4fyz - Revision history

OCA at 15:36, 14 March 2024

OCA — Thu, 14 Mar 2024 15:36:33 GMT

←Older revision		Revision as of 15:36, 14 March 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4fyz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Citrobacter_braakii Citrobacter braakii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FYZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4FYZ FirstGlance]. <br>		<table><tr><td colspan='2'>[[4fyz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Citrobacter_braakii Citrobacter braakii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FYZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4FYZ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CNL:1,3,3-TRIMETHYL-2-OXABICYCLO[2.2.2]OCTANE'>CNL</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=NO:NITRIC+OXIDE'>NO</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.32Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CNL:1,3,3-TRIMETHYL-2-OXABICYCLO[2.2.2]OCTANE'>CNL</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=NO:NITRIC+OXIDE'>NO</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4fyz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fyz OCA], [https://pdbe.org/4fyz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4fyz RCSB], [https://www.ebi.ac.uk/pdbsum/4fyz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4fyz ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4fyz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fyz OCA], [https://pdbe.org/4fyz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4fyz RCSB], [https://www.ebi.ac.uk/pdbsum/4fyz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4fyz ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/CINA_CITBR CINA_CITBR] Involved in the degradation of cineol (eucalyptol). Catalyzes the initial hydroxylation of cineole exclusively at the pro-R carbon to give the (S)-6beta-hydroxycineole. Cineole is the natural substrate of CinA.		[https://www.uniprot.org/uniprot/CINA_CITBR CINA_CITBR] Involved in the degradation of cineol (eucalyptol). Catalyzes the initial hydroxylation of cineole exclusively at the pro-R carbon to give the (S)-6beta-hydroxycineole. Cineole is the natural substrate of CinA.
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The crystal structure of the P450cin substrate-bound nitric oxide complex and the substrate-free form have been determined. The substrate-free structure adopts an open conformation relative to the substrate-bound structure. The region of the I helix that forms part of the O2 binding pocket shifts from a-helix in the substrate-free form to a p helix in the substrate-bound form. Unique to P450cin is an active site residue, Asn242, in the I helix that H-bonds with the substrate. In most other P450s this residue is Thr which plays an important role in O2 activation by participating in a H-bonding network required for O2 activation. The p/a I helix transition results in the carbonyl O atom of Gly238 moving in to form an H-bond with the water/hydroxide ligand in the substrate-free form. The corresponding residue, Gly248, in P450cam experiences a similar motion and in the oxy-P450cam complex Gly248 adopts a position midway between the substrate-free and -bound states. A comparison between these P450cam and the new P450cin structures provides insights into differences in how the two P450s activate O2. In P450cin, NO and presumably O2, is positioned closer to Gly238 relative to how ligands bind in P450cam. This indicates that Gly238 forms a tighter interaction with diatomic ligands and is the key protein component in the active site that encourages reduction and protonation of the iron-linked O2 by accepting an H-bond from the hydroperoxy intermediate.
-
-	Crystal Structures of Substrate-Free and Nitrosyl Cytochrome P450cin: Implications for O2 Activation.,Madrona Y, Tripathi S, Li H, Poulos TL Biochemistry. 2012 Jul 9. PMID:22775403<ref>PMID:22775403</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 4fyz" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Cytochrome P450 3D structures\|Cytochrome P450 3D structures]]		*[[Cytochrome P450 3D structures\|Cytochrome P450 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 20:15, 19 October 2022

OCA — Wed, 19 Oct 2022 20:15:57 GMT

←Older revision		Revision as of 20:15, 19 October 2022
Line 1:		Line 1:

	==Crystal Structure of Nitrosyl Cytochrome P450cin==		==Crystal Structure of Nitrosyl Cytochrome P450cin==
-	<StructureSection load='4fyz' size='340' side='right' caption='[[4fyz]], [[Resolution\|resolution]] 2.32Å' scene=''>	+	<StructureSection load='4fyz' size='340' side='right'caption='[[4fyz]], [[Resolution\|resolution]] 2.32Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4fyz]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Atcc_51113 Atcc 51113~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FYZ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4FYZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4fyz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Citrobacter_braakii Citrobacter braakii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FYZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4FYZ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CNL:1,3,3-TRIMETHYL-2-OXABICYCLO[2.2.2]OCTANE'>CNL</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=NO:NITRIC+OXIDE'>NO</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CNL:1,3,3-TRIMETHYL-2-OXABICYCLO[2.2.2]OCTANE'>CNL</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=NO:NITRIC+OXIDE'>NO</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4fmx\|4fmx]], [[4fb2\|4fb2]], [[4g3r\|4g3r]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4fyz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fyz OCA], [https://pdbe.org/4fyz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4fyz RCSB], [https://www.ebi.ac.uk/pdbsum/4fyz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4fyz ProSAT]</span></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CIN A, cinA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=57706 ATCC 51113])~~</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4fyz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fyz OCA], [~~http~~://pdbe.org/4fyz PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4fyz RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4fyz PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4fyz ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/CINA_CITBR CINA_CITBR]] Involved in the degradation of cineol (eucalyptol). Catalyzes the initial hydroxylation of cineole exclusively at the pro-R carbon to give the (S)-6beta-hydroxycineole. Cineole is the natural substrate of CinA.	+	[https://www.uniprot.org/uniprot/CINA_CITBR CINA_CITBR] Involved in the degradation of cineol (eucalyptol). Catalyzes the initial hydroxylation of cineole exclusively at the pro-R carbon to give the (S)-6beta-hydroxycineole. Cineole is the natural substrate of CinA.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 20:

	==See Also==		==See Also==
-	*[[Cytochrome P450\|Cytochrome P450]]	+	*[[Cytochrome P450 3D structures\|Cytochrome P450 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Atcc 51113~~]]	+	[[Category: Citrobacter braakii]]
-	[[Category: ~~Li, H~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Madrona, Y~~]]	+	[[Category: Li H]]
-	[[Category: ~~Poulos, T L~~]]	+	[[Category: Madrona Y]]
-	[[Category: ~~Tripathi, S M~~]]	+	[[Category: Poulos TL]]
-	[[Category: ~~Cindoxin]]~~	+	[[Category: Tripathi SM]]
-	~~[[Category: Heme]]~~	+
-	~~[[Category: Monooxygenase]]~~	+
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: P450~~]]	+

OCA at 20:52, 11 August 2016

OCA — Thu, 11 Aug 2016 20:52:05 GMT

←Older revision		Revision as of 20:52, 11 August 2016
Line 1:		Line 1:
		+
	==Crystal Structure of Nitrosyl Cytochrome P450cin==		==Crystal Structure of Nitrosyl Cytochrome P450cin==
	<StructureSection load='4fyz' size='340' side='right' caption='[[4fyz]], [[Resolution\|resolution]] 2.32Å' scene=''>		<StructureSection load='4fyz' size='340' side='right' caption='[[4fyz]], [[Resolution\|resolution]] 2.32Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4fyz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Citrobacter_braakii Citrobacter braakii~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FYZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4FYZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4fyz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_51113 Atcc 51113]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FYZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4FYZ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CNL:1,3,3-TRIMETHYL-2-OXABICYCLO[2.2.2]OCTANE'>CNL</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=NO:NITRIC+OXIDE'>NO</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CNL:1,3,3-TRIMETHYL-2-OXABICYCLO[2.2.2]OCTANE'>CNL</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=NO:NITRIC+OXIDE'>NO</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4fmx\|4fmx]], [[4fb2\|4fb2]], [[4g3r\|4g3r]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4fmx\|4fmx]], [[4fb2\|4fb2]], [[4g3r\|4g3r]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CIN A, cinA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=57706 ~~Citrobacter braakii~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CIN A, cinA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=57706 ATCC 51113])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fyz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fyz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4fyz RCSB], [http://www.ebi.ac.uk/pdbsum/4fyz PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fyz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fyz OCA], [http://pdbe.org/4fyz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4fyz RCSB], [http://www.ebi.ac.uk/pdbsum/4fyz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4fyz ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[http://www.uniprot.org/uniprot/~~Q8VQF6_CITBR Q8VQF6_CITBR~~]] Involved in the degradation of cineol (eucalyptol). Catalyzes the initial hydroxylation of cineole exclusively at the pro-R carbon to give the (S)-6beta-hydroxycineole. Cineole is the natural substrate of CinA.	+	[[http://www.uniprot.org/uniprot/CINA_CITBR CINA_CITBR]] Involved in the degradation of cineol (eucalyptol). Catalyzes the initial hydroxylation of cineole exclusively at the pro-R carbon to give the (S)-6beta-hydroxycineole. Cineole is the natural substrate of CinA.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4fyz" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 25:		Line 27:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Citrobacter braakii~~]]	+	[[Category: Atcc 51113]]
	[[Category: Li, H]]		[[Category: Li, H]]
	[[Category: Madrona, Y]]		[[Category: Madrona, Y]]

OCA at 13:59, 4 January 2015

OCA — Sun, 04 Jan 2015 13:59:02 GMT

←Older revision		Revision as of 13:59, 4 January 2015
Line 1:		Line 1:
-	[[~~Image:~~4fyz.~~png~~\|~~left~~\|~~200px~~]]	+	==Crystal Structure of Nitrosyl Cytochrome P450cin==
		+	<StructureSection load='4fyz' size='340' side='right' caption='[[4fyz]], [[Resolution\|resolution]] 2.32Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4fyz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Citrobacter_braakii Citrobacter braakii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FYZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4FYZ FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CNL:1,3,3-TRIMETHYL-2-OXABICYCLO[2.2.2]OCTANE'>CNL</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=NO:NITRIC+OXIDE'>NO</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4fmx\|4fmx]], [[4fb2\|4fb2]], [[4g3r\|4g3r]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CIN A, cinA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=57706 Citrobacter braakii])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fyz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fyz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4fyz RCSB], [http://www.ebi.ac.uk/pdbsum/4fyz PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/Q8VQF6_CITBR Q8VQF6_CITBR]] Involved in the degradation of cineol (eucalyptol). Catalyzes the initial hydroxylation of cineole exclusively at the pro-R carbon to give the (S)-6beta-hydroxycineole. Cineole is the natural substrate of CinA.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The crystal structure of the P450cin substrate-bound nitric oxide complex and the substrate-free form have been determined. The substrate-free structure adopts an open conformation relative to the substrate-bound structure. The region of the I helix that forms part of the O2 binding pocket shifts from a-helix in the substrate-free form to a p helix in the substrate-bound form. Unique to P450cin is an active site residue, Asn242, in the I helix that H-bonds with the substrate. In most other P450s this residue is Thr which plays an important role in O2 activation by participating in a H-bonding network required for O2 activation. The p/a I helix transition results in the carbonyl O atom of Gly238 moving in to form an H-bond with the water/hydroxide ligand in the substrate-free form. The corresponding residue, Gly248, in P450cam experiences a similar motion and in the oxy-P450cam complex Gly248 adopts a position midway between the substrate-free and -bound states. A comparison between these P450cam and the new P450cin structures provides insights into differences in how the two P450s activate O2. In P450cin, NO and presumably O2, is positioned closer to Gly238 relative to how ligands bind in P450cam. This indicates that Gly238 forms a tighter interaction with diatomic ligands and is the key protein component in the active site that encourages reduction and protonation of the iron-linked O2 by accepting an H-bond from the hydroperoxy intermediate.

-	~~{{STRUCTURE_4fyz\| PDB=4fyz \| SCENE= }}~~	+	Crystal Structures of Substrate-Free and Nitrosyl Cytochrome P450cin: Implications for O2 Activation.,Madrona Y, Tripathi S, Li H, Poulos TL Biochemistry. 2012 Jul 9. PMID:22775403<ref>PMID:22775403</ref>

-	~~===Crystal Structure~~ of ~~Nitrosyl Cytochrome P450cin===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

-	~~{{ABSTRACT_PUBMED_22775403}}~~	+	==See Also==
-		+	*[[Cytochrome P450\|Cytochrome P450]]
-	==~~About this Structure~~==	+	== References ==
-	[[~~4fyz~~]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Citrobacter_braakii Citrobacter braakii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FYZ OCA].	+	<references/>
-		+	__TOC__
-	==~~Reference~~==	+	</StructureSection>
-	<~~ref group="xtra">PMID:022775403<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Citrobacter braakii]]		[[Category: Citrobacter braakii]]
-	[[Category: Li, H.]]	+	[[Category: Li, H]]
-	[[Category: Madrona, Y.]]	+	[[Category: Madrona, Y]]
-	[[Category: Poulos, T L.]]	+	[[Category: Poulos, T L]]
-	[[Category: Tripathi, S M.]]	+	[[Category: Tripathi, S M]]
	[[Category: Cindoxin]]		[[Category: Cindoxin]]
	[[Category: Heme]]		[[Category: Heme]]

OCA at 05:05, 1 August 2012

OCA — Wed, 01 Aug 2012 05:05:31 GMT

←Older revision		Revision as of 05:05, 1 August 2012
Line 1:		Line 1:
-	[[Image:4fyz.~~jpg~~\|left\|200px]]	+	[[Image:4fyz.png\|left\|200px]]

	{{STRUCTURE_4fyz\| PDB=4fyz \| SCENE= }}		{{STRUCTURE_4fyz\| PDB=4fyz \| SCENE= }}

OCA at 07:11, 25 July 2012

OCA — Wed, 25 Jul 2012 07:11:51 GMT

←Older revision		Revision as of 07:11, 25 July 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:4fyz.jpg\|left\|200px]]

-	~~The entry~~ 4fyz ~~is ON HOLD~~	+	{{STRUCTURE_4fyz\| PDB=4fyz \| SCENE= }}

-	~~Authors: Madrona, Y., Tripathi, S.M., Li, Huiying., Poulos, T.L.~~	+	===Crystal Structure of Nitrosyl Cytochrome P450cin===

-	~~Description: Crystal~~ Structure ~~of Nitrosyl Cytochrome P450cin~~	+	{{ABSTRACT_PUBMED_22775403}}
		+
		+	==About this Structure==
		+	[[4fyz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Citrobacter_braakii Citrobacter braakii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FYZ OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:022775403</ref><references group="xtra"/>
		+	[[Category: Citrobacter braakii]]
		+	[[Category: Li, H.]]
		+	[[Category: Madrona, Y.]]
		+	[[Category: Poulos, T L.]]
		+	[[Category: Tripathi, S M.]]
		+	[[Category: Cindoxin]]
		+	[[Category: Heme]]
		+	[[Category: Monooxygenase]]
		+	[[Category: Oxidoreductase]]
		+	[[Category: P450]]

OCA: Protected "4fyz" [edit=sysop:move=sysop]

OCA — Wed, 11 Jul 2012 07:54:12 GMT

Protected "4fyz" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:54, 11 July 2012

OCA: New page: '''Unreleased structure''' The entry 4fyz is ON HOLD Authors: Madrona, Y., Tripathi, S.M., Li, Huiying., Poulos, T.L. Description: Crystal Structure of Nitrosyl Cytochrome P450cin

OCA — Wed, 11 Jul 2012 07:54:11 GMT

New page: '''Unreleased structure''' The entry 4fyz is ON HOLD Authors: Madrona, Y., Tripathi, S.M., Li, Huiying., Poulos, T.L. Description: Crystal Structure of Nitrosyl Cytochrome P450cin

New page

'''Unreleased structure'''

The entry 4fyz is ON HOLD

Authors: Madrona, Y., Tripathi, S.M., Li, Huiying., Poulos, T.L.

Description: Crystal Structure of Nitrosyl Cytochrome P450cin