March 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4epi]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_T4 Escherichia virus T4] and [https://en.wikipedia.org/wiki/Yersinia_pestis Yersinia pestis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EPI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EPI FirstGlance]. <br>		<table><tr><td colspan='2'>[[4epi]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_T4 Escherichia virus T4] and [https://en.wikipedia.org/wiki/Yersinia_pestis Yersinia pestis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EPI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EPI FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.74Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4epi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4epi OCA], [https://pdbe.org/4epi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4epi RCSB], [https://www.ebi.ac.uk/pdbsum/4epi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4epi ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4epi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4epi OCA], [https://pdbe.org/4epi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4epi RCSB], [https://www.ebi.ac.uk/pdbsum/4epi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4epi ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/ENLYS_BPT4 ENLYS_BPT4]] Endolysin with lysozyme activity that degrades host peptidoglycans and participates with the holin and spanin proteins in the sequential events which lead to the programmed host cell lysis releasing the mature viral particles. Once the holin has permeabilized the host cell membrane, the endolysin can reach the periplasm and break down the peptidoglycan layer.<ref>PMID:22389108</ref> [[https://www.uniprot.org/uniprot/Q57159_YERPE Q57159_YERPE]]	+	[https://www.uniprot.org/uniprot/ENLYS_BPT4 ENLYS_BPT4] Endolysin with lysozyme activity that degrades host peptidoglycans and participates with the holin and spanin proteins in the sequential events which lead to the programmed host cell lysis releasing the mature viral particles. Once the holin has permeabilized the host cell membrane, the endolysin can reach the periplasm and break down the peptidoglycan layer.<ref>PMID:22389108</ref> [https://www.uniprot.org/uniprot/Q57159_YERPE Q57159_YERPE]
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	Bacterial pathogens are becoming increasingly resistant to antibiotics. As an alternative therapeutic strategy, phage therapy reagents containing purified viral lysins have been developed against Gram-positive organisms but not against Gram-negative organisms due to the inability of these types of drugs to cross the bacterial outer membrane. We solved the crystal structures of a Yersinia pestis outer membrane transporter called FyuA and a bacterial toxin called pesticin that targets this transporter. FyuA is a beta-barrel membrane protein belonging to the family of TonB dependent transporters, whereas pesticin is a soluble protein with two domains, one that binds to FyuA and another that is structurally similar to phage T4 lysozyme. The structure of pesticin allowed us to design a phage therapy reagent comprised of the FyuA binding domain of pesticin fused to the N-terminus of T4 lysozyme. This hybrid toxin kills specific Yersinia and pathogenic E. coli strains and, importantly, can evade the pesticin immunity protein (Pim) giving it a distinct advantage over pesticin. Furthermore, because FyuA is required for virulence and is more common in pathogenic bacteria, the hybrid toxin also has the advantage of targeting primarily disease-causing bacteria rather than indiscriminately eliminating natural gut flora.	+
-		+
-	Structural engineering of a phage lysin that targets Gram-negative pathogens.,Lukacik P, Barnard TJ, Keller PW, Chaturvedi KS, Seddiki N, Fairman JW, Noinaj N, Kirby TL, Henderson JP, Steven AC, Hinnebusch BJ, Buchanan SK Proc Natl Acad Sci U S A. 2012 Jun 7. PMID:22679291<ref>PMID:22679291</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 4epi" style="background-color:#fffaf0;"></div>~~	+

	==See Also==		==See Also==

OCA at 07:22, 28 September 2022

OCA — Wed, 28 Sep 2022 07:22:00 GMT

←Older revision		Revision as of 07:22, 28 September 2022
Line 1:		Line 1:

	==The crystal structure of pesticin-T4 lysozyme hybrid stabilized by engineered disulfide bonds==		==The crystal structure of pesticin-T4 lysozyme hybrid stabilized by engineered disulfide bonds==
-	<StructureSection load='4epi' size='340' side='right' caption='[[4epi]], [[Resolution\|resolution]] 1.74Å' scene=''>	+	<StructureSection load='4epi' size='340' side='right'caption='[[4epi]], [[Resolution\|resolution]] 1.74Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4epi]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"bacillus_pestis"_(lehmann_and_neumann_1896)_migula_1900 "bacillus pestis" (lehmann~~ and ~~neumann 1896) migula 1900~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EPI OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4EPI FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4epi]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_T4 Escherichia virus T4] and [https://en.wikipedia.org/wiki/Yersinia_pestis Yersinia pestis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EPI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EPI FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4epa\|4epa]], [[4epf\|4epf]], [[4exm\|4exm]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4epi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4epi OCA], [https://pdbe.org/4epi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4epi RCSB], [https://www.ebi.ac.uk/pdbsum/4epi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4epi ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pst, YP_pPCP06, YPPCP1.05c, E ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=632 "Bacillus pestis" (Lehmann and Neumann 1896) Migula 1900])</td></tr>	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4epi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4epi OCA], [~~http~~://pdbe.org/4epi PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4epi RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4epi PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4epi ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/ENLYS_BPT4 ENLYS_BPT4]] Endolysin with lysozyme activity that degrades host peptidoglycans and participates with the holin and spanin proteins in the sequential events which lead to the programmed host cell lysis releasing the mature viral particles. Once the holin has permeabilized the host cell membrane, the endolysin can reach the periplasm and break down the peptidoglycan layer.<ref>PMID:22389108</ref> [[https://www.uniprot.org/uniprot/Q57159_YERPE Q57159_YERPE]]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 26:		Line 25:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Lysozyme~~]]	+	[[Category: Escherichia virus T4]]
-	[[Category: ~~Barnard, T~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Buchanan, S K~~]]	+	[[Category: Yersinia pestis]]
-	[[Category: ~~Fairman, J W~~]]	+	[[Category: Barnard T]]
-	[[Category: ~~Lukacik, P~~]]	+	[[Category: Buchanan SK]]
-	[[Category: ~~Noinaj, N~~]]	+	[[Category: Fairman JW]]
-	[[Category: ~~Seddiki, N~~]]	+	[[Category: Lukacik P]]
-	[[Category: ~~Bacterial toxin~~]]	+	[[Category: Noinaj N]]
-	[[Category: ~~Hydrolase]]~~	+	[[Category: Seddiki N]]
-	~~[[Category: Toxin~~]]	+

OCA at 19:55, 11 August 2016

OCA — Thu, 11 Aug 2016 19:55:48 GMT

←Older revision		Revision as of 19:55, 11 August 2016
Line 1:		Line 1:
		+
	==The crystal structure of pesticin-T4 lysozyme hybrid stabilized by engineered disulfide bonds==		==The crystal structure of pesticin-T4 lysozyme hybrid stabilized by engineered disulfide bonds==
	<StructureSection load='4epi' size='340' side='right' caption='[[4epi]], [[Resolution\|resolution]] 1.74Å' scene=''>		<StructureSection load='4epi' size='340' side='right' caption='[[4epi]], [[Resolution\|resolution]] 1.74Å' scene=''>
Line 7:		Line 8:
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pst, YP_pPCP06, YPPCP1.05c, E ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=632 "Bacillus pestis" (Lehmann and Neumann 1896) Migula 1900])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pst, YP_pPCP06, YPPCP1.05c, E ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=632 "Bacillus pestis" (Lehmann and Neumann 1896) Migula 1900])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4epi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4epi OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4epi RCSB], [http://www.ebi.ac.uk/pdbsum/4epi PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4epi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4epi OCA], [http://pdbe.org/4epi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4epi RCSB], [http://www.ebi.ac.uk/pdbsum/4epi PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4epi ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4epi" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 12:48, 4 January 2015

OCA — Sun, 04 Jan 2015 12:48:51 GMT

←Older revision		Revision as of 12:48, 4 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4epi]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_pestis"_(lehmann_and_neumann_1896)_migula_1900 "bacillus pestis" (lehmann and neumann 1896) migula 1900]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EPI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EPI FirstGlance]. <br>		<table><tr><td colspan='2'>[[4epi]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_pestis"_(lehmann_and_neumann_1896)_migula_1900 "bacillus pestis" (lehmann and neumann 1896) migula 1900]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EPI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EPI FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4epa\|4epa]], [[4epf\|4epf]], [[4exm\|4exm]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4epa\|4epa]], [[4epf\|4epf]], [[4exm\|4exm]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pst, YP_pPCP06, YPPCP1.05c, E ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=632 "Bacillus pestis" (Lehmann and Neumann 1896) Migula 1900])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pst, YP_pPCP06, YPPCP1.05c, E ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=632 "Bacillus pestis" (Lehmann and Neumann 1896) Migula 1900])</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4epi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4epi OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4epi RCSB], [http://www.ebi.ac.uk/pdbsum/4epi PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4epi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4epi OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4epi RCSB], [http://www.ebi.ac.uk/pdbsum/4epi PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 25:		Line 25:
	</StructureSection>		</StructureSection>
	[[Category: Lysozyme]]		[[Category: Lysozyme]]
-	[[Category: Barnard, T.]]	+	[[Category: Barnard, T]]
-	[[Category: Buchanan, S K.]]	+	[[Category: Buchanan, S K]]
-	[[Category: Fairman, J W.]]	+	[[Category: Fairman, J W]]
-	[[Category: Lukacik, P.]]	+	[[Category: Lukacik, P]]
-	[[Category: Noinaj, N.]]	+	[[Category: Noinaj, N]]
-	[[Category: Seddiki, N.]]	+	[[Category: Seddiki, N]]
	[[Category: Bacterial toxin]]		[[Category: Bacterial toxin]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Toxin]]		[[Category: Toxin]]

OCA at 07:43, 5 June 2014

OCA — Thu, 05 Jun 2014 07:43:14 GMT

←Older revision		Revision as of 07:43, 5 June 2014
Line 1:		Line 1:
-	[[~~Image:~~4epi.~~png~~\|~~left~~\|~~200px~~]]	+	==The crystal structure of pesticin-T4 lysozyme hybrid stabilized by engineered disulfide bonds==
		+	<StructureSection load='4epi' size='340' side='right' caption='[[4epi]], [[Resolution\|resolution]] 1.74Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4epi]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_pestis"_(lehmann_and_neumann_1896)_migula_1900 "bacillus pestis" (lehmann and neumann 1896) migula 1900]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EPI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EPI FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4epa\|4epa]], [[4epf\|4epf]], [[4exm\|4exm]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pst, YP_pPCP06, YPPCP1.05c, E ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=632 "Bacillus pestis" (Lehmann and Neumann 1896) Migula 1900])</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4epi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4epi OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4epi RCSB], [http://www.ebi.ac.uk/pdbsum/4epi PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Bacterial pathogens are becoming increasingly resistant to antibiotics. As an alternative therapeutic strategy, phage therapy reagents containing purified viral lysins have been developed against Gram-positive organisms but not against Gram-negative organisms due to the inability of these types of drugs to cross the bacterial outer membrane. We solved the crystal structures of a Yersinia pestis outer membrane transporter called FyuA and a bacterial toxin called pesticin that targets this transporter. FyuA is a beta-barrel membrane protein belonging to the family of TonB dependent transporters, whereas pesticin is a soluble protein with two domains, one that binds to FyuA and another that is structurally similar to phage T4 lysozyme. The structure of pesticin allowed us to design a phage therapy reagent comprised of the FyuA binding domain of pesticin fused to the N-terminus of T4 lysozyme. This hybrid toxin kills specific Yersinia and pathogenic E. coli strains and, importantly, can evade the pesticin immunity protein (Pim) giving it a distinct advantage over pesticin. Furthermore, because FyuA is required for virulence and is more common in pathogenic bacteria, the hybrid toxin also has the advantage of targeting primarily disease-causing bacteria rather than indiscriminately eliminating natural gut flora.

-	~~{{STRUCTURE_4epi\| PDB=4epi \| SCENE= }}~~	+	Structural engineering of a phage lysin that targets Gram-negative pathogens.,Lukacik P, Barnard TJ, Keller PW, Chaturvedi KS, Seddiki N, Fairman JW, Noinaj N, Kirby TL, Henderson JP, Steven AC, Hinnebusch BJ, Buchanan SK Proc Natl Acad Sci U S A. 2012 Jun 7. PMID:22679291<ref>PMID:22679291</ref>

-	~~===The crystal structure~~ of ~~pesticin-T4 lysozyme hybrid stabilized by engineered disulfide bonds===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

-	~~{{ABSTRACT_PUBMED_22679291}}~~	+	==See Also==
-		+	*[[Lysozyme 3D structures\|Lysozyme 3D structures]]
-	==~~About this Structure~~==	+	== References ==
-	[[~~4epi~~]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Yersinia_pestis,_enterobacteria_phage_t4 Yersinia pestis, enterobacteria phage t4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EPI OCA].	+	<references/>
-		+	__TOC__
-	==~~Reference~~==	+	</StructureSection>
-	<~~ref group="xtra">PMID:022679291<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Lysozyme]]		[[Category: Lysozyme]]
-	[[Category: Yersinia pestis, enterobacteria phage t4]]
	[[Category: Barnard, T.]]		[[Category: Barnard, T.]]
	[[Category: Buchanan, S K.]]		[[Category: Buchanan, S K.]]

OCA at 06:59, 4 July 2012

OCA — Wed, 04 Jul 2012 06:59:00 GMT

←Older revision		Revision as of 06:59, 4 July 2012
Line 1:		Line 1:
-	[[Image:4epi.~~jpg~~\|left\|200px]]	+	[[Image:4epi.png\|left\|200px]]

	{{STRUCTURE_4epi\| PDB=4epi \| SCENE= }}		{{STRUCTURE_4epi\| PDB=4epi \| SCENE= }}

OCA at 07:49, 20 June 2012

OCA — Wed, 20 Jun 2012 07:49:19 GMT

←Older revision		Revision as of 07:49, 20 June 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:4epi.jpg\|left\|200px]]

-	~~The entry~~ 4epi ~~is ON HOLD~~ ~~until Paper Publication~~	+	{{STRUCTURE_4epi\| PDB=4epi \| SCENE= }}

-	~~Authors: Seddiki, N., Fairman, J.W., Noinaj, N., Lukacik, P., Barnard, T., Buchanan, S.K.~~	+	===The crystal structure of pesticin-T4 lysozyme hybrid stabilized by engineered disulfide bonds===

-	~~Description: The crystal~~ structure ~~of pesticin~~-~~T4 lysozyme hybrid stabilized by engineered disulfide bonds~~	+	{{ABSTRACT_PUBMED_22679291}}
		+
		+	==About this Structure==
		+	[[4epi]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Yersinia_pestis,_enterobacteria_phage_t4 Yersinia pestis, enterobacteria phage t4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EPI OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:022679291</ref><references group="xtra"/>
		+	[[Category: Lysozyme]]
		+	[[Category: Yersinia pestis, enterobacteria phage t4]]
		+	[[Category: Barnard, T.]]
		+	[[Category: Buchanan, S K.]]
		+	[[Category: Fairman, J W.]]
		+	[[Category: Lukacik, P.]]
		+	[[Category: Noinaj, N.]]
		+	[[Category: Seddiki, N.]]
		+	[[Category: Bacterial toxin]]
		+	[[Category: Hydrolase]]
		+	[[Category: Toxin]]

OCA at 08:48, 9 May 2012

OCA — Wed, 09 May 2012 08:48:34 GMT

←Older revision		Revision as of 08:48, 9 May 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4epi is ON HOLD	+	The entry 4epi is ON HOLD until Paper Publication

	Authors: Seddiki, N., Fairman, J.W., Noinaj, N., Lukacik, P., Barnard, T., Buchanan, S.K.		Authors: Seddiki, N., Fairman, J.W., Noinaj, N., Lukacik, P., Barnard, T., Buchanan, S.K.

	Description: The crystal structure of pesticin-T4 lysozyme hybrid stabilized by engineered disulfide bonds		Description: The crystal structure of pesticin-T4 lysozyme hybrid stabilized by engineered disulfide bonds

OCA: Protected "4epi" [edit=sysop:move=sysop]

OCA — Wed, 25 Apr 2012 08:56:26 GMT

Protected "4epi" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:56, 25 April 2012

OCA: New page: '''Unreleased structure''' The entry 4epi is ON HOLD Authors: Seddiki, N., Fairman, J.W., Noinaj, N., Lukacik, P., Barnard, T., Buchanan, S.K. Description: The crystal structure of pes...

OCA — Wed, 25 Apr 2012 08:56:25 GMT

New page: '''Unreleased structure''' The entry 4epi is ON HOLD Authors: Seddiki, N., Fairman, J.W., Noinaj, N., Lukacik, P., Barnard, T., Buchanan, S.K. Description: The crystal structure of pes...

New page

'''Unreleased structure'''

The entry 4epi is ON HOLD

Authors: Seddiki, N., Fairman, J.W., Noinaj, N., Lukacik, P., Barnard, T., Buchanan, S.K.

Description: The crystal structure of pesticin-T4 lysozyme hybrid stabilized by engineered disulfide bonds