4fz4 - Revision history

OCA at 08:50, 20 March 2024

OCA — Wed, 20 Mar 2024 08:50:46 GMT

←Older revision		Revision as of 08:50, 20 March 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4fz4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_suis_98HAH33 Streptococcus suis 98HAH33]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FZ4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4FZ4 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4fz4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_suis_98HAH33 Streptococcus suis 98HAH33]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FZ4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4FZ4 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.44Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4fz4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fz4 OCA], [https://pdbe.org/4fz4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4fz4 RCSB], [https://www.ebi.ac.uk/pdbsum/4fz4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4fz4 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4fz4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fz4 OCA], [https://pdbe.org/4fz4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4fz4 RCSB], [https://www.ebi.ac.uk/pdbsum/4fz4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4fz4 ProSAT]</span></td></tr>
	</table>		</table>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Streptococcus suis, one of the most important and prevalent pathogens in swine, presents a major challenge to global public health. HP0197 is an S. suis surface antigen that was previously identified by immunoproteomics and can bind to the host cell surface. Here, we investigated the interaction between HP0197 and the host cell surface glycosaminoglycans (GAGs) using indirect immunofluorescence and cell adhesion inhibition assays. In addition, we determined that a novel 18-kDa domain in the N-terminal region of HP0197 functions as the GAG-binding domain. We then solved the three-dimensional structures of the N-terminal 18-kDa and C-terminal G5 domains using x-ray crystallography. Based on this structural information, the GAG-binding sites in HP0197 were predicted and subsequently verified using site-directed mutagenesis and indirect immunofluorescence. The results indicate that the positively charged residues on the exposed surface of the 18-kDa domain, which are primarily lysines, likely play a critical role in the HP0197-heparin interaction that mediates bacterium-host cell adhesion. Understanding this molecular mechanism may provide a basis for the development of effective drugs and therapeutic strategies for treating streptococcal infections.
-
-	Molecular mechanism by which surface antigen HP0197 mediates host cell attachment in the pathogenic bacteria Streptococcus suis.,Yuan ZZ, Yan XJ, Zhang AD, Chen B, Shen YQ, Jin ML J Biol Chem. 2013 Jan 11;288(2):956-63. doi: 10.1074/jbc.M112.388686. Epub 2012, Nov 26. PMID:23184929<ref>PMID:23184929</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 4fz4" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 20:16, 19 October 2022

OCA — Wed, 19 Oct 2022 20:16:08 GMT

←Older revision		Revision as of 20:16, 19 October 2022
Line 1:		Line 1:

	==Crystal structure of HP0197-18kd==		==Crystal structure of HP0197-18kd==
-	<StructureSection load='4fz4' size='340' side='right' caption='[[4fz4]], [[Resolution\|resolution]] 2.44Å' scene=''>	+	<StructureSection load='4fz4' size='340' side='right'caption='[[4fz4]], [[Resolution\|resolution]] 2.44Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4fz4]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Strs2 Strs2~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FZ4 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4FZ4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4fz4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_suis_98HAH33 Streptococcus suis 98HAH33]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FZ4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4FZ4 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SSU98_0197 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=391296 STRS2])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4fz4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fz4 OCA], [https://pdbe.org/4fz4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4fz4 RCSB], [https://www.ebi.ac.uk/pdbsum/4fz4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4fz4 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4fz4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fz4 OCA], [~~http~~://pdbe.org/4fz4 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4fz4 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4fz4 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4fz4 ProSAT]</span></td></tr>	+
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 21:		Line 20:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Strs2~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Yan, X~~]]	+	[[Category: Streptococcus suis 98HAH33]]
-	[[Category: ~~Yuan, Z~~]]	+	[[Category: Yan X]]
-	[[Category: ~~Immune system]]~~	+	[[Category: Yuan Z]]
-	~~[[Category: Surface antigen~~]]	+

OCA at 21:14, 11 August 2016

OCA — Thu, 11 Aug 2016 21:14:26 GMT

←Older revision		Revision as of 21:14, 11 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of HP0197-18kd==		==Crystal structure of HP0197-18kd==
	<StructureSection load='4fz4' size='340' side='right' caption='[[4fz4]], [[Resolution\|resolution]] 2.44Å' scene=''>		<StructureSection load='4fz4' size='340' side='right' caption='[[4fz4]], [[Resolution\|resolution]] 2.44Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4fz4]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Streptococcus_suis_98hah33 Streptococcus suis 98hah33~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FZ4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4FZ4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4fz4]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Strs2 Strs2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FZ4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4FZ4 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SSU98_0197 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=391296 ~~Streptococcus suis 98HAH33~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SSU98_0197 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=391296 STRS2])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fz4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fz4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4fz4 RCSB], [http://www.ebi.ac.uk/pdbsum/4fz4 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fz4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fz4 OCA], [http://pdbe.org/4fz4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4fz4 RCSB], [http://www.ebi.ac.uk/pdbsum/4fz4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4fz4 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 15:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4fz4" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Streptococcus suis 98hah33~~]]	+	[[Category: Strs2]]
	[[Category: Yan, X]]		[[Category: Yan, X]]
	[[Category: Yuan, Z]]		[[Category: Yuan, Z]]
	[[Category: Immune system]]		[[Category: Immune system]]
	[[Category: Surface antigen]]		[[Category: Surface antigen]]

OCA at 14:21, 4 January 2015

OCA — Sun, 04 Jan 2015 14:21:47 GMT

←Older revision		Revision as of 14:21, 4 January 2015
Line 1:		Line 1:
-	~~{{STRUCTURE_4fz4\| PDB~~=4fz4 \| ~~SCENE~~= }}	+	==Crystal structure of HP0197-18kd==
-	===~~Crystal~~ structure ~~of HP0197~~-~~18kd~~===	+	<StructureSection load='4fz4' size='340' side='right' caption='[[4fz4]], [[Resolution\|resolution]] 2.44Å' scene=''>
-	~~{{ABSTRACT_PUBMED_23184929}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4fz4]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_suis_98hah33 Streptococcus suis 98hah33]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FZ4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4FZ4 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SSU98_0197 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=391296 Streptococcus suis 98HAH33])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fz4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fz4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4fz4 RCSB], [http://www.ebi.ac.uk/pdbsum/4fz4 PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Streptococcus suis, one of the most important and prevalent pathogens in swine, presents a major challenge to global public health. HP0197 is an S. suis surface antigen that was previously identified by immunoproteomics and can bind to the host cell surface. Here, we investigated the interaction between HP0197 and the host cell surface glycosaminoglycans (GAGs) using indirect immunofluorescence and cell adhesion inhibition assays. In addition, we determined that a novel 18-kDa domain in the N-terminal region of HP0197 functions as the GAG-binding domain. We then solved the three-dimensional structures of the N-terminal 18-kDa and C-terminal G5 domains using x-ray crystallography. Based on this structural information, the GAG-binding sites in HP0197 were predicted and subsequently verified using site-directed mutagenesis and indirect immunofluorescence. The results indicate that the positively charged residues on the exposed surface of the 18-kDa domain, which are primarily lysines, likely play a critical role in the HP0197-heparin interaction that mediates bacterium-host cell adhesion. Understanding this molecular mechanism may provide a basis for the development of effective drugs and therapeutic strategies for treating streptococcal infections.

-	~~==About this Structure==~~	+	Molecular mechanism by which surface antigen HP0197 mediates host cell attachment in the pathogenic bacteria Streptococcus suis.,Yuan ZZ, Yan XJ, Zhang AD, Chen B, Shen YQ, Jin ML J Biol Chem. 2013 Jan 11;288(2):956-63. doi: 10.1074/jbc.M112.388686. Epub 2012, Nov 26. PMID:23184929<ref>PMID:23184929</ref>
-	~~[[4fz4]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_suis_98hah33~~ Streptococcus suis ~~98hah33]~~. ~~Full crystallographic information is available from [http~~:/~~/oca~~.~~weizmann~~.ac.il/~~oca-bin/ocashort?id=4FZ4 OCA].~~	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group="xtra">PMID:023184929</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Streptococcus suis 98hah33]]		[[Category: Streptococcus suis 98hah33]]
-	[[Category: Yan, X.]]	+	[[Category: Yan, X]]
-	[[Category: Yuan, Z.]]	+	[[Category: Yuan, Z]]
	[[Category: Immune system]]		[[Category: Immune system]]
	[[Category: Surface antigen]]		[[Category: Surface antigen]]

OCA at 09:01, 4 September 2013

OCA — Wed, 04 Sep 2013 09:01:06 GMT

←Older revision		Revision as of 09:01, 4 September 2013
Line 1:		Line 1:
-	[[Image:4fz4.jpg\|left\|200px]]
-
	{{STRUCTURE_4fz4\| PDB=4fz4 \| SCENE= }}		{{STRUCTURE_4fz4\| PDB=4fz4 \| SCENE= }}
-
	===Crystal structure of HP0197-18kd===		===Crystal structure of HP0197-18kd===
-		+	{{ABSTRACT_PUBMED_23184929}}

	==About this Structure==		==About this Structure==
	[[4fz4]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_suis_98hah33 Streptococcus suis 98hah33]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FZ4 OCA].		[[4fz4]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_suis_98hah33 Streptococcus suis 98hah33]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FZ4 OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:023184929</ref><references group="xtra"/><references/>
	[[Category: Streptococcus suis 98hah33]]		[[Category: Streptococcus suis 98hah33]]
	[[Category: Yan, X.]]		[[Category: Yan, X.]]

OCA at 11:00, 5 December 2012

OCA — Wed, 05 Dec 2012 11:00:03 GMT

←Older revision		Revision as of 11:00, 5 December 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:4fz4.jpg\|left\|200px]]

-	~~The entry~~ 4fz4 ~~is ON HOLD~~ ~~until Paper Publication~~	+	{{STRUCTURE_4fz4\| PDB=4fz4 \| SCENE= }}

-	~~Authors: Yuan, Z., Yan, X.~~	+	===Crystal structure of HP0197-18kd===

-	~~Description: Crystal~~ structure ~~of HP0197~~-~~18kd~~	+
		+	==About this Structure==
		+	[[4fz4]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_suis_98hah33 Streptococcus suis 98hah33]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FZ4 OCA].
		+	[[Category: Streptococcus suis 98hah33]]
		+	[[Category: Yan, X.]]
		+	[[Category: Yuan, Z.]]
		+	[[Category: Immune system]]
		+	[[Category: Surface antigen]]

OCA at 04:56, 8 August 2012

OCA — Wed, 08 Aug 2012 04:56:11 GMT

←Older revision		Revision as of 04:56, 8 August 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4fz4 is ON HOLD	+	The entry 4fz4 is ON HOLD until Paper Publication

-	Authors: ~~Yan~~, Z.	+	Authors: Yuan, Z., Yan, X.

	Description: Crystal structure of HP0197-18kd		Description: Crystal structure of HP0197-18kd

OCA at 06:49, 25 July 2012

OCA — Wed, 25 Jul 2012 06:49:37 GMT

←Older revision		Revision as of 06:49, 25 July 2012
Line 3:		Line 3:
	The entry 4fz4 is ON HOLD		The entry 4fz4 is ON HOLD

-	Authors: ~~Xiaojie~~ Yan	+	Authors: Yan, Z.

	Description: Crystal structure of HP0197-18kd		Description: Crystal structure of HP0197-18kd

OCA: Protected "4fz4" [edit=sysop:move=sysop]

OCA — Wed, 11 Jul 2012 07:54:20 GMT

Protected "4fz4" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:54, 11 July 2012

OCA: New page: '''Unreleased structure''' The entry 4fz4 is ON HOLD Authors: Xiaojie Yan Description: Crystal structure of HP0197-18kd

OCA — Wed, 11 Jul 2012 07:54:19 GMT

New page: '''Unreleased structure''' The entry 4fz4 is ON HOLD Authors: Xiaojie Yan Description: Crystal structure of HP0197-18kd

New page

'''Unreleased structure'''

The entry 4fz4 is ON HOLD

Authors: Xiaojie Yan

Description: Crystal structure of HP0197-18kd