4f7g - Revision history

OCA at 11:11, 1 March 2024

OCA — Fri, 01 Mar 2024 11:11:28 GMT

←Older revision		Revision as of 11:11, 1 March 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4f7g]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F7G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4F7G FirstGlance]. <br>		<table><tr><td colspan='2'>[[4f7g]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F7G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4F7G FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4f7g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f7g OCA], [https://pdbe.org/4f7g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4f7g RCSB], [https://www.ebi.ac.uk/pdbsum/4f7g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4f7g ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.05Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4f7g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f7g OCA], [https://pdbe.org/4f7g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4f7g RCSB], [https://www.ebi.ac.uk/pdbsum/4f7g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4f7g ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/TLN1_MOUSE TLN1_MOUSE] Probably involved in connections of major cytoskeletal structures to the plasma membrane. High molecular weight cytoskeletal protein concentrated at regions of cell-substratum contact and, in lymphocytes, at cell-cell contacts.		[https://www.uniprot.org/uniprot/TLN1_MOUSE TLN1_MOUSE] Probably involved in connections of major cytoskeletal structures to the plasma membrane. High molecular weight cytoskeletal protein concentrated at regions of cell-substratum contact and, in lymphocytes, at cell-cell contacts.
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The activation of heterodimeric (alpha/beta) integrin transmembrane receptors by cytosolic protein talin is crucial for regulating diverse cell-adhesion-dependent processes, including blood coagulation, tissue remodeling, and cancer metastasis. This process is triggered by the coincident binding of N-terminal FERM (four-point-one-protein/ezrin/radixin/moesin) domain of talin (talin-FERM) to the inner membrane surface and integrin beta cytoplasmic tail, but how these binding events are spatiotemporally regulated remains obscure. Here we report the crystal structure of a dormant talin, revealing how a C-terminal talin rod segment (talin-RS) self-masks a key integrin-binding site on talin-FERM via a large interface. Unexpectedly, the structure also reveals a distinct negatively charged surface on talin-RS that electrostatically hinders the talin-FERM binding to the membrane. Such a dual inhibitory topology for talin is consistent with the biochemical and functional data, but differs significantly from a previous model. We show that upon enrichment with phosphotidylinositol-4,5-bisphosphate (PIP2) - a known talin activator, membrane strongly attracts a positively charged surface on talin-FERM and simultaneously repels the negatively charged surface on talin-RS. Such an electrostatic "pull-push" process promotes the relief of the dual inhibition of talin-FERM, which differs from the classic "steric clash" model for conventional PIP2-induced FERM domain activation. These data therefore unravel a new type of membrane-dependent FERM domain regulation and illustrate how it mediates the talin on/off switches to regulate integrin transmembrane signaling and cell adhesion.Cell Research advance online publication 19 June 2012; doi:10.1038/cr.2012.97.
-
-	A novel membrane-dependent on/off switch mechanism of talin FERM domain at sites of cell adhesion.,Song X, Yang J, Hirbawi J, Ye S, Perera HD, Goksoy E, Dwivedi P, Plow EF, Zhang R, Qin J Cell Res. 2012 Jun 19. doi: 10.1038/cr.2012.97. PMID:22710802<ref>PMID:22710802</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 4f7g" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
-	*[[Talin\|Talin]]	+	*[[Talin 3D structures\|Talin 3D structures]]
-	~~== References ==~~	+
-	~~<references/>~~	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 04:39, 7 October 2022

OCA — Fri, 07 Oct 2022 04:39:05 GMT

←Older revision		Revision as of 04:39, 7 October 2022
Line 1:		Line 1:

	==Crystal structure of talin autoinhibition complex==		==Crystal structure of talin autoinhibition complex==
-	<StructureSection load='4f7g' size='340' side='right' caption='[[4f7g]], [[Resolution\|resolution]] 2.05Å' scene=''>	+	<StructureSection load='4f7g' size='340' side='right'caption='[[4f7g]], [[Resolution\|resolution]] 2.05Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4f7g]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Lk3_transgenic_mice Lk3 transgenic mice~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F7G OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4F7G FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4f7g]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F7G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4F7G FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Tln1, Tln ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4f7g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f7g OCA], [https://pdbe.org/4f7g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4f7g RCSB], [https://www.ebi.ac.uk/pdbsum/4f7g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4f7g ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4f7g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f7g OCA], [~~http~~://pdbe.org/4f7g PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4f7g RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4f7g PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4f7g ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/TLN1_MOUSE TLN1_MOUSE]] Probably involved in connections of major cytoskeletal structures to the plasma membrane. High molecular weight cytoskeletal protein concentrated at regions of cell-substratum contact and, in lymphocytes, at cell-cell contacts.	+	[https://www.uniprot.org/uniprot/TLN1_MOUSE TLN1_MOUSE] Probably involved in connections of major cytoskeletal structures to the plasma membrane. High molecular weight cytoskeletal protein concentrated at regions of cell-substratum contact and, in lymphocytes, at cell-cell contacts.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 25:		Line 24:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Lk3 transgenic mice~~]]	+	[[Category: Large Structures]]
-	[[Category: Qin, J]]	+	[[Category: Mus musculus]]
-	[[Category: Song, X]]	+	[[Category: Qin J]]
-	[[Category: Ye, S]]	+	[[Category: Song X]]
-	[[Category: Zhang, R]]	+	[[Category: Ye S]]
-	~~[[Category: Alpha-helix bundle]]~~	+	[[Category: Zhang R]]
-	~~[[Category: Cell adhesion]]~~	+
-	~~[[Category: Integrin activation~~]]	+

OCA at 19:24, 4 August 2016

OCA — Thu, 04 Aug 2016 19:24:58 GMT

←Older revision		Revision as of 19:24, 4 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of talin autoinhibition complex==		==Crystal structure of talin autoinhibition complex==
	<StructureSection load='4f7g' size='340' side='right' caption='[[4f7g]], [[Resolution\|resolution]] 2.05Å' scene=''>		<StructureSection load='4f7g' size='340' side='right' caption='[[4f7g]], [[Resolution\|resolution]] 2.05Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4f7g]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Mus_musculus Mus musculus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F7G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4F7G FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4f7g]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F7G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4F7G FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Tln1, Tln ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 ~~Mus musculus~~])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Tln1, Tln ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4f7g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f7g OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4f7g RCSB], [http://www.ebi.ac.uk/pdbsum/4f7g PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4f7g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f7g OCA], [http://pdbe.org/4f7g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4f7g RCSB], [http://www.ebi.ac.uk/pdbsum/4f7g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4f7g ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4f7g" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 23:		Line 25:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Mus musculus~~]]	+	[[Category: Lk3 transgenic mice]]
	[[Category: Qin, J]]		[[Category: Qin, J]]
	[[Category: Song, X]]		[[Category: Song, X]]

OCA at 18:43, 24 December 2014

OCA — Wed, 24 Dec 2014 18:43:33 GMT

←Older revision		Revision as of 18:43, 24 December 2014
Line 6:		Line 6:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4f7g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f7g OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4f7g RCSB], [http://www.ebi.ac.uk/pdbsum/4f7g PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4f7g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f7g OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4f7g RCSB], [http://www.ebi.ac.uk/pdbsum/4f7g PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/TLN1_MOUSE TLN1_MOUSE]] Probably involved in connections of major cytoskeletal structures to the plasma membrane. High molecular weight cytoskeletal protein concentrated at regions of cell-substratum contact and, in lymphocytes, at cell-cell contacts.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 15:14, 9 December 2014

OCA — Tue, 09 Dec 2014 15:14:48 GMT

←Older revision		Revision as of 15:14, 9 December 2014
Line 1:		Line 1:
-	[[~~Image:~~4f7g.~~png~~\|~~left\|200px~~]]	+	==Crystal structure of talin autoinhibition complex==
		+	<StructureSection load='4f7g' size='340' side='right' caption='[[4f7g]], [[Resolution\|resolution]] 2.05Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4f7g]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F7G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4F7G FirstGlance]. <br>
		+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Tln1, Tln ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4f7g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f7g OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4f7g RCSB], [http://www.ebi.ac.uk/pdbsum/4f7g PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The activation of heterodimeric (alpha/beta) integrin transmembrane receptors by cytosolic protein talin is crucial for regulating diverse cell-adhesion-dependent processes, including blood coagulation, tissue remodeling, and cancer metastasis. This process is triggered by the coincident binding of N-terminal FERM (four-point-one-protein/ezrin/radixin/moesin) domain of talin (talin-FERM) to the inner membrane surface and integrin beta cytoplasmic tail, but how these binding events are spatiotemporally regulated remains obscure. Here we report the crystal structure of a dormant talin, revealing how a C-terminal talin rod segment (talin-RS) self-masks a key integrin-binding site on talin-FERM via a large interface. Unexpectedly, the structure also reveals a distinct negatively charged surface on talin-RS that electrostatically hinders the talin-FERM binding to the membrane. Such a dual inhibitory topology for talin is consistent with the biochemical and functional data, but differs significantly from a previous model. We show that upon enrichment with phosphotidylinositol-4,5-bisphosphate (PIP2) - a known talin activator, membrane strongly attracts a positively charged surface on talin-FERM and simultaneously repels the negatively charged surface on talin-RS. Such an electrostatic "pull-push" process promotes the relief of the dual inhibition of talin-FERM, which differs from the classic "steric clash" model for conventional PIP2-induced FERM domain activation. These data therefore unravel a new type of membrane-dependent FERM domain regulation and illustrate how it mediates the talin on/off switches to regulate integrin transmembrane signaling and cell adhesion.Cell Research advance online publication 19 June 2012; doi:10.1038/cr.2012.97.

-	~~{{STRUCTURE_4f7g\| PDB=4f7g \| SCENE= }}~~	+	A novel membrane-dependent on/off switch mechanism of talin FERM domain at sites of cell adhesion.,Song X, Yang J, Hirbawi J, Ye S, Perera HD, Goksoy E, Dwivedi P, Plow EF, Zhang R, Qin J Cell Res. 2012 Jun 19. doi: 10.1038/cr.2012.97. PMID:22710802<ref>PMID:22710802</ref>

-	~~===Crystal structure~~ of ~~talin autoinhibition complex===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

-	~~{{ABSTRACT_PUBMED_22710802}}~~	+	==See Also==
-		+	*[[Talin\|Talin]]
-	==~~About this Structure~~==	+	== References ==
-	[[~~4f7g~~]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F7G OCA].	+	<references/>
-		+	__TOC__
-	==~~Reference~~==	+	</StructureSection>
-	<~~ref group="xtra">PMID:022710802<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
-	[[Category: Qin, J.]]	+	[[Category: Qin, J]]
-	[[Category: Song, X.]]	+	[[Category: Song, X]]
-	[[Category: Ye, S.]]	+	[[Category: Ye, S]]
-	[[Category: Zhang, R.]]	+	[[Category: Zhang, R]]
	[[Category: Alpha-helix bundle]]		[[Category: Alpha-helix bundle]]
	[[Category: Cell adhesion]]		[[Category: Cell adhesion]]
	[[Category: Integrin activation]]		[[Category: Integrin activation]]

OCA at 09:55, 21 November 2012

OCA — Wed, 21 Nov 2012 09:55:57 GMT

←Older revision		Revision as of 09:55, 21 November 2012
Line 1:		Line 1:
-	[[Image:4f7g.~~jpg~~\|left\|200px]]	+	[[Image:4f7g.png\|left\|200px]]

	{{STRUCTURE_4f7g\| PDB=4f7g \| SCENE= }}		{{STRUCTURE_4f7g\| PDB=4f7g \| SCENE= }}

OCA at 07:39, 4 July 2012

OCA — Wed, 04 Jul 2012 07:39:22 GMT

←Older revision		Revision as of 07:39, 4 July 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:4f7g.jpg\|left\|200px]]

-	~~The entry~~ 4f7g ~~is ON HOLD~~	+	{{STRUCTURE_4f7g\| PDB=4f7g \| SCENE= }}

-	~~Authors: Song, X., Qin, J., Ye, S., Zhang, R.~~	+	===Crystal structure of talin autoinhibition complex===

-	~~Description: Crystal~~ structure ~~of talin autoinhibition complex~~	+	{{ABSTRACT_PUBMED_22710802}}
		+
		+	==About this Structure==
		+	[[4f7g]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F7G OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:022710802</ref><references group="xtra"/>
		+	[[Category: Mus musculus]]
		+	[[Category: Qin, J.]]
		+	[[Category: Song, X.]]
		+	[[Category: Ye, S.]]
		+	[[Category: Zhang, R.]]
		+	[[Category: Alpha-helix bundle]]
		+	[[Category: Cell adhesion]]
		+	[[Category: Integrin activation]]

OCA: Protected "4f7g" [edit=sysop:move=sysop]

OCA — Wed, 23 May 2012 05:44:46 GMT

Protected "4f7g" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:44, 23 May 2012

OCA: New page: '''Unreleased structure''' The entry 4f7g is ON HOLD Authors: Song, X., Qin, J., Ye, S., Zhang, R. Description: Crystal structure of talin autoinhibition complex

OCA — Wed, 23 May 2012 05:44:45 GMT

New page: '''Unreleased structure''' The entry 4f7g is ON HOLD Authors: Song, X., Qin, J., Ye, S., Zhang, R. Description: Crystal structure of talin autoinhibition complex

New page

'''Unreleased structure'''

The entry 4f7g is ON HOLD

Authors: Song, X., Qin, J., Ye, S., Zhang, R.

Description: Crystal structure of talin autoinhibition complex