4fpq - Revision history

OCA: Replacing page with 'WITHDRAWN: The PDB entry 4FPQ was withdrawn.'

OCA — Wed, 08 Mar 2023 06:45:36 GMT

Replacing page with 'WITHDRAWN: The PDB entry 4FPQ was withdrawn.'

←Older revision		Revision as of 06:45, 8 March 2023
Line 1:		Line 1:
-		+	WITHDRAWN: The PDB entry 4FPQ was withdrawn.
-	~~==Structure of a fungal protein==~~	+
-	~~<StructureSection load='4fpq' size='340' side='right'caption='[[4fpq]], [[Resolution\|resolution]] 2.30Å' scene=''>~~	+
-	~~== Structural highlights ==~~	+
-	~~<table><tr><td colspan='2'>[[4fpq]] is a 4 chain structure with sequence from [https~~:~~//en.wikipedia.org/wiki/Leptosphaeria_maculans_JN3 Leptosphaeria maculans JN3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=~~4FPQ ~~OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4FPQ FirstGlance]. <br>~~	+
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4fpq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fpq OCA], [https://pdbe.org/4fpq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4fpq RCSB], [https://www.ebi.ac.uk/pdbsum/4fpq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4fpq ProSAT]</span></td></tr>	+
-	~~</table>~~	+
-	~~== Function ==~~	+
-	~~[https://www.uniprot.org/uniprot/E5A6Z5_LEPMJ E5A6Z5_LEPMJ]~~	+
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	The avirulence gene AvrLm4-7 of Leptosphaeria maculans, the causal agent of stem canker in Brassica napus (oilseed rape), confers a dual specificity of recognition by two resistance genes (Rlm4 and Rlm7) and is strongly involved in fungal fitness. In order to elucidate the biological function of AvrLm4-7 and understand the specificity of recognition by Rlm4 and Rlm7, the AvrLm4-7 protein was ~~produced in Pichia pastoris and its crystal structure was determined~~. It revealed the presence of four disulfide bridges, but no close structural analogs could be identified. A short stretch of amino acids in the C terminus of the protein, (R/N)(Y/F)(R/S)E(F/W), was well-conserved among AvrLm4-7 homologs. Loss of recognition of AvrLm4-7 by Rlm4 is caused by the mutation of a single glycine to an arginine residue located in a loop of the protein. Loss of recognition by Rlm7 is governed by more complex mutational patterns, including gene loss or drastic modifications of the protein structure. Three point mutations altered residues in the well-conserved C-terminal motif or close to the glycine involved in Rlm4-mediated recognition, resulting in the loss of Rlm7-mediated recognition. Transient expression in Nicotiana benthamiana (tobacco) and particle bombardment experiments on leaves from oilseed rape suggested that AvrLm4-7 interacts with its cognate R proteins inside the plant cell, and can be translocated into plant cells in the absence of the pathogen. Translocation of AvrLm4-7 into oilseed rape leaves is likely to require the (R/N)(Y/F)(R/S)E(F/W) motif as well as an RAWG motif located in a nearby loop that together form a positively charged region.	+
-		+
-	Crystal structure of the effector AvrLm4-7 of Leptosphaeria maculans reveals insights into its translocation into plant cells and recognition by resistance proteins.,Blondeau K, Blaise F, Graille M, Kale SD, Linglin J, Ollivier B, Labarde A, Lazar N, Daverdin G, Balesdent MH, Choi DH, Tyler BM, Rouxel T, van Tilbeurgh H, Fudal I Plant J. 2015 Aug;83(4):610-24. doi: 10.1111/tpj.12913. Epub 2015 Jul 14. PMID:26082394<ref>PMID:26082394</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 4fpq" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references/>~~	+
-	~~__TOC__~~	+
-	~~</StructureSection>~~	+
-	~~[[Category: Large Structures]]~~	+
-	~~[[Category: Leptosphaeria maculans JN3]]~~	+
-	~~[[Category: Balesdent MH]]~~	+
-	~~[[Category: Blaise F]]~~	+
-	~~[[Category: Blondeau K]]~~	+
-	~~[[Category: Daverdin G]]~~	+
-	~~[[Category: Doizy A]]~~	+
-	~~[[Category: Fudal I]]~~	+
-	~~[[Category: Graille M]]~~	+
-	~~[[Category: Labarde A]]~~	+
-	~~[[Category: Linglin J]]~~	+
-	~~[[Category: Ollivier B]]~~	+
-	~~[[Category: Rouxel T]]~~	+
-	~~[[Category: Van Tilbeurgh H]]~~	+

OCA at 05:57, 2 March 2023

OCA — Thu, 02 Mar 2023 05:57:38 GMT

←Older revision		Revision as of 05:57, 2 March 2023
Line 1:		Line 1:
-	~~WITHDRAWN~~: ~~The PDB entry~~ 4FPQ was ~~withdrawn~~.	+
		+	==Structure of a fungal protein==
		+	<StructureSection load='4fpq' size='340' side='right'caption='[[4fpq]], [[Resolution\|resolution]] 2.30Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4fpq]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Leptosphaeria_maculans_JN3 Leptosphaeria maculans JN3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FPQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4FPQ FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4fpq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fpq OCA], [https://pdbe.org/4fpq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4fpq RCSB], [https://www.ebi.ac.uk/pdbsum/4fpq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4fpq ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/E5A6Z5_LEPMJ E5A6Z5_LEPMJ]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The avirulence gene AvrLm4-7 of Leptosphaeria maculans, the causal agent of stem canker in Brassica napus (oilseed rape), confers a dual specificity of recognition by two resistance genes (Rlm4 and Rlm7) and is strongly involved in fungal fitness. In order to elucidate the biological function of AvrLm4-7 and understand the specificity of recognition by Rlm4 and Rlm7, the AvrLm4-7 protein was produced in Pichia pastoris and its crystal structure was determined. It revealed the presence of four disulfide bridges, but no close structural analogs could be identified. A short stretch of amino acids in the C terminus of the protein, (R/N)(Y/F)(R/S)E(F/W), was well-conserved among AvrLm4-7 homologs. Loss of recognition of AvrLm4-7 by Rlm4 is caused by the mutation of a single glycine to an arginine residue located in a loop of the protein. Loss of recognition by Rlm7 is governed by more complex mutational patterns, including gene loss or drastic modifications of the protein structure. Three point mutations altered residues in the well-conserved C-terminal motif or close to the glycine involved in Rlm4-mediated recognition, resulting in the loss of Rlm7-mediated recognition. Transient expression in Nicotiana benthamiana (tobacco) and particle bombardment experiments on leaves from oilseed rape suggested that AvrLm4-7 interacts with its cognate R proteins inside the plant cell, and can be translocated into plant cells in the absence of the pathogen. Translocation of AvrLm4-7 into oilseed rape leaves is likely to require the (R/N)(Y/F)(R/S)E(F/W) motif as well as an RAWG motif located in a nearby loop that together form a positively charged region.
		+
		+	Crystal structure of the effector AvrLm4-7 of Leptosphaeria maculans reveals insights into its translocation into plant cells and recognition by resistance proteins.,Blondeau K, Blaise F, Graille M, Kale SD, Linglin J, Ollivier B, Labarde A, Lazar N, Daverdin G, Balesdent MH, Choi DH, Tyler BM, Rouxel T, van Tilbeurgh H, Fudal I Plant J. 2015 Aug;83(4):610-24. doi: 10.1111/tpj.12913. Epub 2015 Jul 14. PMID:26082394<ref>PMID:26082394</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 4fpq" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Leptosphaeria maculans JN3]]
		+	[[Category: Balesdent MH]]
		+	[[Category: Blaise F]]
		+	[[Category: Blondeau K]]
		+	[[Category: Daverdin G]]
		+	[[Category: Doizy A]]
		+	[[Category: Fudal I]]
		+	[[Category: Graille M]]
		+	[[Category: Labarde A]]
		+	[[Category: Linglin J]]
		+	[[Category: Ollivier B]]
		+	[[Category: Rouxel T]]
		+	[[Category: Van Tilbeurgh H]]

OCA: Replacing page with 'WITHDRAWN: The PDB entry 4FPQ was withdrawn.'

OCA — Wed, 24 Jun 2020 23:25:05 GMT

Replacing page with 'WITHDRAWN: The PDB entry 4FPQ was withdrawn.'

←Older revision		Revision as of 23:25, 24 June 2020
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	WITHDRAWN: The PDB entry 4FPQ was withdrawn.
-		+
-	The entry ~~4fpq is ON HOLD~~	+
-		+
-	~~Authors: Blondeau, K~~.~~, Blaise, F., Graille, M., Linglin, J., Ollivier, B., Labarde, A., Doizy, A., Daverdin, G., Balesdent, MH., Rouxel, T., van Tilbeurgh, H., Fudal, I.~~	+
-		+
-	~~Description: Structure of a fungal protein~~	+
-	~~[[Category: Unreleased Structures]]~~	+
-	~~[[Category: Blaise, F]]~~	+
-	~~[[Category: Graille, M]]~~	+
-	~~[[Category: Labarde, A]]~~	+
-	~~[[Category: Linglin, J]]~~	+
-	~~[[Category: Balesdent, Mh]]~~	+
-	~~[[Category: Blondeau, K]]~~	+
-	~~[[Category: Van Tilbeurgh, H]]~~	+
-	~~[[Category: Rouxel, T]]~~	+
-	~~[[Category: Daverdin, G]]~~	+
-	~~[[Category: Fudal, I]]~~	+
-	~~[[Category: Ollivier, B]]~~	+
-	~~[[Category: Doizy, A]]~~	+

OCA at 10:37, 24 June 2020

OCA — Wed, 24 Jun 2020 10:37:39 GMT

←Older revision		Revision as of 10:37, 24 June 2020
Line 1:		Line 1:
-	~~WITHDRAWN:~~ The ~~PDB~~ entry ~~4FPQ was withdrawn~~.	+	'''Unreleased structure'''
		+
		+	The entry 4fpq is ON HOLD
		+
		+	Authors: Blondeau, K., Blaise, F., Graille, M., Linglin, J., Ollivier, B., Labarde, A., Doizy, A., Daverdin, G., Balesdent, MH., Rouxel, T., van Tilbeurgh, H., Fudal, I.
		+
		+	Description: Structure of a fungal protein
		+	[[Category: Unreleased Structures]]
		+	[[Category: Blaise, F]]
		+	[[Category: Graille, M]]
		+	[[Category: Labarde, A]]
		+	[[Category: Linglin, J]]
		+	[[Category: Balesdent, Mh]]
		+	[[Category: Blondeau, K]]
		+	[[Category: Van Tilbeurgh, H]]
		+	[[Category: Rouxel, T]]
		+	[[Category: Daverdin, G]]
		+	[[Category: Fudal, I]]
		+	[[Category: Ollivier, B]]
		+	[[Category: Doizy, A]]

OCA at 13:02, 19 June 2013

OCA — Wed, 19 Jun 2013 13:02:42 GMT

←Older revision		Revision as of 13:02, 19 June 2013
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	WITHDRAWN: The PDB entry 4FPQ was withdrawn.
-		+
-	The entry ~~4fpq is ON HOLD until Paper Publication~~	+
-		+
-	~~Authors: Blondeau, K~~.~~, Blaise, F., Graille, M., Linglin, J., Ollivier, B., Labarde, A., Doizy, A., Daverdin, G., Balesdent, MH., Rouxel, T., van Tilbeurgh, H., Fudal, I.~~	+
-		+
-	~~Description: Structure of a fungal protein~~	+

OCA: Protected "4fpq" [edit=sysop:move=sysop]

OCA — Wed, 04 Jul 2012 07:18:50 GMT

Protected "4fpq" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:18, 4 July 2012

OCA: New page: '''Unreleased structure''' The entry 4fpq is ON HOLD until Paper Publication Authors: Blondeau, K., Blaise, F., Graille, M., Linglin, J., Ollivier, B., Labarde, A., Doizy, A., Daverdin,...

OCA — Wed, 04 Jul 2012 07:18:49 GMT

New page: '''Unreleased structure''' The entry 4fpq is ON HOLD until Paper Publication Authors: Blondeau, K., Blaise, F., Graille, M., Linglin, J., Ollivier, B., Labarde, A., Doizy, A., Daverdin,...

New page

'''Unreleased structure'''

The entry 4fpq is ON HOLD until Paper Publication

Authors: Blondeau, K., Blaise, F., Graille, M., Linglin, J., Ollivier, B., Labarde, A., Doizy, A., Daverdin, G., Balesdent, MH., Rouxel, T., van Tilbeurgh, H., Fudal, I.

Description: Structure of a fungal protein