4fg4 - Revision history

OCA at 11:16, 1 March 2024

OCA — Fri, 01 Mar 2024 11:16:19 GMT

←Older revision		Revision as of 11:16, 1 March 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4fg4]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis_str._168 Bacillus subtilis subsp. subtilis str. 168]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FG4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4FG4 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4fg4]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis_str._168 Bacillus subtilis subsp. subtilis str. 168]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FG4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4FG4 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=GS1:1-THIO-BETA-D-GLUCOPYRANOSE'>GS1</scene>, <scene name='pdbligand=PRD_900095:hemithiocellodextrin'>PRD_900095</scene>, <scene name='pdbligand=SGC:4-DEOXY-4-THIO-BETA-D-GLUCOPYRANOSE'>SGC</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.701Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=GS1:1-THIO-BETA-D-GLUCOPYRANOSE'>GS1</scene>, <scene name='pdbligand=PRD_900095:hemithiocellodextrin'>PRD_900095</scene>, <scene name='pdbligand=SGC:4-DEOXY-4-THIO-BETA-D-GLUCOPYRANOSE'>SGC</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4fg4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fg4 OCA], [https://pdbe.org/4fg4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4fg4 RCSB], [https://www.ebi.ac.uk/pdbsum/4fg4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4fg4 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4fg4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fg4 OCA], [https://pdbe.org/4fg4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4fg4 RCSB], [https://www.ebi.ac.uk/pdbsum/4fg4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4fg4 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/YOAJ_BACSU YOAJ_BACSU] May promote colonization of plant roots. May cause loosening and extension of plant cell walls by disrupting non-covalent bonding between cellulose microfibrils and matrix glucans. Has very low expansin activity (in vitro). No enzymatic activity has been found. Binds to peptidoglycan and to plant cell walls.<ref>PMID:18971341</ref>		[https://www.uniprot.org/uniprot/YOAJ_BACSU YOAJ_BACSU] May promote colonization of plant roots. May cause loosening and extension of plant cell walls by disrupting non-covalent bonding between cellulose microfibrils and matrix glucans. Has very low expansin activity (in vitro). No enzymatic activity has been found. Binds to peptidoglycan and to plant cell walls.<ref>PMID:18971341</ref>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Components of modular cellulases, type-A cellulose-binding modules (CBMs) bind to crystalline cellulose and enhance enzyme effectiveness, but structural details of the interaction are uncertain. We analyzed cellulose binding by EXLX1, a bacterial expansin with ability to loosen plant cell walls and whose domain D2 has type-A CBM characteristics. EXLX1 strongly binds to crystalline cellulose via D2, whereas its affinity for soluble cellooligosaccharides is weak. Calorimetry indicated cellulose binding was largely entropically driven. We solved the crystal structures of EXLX1 complexed with cellulose-like oligosaccharides to find that EXLX1 binds the ligands through hydrophobic interactions of three linearly arranged aromatic residues in D2. The crystal structures revealed a unique form of ligand-mediated dimerization, with the oligosaccharide sandwiched between two D2 domains in opposite polarity. This report clarifies the molecular target of expansin and the specific molecular interactions of a type-A CBM with cellulose.
-
-	Structural basis for entropy-driven cellulose binding by a type-A cellulose-binding module (CBM) and bacterial expansin.,Georgelis N, Yennawar NH, Cosgrove DJ Proc Natl Acad Sci U S A. 2012 Sep 11;109(37):14830-5. Epub 2012 Aug 27. PMID:22927418<ref>PMID:22927418</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 4fg4" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 04:55, 7 October 2022

OCA — Fri, 07 Oct 2022 04:55:20 GMT

←Older revision		Revision as of 04:55, 7 October 2022
Line 1:		Line 1:

	==Crystal structure of Bacillus Subtilis expansin (EXLX1) in complex with hemithiocellodextrin==		==Crystal structure of Bacillus Subtilis expansin (EXLX1) in complex with hemithiocellodextrin==
-	<StructureSection load='4fg4' size='340' side='right' caption='[[4fg4]], [[Resolution\|resolution]] 2.70Å' scene=''>	+	<StructureSection load='4fg4' size='340' side='right'caption='[[4fg4]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4fg4]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Bacsu Bacsu~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FG4 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4FG4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4fg4]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis_str._168 Bacillus subtilis subsp. subtilis str. 168]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FG4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4FG4 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=~~0TS~~:BETA-D-~~GLUCOPYRANOSYL-(1- 4)-4-THIO-BETA-D-GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSYL-(1- 4)-4-THIO-BETA-D-GLUCOPYRANOSYL-(1- 4)-~~1-THIO-BETA-D-GLUCOPYRANOSE'>~~0TS~~</scene><~~/td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=GS1:1-THIO-BETA-D-GLUCOPYRANOSE'>GS1</scene>, <scene name='pdbligand=PRD_900095:hemithiocellodextrin'>PRD_900095</scene>, <scene name='pdbligand=SGC:4-DEOXY-4-THIO-BETA-D-GLUCOPYRANOSE'>SGC</scene></td></tr>
-	~~<tr id~~='~~related'><td class~~=~~"sblockLbl"><b>[[Related_structure\|Related~~:~~]]</b~~></td>~~<td class="sblockDat">[[4fer\|4fer]]~~, ~~[[4fft\|4fft]], [[4fg2\|4fg2]], [[3d30\|3d30]], [[2bh0\|2bh0]]~~<~~/td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4fg4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fg4 OCA], [https://pdbe.org/4fg4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4fg4 RCSB], [https://www.ebi.ac.uk/pdbsum/4fg4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4fg4 ProSAT]</span></td></tr>
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">yoaJ, BSU18630 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224308 BACSU])~~</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4fg4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fg4 OCA], [~~http~~://pdbe.org/4fg4 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4fg4 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4fg4 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4fg4 ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/YOAJ_BACSU YOAJ_BACSU]] May promote colonization of plant roots. May cause loosening and extension of plant cell walls by disrupting non-covalent bonding between cellulose microfibrils and matrix glucans. Has very low expansin activity (in vitro). No enzymatic activity has been found. Binds to peptidoglycan and to plant cell walls.<ref>PMID:18971341</ref>	+	[https://www.uniprot.org/uniprot/YOAJ_BACSU YOAJ_BACSU] May promote colonization of plant roots. May cause loosening and extension of plant cell walls by disrupting non-covalent bonding between cellulose microfibrils and matrix glucans. Has very low expansin activity (in vitro). No enzymatic activity has been found. Binds to peptidoglycan and to plant cell walls.<ref>PMID:18971341</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacsu~~]]	+	[[Category: Bacillus subtilis subsp. subtilis str. 168]]
-	[[Category: ~~Cosgrove, D J~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Georgelis, N~~]]	+	[[Category: Cosgrove DJ]]
-	[[Category: ~~Yennawar,~~ N H]]	+	[[Category: Georgelis N]]
-	[[Category: ~~Cellulose]]~~	+	[[Category: Yennawar NH]]
-	~~[[Category: Cellulose-binding protein~~]]	+

OCA at 14:24, 29 March 2017

OCA — Wed, 29 Mar 2017 14:24:23 GMT

←Older revision		Revision as of 14:24, 29 March 2017
Line 1:		Line 1:
		+
	==Crystal structure of Bacillus Subtilis expansin (EXLX1) in complex with hemithiocellodextrin==		==Crystal structure of Bacillus Subtilis expansin (EXLX1) in complex with hemithiocellodextrin==
	<StructureSection load='4fg4' size='340' side='right' caption='[[4fg4]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='4fg4' size='340' side='right' caption='[[4fg4]], [[Resolution\|resolution]] 2.70Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4fer\|4fer]], [[4fft\|4fft]], [[4fg2\|4fg2]], [[3d30\|3d30]], [[2bh0\|2bh0]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4fer\|4fer]], [[4fft\|4fft]], [[4fg2\|4fg2]], [[3d30\|3d30]], [[2bh0\|2bh0]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">yoaJ, BSU18630 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224308 BACSU])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">yoaJ, BSU18630 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224308 BACSU])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fg4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fg4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4fg4 RCSB], [http://www.ebi.ac.uk/pdbsum/4fg4 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fg4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fg4 OCA], [http://pdbe.org/4fg4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4fg4 RCSB], [http://www.ebi.ac.uk/pdbsum/4fg4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4fg4 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4fg4" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 06:53, 24 June 2015

OCA — Wed, 24 Jun 2015 06:53:48 GMT

←Older revision		Revision as of 06:53, 24 June 2015
Line 2:		Line 2:
	<StructureSection load='4fg4' size='340' side='right' caption='[[4fg4]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='4fg4' size='340' side='right' caption='[[4fg4]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4fg4]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Bacillus_subtilis_subsp._subtilis Bacillus subtilis subsp. subtilis~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FG4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4FG4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4fg4]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacsu Bacsu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FG4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4FG4 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=0TS:BETA-D-GLUCOPYRANOSYL-(1- 4)-4-THIO-BETA-D-GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSYL-(1- 4)-4-THIO-BETA-D-GLUCOPYRANOSYL-(1- 4)-1-THIO-BETA-D-GLUCOPYRANOSE'>0TS</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=0TS:BETA-D-GLUCOPYRANOSYL-(1- 4)-4-THIO-BETA-D-GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSYL-(1- 4)-4-THIO-BETA-D-GLUCOPYRANOSYL-(1- 4)-1-THIO-BETA-D-GLUCOPYRANOSE'>0TS</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4fer\|4fer]], [[4fft\|4fft]], [[4fg2\|4fg2]], [[3d30\|3d30]], [[2bh0\|2bh0]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4fer\|4fer]], [[4fft\|4fft]], [[4fg2\|4fg2]], [[3d30\|3d30]], [[2bh0\|2bh0]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">yoaJ, BSU18630 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=~~135461 Bacillus subtilis subsp. subtilis~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">yoaJ, BSU18630 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224308 BACSU])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fg4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fg4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4fg4 RCSB], [http://www.ebi.ac.uk/pdbsum/4fg4 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fg4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fg4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4fg4 RCSB], [http://www.ebi.ac.uk/pdbsum/4fg4 PDBsum]</span></td></tr>
	</table>		</table>
Line 22:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus subtilis subsp. subtilis~~]]	+	[[Category: Bacsu]]
	[[Category: Cosgrove, D J]]		[[Category: Cosgrove, D J]]
	[[Category: Georgelis, N]]		[[Category: Georgelis, N]]

OCA at 13:44, 25 December 2014

OCA — Thu, 25 Dec 2014 13:44:08 GMT

←Older revision		Revision as of 13:44, 25 December 2014
Line 1:		Line 1:
-	[[~~Image:~~4fg4.~~png~~\|~~left~~\|~~200px~~]]	+	==Crystal structure of Bacillus Subtilis expansin (EXLX1) in complex with hemithiocellodextrin==
		+	<StructureSection load='4fg4' size='340' side='right' caption='[[4fg4]], [[Resolution\|resolution]] 2.70Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4fg4]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis Bacillus subtilis subsp. subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FG4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4FG4 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=0TS:BETA-D-GLUCOPYRANOSYL-(1- 4)-4-THIO-BETA-D-GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSYL-(1- 4)-4-THIO-BETA-D-GLUCOPYRANOSYL-(1- 4)-1-THIO-BETA-D-GLUCOPYRANOSE'>0TS</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4fer\|4fer]], [[4fft\|4fft]], [[4fg2\|4fg2]], [[3d30\|3d30]], [[2bh0\|2bh0]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">yoaJ, BSU18630 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=135461 Bacillus subtilis subsp. subtilis])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fg4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fg4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4fg4 RCSB], [http://www.ebi.ac.uk/pdbsum/4fg4 PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/YOAJ_BACSU YOAJ_BACSU]] May promote colonization of plant roots. May cause loosening and extension of plant cell walls by disrupting non-covalent bonding between cellulose microfibrils and matrix glucans. Has very low expansin activity (in vitro). No enzymatic activity has been found. Binds to peptidoglycan and to plant cell walls.<ref>PMID:18971341</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Components of modular cellulases, type-A cellulose-binding modules (CBMs) bind to crystalline cellulose and enhance enzyme effectiveness, but structural details of the interaction are uncertain. We analyzed cellulose binding by EXLX1, a bacterial expansin with ability to loosen plant cell walls and whose domain D2 has type-A CBM characteristics. EXLX1 strongly binds to crystalline cellulose via D2, whereas its affinity for soluble cellooligosaccharides is weak. Calorimetry indicated cellulose binding was largely entropically driven. We solved the crystal structures of EXLX1 complexed with cellulose-like oligosaccharides to find that EXLX1 binds the ligands through hydrophobic interactions of three linearly arranged aromatic residues in D2. The crystal structures revealed a unique form of ligand-mediated dimerization, with the oligosaccharide sandwiched between two D2 domains in opposite polarity. This report clarifies the molecular target of expansin and the specific molecular interactions of a type-A CBM with cellulose.

-	~~{{STRUCTURE_4fg4\| PDB=4fg4 \| SCENE= }}~~	+	Structural basis for entropy-driven cellulose binding by a type-A cellulose-binding module (CBM) and bacterial expansin.,Georgelis N, Yennawar NH, Cosgrove DJ Proc Natl Acad Sci U S A. 2012 Sep 11;109(37):14830-5. Epub 2012 Aug 27. PMID:22927418<ref>PMID:22927418</ref>

-	~~===Crystal structure~~ of ~~Bacillus Subtilis expansin (EXLX1) in complex with hemithiocellodextrin===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_22927418}}~~	+	== References ==
-		+	<references/>
-	==~~About this Structure~~==	+	__TOC__
-	~~[[4fg4]] is a 2 chain structure with sequence from [http:~~//en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis Bacillus subtilis subsp. subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FG4 OCA].	+	</StructureSection>
	[[Category: Bacillus subtilis subsp. subtilis]]		[[Category: Bacillus subtilis subsp. subtilis]]
-	[[Category: Cosgrove, D J.]]	+	[[Category: Cosgrove, D J]]
-	[[Category: Georgelis, N.]]	+	[[Category: Georgelis, N]]
-	[[Category: Yennawar, N H.]]	+	[[Category: Yennawar, N H]]
	[[Category: Cellulose]]		[[Category: Cellulose]]
	[[Category: Cellulose-binding protein]]		[[Category: Cellulose-binding protein]]

OCA at 20:36, 26 September 2012

OCA — Wed, 26 Sep 2012 20:36:42 GMT

←Older revision		Revision as of 20:36, 26 September 2012
Line 1:		Line 1:
-	[[Image:4fg4.~~jpg~~\|left\|200px]]	+	[[Image:4fg4.png\|left\|200px]]

	{{STRUCTURE_4fg4\| PDB=4fg4 \| SCENE= }}		{{STRUCTURE_4fg4\| PDB=4fg4 \| SCENE= }}
Line 5:		Line 5:
	===Crystal structure of Bacillus Subtilis expansin (EXLX1) in complex with hemithiocellodextrin===		===Crystal structure of Bacillus Subtilis expansin (EXLX1) in complex with hemithiocellodextrin===

		+	{{ABSTRACT_PUBMED_22927418}}

	==About this Structure==		==About this Structure==

OCA at 06:06, 22 August 2012

OCA — Wed, 22 Aug 2012 06:06:49 GMT

←Older revision		Revision as of 06:06, 22 August 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:4fg4.jpg\|left\|200px]]

-	~~The entry~~ 4fg4 ~~is ON HOLD~~ ~~until Paper Publication~~	+	{{STRUCTURE_4fg4\| PDB=4fg4 \| SCENE= }}

-	~~Authors: Georgelis, N., Yennawar, N.H., Cosgrove, D.J.~~	+	===Crystal structure of Bacillus Subtilis expansin (EXLX1) in complex with hemithiocellodextrin===

-	~~Description: Crystal~~ structure ~~of Bacillus Subtilis expansin (EXLX1) in complex~~ with ~~hemithiocellodextrin~~	+
		+	==About this Structure==
		+	[[4fg4]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis Bacillus subtilis subsp. subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FG4 OCA].
		+	[[Category: Bacillus subtilis subsp. subtilis]]
		+	[[Category: Cosgrove, D J.]]
		+	[[Category: Georgelis, N.]]
		+	[[Category: Yennawar, N H.]]
		+	[[Category: Cellulose]]
		+	[[Category: Cellulose-binding protein]]

OCA at 05:24, 27 June 2012

OCA — Wed, 27 Jun 2012 05:24:02 GMT

←Older revision		Revision as of 05:24, 27 June 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4fg4 is ON HOLD	+	The entry 4fg4 is ON HOLD until Paper Publication

	Authors: Georgelis, N., Yennawar, N.H., Cosgrove, D.J.		Authors: Georgelis, N., Yennawar, N.H., Cosgrove, D.J.

	Description: Crystal structure of Bacillus Subtilis expansin (EXLX1) in complex with hemithiocellodextrin		Description: Crystal structure of Bacillus Subtilis expansin (EXLX1) in complex with hemithiocellodextrin

OCA at 07:25, 20 June 2012

OCA — Wed, 20 Jun 2012 07:25:15 GMT

←Older revision		Revision as of 07:25, 20 June 2012
Line 3:		Line 3:
	The entry 4fg4 is ON HOLD		The entry 4fg4 is ON HOLD

-	Authors: Georgelis, N., Yennawar, N. H., Cosgrove, D. J.	+	Authors: Georgelis, N., Yennawar, N.H., Cosgrove, D.J.

	Description: Crystal structure of Bacillus Subtilis expansin (EXLX1) in complex with hemithiocellodextrin		Description: Crystal structure of Bacillus Subtilis expansin (EXLX1) in complex with hemithiocellodextrin

OCA: Protected "4fg4" [edit=sysop:move=sysop]

OCA — Wed, 13 Jun 2012 05:51:23 GMT

Protected "4fg4" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:51, 13 June 2012