4f61 - Revision history

OCA at 15:17, 14 March 2024

OCA — Thu, 14 Mar 2024 15:17:06 GMT

←Older revision		Revision as of 15:17, 14 March 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4f61]] is a 9 chain structure with sequence from [https://en.wikipedia.org/wiki/Ovis_aries Ovis aries] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F61 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4F61 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4f61]] is a 9 chain structure with sequence from [https://en.wikipedia.org/wiki/Ovis_aries Ovis aries] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F61 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4F61 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 4.17Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4f61 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f61 OCA], [https://pdbe.org/4f61 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4f61 RCSB], [https://www.ebi.ac.uk/pdbsum/4f61 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4f61 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4f61 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f61 OCA], [https://pdbe.org/4f61 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4f61 RCSB], [https://www.ebi.ac.uk/pdbsum/4f61 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4f61 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/D0VWZ0_SHEEP D0VWZ0_SHEEP] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain (By similarity).[RuleBase:RU003505][SAAS:SAAS023123_004_019801]		[https://www.uniprot.org/uniprot/D0VWZ0_SHEEP D0VWZ0_SHEEP] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain (By similarity).[RuleBase:RU003505][SAAS:SAAS023123_004_019801]
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	In cells, microtubule dynamics is regulated by stabilizing and destabilizing factors. Whereas proteins in both categories have been identified, their mechanism of action is rarely understood at the molecular level. This is due in part to the difficulties faced in structural approaches to obtain atomic models when tubulin is involved. Here, we design and characterize new stathmin-like domain (SLD) proteins that sequester tubulins in numbers different from two, the number of tubulins bound by stathmin or by the SLD of RB3, two stathmin family members that have been extensively studied. We established rules for the design of tight tubulin-SLD assemblies and applied them to complexes containing one to four tubulin heterodimers. Biochemical and structural experiments showed that the engineered SLDs behaved as expected. The new SLDs will be tools for structural studies of microtubule regulation. The larger complexes will be useful for cryo-electron microscopy, whereas crystallography or nuclear magnetic resonance will benefit from the 1:1 tubulin-SLD assembly. Finally, our results provide new insight into SLD function, suggesting that a major effect of these phosphorylatable proteins is the programmed release of sequestered tubulin for microtubule assembly at the specific cellular locations of members of the stathmin family.
-
-	Design and characterization of modular scaffolds for tubulin assembly.,Mignot I, Pecqueur L, Dorleans A, Karuppasamy M, Ravelli RB, Dreier B, Pluckthun A, Knossow M, Gigant B J Biol Chem. 2012 Sep 7;287(37):31085-94. Epub 2012 Jul 12. PMID:22791712<ref>PMID:22791712</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 4f61" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
		+	*[[Stathmin-4 3D structures\|Stathmin-4 3D structures]]
	*[[Tubulin 3D Structures\|Tubulin 3D Structures]]		*[[Tubulin 3D Structures\|Tubulin 3D Structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 04:37, 7 October 2022

OCA — Fri, 07 Oct 2022 04:37:10 GMT

←Older revision		Revision as of 04:37, 7 October 2022
Line 3:		Line 3:
	<StructureSection load='4f61' size='340' side='right'caption='[[4f61]], [[Resolution\|resolution]] 4.17Å' scene=''>		<StructureSection load='4f61' size='340' side='right'caption='[[4f61]], [[Resolution\|resolution]] 4.17Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4f61]] is a 9 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Ovis_aries Ovis aries] and [~~http~~://en.wikipedia.org/wiki/~~Synthetic_construct_sequences~~ Synthetic construct ~~sequences~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F61 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4F61 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4f61]] is a 9 chain structure with sequence from [https://en.wikipedia.org/wiki/Ovis_aries Ovis aries] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F61 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4F61 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4f6r\|4f6r]]~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4f61 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f61 OCA], [https://pdbe.org/4f61 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4f61 RCSB], [https://www.ebi.ac.uk/pdbsum/4f61 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4f61 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4f61 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f61 OCA], [~~http~~://pdbe.org/4f61 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4f61 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4f61 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4f61 ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/D0VWZ0_SHEEP D0VWZ0_SHEEP]] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain (By similarity).[RuleBase:RU003505][SAAS:SAAS023123_004_019801] [[http://www.uniprot.org/uniprot/D0VWY9_SHEEP D0VWY9_SHEEP]] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain (By similarity).[RuleBase:RU003505]	+	[https://www.uniprot.org/uniprot/D0VWZ0_SHEEP D0VWZ0_SHEEP] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain (By similarity).[RuleBase:RU003505][SAAS:SAAS023123_004_019801]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 20:

	==See Also==		==See Also==
-	*[[Tubulin\|Tubulin]]	+	*[[Tubulin 3D Structures\|Tubulin 3D Structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 28:		Line 27:
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Ovis aries]]		[[Category: Ovis aries]]
-	[[Category: Synthetic construct ~~sequences~~]]	+	[[Category: Synthetic construct]]
-	[[Category: Gigant, B]]	+	[[Category: Gigant B]]
-	[[Category: Knossow, M]]	+	[[Category: Knossow M]]
-	[[Category: Mignot, I]]	+	[[Category: Mignot I]]
-	~~[[Category: Alpha-tubulin]]~~	+
-	~~[[Category: Beta-tubulin]]~~	+
-	~~[[Category: Cell cycle]]~~	+
-	~~[[Category: Gtpase]]~~	+
-	~~[[Category: Microtubule]]~~	+
-	~~[[Category: Rb3]]~~	+
-	~~[[Category: Stathmin tubulin~~]]	+

OCA at 08:12, 27 March 2019

OCA — Wed, 27 Mar 2019 08:12:02 GMT

←Older revision		Revision as of 08:12, 27 March 2019
Line 1:		Line 1:

	==Tubulin:Stathmin-like domain complex==		==Tubulin:Stathmin-like domain complex==
-	<StructureSection load='4f61' size='340' side='right' caption='[[4f61]], [[Resolution\|resolution]] 4.17Å' scene=''>	+	<StructureSection load='4f61' size='340' side='right'caption='[[4f61]], [[Resolution\|resolution]] 4.17Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4f61]] is a 9 chain structure with sequence from [http://en.wikipedia.org/wiki/Ovis_aries Ovis aries] and [http://en.wikipedia.org/wiki/Synthetic_construct_sequences Synthetic construct sequences]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F61 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4F61 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4f61]] is a 9 chain structure with sequence from [http://en.wikipedia.org/wiki/Ovis_aries Ovis aries] and [http://en.wikipedia.org/wiki/Synthetic_construct_sequences Synthetic construct sequences]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F61 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4F61 FirstGlance]. <br>
Line 26:		Line 26:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Ovis aries]]		[[Category: Ovis aries]]
	[[Category: Synthetic construct sequences]]		[[Category: Synthetic construct sequences]]

OCA at 04:58, 5 August 2016

OCA — Fri, 05 Aug 2016 04:58:56 GMT

←Older revision		Revision as of 04:58, 5 August 2016
Line 1:		Line 1:
		+
	==Tubulin:Stathmin-like domain complex==		==Tubulin:Stathmin-like domain complex==
	<StructureSection load='4f61' size='340' side='right' caption='[[4f61]], [[Resolution\|resolution]] 4.17Å' scene=''>		<StructureSection load='4f61' size='340' side='right' caption='[[4f61]], [[Resolution\|resolution]] 4.17Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4f61]] is a 9 chain structure with sequence from [http://en.wikipedia.org/wiki/Ovis_aries Ovis aries] and [http://en.wikipedia.org/wiki/~~Synthetic_construct~~ Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F61 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4F61 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4f61]] is a 9 chain structure with sequence from [http://en.wikipedia.org/wiki/Ovis_aries Ovis aries] and [http://en.wikipedia.org/wiki/Synthetic_construct_sequences Synthetic construct sequences]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F61 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4F61 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4f6r\|4f6r]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4f6r\|4f6r]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4f61 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f61 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4f61 RCSB], [http://www.ebi.ac.uk/pdbsum/4f61 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4f61 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f61 OCA], [http://pdbe.org/4f61 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4f61 RCSB], [http://www.ebi.ac.uk/pdbsum/4f61 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4f61 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4f61" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 25:		Line 27:
	</StructureSection>		</StructureSection>
	[[Category: Ovis aries]]		[[Category: Ovis aries]]
-	[[Category: Synthetic construct]]	+	[[Category: Synthetic construct sequences]]
	[[Category: Gigant, B]]		[[Category: Gigant, B]]
	[[Category: Knossow, M]]		[[Category: Knossow, M]]

OCA at 15:57, 25 December 2014

OCA — Thu, 25 Dec 2014 15:57:54 GMT

←Older revision		Revision as of 15:57, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4f61]] is a 9 chain structure with sequence from [http://en.wikipedia.org/wiki/Ovis_aries Ovis aries] and [http://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F61 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4F61 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4f61]] is a 9 chain structure with sequence from [http://en.wikipedia.org/wiki/Ovis_aries Ovis aries] and [http://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F61 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4F61 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4f6r\|4f6r]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4f6r\|4f6r]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4f61 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f61 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4f61 RCSB], [http://www.ebi.ac.uk/pdbsum/4f61 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4f61 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f61 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4f61 RCSB], [http://www.ebi.ac.uk/pdbsum/4f61 PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/D0VWZ0_SHEEP D0VWZ0_SHEEP]] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain (By similarity).[RuleBase:RU003505][SAAS:SAAS023123_004_019801] [[http://www.uniprot.org/uniprot/D0VWY9_SHEEP D0VWY9_SHEEP]] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain (By similarity).[RuleBase:RU003505]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 26:
	[[Category: Ovis aries]]		[[Category: Ovis aries]]
	[[Category: Synthetic construct]]		[[Category: Synthetic construct]]
-	[[Category: Gigant, B.]]	+	[[Category: Gigant, B]]
-	[[Category: Knossow, M.]]	+	[[Category: Knossow, M]]
-	[[Category: Mignot, I.]]	+	[[Category: Mignot, I]]
	[[Category: Alpha-tubulin]]		[[Category: Alpha-tubulin]]
	[[Category: Beta-tubulin]]		[[Category: Beta-tubulin]]

OCA at 09:07, 2 July 2014

OCA — Wed, 02 Jul 2014 09:07:50 GMT

←Older revision		Revision as of 09:07, 2 July 2014
Line 1:		Line 1:
-	[[~~Image:~~4f61.~~png~~\|~~left~~\|~~200px~~]]	+	==Tubulin:Stathmin-like domain complex==
		+	<StructureSection load='4f61' size='340' side='right' caption='[[4f61]], [[Resolution\|resolution]] 4.17Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4f61]] is a 9 chain structure with sequence from [http://en.wikipedia.org/wiki/Ovis_aries Ovis aries] and [http://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F61 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4F61 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4f6r\|4f6r]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4f61 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f61 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4f61 RCSB], [http://www.ebi.ac.uk/pdbsum/4f61 PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	In cells, microtubule dynamics is regulated by stabilizing and destabilizing factors. Whereas proteins in both categories have been identified, their mechanism of action is rarely understood at the molecular level. This is due in part to the difficulties faced in structural approaches to obtain atomic models when tubulin is involved. Here, we design and characterize new stathmin-like domain (SLD) proteins that sequester tubulins in numbers different from two, the number of tubulins bound by stathmin or by the SLD of RB3, two stathmin family members that have been extensively studied. We established rules for the design of tight tubulin-SLD assemblies and applied them to complexes containing one to four tubulin heterodimers. Biochemical and structural experiments showed that the engineered SLDs behaved as expected. The new SLDs will be tools for structural studies of microtubule regulation. The larger complexes will be useful for cryo-electron microscopy, whereas crystallography or nuclear magnetic resonance will benefit from the 1:1 tubulin-SLD assembly. Finally, our results provide new insight into SLD function, suggesting that a major effect of these phosphorylatable proteins is the programmed release of sequestered tubulin for microtubule assembly at the specific cellular locations of members of the stathmin family.

-	~~{{STRUCTURE_4f61\| PDB=4f61 \| SCENE= }}~~	+	Design and characterization of modular scaffolds for tubulin assembly.,Mignot I, Pecqueur L, Dorleans A, Karuppasamy M, Ravelli RB, Dreier B, Pluckthun A, Knossow M, Gigant B J Biol Chem. 2012 Sep 7;287(37):31085-94. Epub 2012 Jul 12. PMID:22791712<ref>PMID:22791712</ref>

-	~~===Tubulin:Stathmin-like domain complex===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

-	~~{{ABSTRACT_PUBMED_22791712}}~~	+	==See Also==
-		+	*[[Tubulin\|Tubulin]]
-	==~~About this Structure~~==	+	== References ==
-	[[~~4f61~~]] ~~is a 9 chain structure with sequence from [http:~~//en.wikipedia.org/wiki/Ovis_aries Ovis aries] and [http://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F61 OCA].	+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Ovis aries]]		[[Category: Ovis aries]]
	[[Category: Synthetic construct]]		[[Category: Synthetic construct]]

OCA at 20:31, 26 September 2012

OCA — Wed, 26 Sep 2012 20:31:16 GMT

←Older revision		Revision as of 20:31, 26 September 2012
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-	[[Image:4f61.~~jpg~~\|left\|200px]]	+	[[Image:4f61.png\|left\|200px]]

	{{STRUCTURE_4f61\| PDB=4f61 \| SCENE= }}		{{STRUCTURE_4f61\| PDB=4f61 \| SCENE= }}
Line 5:		Line 5:
	===Tubulin:Stathmin-like domain complex===		===Tubulin:Stathmin-like domain complex===

		+	{{ABSTRACT_PUBMED_22791712}}

	==About this Structure==		==About this Structure==

OCA at 05:30, 18 July 2012

OCA — Wed, 18 Jul 2012 05:30:51 GMT

←Older revision		Revision as of 05:30, 18 July 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:4f61.jpg\|left\|200px]]

-	~~The entry~~ 4f61 ~~is ON HOLD~~	+	{{STRUCTURE_4f61\| PDB=4f61 \| SCENE= }}

-	~~Authors~~: ~~Gigant, B., Mignot, I., Knossow, M.~~	+	===Tubulin:Stathmin-like domain complex===

-	~~Description~~: ~~Tubulin~~:~~Stathmin~~-~~like domain complex~~	+
		+	==About this Structure==
		+	[[4f61]] is a 9 chain structure with sequence from [http://en.wikipedia.org/wiki/Ovis_aries Ovis aries] and [http://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F61 OCA].
		+	[[Category: Ovis aries]]
		+	[[Category: Synthetic construct]]
		+	[[Category: Gigant, B.]]
		+	[[Category: Knossow, M.]]
		+	[[Category: Mignot, I.]]
		+	[[Category: Alpha-tubulin]]
		+	[[Category: Beta-tubulin]]
		+	[[Category: Cell cycle]]
		+	[[Category: Gtpase]]
		+	[[Category: Microtubule]]
		+	[[Category: Rb3]]
		+	[[Category: Stathmin tubulin]]

OCA at 05:31, 30 May 2012

OCA — Wed, 30 May 2012 05:31:32 GMT

←Older revision		Revision as of 05:31, 30 May 2012
Line 5:		Line 5:
	Authors: Gigant, B., Mignot, I., Knossow, M.		Authors: Gigant, B., Mignot, I., Knossow, M.

-	Description: ~~TUBULIN~~:~~STATHMIN~~-~~LIKE DOMAIN COMPLEX~~	+	Description: Tubulin:Stathmin-like domain complex

OCA: Protected "4f61" [edit=sysop:move=sysop]

OCA — Wed, 23 May 2012 05:43:17 GMT

Protected "4f61" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:43, 23 May 2012