4fak - Revision history

OCA at 15:20, 14 March 2024

OCA — Thu, 14 Mar 2024 15:20:04 GMT

←Older revision		Revision as of 15:20, 14 March 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4fak]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FAK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4FAK FirstGlance]. <br>		<table><tr><td colspan='2'>[[4fak]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FAK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4FAK FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.7Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4fak FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fak OCA], [https://pdbe.org/4fak PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4fak RCSB], [https://www.ebi.ac.uk/pdbsum/4fak PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4fak ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4fak FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fak OCA], [https://pdbe.org/4fak PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4fak RCSB], [https://www.ebi.ac.uk/pdbsum/4fak PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4fak ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/RLMH_STAAU RLMH_STAAU] Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA.[HAMAP-Rule:MF_00658]		[https://www.uniprot.org/uniprot/RLMH_STAAU RLMH_STAAU] Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA.[HAMAP-Rule:MF_00658]
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The gene orfX is conserved among all staphylococci, and its complete sequence is maintained upon insertion of the staphylococcal chromosome cassette mec (SCCmec) genomic island, containing the gene encoding resistance to beta-lactam antibiotics (mecA), into its C terminus. The function of OrfX has not been determined. We show that OrfX was constitutively produced during growth, that orfX could be inactivated without altering bacterial growth, and that insertion of SCCmec did not alter gene expression. We solved the crystal structure of OrfX at 1.7 A and found that it belongs to the S-adenosyl-l-methionine (AdoMet)-dependent alpha/beta-knot superfamily of SPOUT methyltransferases (MTases), with a high structural homology to YbeA, the gene product of the Escherichia coli 70 S ribosomal MTase RlmH. MTase activity was confirmed by demonstrating the OrfX-dependent methylation of the Staphylococcus aureus 70 S ribosome. When OrfX was crystallized in the presence of its AdoMet substrate, we found that each monomer of the homodimeric structure bound AdoMet in its active site. Solution studies using isothermal titration calorimetry confirmed that each monomer bound AdoMet but with different binding affinities (K(d) = 52 +/- 0.4 and 606 +/- 2 mum). In addition, the structure shows that the AdoMet-binding pocket, formed by a deep trefoil knot, contains a bound phosphate molecule, which is the likely nucleotide methylation site. This study represents the first characterization of a staphylococcal ribosomal MTase and provides the first crystal structure of a member of the alpha/beta-knot superfamily of SPOUT MTases in the RlmH or COG1576 family with bound AdoMet.
-
-	Characterization of the Staphylococcus aureus rRNA Methyltransferase Encoded by orfX, the Gene Containing the Staphylococcal Chromosome Cassette mec (SCCmec) Insertion Site.,Boundy S, Safo MK, Wang L, Musayev FN, O'Farrell HC, Rife JP, Archer GL J Biol Chem. 2013 Jan 4;288(1):132-40. doi: 10.1074/jbc.M112.385138. Epub 2012, Nov 13. PMID:23150671<ref>PMID:23150671</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 4fak" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 04:45, 7 October 2022

OCA — Fri, 07 Oct 2022 04:45:13 GMT

←Older revision		Revision as of 04:45, 7 October 2022
Line 1:		Line 1:

	==Crystal Structure of OrfX in Complex with S-Adenosylmethionine==		==Crystal Structure of OrfX in Complex with S-Adenosylmethionine==
-	<StructureSection load='4fak' size='340' side='right' caption='[[4fak]], [[Resolution\|resolution]] 1.70Å' scene=''>	+	<StructureSection load='4fak' size='340' side='right'caption='[[4fak]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4fak]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus~~ aureus~~" (rosenbach 1884) zopf 1885~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FAK OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4FAK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4fak]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FAK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4FAK FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">orfx, rlmH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 "Micrococcus aureus" (Rosenbach 1884) Zopf 1885])</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4fak FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fak OCA], [https://pdbe.org/4fak PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4fak RCSB], [https://www.ebi.ac.uk/pdbsum/4fak PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4fak ProSAT]</span></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/23S_rRNA_(pseudouridine(1915)-N(3))-methyltransferase 23S rRNA (pseudouridine(1915)-N(3))-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.177 2.1.1.177] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4fak FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fak OCA], [~~http~~://pdbe.org/4fak PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4fak RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4fak PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4fak ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/RLMH_STAAU RLMH_STAAU]] Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA.[HAMAP-Rule:MF_00658]	+	[https://www.uniprot.org/uniprot/RLMH_STAAU RLMH_STAAU] Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA.[HAMAP-Rule:MF_00658]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Archer, G L~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Boundy, S~~]]	+	[[Category: Staphylococcus aureus]]
-	[[Category: ~~Farrell, H C.O~~]]	+	[[Category: Archer GL]]
-	[[Category: ~~Musayev, F N~~]]	+	[[Category: Boundy S]]
-	[[Category: ~~Rife, J P~~]]	+	[[Category: Musayev FN]]
-	[[Category: ~~Safo, M K~~]]	+	[[Category: O'Farrell HC]]
-	[[Category: ~~Adomet~~]]	+	[[Category: Rife JP]]
-	[[Category: ~~Alpha/beta methyltransferase rossmann fold]]~~	+	[[Category: Safo MK]]
-	~~[[Category: Ribosomal protein]]~~	+
-	~~[[Category: Rrna]]~~	+
-	~~[[Category: Rrna methylation]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 13:34, 5 August 2016

OCA — Fri, 05 Aug 2016 13:34:55 GMT

←Older revision		Revision as of 13:34, 5 August 2016
Line 1:		Line 1:
		+
	==Crystal Structure of OrfX in Complex with S-Adenosylmethionine==		==Crystal Structure of OrfX in Complex with S-Adenosylmethionine==
	<StructureSection load='4fak' size='340' side='right' caption='[[4fak]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='4fak' size='340' side='right' caption='[[4fak]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4fak]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Staphylococcus_aureus Staphylococcus~~ aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FAK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4FAK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4fak]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FAK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4FAK FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">orfx, rlmH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 ~~Staphylococcus~~ aureus])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">orfx, rlmH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 "Micrococcus aureus" (Rosenbach 1884) Zopf 1885])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/23S_rRNA_(pseudouridine(1915)-N(3))-methyltransferase 23S rRNA (pseudouridine(1915)-N(3))-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.177 2.1.1.177] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/23S_rRNA_(pseudouridine(1915)-N(3))-methyltransferase 23S rRNA (pseudouridine(1915)-N(3))-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.177 2.1.1.177] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fak FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fak OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4fak RCSB], [http://www.ebi.ac.uk/pdbsum/4fak PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fak FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fak OCA], [http://pdbe.org/4fak PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4fak RCSB], [http://www.ebi.ac.uk/pdbsum/4fak PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4fak ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4fak" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Staphylococcus aureus]]
	[[Category: Archer, G L]]		[[Category: Archer, G L]]
	[[Category: Boundy, S]]		[[Category: Boundy, S]]

OCA at 02:55, 25 December 2014

OCA — Thu, 25 Dec 2014 02:55:08 GMT

←Older revision		Revision as of 02:55, 25 December 2014
Line 8:		Line 8:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fak FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fak OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4fak RCSB], [http://www.ebi.ac.uk/pdbsum/4fak PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fak FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fak OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4fak RCSB], [http://www.ebi.ac.uk/pdbsum/4fak PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/RLMH_STAAU RLMH_STAAU]] Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA.[HAMAP-Rule:MF_00658]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 15:26, 9 December 2014

OCA — Tue, 09 Dec 2014 15:26:58 GMT

←Older revision		Revision as of 15:26, 9 December 2014
Line 1:		Line 1:
-	[[~~Image:~~4fak.~~png~~\|~~left~~\|~~200px~~]]	+	==Crystal Structure of OrfX in Complex with S-Adenosylmethionine==
		+	<StructureSection load='4fak' size='340' side='right' caption='[[4fak]], [[Resolution\|resolution]] 1.70Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4fak]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FAK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4FAK FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">orfx, rlmH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 Staphylococcus aureus])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/23S_rRNA_(pseudouridine(1915)-N(3))-methyltransferase 23S rRNA (pseudouridine(1915)-N(3))-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.177 2.1.1.177] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fak FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fak OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4fak RCSB], [http://www.ebi.ac.uk/pdbsum/4fak PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The gene orfX is conserved among all staphylococci, and its complete sequence is maintained upon insertion of the staphylococcal chromosome cassette mec (SCCmec) genomic island, containing the gene encoding resistance to beta-lactam antibiotics (mecA), into its C terminus. The function of OrfX has not been determined. We show that OrfX was constitutively produced during growth, that orfX could be inactivated without altering bacterial growth, and that insertion of SCCmec did not alter gene expression. We solved the crystal structure of OrfX at 1.7 A and found that it belongs to the S-adenosyl-l-methionine (AdoMet)-dependent alpha/beta-knot superfamily of SPOUT methyltransferases (MTases), with a high structural homology to YbeA, the gene product of the Escherichia coli 70 S ribosomal MTase RlmH. MTase activity was confirmed by demonstrating the OrfX-dependent methylation of the Staphylococcus aureus 70 S ribosome. When OrfX was crystallized in the presence of its AdoMet substrate, we found that each monomer of the homodimeric structure bound AdoMet in its active site. Solution studies using isothermal titration calorimetry confirmed that each monomer bound AdoMet but with different binding affinities (K(d) = 52 +/- 0.4 and 606 +/- 2 mum). In addition, the structure shows that the AdoMet-binding pocket, formed by a deep trefoil knot, contains a bound phosphate molecule, which is the likely nucleotide methylation site. This study represents the first characterization of a staphylococcal ribosomal MTase and provides the first crystal structure of a member of the alpha/beta-knot superfamily of SPOUT MTases in the RlmH or COG1576 family with bound AdoMet.

-	~~{{STRUCTURE_4fak\| PDB=4fak \| SCENE= }}~~	+	Characterization of the Staphylococcus aureus rRNA Methyltransferase Encoded by orfX, the Gene Containing the Staphylococcal Chromosome Cassette mec (SCCmec) Insertion Site.,Boundy S, Safo MK, Wang L, Musayev FN, O'Farrell HC, Rife JP, Archer GL J Biol Chem. 2013 Jan 4;288(1):132-40. doi: 10.1074/jbc.M112.385138. Epub 2012, Nov 13. PMID:23150671<ref>PMID:23150671</ref>

-	~~===Crystal Structure~~ of ~~OrfX in Complex with~~ S~~-Adenosylmethionine===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_23150671}}~~	+	== References ==
-		+	<references/>
-	==~~About this Structure~~==	+	__TOC__
-	~~[[4fak]] is a 1 chain structure with sequence from [http:~~//~~en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FAK OCA].~~	+	</StructureSection>
	[[Category: Staphylococcus aureus]]		[[Category: Staphylococcus aureus]]
-	[[Category: Archer, G L.]]	+	[[Category: Archer, G L]]
-	[[Category: Boundy, S.]]	+	[[Category: Boundy, S]]
-	[[Category: Farrell, H C.O.]]	+	[[Category: Farrell, H C.O]]
-	[[Category: Musayev, F N.]]	+	[[Category: Musayev, F N]]
-	[[Category: Rife, J P.]]	+	[[Category: Rife, J P]]
-	[[Category: Safo, M K.]]	+	[[Category: Safo, M K]]
	[[Category: Adomet]]		[[Category: Adomet]]
	[[Category: Alpha/beta methyltransferase rossmann fold]]		[[Category: Alpha/beta methyltransferase rossmann fold]]

OCA at 12:34, 30 January 2013

OCA — Wed, 30 Jan 2013 12:34:11 GMT

←Older revision		Revision as of 12:34, 30 January 2013
Line 1:		Line 1:
-	[[Image:4fak.~~jpg~~\|left\|200px]]	+	[[Image:4fak.png\|left\|200px]]

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-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_4fak\| PDB=4fak \| SCENE= }}		{{STRUCTURE_4fak\| PDB=4fak \| SCENE= }}

	===Crystal Structure of OrfX in Complex with S-Adenosylmethionine===		===Crystal Structure of OrfX in Complex with S-Adenosylmethionine===

		+	{{ABSTRACT_PUBMED_23150671}}

	==About this Structure==		==About this Structure==

OCA at 07:58, 6 June 2012

OCA — Wed, 06 Jun 2012 07:58:19 GMT

←Older revision		Revision as of 07:58, 6 June 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:4fak.jpg\|left\|200px]]

-	The ~~entry 4fak~~ is ~~ON HOLD~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_4fak", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_4fak\| PDB=4fak \| SCENE= }}

-	~~Authors: Safo, M.K., Musayev, F. N., Boundy,~~ S~~., Archer, G. L., Rife, J. P., O'Farrell, H.C~~	+	===Crystal Structure of OrfX in Complex with S-Adenosylmethionine===

-	~~Description: Crystal~~ Structure ~~of OrfX in Complex~~ with S-~~Adenosylmethionine~~	+
		+	==About this Structure==
		+	[[4fak]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FAK OCA].
		+	[[Category: Staphylococcus aureus]]
		+	[[Category: Archer, G L.]]
		+	[[Category: Boundy, S.]]
		+	[[Category: Farrell, H C.O.]]
		+	[[Category: Musayev, F N.]]
		+	[[Category: Rife, J P.]]
		+	[[Category: Safo, M K.]]
		+	[[Category: Adomet]]
		+	[[Category: Alpha/beta methyltransferase rossmann fold]]
		+	[[Category: Ribosomal protein]]
		+	[[Category: Rrna]]
		+	[[Category: Rrna methylation]]
		+	[[Category: Transferase]]

OCA: Protected "4fak" [edit=sysop:move=sysop]

OCA — Wed, 30 May 2012 05:33:52 GMT

Protected "4fak" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:33, 30 May 2012

OCA: New page: '''Unreleased structure''' The entry 4fak is ON HOLD Authors: Safo, M.K., Musayev, F. N., Boundy, S., Archer, G. L., Rife, J. P., O'Farrell, H.C Description: Crystal Structure of OrfX ...

OCA — Wed, 30 May 2012 05:33:51 GMT

New page: '''Unreleased structure''' The entry 4fak is ON HOLD Authors: Safo, M.K., Musayev, F. N., Boundy, S., Archer, G. L., Rife, J. P., O'Farrell, H.C Description: Crystal Structure of OrfX ...

New page

'''Unreleased structure'''

The entry 4fak is ON HOLD

Authors: Safo, M.K., Musayev, F. N., Boundy, S., Archer, G. L., Rife, J. P., O'Farrell, H.C

Description: Crystal Structure of OrfX in Complex with S-Adenosylmethionine