4fil - Revision history

OCA at 15:26, 14 March 2024

OCA — Thu, 14 Mar 2024 15:26:05 GMT

←Older revision		Revision as of 15:26, 14 March 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4fil]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FIL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4FIL FirstGlance]. <br>		<table><tr><td colspan='2'>[[4fil]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FIL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4FIL FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=0UE:FERRIOXAMINE+B'>0UE</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.4Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=0UE:FERRIOXAMINE+B'>0UE</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4fil FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fil OCA], [https://pdbe.org/4fil PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4fil RCSB], [https://www.ebi.ac.uk/pdbsum/4fil PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4fil ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4fil FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fil OCA], [https://pdbe.org/4fil PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4fil RCSB], [https://www.ebi.ac.uk/pdbsum/4fil PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4fil ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/A0A0H3JWU6_STAAM A0A0H3JWU6_STAAM]		[https://www.uniprot.org/uniprot/A0A0H3JWU6_STAAM A0A0H3JWU6_STAAM]
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Iron acquisition is a central process for virtually all organisms. In Staphylococcus aureus, FhuD2 is a lipoprotein that is a high-affinity receptor for iron-bound hydroxamate siderophores. In this study, FhuD2 was crystallized bound to ferrioxamine-B (FXB), and also in its ligand-free state; the latter structures are the first for hydroxamate-binding receptors within this protein family. The structure of the FhuD2-FXB conformation shows that residues W197 and R199 from the C-terminal domain donate hydrogen bonds to the hydroxamate oxygens, and a ring of aromatic residues cradles the aliphatic arms connecting the hydroxamate moieties of the siderophore. The available ligand-bound structures of FhuD from Escherichia coli and YfiY from Bacillus cereus show that, despite a high degree of structural conservation, three protein families have evolved with critical siderophore binding residues on either the C-terminal domain (S. aureus), the N-terminal domain (E. coli), or both (B. cereus). Unliganded FhuD2 was crystallized in five conformations related by rigid body movements of the N- and C-terminal domains. Small-angle X-ray scattering (SAXS) indicates that the solution conformation of unliganded FhuD2 is more compact than the conformations observed in crystals. The ligand-induced conformational changes for FhuD2 in solution are relatively modest and depend on the identity of the siderophore. The crystallographic and SAXS results are used to discuss roles for the liganded and unliganded forms of FhuD2 in the siderophore transport mechanism.
-
-	Crystal and solution structure analysis of FhuD2 from Staphylococcus aureus in multiple unliganded conformations and bound to ferrioxamine-B.,Podkowa KJ, Briere LA, Heinrichs DE, Shilton BH Biochemistry. 2014 Apr 1;53(12):2017-31. doi: 10.1021/bi401349d. Epub 2014 Mar, 19. PMID:24606332<ref>PMID:24606332</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 4fil" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Ferric hydroxamate uptake receptor\|Ferric hydroxamate uptake receptor]]		*[[Ferric hydroxamate uptake receptor\|Ferric hydroxamate uptake receptor]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 19:50, 19 October 2022

OCA — Wed, 19 Oct 2022 19:50:40 GMT

←Older revision		Revision as of 19:50, 19 October 2022
Line 1:		Line 1:

	==Structure of FhuD2 from Staphylococcus Aureus with Bound Ferrioxamine B==		==Structure of FhuD2 from Staphylococcus Aureus with Bound Ferrioxamine B==
-	<StructureSection load='4fil' size='340' side='right' caption='[[4fil]], [[Resolution\|resolution]] 2.40Å' scene=''>	+	<StructureSection load='4fil' size='340' side='right'caption='[[4fil]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4fil]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus~~ aureus~~" (rosenbach 1884) zopf 1885~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FIL OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4FIL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4fil]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FIL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4FIL FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=0UE:FERRIOXAMINE+B'>0UE</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=0UE:FERRIOXAMINE+B'>0UE</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4fkm\|4fkm]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4fil FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fil OCA], [https://pdbe.org/4fil PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4fil RCSB], [https://www.ebi.ac.uk/pdbsum/4fil PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4fil ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">fhud2, SAV2284 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 "Micrococcus aureus" (Rosenbach 1884) Zopf 1885])</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4fil FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fil OCA], [~~http~~://pdbe.org/4fil PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4fil RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4fil PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4fil ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A0H3JWU6_STAAM A0A0H3JWU6_STAAM]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 25:		Line 25:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Briere, L K~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Heinrichs, D E~~]]	+	[[Category: Staphylococcus aureus]]
-	[[Category: ~~Shilton, B H~~]]	+	[[Category: Briere LK]]
-	[[Category: ~~Class iii solute binding protein~~]]	+	[[Category: Heinrichs DE]]
-	[[Category: ~~Extracellular]]~~	+	[[Category: Shilton BH]]
-	~~[[Category: Fhucbg]]~~	+
-	~~[[Category: Metal binding protein]]~~	+
-	~~[[Category: Primary binding site for iron-hydroxamate siderophore~~]]	+

OCA at 15:58, 11 August 2016

OCA — Thu, 11 Aug 2016 15:58:32 GMT

←Older revision		Revision as of 15:58, 11 August 2016
Line 1:		Line 1:
		+
	==Structure of FhuD2 from Staphylococcus Aureus with Bound Ferrioxamine B==		==Structure of FhuD2 from Staphylococcus Aureus with Bound Ferrioxamine B==
	<StructureSection load='4fil' size='340' side='right' caption='[[4fil]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='4fil' size='340' side='right' caption='[[4fil]], [[Resolution\|resolution]] 2.40Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4fkm\|4fkm]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4fkm\|4fkm]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">fhud2, SAV2284 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 "Micrococcus aureus" (Rosenbach 1884) Zopf 1885])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">fhud2, SAV2284 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 "Micrococcus aureus" (Rosenbach 1884) Zopf 1885])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fil FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fil OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4fil RCSB], [http://www.ebi.ac.uk/pdbsum/4fil PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fil FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fil OCA], [http://pdbe.org/4fil PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4fil RCSB], [http://www.ebi.ac.uk/pdbsum/4fil PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4fil ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4fil" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Ferric hydroxamate uptake receptor\|Ferric hydroxamate uptake receptor]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 12:23, 4 January 2015

OCA — Sun, 04 Jan 2015 12:23:11 GMT

←Older revision		Revision as of 12:23, 4 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4fil]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FIL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4FIL FirstGlance]. <br>		<table><tr><td colspan='2'>[[4fil]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FIL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4FIL FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=0UE:FERRIOXAMINE+B'>0UE</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=0UE:FERRIOXAMINE+B'>0UE</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4fkm\|4fkm]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4fkm\|4fkm]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">fhud2, SAV2284 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 "Micrococcus aureus" (Rosenbach 1884) Zopf 1885])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">fhud2, SAV2284 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 "Micrococcus aureus" (Rosenbach 1884) Zopf 1885])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fil FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fil OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4fil RCSB], [http://www.ebi.ac.uk/pdbsum/4fil PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fil FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fil OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4fil RCSB], [http://www.ebi.ac.uk/pdbsum/4fil PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 14:		Line 14:
	Crystal and solution structure analysis of FhuD2 from Staphylococcus aureus in multiple unliganded conformations and bound to ferrioxamine-B.,Podkowa KJ, Briere LA, Heinrichs DE, Shilton BH Biochemistry. 2014 Apr 1;53(12):2017-31. doi: 10.1021/bi401349d. Epub 2014 Mar, 19. PMID:24606332<ref>PMID:24606332</ref>		Crystal and solution structure analysis of FhuD2 from Staphylococcus aureus in multiple unliganded conformations and bound to ferrioxamine-B.,Podkowa KJ, Briere LA, Heinrichs DE, Shilton BH Biochemistry. 2014 Apr 1;53(12):2017-31. doi: 10.1021/bi401349d. Epub 2014 Mar, 19. PMID:24606332<ref>PMID:24606332</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==
Line 20:		Line 20:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Briere, L K.]]	+	[[Category: Briere, L K]]
-	[[Category: Heinrichs, D E.]]	+	[[Category: Heinrichs, D E]]
-	[[Category: Shilton, B H.]]	+	[[Category: Shilton, B H]]
	[[Category: Class iii solute binding protein]]		[[Category: Class iii solute binding protein]]
	[[Category: Extracellular]]		[[Category: Extracellular]]

OCA at 04:30, 4 June 2014

OCA — Wed, 04 Jun 2014 04:30:04 GMT

←Older revision		Revision as of 04:30, 4 June 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_4fil\| PDB=4fil \| SCENE= }}~~	+	==Structure of FhuD2 from Staphylococcus Aureus with Bound Ferrioxamine B==
-	===Structure of FhuD2 from Staphylococcus Aureus with Bound Ferrioxamine B===	+	<StructureSection load='4fil' size='340' side='right' caption='[[4fil]], [[Resolution\|resolution]] 2.40Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4fil]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FIL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4FIL FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=0UE:FERRIOXAMINE+B'>0UE</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4fkm\|4fkm]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">fhud2, SAV2284 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 "Micrococcus aureus" (Rosenbach 1884) Zopf 1885])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fil FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fil OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4fil RCSB], [http://www.ebi.ac.uk/pdbsum/4fil PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Iron acquisition is a central process for virtually all organisms. In Staphylococcus aureus, FhuD2 is a lipoprotein that is a high-affinity receptor for iron-bound hydroxamate siderophores. In this study, FhuD2 was crystallized bound to ferrioxamine-B (FXB), and also in its ligand-free state; the latter structures are the first for hydroxamate-binding receptors within this protein family. The structure of the FhuD2-FXB conformation shows that residues W197 and R199 from the C-terminal domain donate hydrogen bonds to the hydroxamate oxygens, and a ring of aromatic residues cradles the aliphatic arms connecting the hydroxamate moieties of the siderophore. The available ligand-bound structures of FhuD from Escherichia coli and YfiY from Bacillus cereus show that, despite a high degree of structural conservation, three protein families have evolved with critical siderophore binding residues on either the C-terminal domain (S. aureus), the N-terminal domain (E. coli), or both (B. cereus). Unliganded FhuD2 was crystallized in five conformations related by rigid body movements of the N- and C-terminal domains. Small-angle X-ray scattering (SAXS) indicates that the solution conformation of unliganded FhuD2 is more compact than the conformations observed in crystals. The ligand-induced conformational changes for FhuD2 in solution are relatively modest and depend on the identity of the siderophore. The crystallographic and SAXS results are used to discuss roles for the liganded and unliganded forms of FhuD2 in the siderophore transport mechanism.

-	~~==About this Structure==~~	+	Crystal and solution structure analysis of FhuD2 from Staphylococcus aureus in multiple unliganded conformations and bound to ferrioxamine-B.,Podkowa KJ, Briere LA, Heinrichs DE, Shilton BH Biochemistry. 2014 Apr 1;53(12):2017-31. doi: 10.1021/bi401349d. Epub 2014 Mar, 19. PMID:24606332<ref>PMID:24606332</ref>
-	~~[[4fil]] is a 4 chain~~ structure ~~with sequence~~ from ~~[http~~:~~//en~~.~~wikipedia~~.~~org~~/~~wiki/Staphylococcus_aureus Staphylococcus aureus]~~. ~~Full crystallographic information is available from [http~~://~~oca~~.~~weizmann~~.ac.il/~~oca-bin/ocashort?id~~=~~4FIL OCA].~~	+
-	~~[[Category: Staphylococcus aureus]]~~	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Briere, L K.]]		[[Category: Briere, L K.]]
	[[Category: Heinrichs, D E.]]		[[Category: Heinrichs, D E.]]

OCA at 21:03, 19 June 2013

OCA — Wed, 19 Jun 2013 21:03:14 GMT

←Older revision		Revision as of 21:03, 19 June 2013
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{STRUCTURE_4fil\| PDB=4fil \| SCENE= }}
		+	===Structure of FhuD2 from Staphylococcus Aureus with Bound Ferrioxamine B===

-	~~The entry~~ 4fil is ~~ON HOLD until Paper Publication~~	+	==About this Structure==
-		+	[[4fil]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FIL OCA].
-	~~Authors~~: Briere, L.K., Heinrichs, D.E., Shilton, B.H.	+	[[Category: Staphylococcus aureus]]
-		+	[[Category: Briere, L K.]]
-	~~Description~~: ~~Structure of FhuD2 from Staphylococcus Aureus with Bound Ferrioxamine B~~	+	[[Category: Heinrichs, D E.]]
		+	[[Category: Shilton, B H.]]
		+	[[Category: Class iii solute binding protein]]
		+	[[Category: Extracellular]]
		+	[[Category: Fhucbg]]
		+	[[Category: Metal binding protein]]
		+	[[Category: Primary binding site for iron-hydroxamate siderophore]]

OCA at 05:18, 15 August 2012

OCA — Wed, 15 Aug 2012 05:18:57 GMT

←Older revision		Revision as of 05:18, 15 August 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4fil is ON HOLD	+	The entry 4fil is ON HOLD until Paper Publication

	Authors: Briere, L.K., Heinrichs, D.E., Shilton, B.H.		Authors: Briere, L.K., Heinrichs, D.E., Shilton, B.H.

	Description: Structure of FhuD2 from Staphylococcus Aureus with Bound Ferrioxamine B		Description: Structure of FhuD2 from Staphylococcus Aureus with Bound Ferrioxamine B

OCA: Protected "4fil" [edit=sysop:move=sysop]

OCA — Wed, 20 Jun 2012 07:26:32 GMT

Protected "4fil" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:26, 20 June 2012

OCA: New page: '''Unreleased structure''' The entry 4fil is ON HOLD Authors: Briere, L.K., Heinrichs, D.E., Shilton, B.H. Description: Structure of FhuD2 from Staphylococcus Aureus with Bound Ferriox...

OCA — Wed, 20 Jun 2012 07:26:31 GMT

New page: '''Unreleased structure''' The entry 4fil is ON HOLD Authors: Briere, L.K., Heinrichs, D.E., Shilton, B.H. Description: Structure of FhuD2 from Staphylococcus Aureus with Bound Ferriox...

New page

'''Unreleased structure'''

The entry 4fil is ON HOLD

Authors: Briere, L.K., Heinrichs, D.E., Shilton, B.H.

Description: Structure of FhuD2 from Staphylococcus Aureus with Bound Ferrioxamine B