4fsk - Revision history

OCA at 14:19, 24 January 2024

OCA — Wed, 24 Jan 2024 14:19:02 GMT

←Older revision		Revision as of 14:19, 24 January 2024
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	<StructureSection load='4fsk' size='340' side='right'caption='[[4fsk]], [[Resolution\|resolution]] 1.98Å' scene=''>		<StructureSection load='4fsk' size='340' side='right'caption='[[4fsk]], [[Resolution\|resolution]] 1.98Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4fsk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Aspfl Aspfl~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FSK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4FSK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4fsk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_flavus Aspergillus flavus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FSK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4FSK FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=AZI:AZIDE+ION'>AZI</scene>~~, ~~<scene name='pdbligand=NA:SODIUM+ION'>NA</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.98Å</td></tr>
-	<tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=AZI:AZIDE+ION'>AZI</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-	~~<tr id~~='~~related'><td class~~=~~"sblockLbl"><b>[[Related_structure\|Related~~:~~]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;~~'>~~[[3p9f\|3p9f]], [[3p9o\|3p9o]]~~</~~div~~>~~</td></tr>~~	+
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">uaZ~~, ~~uox ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5059 ASPFL])~~<~~/td></tr>~~	+
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>~~[https://en.wikipedia.org/wiki/Factor_independent_urate_hydroxylase Factor independent urate hydroxylase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.3.3 1.7.3.3]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4fsk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fsk OCA], [https://pdbe.org/4fsk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4fsk RCSB], [https://www.ebi.ac.uk/pdbsum/4fsk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4fsk ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4fsk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fsk OCA], [https://pdbe.org/4fsk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4fsk RCSB], [https://www.ebi.ac.uk/pdbsum/4fsk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4fsk ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/URIC_ASPFL URIC_ASPFL]] Catalyzes the oxidation of uric acid to 5-hydroxyisourate, which is further processed to form (S)-allantoin.	+	[https://www.uniprot.org/uniprot/URIC_ASPFL URIC_ASPFL] Catalyzes the oxidation of uric acid to 5-hydroxyisourate, which is further processed to form (S)-allantoin.
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	BACKGROUND: Urate oxidase (EC 1.7.3.3 or UOX) catalyzes the conversion of uric acid and gaseous molecular oxygen to 5-hydroxyisourate and hydrogen peroxide, in the absence of cofactor or particular metal cation. The functional enzyme is a homo-tetramer with four active sites located at dimeric interfaces. RESULTS: The catalytic mechanism was investigated through a ternary complex formed between the enzyme, uric acid, and cyanide that stabilizes an intermediate state of the reaction. When uric acid is replaced by a competitive inhibitor, no complex with cyanide is formed. CONCLUSION: The X-ray structure of this compulsory ternary complex led to a number of mechanistic evidences that support a sequential mechanism in which the two reagents, dioxygen and a water molecule, process through a common site located 3.3 A above the mean plane of the ligand. This site is built by the side chains of Asn 254, and Thr 57, two conserved residues belonging to two different subunits of the homo-tetramer. The absence of a ternary complex between the enzyme, a competitive inhibitor, and cyanide suggests that cyanide inhibits the hydroxylation step of the reaction, after the initial formation of a hydroperoxyde type intermediate.	+
-		+
-	Structural analysis of urate oxidase in complex with its natural substrate inhibited by cyanide: mechanistic implications.,Gabison L, Prange T, Colloc'h N, El Hajji M, Castro B, Chiadmi M BMC Struct Biol. 2008 Jul 20;8:32. PMID:18638417<ref>PMID:18638417</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 4fsk" style="background-color:#fffaf0;"></div>~~	+

	==See Also==		==See Also==
-	*[[Urate ~~Oxidase~~\|Urate ~~Oxidase~~]]	+	*[[Urate oxidase 3D structures\|Urate oxidase 3D structures]]
-	~~== References ==~~	+
-	~~<references/>~~	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Aspfl]]~~	+	[[Category: Aspergillus flavus]]
-	~~[[Category: Factor independent urate hydroxylase~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Castro, B]]	+	[[Category: Castro B]]
-	[[Category: Chiadmi, M]]	+	[[Category: Chiadmi M]]
-	[[Category: ~~Gabison, L~~]]	+	[[Category: Colloc'h N]]
-	[[Category: Hajji, M El]]	+	[[Category: El Hajji M]]
-	[[Category: ~~Prange, T~~]]	+	[[Category: Gabison L]]
-	[[Category: ~~H, N Colloc]]~~	+	[[Category: Prange T]]
-	~~[[Category: Degradation mechanism]]~~	+
-	~~[[Category: Homotetramer]]~~	+
-	~~[[Category: Inhibition]]~~	+
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: Oxygen binding]]~~	+
-	~~[[Category: Peroxisome]]~~	+
-	~~[[Category: Purine metabolism~~]]	+

OCA at 02:58, 21 April 2022

OCA — Thu, 21 Apr 2022 02:58:57 GMT

←Older revision		Revision as of 02:58, 21 April 2022
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	==Urate oxidase-azide complex in anaerobic conditions==		==Urate oxidase-azide complex in anaerobic conditions==
-	<StructureSection load='4fsk' size='340' side='right' caption='[[4fsk]], [[Resolution\|resolution]] 1.98Å' scene=''>	+	<StructureSection load='4fsk' size='340' side='right'caption='[[4fsk]], [[Resolution\|resolution]] 1.98Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4fsk]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Aspfl Aspfl]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FSK OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4FSK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4fsk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspfl Aspfl]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FSK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4FSK FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=~~ACE~~:~~ACETYL~~+~~GROUP~~'>~~ACE~~</scene>, <scene name='pdbligand=~~AZI~~:~~AZIDE~~+ION'>~~AZI~~</scene>, <scene name='pdbligand=NA:~~SODIUM~~+~~ION~~'>NA</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AZI:AZIDE+ION'>AZI</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3p9f\|3p9f]], [[3p9o\|3p9o]]</td></tr>	+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">uaZ, uox ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5059 ASPFL])</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3p9f\|3p9f]], [[3p9o\|3p9o]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Factor_independent_urate_hydroxylase Factor independent urate hydroxylase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.3.3 1.7.3.3] </span></td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">uaZ, uox ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5059 ASPFL])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4fsk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fsk OCA], [~~http~~://pdbe.org/4fsk PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4fsk RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4fsk PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4fsk ProSAT]</span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Factor_independent_urate_hydroxylase Factor independent urate hydroxylase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.3.3 1.7.3.3] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4fsk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fsk OCA], [https://pdbe.org/4fsk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4fsk RCSB], [https://www.ebi.ac.uk/pdbsum/4fsk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4fsk ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/URIC_ASPFL URIC_ASPFL]] Catalyzes the oxidation of uric acid to 5-hydroxyisourate, which is further processed to form (S)-allantoin.	+	[[https://www.uniprot.org/uniprot/URIC_ASPFL URIC_ASPFL]] Catalyzes the oxidation of uric acid to 5-hydroxyisourate, which is further processed to form (S)-allantoin.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 4fsk" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4fsk" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Urate Oxidase\|Urate Oxidase]]
	== References ==		== References ==
	<references/>		<references/>
Line 27:		Line 31:
	[[Category: Aspfl]]		[[Category: Aspfl]]
	[[Category: Factor independent urate hydroxylase]]		[[Category: Factor independent urate hydroxylase]]
		+	[[Category: Large Structures]]
	[[Category: Castro, B]]		[[Category: Castro, B]]
	[[Category: Chiadmi, M]]		[[Category: Chiadmi, M]]

OCA at 07:03, 28 February 2018

OCA — Wed, 28 Feb 2018 07:03:24 GMT

←Older revision		Revision as of 07:03, 28 February 2018
Line 21:		Line 21:
	</div>		</div>
	<div class="pdbe-citations 4fsk" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4fsk" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Urate Oxidase\|Urate Oxidase]]
	== References ==		== References ==
	<references/>		<references/>
Line 33:		Line 30:
	[[Category: Chiadmi, M]]		[[Category: Chiadmi, M]]
	[[Category: Gabison, L]]		[[Category: Gabison, L]]
-	[[Category: H, N Colloc]]
	[[Category: Hajji, M El]]		[[Category: Hajji, M El]]
	[[Category: Prange, T]]		[[Category: Prange, T]]
		+	[[Category: H, N Colloc]]
	[[Category: Degradation mechanism]]		[[Category: Degradation mechanism]]
	[[Category: Homotetramer]]		[[Category: Homotetramer]]

OCA at 08:49, 4 August 2016

OCA — Thu, 04 Aug 2016 08:49:47 GMT

←Older revision		Revision as of 08:49, 4 August 2016
Line 1:		Line 1:
		+
	==Urate oxidase-azide complex in anaerobic conditions==		==Urate oxidase-azide complex in anaerobic conditions==
	<StructureSection load='4fsk' size='340' side='right' caption='[[4fsk]], [[Resolution\|resolution]] 1.98Å' scene=''>		<StructureSection load='4fsk' size='340' side='right' caption='[[4fsk]], [[Resolution\|resolution]] 1.98Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4fsk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Aspergillus_flavus Aspergillus flavus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FSK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4FSK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4fsk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aspfl Aspfl]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FSK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4FSK FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=AZI:AZIDE+ION'>AZI</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=AZI:AZIDE+ION'>AZI</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3p9f\|3p9f]], [[3p9o\|3p9o]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3p9f\|3p9f]], [[3p9o\|3p9o]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">uaZ, uox ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5059 ~~Aspergillus flavus~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">uaZ, uox ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5059 ASPFL])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Factor_independent_urate_hydroxylase Factor independent urate hydroxylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.3.3 1.7.3.3] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Factor_independent_urate_hydroxylase Factor independent urate hydroxylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.3.3 1.7.3.3] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fsk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fsk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4fsk RCSB], [http://www.ebi.ac.uk/pdbsum/4fsk PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fsk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fsk OCA], [http://pdbe.org/4fsk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4fsk RCSB], [http://www.ebi.ac.uk/pdbsum/4fsk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4fsk ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 19:		Line 20:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4fsk" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 26:		Line 28:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Aspergillus flavus~~]]	+	[[Category: Aspfl]]
	[[Category: Factor independent urate hydroxylase]]		[[Category: Factor independent urate hydroxylase]]
	[[Category: Castro, B]]		[[Category: Castro, B]]

OCA at 07:53, 24 December 2014

OCA — Wed, 24 Dec 2014 07:53:25 GMT

←Older revision		Revision as of 07:53, 24 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_4fsk\| PDB=4fsk \| SCENE= }}~~	+	==Urate oxidase-azide complex in anaerobic conditions==
-	===Urate oxidase-azide complex in anaerobic conditions===	+	<StructureSection load='4fsk' size='340' side='right' caption='[[4fsk]], [[Resolution\|resolution]] 1.98Å' scene=''>
-	~~{{ABSTRACT_PUBMED_18638417}}~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[4fsk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aspergillus_flavus Aspergillus flavus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FSK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4FSK FirstGlance]. <br>
-	==Function==	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=AZI:AZIDE+ION'>AZI</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3p9f\|3p9f]], [[3p9o\|3p9o]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">uaZ, uox ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5059 Aspergillus flavus])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Factor_independent_urate_hydroxylase Factor independent urate hydroxylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.3.3 1.7.3.3] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fsk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fsk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4fsk RCSB], [http://www.ebi.ac.uk/pdbsum/4fsk PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
	[[http://www.uniprot.org/uniprot/URIC_ASPFL URIC_ASPFL]] Catalyzes the oxidation of uric acid to 5-hydroxyisourate, which is further processed to form (S)-allantoin.		[[http://www.uniprot.org/uniprot/URIC_ASPFL URIC_ASPFL]] Catalyzes the oxidation of uric acid to 5-hydroxyisourate, which is further processed to form (S)-allantoin.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	BACKGROUND: Urate oxidase (EC 1.7.3.3 or UOX) catalyzes the conversion of uric acid and gaseous molecular oxygen to 5-hydroxyisourate and hydrogen peroxide, in the absence of cofactor or particular metal cation. The functional enzyme is a homo-tetramer with four active sites located at dimeric interfaces. RESULTS: The catalytic mechanism was investigated through a ternary complex formed between the enzyme, uric acid, and cyanide that stabilizes an intermediate state of the reaction. When uric acid is replaced by a competitive inhibitor, no complex with cyanide is formed. CONCLUSION: The X-ray structure of this compulsory ternary complex led to a number of mechanistic evidences that support a sequential mechanism in which the two reagents, dioxygen and a water molecule, process through a common site located 3.3 A above the mean plane of the ligand. This site is built by the side chains of Asn 254, and Thr 57, two conserved residues belonging to two different subunits of the homo-tetramer. The absence of a ternary complex between the enzyme, a competitive inhibitor, and cyanide suggests that cyanide inhibits the hydroxylation step of the reaction, after the initial formation of a hydroperoxyde type intermediate.

-	~~==About this Structure==~~	+	Structural analysis of urate oxidase in complex with its natural substrate inhibited by cyanide: mechanistic implications.,Gabison L, Prange T, Colloc'h N, El Hajji M, Castro B, Chiadmi M BMC Struct Biol. 2008 Jul 20;8:32. PMID:18638417<ref>PMID:18638417</ref>
-	~~[[4fsk]] is a 1 chain structure~~ with ~~sequence from [http~~:~~//en~~.~~wikipedia~~.~~org/wiki/Aspergillus_flavus Aspergillus flavus]~~. ~~Full crystallographic information is available from [http~~://~~oca~~.~~weizmann~~.ac.il/~~oca-bin/ocashort?id=4FSK OCA].~~	+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

	==See Also==		==See Also==
	*[[Urate Oxidase\|Urate Oxidase]]		*[[Urate Oxidase\|Urate Oxidase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	~~<ref group="xtra">PMID:018638417</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Aspergillus flavus]]		[[Category: Aspergillus flavus]]
	[[Category: Factor independent urate hydroxylase]]		[[Category: Factor independent urate hydroxylase]]
-	[[Category: Castro, B.]]	+	[[Category: Castro, B]]
-	[[Category: Chiadmi, M.]]	+	[[Category: Chiadmi, M]]
-	[[Category: Gabison, L.]]	+	[[Category: Gabison, L]]
-	[[Category: H, N Colloc.]]	+	[[Category: H, N Colloc]]
-	[[Category: Hajji, M El.]]	+	[[Category: Hajji, M El]]
-	[[Category: Prange, T.]]	+	[[Category: Prange, T]]
	[[Category: Degradation mechanism]]		[[Category: Degradation mechanism]]
	[[Category: Homotetramer]]		[[Category: Homotetramer]]

OCA at 04:04, 9 August 2013

OCA — Fri, 09 Aug 2013 04:04:10 GMT

←Older revision		Revision as of 04:04, 9 August 2013
Line 8:		Line 8:
	==About this Structure==		==About this Structure==
	[[4fsk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aspergillus_flavus Aspergillus flavus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FSK OCA].		[[4fsk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aspergillus_flavus Aspergillus flavus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FSK OCA].
		+
		+	==See Also==
		+	*[[Urate Oxidase\|Urate Oxidase]]

	==Reference==		==Reference==

OCA at 14:15, 3 July 2013

OCA — Wed, 03 Jul 2013 14:15:28 GMT

←Older revision		Revision as of 14:15, 3 July 2013
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{STRUCTURE_4fsk\| PDB=4fsk \| SCENE= }}
		+	===Urate oxidase-azide complex in anaerobic conditions===
		+	{{ABSTRACT_PUBMED_18638417}}

-	~~The entry 4fsk~~ is ~~ON HOLD until Paper Publication~~	+	==Function==
		+	[[http://www.uniprot.org/uniprot/URIC_ASPFL URIC_ASPFL]] Catalyzes the oxidation of uric acid to 5-hydroxyisourate, which is further processed to form (S)-allantoin.

-	~~Authors~~: ~~Gabison, L~~.~~, Colloc'H, N~~.~~, El Hajji, M~~.~~, Castro, B~~.~~, Chiadmi, M~~.~~, Prange, T~~.	+	==About this Structure==
		+	[[4fsk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aspergillus_flavus Aspergillus flavus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FSK OCA].

-	~~Description~~: ~~Urate oxidase-azide complex in anaerobic conditions~~	+	==Reference==
		+	<ref group="xtra">PMID:018638417</ref><references group="xtra"/><references/>
		+	[[Category: Aspergillus flavus]]
		+	[[Category: Factor independent urate hydroxylase]]
		+	[[Category: Castro, B.]]
		+	[[Category: Chiadmi, M.]]
		+	[[Category: Gabison, L.]]
		+	[[Category: H, N Colloc.]]
		+	[[Category: Hajji, M El.]]
		+	[[Category: Prange, T.]]
		+	[[Category: Degradation mechanism]]
		+	[[Category: Homotetramer]]
		+	[[Category: Inhibition]]
		+	[[Category: Oxidoreductase]]
		+	[[Category: Oxygen binding]]
		+	[[Category: Peroxisome]]
		+	[[Category: Purine metabolism]]

OCA at 05:20, 15 August 2012

OCA — Wed, 15 Aug 2012 05:20:10 GMT

←Older revision		Revision as of 05:20, 15 August 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4fsk is ON HOLD	+	The entry 4fsk is ON HOLD until Paper Publication

	Authors: Gabison, L., Colloc'H, N., El Hajji, M., Castro, B., Chiadmi, M., Prange, T.		Authors: Gabison, L., Colloc'H, N., El Hajji, M., Castro, B., Chiadmi, M., Prange, T.

	Description: Urate oxidase-azide complex in anaerobic conditions		Description: Urate oxidase-azide complex in anaerobic conditions

OCA at 05:25, 1 August 2012

OCA — Wed, 01 Aug 2012 05:25:48 GMT

←Older revision		Revision as of 05:25, 1 August 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4fsk is ON HOLD ~~until sometime in the future~~	+	The entry 4fsk is ON HOLD

	Authors: Gabison, L., Colloc'H, N., El Hajji, M., Castro, B., Chiadmi, M., Prange, T.		Authors: Gabison, L., Colloc'H, N., El Hajji, M., Castro, B., Chiadmi, M., Prange, T.

	Description: Urate oxidase-azide complex in anaerobic conditions		Description: Urate oxidase-azide complex in anaerobic conditions

OCA at 05:03, 18 July 2012

OCA — Wed, 18 Jul 2012 05:03:04 GMT

←Older revision		Revision as of 05:03, 18 July 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4fsk is ON HOLD	+	The entry 4fsk is ON HOLD until sometime in the future

	Authors: Gabison, L., Colloc'H, N., El Hajji, M., Castro, B., Chiadmi, M., Prange, T.		Authors: Gabison, L., Colloc'H, N., El Hajji, M., Castro, B., Chiadmi, M., Prange, T.

-	Description: ~~URATE OXIDASE~~-~~AZIDE COMPLEX IN ANAEROBIC CONDITIONS~~	+	Description: Urate oxidase-azide complex in anaerobic conditions