4f4k - Revision history

OCA at 15:16, 14 March 2024

OCA — Thu, 14 Mar 2024 15:16:07 GMT

←Older revision		Revision as of 15:16, 14 March 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4f4k]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Geobacillus_kaustophilus_HTA426 Geobacillus kaustophilus HTA426] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F4K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4F4K FirstGlance]. <br>		<table><tr><td colspan='2'>[[4f4k]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Geobacillus_kaustophilus_HTA426 Geobacillus kaustophilus HTA426] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F4K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4F4K FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DDG:2,3-DIDEOXY-GUANOSINE-5-MONOPHOSPHATE'>DDG</scene>, <scene name='pdbligand=DG3:2-3-DIDEOXYGUANOSINE-5-TRIPHOSPHATE'>DG3</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.6Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DDG:2,3-DIDEOXY-GUANOSINE-5-MONOPHOSPHATE'>DDG</scene>, <scene name='pdbligand=DG3:2-3-DIDEOXYGUANOSINE-5-TRIPHOSPHATE'>DG3</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4f4k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f4k OCA], [https://pdbe.org/4f4k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4f4k RCSB], [https://www.ebi.ac.uk/pdbsum/4f4k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4f4k ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4f4k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f4k OCA], [https://pdbe.org/4f4k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4f4k RCSB], [https://www.ebi.ac.uk/pdbsum/4f4k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4f4k ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/Q5KWC1_GEOKA Q5KWC1_GEOKA]		[https://www.uniprot.org/uniprot/Q5KWC1_GEOKA Q5KWC1_GEOKA]
		+
		+	==See Also==
		+	*[[DNA polymerase 3D structures\|DNA polymerase 3D structures]]
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 04:34, 7 October 2022

OCA — Fri, 07 Oct 2022 04:34:32 GMT

←Older revision		Revision as of 04:34, 7 October 2022
Line 1:		Line 1:

	==DNA Polymerase I Large Fragment Complex 6==		==DNA Polymerase I Large Fragment Complex 6==
-	<StructureSection load='4f4k' size='340' side='right' caption='[[4f4k]], [[Resolution\|resolution]] 1.60Å' scene=''>	+	<StructureSection load='4f4k' size='340' side='right'caption='[[4f4k]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4f4k]] is a 6 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Geoka Geoka~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F4K OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4F4K FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4f4k]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Geobacillus_kaustophilus_HTA426 Geobacillus kaustophilus HTA426] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F4K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4F4K FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DG3:2-3-DIDEOXYGUANOSINE-5-TRIPHOSPHATE'>DG3</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DDG:2,3-DIDEOXY-GUANOSINE-5-MONOPHOSPHATE'>DDG</scene>, <scene name='pdbligand=DG3:2-3-DIDEOXYGUANOSINE-5-TRIPHOSPHATE'>DG3</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	~~<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=DDG:2,3-DIDEOXY-GUANOSINE-5-MONOPHOSPHATE'>DDG</scene></td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4f4k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f4k OCA], [https://pdbe.org/4f4k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4f4k RCSB], [https://www.ebi.ac.uk/pdbsum/4f4k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4f4k ProSAT]</span></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ez6\|4ez6]], [[4f2r\|4f2r]], [[4f2s\|4f2s]], [[4ez9\|4ez9]], [[4f3o\|4f3o]], [[4f8r\|4f8r]]</td></tr>	+
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">polA, GK2730 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=235909 GEOKA])</td></tr>	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4f4k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f4k OCA], [~~http~~://pdbe.org/4f4k PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4f4k RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4f4k PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4f4k ProSAT]</span></td></tr>	+
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/Q5KWC1_GEOKA Q5KWC1_GEOKA]
-	Even though high-fidelity polymerases copy DNA with remarkable accuracy, some base-pair mismatches are incorporated at low frequency, leading to spontaneous mutagenesis. Using high-resolution X-ray crystallographic analysis of a DNA polymerase that catalyzes replication in crystals, we observe that a C*A mismatch can mimic the shape of cognate base pairs at the site of incorporation. This shape mimicry enables the mismatch to evade the error detection mechanisms of the polymerase, which would normally either prevent mismatch incorporation or promote its nucleolytic excision. Movement of a single proton on one of the mismatched bases alters the hydrogen-bonding pattern such that a base pair forms with an overall shape that is virtually indistinguishable from a canonical, Watson-Crick base pair in double-stranded DNA. These observations provide structural evidence for the rare tautomer hypothesis of spontaneous mutagenesis, a long-standing concept that has been difficult to demonstrate directly.	+
-		+
-	Structural evidence for the rare tautomer hypothesis of spontaneous mutagenesis.,Wang W, Hellinga HW, Beese LS Proc Natl Acad Sci U S A. 2011 Oct 25;108(43):17644-8. Epub 2011 Oct 17. PMID:22006298<ref>PMID:~~22006298<~~/~~ref>~~	+
-		+
-	~~From MEDLINE®~~/~~PubMed®, a database of the U~~.S. ~~National Library of Medicine.<br>~~	+
-	</~~div>~~	+
-	~~<div class="pdbe-citations 4f4k" style="background-color:#fffaf0;"><~~/~~div>~~	+
-		+
-	~~==See Also==~~	+
-	*[[DNA polymerase\|DNA polymerase]]	+
-	~~== References ==~~	+
-	~~<references/>~~	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~DNA-directed DNA polymerase~~]]	+	[[Category: Geobacillus kaustophilus HTA426]]
-	[[Category: ~~Geoka~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Beese, L S~~]]	+	[[Category: Synthetic construct]]
-	[[Category: ~~Wang, W~~]]	+	[[Category: Beese LS]]
-	[[Category: ~~Closed form]]~~	+	[[Category: Wang W]]
-	~~[[Category: Dna polymerase i]]~~	+
-	~~[[Category: Protein-dna complex]]~~	+
-	~~[[Category: Transferase-dna complex~~]]	+

OCA at 20:15, 4 August 2016

OCA — Thu, 04 Aug 2016 20:15:20 GMT

←Older revision		Revision as of 20:15, 4 August 2016
Line 1:		Line 1:
		+
	==DNA Polymerase I Large Fragment Complex 6==		==DNA Polymerase I Large Fragment Complex 6==
	<StructureSection load='4f4k' size='340' side='right' caption='[[4f4k]], [[Resolution\|resolution]] 1.60Å' scene=''>		<StructureSection load='4f4k' size='340' side='right' caption='[[4f4k]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4f4k]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Geobacillus_kaustophilus_hta426 Geobacillus kaustophilus hta426~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F4K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4F4K FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4f4k]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Geoka Geoka]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F4K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4F4K FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DG3:2-3-DIDEOXYGUANOSINE-5-TRIPHOSPHATE'>DG3</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DG3:2-3-DIDEOXYGUANOSINE-5-TRIPHOSPHATE'>DG3</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=DDG:2,3-DIDEOXY-GUANOSINE-5-MONOPHOSPHATE'>DDG</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=DDG:2,3-DIDEOXY-GUANOSINE-5-MONOPHOSPHATE'>DDG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ez6\|4ez6]], [[4f2r\|4f2r]], [[4f2s\|4f2s]], [[4ez9\|4ez9]], [[4f3o\|4f3o]], [[4f8r\|4f8r]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ez6\|4ez6]], [[4f2r\|4f2r]], [[4f2s\|4f2s]], [[4ez9\|4ez9]], [[4f3o\|4f3o]], [[4f8r\|4f8r]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">polA, GK2730 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=235909 ~~Geobacillus kaustophilus HTA426~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">polA, GK2730 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=235909 GEOKA])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4f4k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f4k OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4f4k RCSB], [http://www.ebi.ac.uk/pdbsum/4f4k PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4f4k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f4k OCA], [http://pdbe.org/4f4k PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4f4k RCSB], [http://www.ebi.ac.uk/pdbsum/4f4k PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4f4k ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4f4k" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 26:		Line 28:
	</StructureSection>		</StructureSection>
	[[Category: DNA-directed DNA polymerase]]		[[Category: DNA-directed DNA polymerase]]
-	[[Category: ~~Geobacillus kaustophilus hta426~~]]	+	[[Category: Geoka]]
	[[Category: Beese, L S]]		[[Category: Beese, L S]]
	[[Category: Wang, W]]		[[Category: Wang, W]]

OCA at 10:54, 21 December 2014

OCA — Sun, 21 Dec 2014 10:54:47 GMT

←Older revision		Revision as of 10:54, 21 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_4f4k~~\| ~~PDB~~=4f4k \| ~~SCENE~~= }}	+	==DNA Polymerase I Large Fragment Complex 6==
-	===DNA ~~Polymerase I Large Fragment Complex 6~~===	+	<StructureSection load='4f4k' size='340' side='right' caption='[[4f4k]], [[Resolution\|resolution]] 1.60Å' scene=''>
-	~~{{ABSTRACT_PUBMED_22006298}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4f4k]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Geobacillus_kaustophilus_hta426 Geobacillus kaustophilus hta426]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F4K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4F4K FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DG3:2-3-DIDEOXYGUANOSINE-5-TRIPHOSPHATE'>DG3</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=DDG:2,3-DIDEOXY-GUANOSINE-5-MONOPHOSPHATE'>DDG</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ez6\|4ez6]], [[4f2r\|4f2r]], [[4f2s\|4f2s]], [[4ez9\|4ez9]], [[4f3o\|4f3o]], [[4f8r\|4f8r]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">polA, GK2730 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=235909 Geobacillus kaustophilus HTA426])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4f4k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f4k OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4f4k RCSB], [http://www.ebi.ac.uk/pdbsum/4f4k PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Even though high-fidelity polymerases copy DNA with remarkable accuracy, some base-pair mismatches are incorporated at low frequency, leading to spontaneous mutagenesis. Using high-resolution X-ray crystallographic analysis of a DNA polymerase that catalyzes replication in crystals, we observe that a C*A mismatch can mimic the shape of cognate base pairs at the site of incorporation. This shape mimicry enables the mismatch to evade the error detection mechanisms of the polymerase, which would normally either prevent mismatch incorporation or promote its nucleolytic excision. Movement of a single proton on one of the mismatched bases alters the hydrogen-bonding pattern such that a base pair forms with an overall shape that is virtually indistinguishable from a canonical, Watson-Crick base pair in double-stranded DNA. These observations provide structural evidence for the rare tautomer hypothesis of spontaneous mutagenesis, a long-standing concept that has been difficult to demonstrate directly.

-	~~==About this Structure==~~	+	Structural evidence for the rare tautomer hypothesis of spontaneous mutagenesis.,Wang W, Hellinga HW, Beese LS Proc Natl Acad Sci U S A. 2011 Oct 25;108(43):17644-8. Epub 2011 Oct 17. PMID:22006298<ref>PMID:22006298</ref>
-	~~[[4f4k]] is a 6 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Geobacillus_kaustophilus_hta426 Geobacillus kaustophilus hta426]. Full crystallographic information is available from [http~~:~~//oca~~.~~weizmann~~.~~ac.il~~/~~oca-bin/ocashort?id=4F4K OCA].~~	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group~~=~~"xtra">PMID:022006298</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
		+
		+	==See Also==
		+	*[[DNA polymerase\|DNA polymerase]]
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: DNA-directed DNA polymerase]]		[[Category: DNA-directed DNA polymerase]]
	[[Category: Geobacillus kaustophilus hta426]]		[[Category: Geobacillus kaustophilus hta426]]
-	[[Category: Beese, L S.]]	+	[[Category: Beese, L S]]
-	[[Category: Wang, W.]]	+	[[Category: Wang, W]]
	[[Category: Closed form]]		[[Category: Closed form]]
	[[Category: Dna polymerase i]]		[[Category: Dna polymerase i]]
	[[Category: Protein-dna complex]]		[[Category: Protein-dna complex]]
	[[Category: Transferase-dna complex]]		[[Category: Transferase-dna complex]]

OCA at 13:02, 24 July 2013

OCA — Wed, 24 Jul 2013 13:02:11 GMT

←Older revision		Revision as of 13:02, 24 July 2013
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{STRUCTURE_4f4k\| PDB=4f4k \| SCENE= }}
		+	===DNA Polymerase I Large Fragment Complex 6===
		+	{{ABSTRACT_PUBMED_22006298}}

-	~~The entry~~ 4f4k is ~~ON HOLD until Jul 18 2014~~	+	==About this Structure==
		+	[[4f4k]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Geobacillus_kaustophilus_hta426 Geobacillus kaustophilus hta426]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F4K OCA].

-	~~Authors~~: ~~Wang, W.,~~ Beese, L.S.	+	==Reference==
-		+	<ref group="xtra">PMID:022006298</ref><references group="xtra"/><references/>
-	~~Description~~: ~~DNA Polymerase I Large Fragment Complex 6~~	+	[[Category: DNA-directed DNA polymerase]]
		+	[[Category: Geobacillus kaustophilus hta426]]
		+	[[Category: Beese, L S.]]
		+	[[Category: Wang, W.]]
		+	[[Category: Closed form]]
		+	[[Category: Dna polymerase i]]
		+	[[Category: Protein-dna complex]]
		+	[[Category: Transferase-dna complex]]

OCA at 04:41, 18 July 2013

OCA — Thu, 18 Jul 2013 04:41:42 GMT

←Older revision		Revision as of 04:41, 18 July 2013
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4f4k is ON HOLD until Jul 15 2014	+	The entry 4f4k is ON HOLD until Jul 18 2014

	Authors: Wang, W., Beese, L.S.		Authors: Wang, W., Beese, L.S.

	Description: DNA Polymerase I Large Fragment Complex 6		Description: DNA Polymerase I Large Fragment Complex 6

OCA at 10:59, 19 June 2013

OCA — Wed, 19 Jun 2013 10:59:53 GMT

←Older revision		Revision as of 10:59, 19 June 2013
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4f4k is ON HOLD until ~~Paper Publication~~	+	The entry 4f4k is ON HOLD until Jul 15 2014

	Authors: Wang, W., Beese, L.S.		Authors: Wang, W., Beese, L.S.

	Description: DNA Polymerase I Large Fragment Complex 6		Description: DNA Polymerase I Large Fragment Complex 6

OCA at 05:46, 13 June 2012

OCA — Wed, 13 Jun 2012 05:46:38 GMT

←Older revision		Revision as of 05:46, 13 June 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4f4k is ON HOLD	+	The entry 4f4k is ON HOLD until Paper Publication

	Authors: Wang, W., Beese, L.S.		Authors: Wang, W., Beese, L.S.

	Description: DNA Polymerase I Large Fragment Complex 6		Description: DNA Polymerase I Large Fragment Complex 6

OCA: Protected "4f4k" [edit=sysop:move=sysop]

OCA — Wed, 16 May 2012 08:56:28 GMT

Protected "4f4k" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:56, 16 May 2012

OCA: New page: '''Unreleased structure''' The entry 4f4k is ON HOLD Authors: Wang, W., Beese, L.S. Description: DNA Polymerase I Large Fragment Complex 6

OCA — Wed, 16 May 2012 08:56:27 GMT

New page: '''Unreleased structure''' The entry 4f4k is ON HOLD Authors: Wang, W., Beese, L.S. Description: DNA Polymerase I Large Fragment Complex 6

New page

'''Unreleased structure'''

The entry 4f4k is ON HOLD

Authors: Wang, W., Beese, L.S.

Description: DNA Polymerase I Large Fragment Complex 6