4f2u - Revision history

OCA at 15:14, 14 March 2024

OCA — Thu, 14 Mar 2024 15:14:48 GMT

←Older revision		Revision as of 15:14, 14 March 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4f2u]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_str._Newman Staphylococcus aureus subsp. aureus str. Newman]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F2U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4F2U FirstGlance]. <br>		<table><tr><td colspan='2'>[[4f2u]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_str._Newman Staphylococcus aureus subsp. aureus str. Newman]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F2U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4F2U FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.19Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4f2u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f2u OCA], [https://pdbe.org/4f2u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4f2u RCSB], [https://www.ebi.ac.uk/pdbsum/4f2u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4f2u ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4f2u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f2u OCA], [https://pdbe.org/4f2u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4f2u RCSB], [https://www.ebi.ac.uk/pdbsum/4f2u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4f2u ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/PLC_STAAE PLC_STAAE] Cleaves glycosylphosphatidylinositol (GPI) and phosphatidylinositol (PI) anchors but not PI phosphates. Potential virulence factor.		[https://www.uniprot.org/uniprot/PLC_STAAE PLC_STAAE] Cleaves glycosylphosphatidylinositol (GPI) and phosphatidylinositol (PI) anchors but not PI phosphates. Potential virulence factor.
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Staphylococcus aureus phosphatidylinositol-specific phospholipase C (PI-PLC) is a secreted virulence factor for this pathogenic bacterium. A novel crystal structure shows that this PI-PLC can form a dimer via helix B, a structural feature present in all secreted, bacterial PI-PLCs that is important for membrane binding. Despite the small size of this interface, it is critical for optimal enzyme activity. Kinetic evidence, increased enzyme specific activity with increasing enzyme concentration, supports a mechanism where the PI-PLC dimerization is enhanced in membranes containing phosphatidylcholine (PC). Mutagenesis of key residues confirm that the zwitterionic phospholipid acts not by specific binding to the protein, but rather by reducing anionic lipid interactions with a cationic pocket on the surface of the S. aureus enzyme that stabilizes monomeric protein. Despite its structural and sequence similarity to PI-PLCs from other Gram-positive pathogenic bacteria, S. aureus PI-PLC appears to have a unique mechanism where enzyme activity is modulated by competition between binding of soluble anions or anionic lipids to the cationic sensor and transient dimerization on the membrane.
-
-	Competition between Anion Binding and Dimerization Modulates Staphylococcus aureus Phosphatidylinositol-specific Phospholipase C Enzymatic Activity.,Cheng J, Goldstein R, Stec B, Gershenson A, Roberts MF J Biol Chem. 2012 Nov 23;287(48):40317-27. doi: 10.1074/jbc.M112.395277. Epub, 2012 Oct 4. PMID:23038258<ref>PMID:23038258</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 4f2u" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Phospholipase C\|Phospholipase C]]		*[[Phospholipase C\|Phospholipase C]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 04:30, 7 October 2022

OCA — Fri, 07 Oct 2022 04:30:25 GMT

←Older revision		Revision as of 04:30, 7 October 2022
Line 1:		Line 1:

	==Structure of the N254Y/H258Y double mutant of the Phosphatidylinositol-Specific Phospholipase C from S.aureus==		==Structure of the N254Y/H258Y double mutant of the Phosphatidylinositol-Specific Phospholipase C from S.aureus==
-	<StructureSection load='4f2u' size='340' side='right' caption='[[4f2u]], [[Resolution\|resolution]] 2.19Å' scene=''>	+	<StructureSection load='4f2u' size='340' side='right'caption='[[4f2u]], [[Resolution\|resolution]] 2.19Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4f2u]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Staae Staae~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F2U OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4F2U FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4f2u]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_str._Newman Staphylococcus aureus subsp. aureus str. Newman]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F2U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4F2U FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3v16\|3v16]], [[3v18\|3v18]], [[3v1h\|3v1h]], [[4f2b\|4f2b]], [[4f2t\|4f2t]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4f2u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f2u OCA], [https://pdbe.org/4f2u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4f2u RCSB], [https://www.ebi.ac.uk/pdbsum/4f2u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4f2u ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">plc, NWMN_0041 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=426430 STAAE])</td></tr>	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphatidylinositol_diacylglycerol-lyase Phosphatidylinositol diacylglycerol-lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.6.1.13 4.6.1.13] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4f2u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f2u OCA], [~~http~~://pdbe.org/4f2u PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4f2u RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4f2u PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4f2u ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/PLC_STAAE PLC_STAAE]] Cleaves glycosylphosphatidylinositol (GPI) and phosphatidylinositol (PI) anchors but not PI phosphates. Potential virulence factor.	+	[https://www.uniprot.org/uniprot/PLC_STAAE PLC_STAAE] Cleaves glycosylphosphatidylinositol (GPI) and phosphatidylinositol (PI) anchors but not PI phosphates. Potential virulence factor.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 28:		Line 25:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Phosphatidylinositol diacylglycerol-lyase~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Staae~~]]	+	[[Category: Staphylococcus aureus subsp. aureus str. Newman]]
-	[[Category: Cheng, J]]	+	[[Category: Cheng J]]
-	[[Category: Gershenson, A]]	+	[[Category: Gershenson A]]
-	[[Category: Goldstein, R]]	+	[[Category: Goldstein R]]
-	[[Category: Roberts~~, M F~~]]	+	[[Category: Roberts MF]]
-	[[Category: Stec, B]]	+	[[Category: Stec B]]
-	~~[[Category: Alpha beta barrel]]~~	+
-	~~[[Category: Lyase]]~~	+
-	~~[[Category: Membrane interface]]~~	+
-	~~[[Category: Phosphatidylinositol-specific phospholipase c~~]]	+

OCA at 17:28, 4 August 2016

OCA — Thu, 04 Aug 2016 17:28:08 GMT

←Older revision		Revision as of 17:28, 4 August 2016
Line 1:		Line 1:
		+
	==Structure of the N254Y/H258Y double mutant of the Phosphatidylinositol-Specific Phospholipase C from S.aureus==		==Structure of the N254Y/H258Y double mutant of the Phosphatidylinositol-Specific Phospholipase C from S.aureus==
	<StructureSection load='4f2u' size='340' side='right' caption='[[4f2u]], [[Resolution\|resolution]] 2.19Å' scene=''>		<StructureSection load='4f2u' size='340' side='right' caption='[[4f2u]], [[Resolution\|resolution]] 2.19Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4f2u]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Staphylococcus_aureus_subsp._aureus_str._newman Staphylococcus aureus subsp. aureus str. newman~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F2U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4F2U FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4f2u]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Staae Staae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F2U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4F2U FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3v16\|3v16]], [[3v18\|3v18]], [[3v1h\|3v1h]], [[4f2b\|4f2b]], [[4f2t\|4f2t]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3v16\|3v16]], [[3v18\|3v18]], [[3v1h\|3v1h]], [[4f2b\|4f2b]], [[4f2t\|4f2t]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">plc, NWMN_0041 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=426430 ~~Staphylococcus aureus subsp. aureus str. Newman~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">plc, NWMN_0041 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=426430 STAAE])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphatidylinositol_diacylglycerol-lyase Phosphatidylinositol diacylglycerol-lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.6.1.13 4.6.1.13] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphatidylinositol_diacylglycerol-lyase Phosphatidylinositol diacylglycerol-lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.6.1.13 4.6.1.13] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4f2u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f2u OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4f2u RCSB], [http://www.ebi.ac.uk/pdbsum/4f2u PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4f2u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f2u OCA], [http://pdbe.org/4f2u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4f2u RCSB], [http://www.ebi.ac.uk/pdbsum/4f2u PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4f2u ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 19:		Line 20:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4f2u" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
-	*[[~~Phosphatidylinositol-specific phospholipase~~ C\|~~Phosphatidylinositol-specific phospholipase~~ C]]	+	*[[Phospholipase C\|Phospholipase C]]
	== References ==		== References ==
	<references/>		<references/>
Line 27:		Line 29:
	</StructureSection>		</StructureSection>
	[[Category: Phosphatidylinositol diacylglycerol-lyase]]		[[Category: Phosphatidylinositol diacylglycerol-lyase]]
-	[[Category: ~~Staphylococcus aureus subsp. aureus str. newman~~]]	+	[[Category: Staae]]
	[[Category: Cheng, J]]		[[Category: Cheng, J]]
	[[Category: Gershenson, A]]		[[Category: Gershenson, A]]

OCA at 11:24, 25 December 2014

OCA — Thu, 25 Dec 2014 11:24:24 GMT

←Older revision		Revision as of 11:24, 25 December 2014
Line 9:		Line 9:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4f2u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f2u OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4f2u RCSB], [http://www.ebi.ac.uk/pdbsum/4f2u PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4f2u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f2u OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4f2u RCSB], [http://www.ebi.ac.uk/pdbsum/4f2u PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/PLC_STAAE PLC_STAAE]] Cleaves glycosylphosphatidylinositol (GPI) and phosphatidylinositol (PI) anchors but not PI phosphates. Potential virulence factor.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 15:32, 9 December 2014

OCA — Tue, 09 Dec 2014 15:32:40 GMT

←Older revision		Revision as of 15:32, 9 December 2014
Line 1:		Line 1:
-	[[~~Image:~~4f2u.~~jpg~~\|~~left~~\|~~200px~~]]	+	==Structure of the N254Y/H258Y double mutant of the Phosphatidylinositol-Specific Phospholipase C from S.aureus==
		+	<StructureSection load='4f2u' size='340' side='right' caption='[[4f2u]], [[Resolution\|resolution]] 2.19Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4f2u]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_str._newman Staphylococcus aureus subsp. aureus str. newman]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F2U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4F2U FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3v16\|3v16]], [[3v18\|3v18]], [[3v1h\|3v1h]], [[4f2b\|4f2b]], [[4f2t\|4f2t]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">plc, NWMN_0041 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=426430 Staphylococcus aureus subsp. aureus str. Newman])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphatidylinositol_diacylglycerol-lyase Phosphatidylinositol diacylglycerol-lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.6.1.13 4.6.1.13] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4f2u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f2u OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4f2u RCSB], [http://www.ebi.ac.uk/pdbsum/4f2u PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Staphylococcus aureus phosphatidylinositol-specific phospholipase C (PI-PLC) is a secreted virulence factor for this pathogenic bacterium. A novel crystal structure shows that this PI-PLC can form a dimer via helix B, a structural feature present in all secreted, bacterial PI-PLCs that is important for membrane binding. Despite the small size of this interface, it is critical for optimal enzyme activity. Kinetic evidence, increased enzyme specific activity with increasing enzyme concentration, supports a mechanism where the PI-PLC dimerization is enhanced in membranes containing phosphatidylcholine (PC). Mutagenesis of key residues confirm that the zwitterionic phospholipid acts not by specific binding to the protein, but rather by reducing anionic lipid interactions with a cationic pocket on the surface of the S. aureus enzyme that stabilizes monomeric protein. Despite its structural and sequence similarity to PI-PLCs from other Gram-positive pathogenic bacteria, S. aureus PI-PLC appears to have a unique mechanism where enzyme activity is modulated by competition between binding of soluble anions or anionic lipids to the cationic sensor and transient dimerization on the membrane.

-	~~{{STRUCTURE_4f2u\| PDB=4f2u \| SCENE= }}~~	+	Competition between Anion Binding and Dimerization Modulates Staphylococcus aureus Phosphatidylinositol-specific Phospholipase C Enzymatic Activity.,Cheng J, Goldstein R, Stec B, Gershenson A, Roberts MF J Biol Chem. 2012 Nov 23;287(48):40317-27. doi: 10.1074/jbc.M112.395277. Epub, 2012 Oct 4. PMID:23038258<ref>PMID:23038258</ref>

-	~~===Structure of the N254Y~~/~~H258Y double mutant~~ of the ~~Phosphatidylinositol-Specific Phospholipase C from~~ S.~~aureus===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

-	~~{{ABSTRACT_PUBMED_23038258}}~~	+	==See Also==
-		+	*[[Phosphatidylinositol-specific phospholipase C\|Phosphatidylinositol-specific phospholipase C]]
-	==~~About this Structure~~==	+	== References ==
-	[[~~4f2u~~]] ~~is a 1 chain structure with sequence from [http:~~//en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_str._newman Staphylococcus aureus subsp. aureus str. newman]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F2U OCA].	+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Phosphatidylinositol diacylglycerol-lyase]]		[[Category: Phosphatidylinositol diacylglycerol-lyase]]
	[[Category: Staphylococcus aureus subsp. aureus str. newman]]		[[Category: Staphylococcus aureus subsp. aureus str. newman]]
-	[[Category: Cheng, J.]]	+	[[Category: Cheng, J]]
-	[[Category: Gershenson, A.]]	+	[[Category: Gershenson, A]]
-	[[Category: Goldstein, R.]]	+	[[Category: Goldstein, R]]
-	[[Category: Roberts, M F.]]	+	[[Category: Roberts, M F]]
-	[[Category: Stec, B.]]	+	[[Category: Stec, B]]
	[[Category: Alpha beta barrel]]		[[Category: Alpha beta barrel]]
	[[Category: Lyase]]		[[Category: Lyase]]
	[[Category: Membrane interface]]		[[Category: Membrane interface]]
	[[Category: Phosphatidylinositol-specific phospholipase c]]		[[Category: Phosphatidylinositol-specific phospholipase c]]

OCA at 21:09, 12 December 2012

OCA — Wed, 12 Dec 2012 21:09:13 GMT

←Older revision		Revision as of 21:09, 12 December 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:4f2u.jpg\|left\|200px]]

-	~~The entry~~ 4f2u ~~is ON HOLD~~ ~~until Paper Publication~~	+	{{STRUCTURE_4f2u\| PDB=4f2u \| SCENE= }}

-	~~Authors: Cheng, J., Goldstein, R., Stec, B., Gershenson, A., Roberts, M.F~~.	+	===Structure of the N254Y/H258Y double mutant of the Phosphatidylinositol-Specific Phospholipase C from S.aureus===

-	~~Description:~~ Structure ~~of the N254Y~~/~~H258Y double mutant of the~~ Phosphatidylinositol-~~Specific Phospholipase C from S~~.aureus	+	{{ABSTRACT_PUBMED_23038258}}
		+
		+	==About this Structure==
		+	[[4f2u]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_str._newman Staphylococcus aureus subsp. aureus str. newman]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F2U OCA].
		+	[[Category: Phosphatidylinositol diacylglycerol-lyase]]
		+	[[Category: Staphylococcus aureus subsp. aureus str. newman]]
		+	[[Category: Cheng, J.]]
		+	[[Category: Gershenson, A.]]
		+	[[Category: Goldstein, R.]]
		+	[[Category: Roberts, M F.]]
		+	[[Category: Stec, B.]]
		+	[[Category: Alpha beta barrel]]
		+	[[Category: Lyase]]
		+	[[Category: Membrane interface]]
		+	[[Category: Phosphatidylinositol-specific phospholipase c]]

OCA at 04:46, 12 September 2012

OCA — Wed, 12 Sep 2012 04:46:34 GMT

←Older revision		Revision as of 04:46, 12 September 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4f2u is ON HOLD	+	The entry 4f2u is ON HOLD until Paper Publication

	Authors: Cheng, J., Goldstein, R., Stec, B., Gershenson, A., Roberts, M.F.		Authors: Cheng, J., Goldstein, R., Stec, B., Gershenson, A., Roberts, M.F.

	Description: Structure of the N254Y/H258Y double mutant of the Phosphatidylinositol-Specific Phospholipase C from S.aureus		Description: Structure of the N254Y/H258Y double mutant of the Phosphatidylinositol-Specific Phospholipase C from S.aureus

OCA: Protected "4f2u" [edit=sysop:move=sysop]

OCA — Wed, 16 May 2012 08:54:44 GMT

Protected "4f2u" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:54, 16 May 2012

OCA: New page: '''Unreleased structure''' The entry 4f2u is ON HOLD Authors: Cheng, J., Goldstein, R., Stec, B., Gershenson, A., Roberts, M.F. Description: Structure of the N254Y/H258Y double mutant ...

OCA — Wed, 16 May 2012 08:54:43 GMT

New page: '''Unreleased structure''' The entry 4f2u is ON HOLD Authors: Cheng, J., Goldstein, R., Stec, B., Gershenson, A., Roberts, M.F. Description: Structure of the N254Y/H258Y double mutant ...

New page

'''Unreleased structure'''

The entry 4f2u is ON HOLD

Authors: Cheng, J., Goldstein, R., Stec, B., Gershenson, A., Roberts, M.F.

Description: Structure of the N254Y/H258Y double mutant of the Phosphatidylinositol-Specific Phospholipase C from S.aureus