4evz - Revision history

OCA at 15:10, 14 March 2024

OCA — Thu, 14 Mar 2024 15:10:23 GMT

←Older revision		Revision as of 15:10, 14 March 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4evz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EVZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EVZ FirstGlance]. <br>		<table><tr><td colspan='2'>[[4evz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EVZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EVZ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PI:HYDROGENPHOSPHATE+ION'>PI</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.462Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PI:HYDROGENPHOSPHATE+ION'>PI</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4evz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4evz OCA], [https://pdbe.org/4evz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4evz RCSB], [https://www.ebi.ac.uk/pdbsum/4evz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4evz ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4evz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4evz OCA], [https://pdbe.org/4evz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4evz RCSB], [https://www.ebi.ac.uk/pdbsum/4evz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4evz ProSAT]</span></td></tr>
	</table>		</table>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Due to the lack of macromolecular fossils, the enzymatic repertoire of extinct species has remained largely unknown to date. In an attempt to solve this problem, we have characterized a cyclase subunit (HisF) of the imidazole glycerol phosphate synthase (ImGP-S), which was reconstructed from the era of the last universal common ancestor of cellular organisms (LUCA). As observed for contemporary HisF proteins, the crystal structure of LUCA-HisF adopts the (betaalpha)8-barrel architecture, one of the most ancient folds. Moreover, LUCA-HisF (i) resembles extant HisF proteins with regard to internal 2-fold symmetry, active site residues, and a stabilizing salt bridge cluster, (ii) is thermostable and shows a folding mechanism similar to that of contemporary (betaalpha)8-barrel enzymes, (iii) displays high catalytic activity, and (iv) forms a stable and functional complex with the glutaminase subunit (HisH) of an extant ImGP-S. Furthermore, we show that LUCA-HisF binds to a reconstructed LUCA-HisH protein with high affinity. Our findings suggest that the evolution of highly efficient enzymes and enzyme complexes has already been completed in the LUCA era, which means that sophisticated catalytic concepts such as substrate channeling and allosteric communication existed already 3.5 billion years ago.
-
-	Evidence for the existence of elaborate enzyme complexes in the Paleoarchean era.,Reisinger B, Sperl J, Holinski A, Schmid V, Rajendran C, Carstensen L, Schlee S, Blanquart S, Merkl R, Sterner R J Am Chem Soc. 2014 Jan 8;136(1):122-9. doi: 10.1021/ja4115677. Epub 2013 Dec 23. PMID:24364418<ref>PMID:24364418</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 4evz" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 04:19, 7 October 2022

OCA — Fri, 07 Oct 2022 04:19:23 GMT

←Older revision		Revision as of 04:19, 7 October 2022
Line 3:		Line 3:
	<StructureSection load='4evz' size='340' side='right'caption='[[4evz]], [[Resolution\|resolution]] 1.46Å' scene=''>		<StructureSection load='4evz' size='340' side='right'caption='[[4evz]], [[Resolution\|resolution]] 1.46Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4evz]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EVZ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4EVZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4evz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EVZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EVZ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PI:HYDROGENPHOSPHATE+ION'>PI</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PI:HYDROGENPHOSPHATE+ION'>PI</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4evz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4evz OCA], [~~http~~://pdbe.org/4evz PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4evz RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4evz PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4evz ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4evz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4evz OCA], [https://pdbe.org/4evz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4evz RCSB], [https://www.ebi.ac.uk/pdbsum/4evz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4evz ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 21:		Line 21:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Merkl, R]]	+	[[Category: Synthetic construct]]
-	[[Category: Rajendran, C]]	+	[[Category: Merkl R]]
-	[[Category: Reisinger, B]]	+	[[Category: Rajendran C]]
-	[[Category: Sperl, J]]	+	[[Category: Reisinger B]]
-	[[Category: Sterner, R]]	+	[[Category: Sperl J]]
-	~~[[Category: Imidazole glycerol phosphate synthase-luca]]~~	+	[[Category: Sterner R]]
-	~~[[Category: Lyase~~]]	+

OCA at 07:56, 27 March 2019

OCA — Wed, 27 Mar 2019 07:56:35 GMT

←Older revision		Revision as of 07:56, 27 March 2019
Line 1:		Line 1:

	==Structure of HisF-LUCA==		==Structure of HisF-LUCA==
-	<StructureSection load='4evz' size='340' side='right' caption='[[4evz]], [[Resolution\|resolution]] 1.46Å' scene=''>	+	<StructureSection load='4evz' size='340' side='right'caption='[[4evz]], [[Resolution\|resolution]] 1.46Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4evz]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EVZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EVZ FirstGlance]. <br>		<table><tr><td colspan='2'>[[4evz]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EVZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EVZ FirstGlance]. <br>
Line 20:		Line 20:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Merkl, R]]		[[Category: Merkl, R]]
	[[Category: Rajendran, C]]		[[Category: Rajendran, C]]

OCA at 07:15, 23 December 2016

OCA — Fri, 23 Dec 2016 07:15:40 GMT

←Older revision		Revision as of 07:15, 23 December 2016
Line 1:		Line 1:
		+
	==Structure of HisF-LUCA==		==Structure of HisF-LUCA==
	<StructureSection load='4evz' size='340' side='right' caption='[[4evz]], [[Resolution\|resolution]] 1.46Å' scene=''>		<StructureSection load='4evz' size='340' side='right' caption='[[4evz]], [[Resolution\|resolution]] 1.46Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[4evz]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EVZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EVZ FirstGlance]. <br>		<table><tr><td colspan='2'>[[4evz]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EVZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EVZ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PI:HYDROGENPHOSPHATE+ION'>PI</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PI:HYDROGENPHOSPHATE+ION'>PI</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4evz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4evz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4evz RCSB], [http://www.ebi.ac.uk/pdbsum/4evz PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4evz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4evz OCA], [http://pdbe.org/4evz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4evz RCSB], [http://www.ebi.ac.uk/pdbsum/4evz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4evz ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 14:		Line 15:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4evz" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 08:20, 20 January 2015

OCA — Tue, 20 Jan 2015 08:20:59 GMT

←Older revision		Revision as of 08:20, 20 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4evz]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EVZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EVZ FirstGlance]. <br>		<table><tr><td colspan='2'>[[4evz]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EVZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EVZ FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PI:HYDROGENPHOSPHATE+ION'>PI</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PI:HYDROGENPHOSPHATE+ION'>PI</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4evz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4evz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4evz RCSB], [http://www.ebi.ac.uk/pdbsum/4evz PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4evz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4evz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4evz RCSB], [http://www.ebi.ac.uk/pdbsum/4evz PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 18:		Line 18:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Merkl, R.]]	+	[[Category: Merkl, R]]
-	[[Category: Rajendran, C.]]	+	[[Category: Rajendran, C]]
-	[[Category: Reisinger, B.]]	+	[[Category: Reisinger, B]]
-	[[Category: Sperl, J.]]	+	[[Category: Sperl, J]]
-	[[Category: Sterner, R.]]	+	[[Category: Sterner, R]]
	[[Category: Imidazole glycerol phosphate synthase-luca]]		[[Category: Imidazole glycerol phosphate synthase-luca]]
	[[Category: Lyase]]		[[Category: Lyase]]

OCA at 06:35, 24 September 2014

OCA — Wed, 24 Sep 2014 06:35:51 GMT

←Older revision		Revision as of 06:35, 24 September 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_4evz~~\| ~~PDB~~=4evz \| ~~SCENE~~= }}	+	==Structure of HisF-LUCA==
-	===~~Structure of HisF~~-~~LUCA~~===	+	<StructureSection load='4evz' size='340' side='right' caption='[[4evz]], [[Resolution\|resolution]] 1.46Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4evz]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EVZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EVZ FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PI:HYDROGENPHOSPHATE+ION'>PI</scene><br>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4evz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4evz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4evz RCSB], [http://www.ebi.ac.uk/pdbsum/4evz PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Due to the lack of macromolecular fossils, the enzymatic repertoire of extinct species has remained largely unknown to date. In an attempt to solve this problem, we have characterized a cyclase subunit (HisF) of the imidazole glycerol phosphate synthase (ImGP-S), which was reconstructed from the era of the last universal common ancestor of cellular organisms (LUCA). As observed for contemporary HisF proteins, the crystal structure of LUCA-HisF adopts the (betaalpha)8-barrel architecture, one of the most ancient folds. Moreover, LUCA-HisF (i) resembles extant HisF proteins with regard to internal 2-fold symmetry, active site residues, and a stabilizing salt bridge cluster, (ii) is thermostable and shows a folding mechanism similar to that of contemporary (betaalpha)8-barrel enzymes, (iii) displays high catalytic activity, and (iv) forms a stable and functional complex with the glutaminase subunit (HisH) of an extant ImGP-S. Furthermore, we show that LUCA-HisF binds to a reconstructed LUCA-HisH protein with high affinity. Our findings suggest that the evolution of highly efficient enzymes and enzyme complexes has already been completed in the LUCA era, which means that sophisticated catalytic concepts such as substrate channeling and allosteric communication existed already 3.5 billion years ago.

-	~~==About this Structure==~~	+	Evidence for the existence of elaborate enzyme complexes in the Paleoarchean era.,Reisinger B, Sperl J, Holinski A, Schmid V, Rajendran C, Carstensen L, Schlee S, Blanquart S, Merkl R, Sterner R J Am Chem Soc. 2014 Jan 8;136(1):122-9. doi: 10.1021/ja4115677. Epub 2013 Dec 23. PMID:24364418<ref>PMID:24364418</ref>
-	~~[[4evz]] is a 2 chain structure~~. ~~Full crystallographic information is available from [http~~://~~oca~~.~~weizmann~~.ac.il/~~oca-bin/ocashort?id~~=~~4EVZ OCA].~~	+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Merkl, R.]]		[[Category: Merkl, R.]]
	[[Category: Rajendran, C.]]		[[Category: Rajendran, C.]]

OCA at 07:39, 6 November 2013

OCA — Wed, 06 Nov 2013 07:39:27 GMT

←Older revision		Revision as of 07:39, 6 November 2013
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{STRUCTURE_4evz\| PDB=4evz \| SCENE= }}
		+	===Structure of HisF-LUCA===

-	~~The entry~~ 4evz is ~~ON HOLD until Nov 06 2014~~	+	==About this Structure==
-		+	[[4evz]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EVZ OCA].
-	~~Authors~~: ~~Reisinger, B~~.~~, Sperl, J~~., Rajendran, C., ~~Merkl, R~~., Sterner, R.	+	[[Category: Merkl, R.]]
-		+	[[Category: Rajendran, C.]]
-	~~Description~~: ~~Structure of HisF~~-~~LUCA~~	+	[[Category: Reisinger, B.]]
		+	[[Category: Sperl, J.]]
		+	[[Category: Sterner, R.]]
		+	[[Category: Imidazole glycerol phosphate synthase-luca]]
		+	[[Category: Lyase]]

OCA at 19:45, 7 August 2013

OCA — Wed, 07 Aug 2013 19:45:15 GMT

←Older revision		Revision as of 19:45, 7 August 2013
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4evz is ON HOLD until ~~Aug 07~~ 2014	+	The entry 4evz is ON HOLD until Nov 06 2014

	Authors: Reisinger, B., Sperl, J., Rajendran, C., Merkl, R., Sterner, R.		Authors: Reisinger, B., Sperl, J., Rajendran, C., Merkl, R., Sterner, R.

	Description: Structure of HisF-LUCA		Description: Structure of HisF-LUCA

OCA at 16:07, 20 March 2013

OCA — Wed, 20 Mar 2013 16:07:28 GMT

←Older revision		Revision as of 16:07, 20 March 2013
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4evz is ON HOLD until ~~Paper Publication~~	+	The entry 4evz is ON HOLD until Aug 07 2014

	Authors: Reisinger, B., Sperl, J., Rajendran, C., Merkl, R., Sterner, R.		Authors: Reisinger, B., Sperl, J., Rajendran, C., Merkl, R., Sterner, R.

	Description: Structure of HisF-LUCA		Description: Structure of HisF-LUCA

OCA at 05:29, 30 May 2012

OCA — Wed, 30 May 2012 05:29:24 GMT

←Older revision		Revision as of 05:29, 30 May 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4evz is ON HOLD	+	The entry 4evz is ON HOLD until Paper Publication

	Authors: Reisinger, B., Sperl, J., Rajendran, C., Merkl, R., Sterner, R.		Authors: Reisinger, B., Sperl, J., Rajendran, C., Merkl, R., Sterner, R.

	Description: Structure of HisF-LUCA		Description: Structure of HisF-LUCA