4g4i - Revision history

OCA at 15:39, 14 March 2024

OCA — Thu, 14 Mar 2024 15:39:09 GMT

←Older revision		Revision as of 15:39, 14 March 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4g4i]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermothelomyces_thermophilus_ATCC_42464 Thermothelomyces thermophilus ATCC 42464]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G4I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4G4I FirstGlance]. <br>		<table><tr><td colspan='2'>[[4g4i]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermothelomyces_thermophilus_ATCC_42464 Thermothelomyces thermophilus ATCC 42464]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G4I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4G4I FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.9Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4g4i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4g4i OCA], [https://pdbe.org/4g4i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4g4i RCSB], [https://www.ebi.ac.uk/pdbsum/4g4i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4g4i ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4g4i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4g4i OCA], [https://pdbe.org/4g4i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4g4i RCSB], [https://www.ebi.ac.uk/pdbsum/4g4i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4g4i ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/GCE2_MYCTT GCE2_MYCTT] Glucuronoyl esterase which may play a significant role in biomass degradation, as it is considered to disconnect hemicellulose from lignin through the hydrolysis of the ester bond between 4-O-methyl-D-glucuronic acid residues of glucuronoxylans and aromatic alcohols of lignin.<ref>PMID:20473662</ref>		[https://www.uniprot.org/uniprot/GCE2_MYCTT GCE2_MYCTT] Glucuronoyl esterase which may play a significant role in biomass degradation, as it is considered to disconnect hemicellulose from lignin through the hydrolysis of the ester bond between 4-O-methyl-D-glucuronic acid residues of glucuronoxylans and aromatic alcohols of lignin.<ref>PMID:20473662</ref>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The increasing demand for the development of efficient biocatalysts is a consequence of their broad industrial applications. Typical difficulties that are encountered during their exploitation in a variety of processes are interconnected with factors such as temperature, pH, product inhibitors etc. To eliminate these, research has been directed towards the identification of new enzymes that would comply with the required standards. To this end, the recently discovered glucuronoyl esterases (GEs) are an enigmatic family within the carbohydrate esterase (CE) family. Structures of the thermophilic StGE2 esterase from Myceliophthora thermophila (synonym Sporotrichum thermophile), a member of the CE15 family, and its S213A mutant were determined at 1.55 and 1.9 A resolution, respectively. The first crystal structure of the S213A mutant in complex with a substrate analogue, methyl 4-O-methyl-beta-D-glucopyranuronate, was determined at 2.35 A resolution. All of the three-dimensional protein structures have an alpha/beta-hydrolase fold with a three-layer alphabetaalpha-sandwich architecture and a Rossmann topology and comprise one molecule per asymmetric unit. These are the first crystal structures of a thermophilic GE both in an unliganded form and bound to a substrate analogue, thus unravelling the organization of the catalytic triad residues and their neighbours lining the active site. The knowledge derived offers novel insights into the key structural elements that drive the hydrolysis of glucuronic acid esters.
-
-	The structure of a novel glucuronoyl esterase from Myceliophthora thermophila gives new insights into its role as a potential biocatalyst.,Charavgi MD, Dimarogona M, Topakas E, Christakopoulos P, Chrysina ED Acta Crystallogr D Biol Crystallogr. 2013 Jan;69(Pt 1):63-73. doi:, 10.1107/S0907444912042400. Epub 2012 Dec 20. PMID:23275164<ref>PMID:23275164</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 4g4i" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 20:24, 19 October 2022

OCA — Wed, 19 Oct 2022 20:24:18 GMT

←Older revision		Revision as of 20:24, 19 October 2022
Line 1:		Line 1:

	==Crystal structure of glucuronoyl esterase S213A mutant from Sporotrichum thermophile determined at 1.9 A resolution==		==Crystal structure of glucuronoyl esterase S213A mutant from Sporotrichum thermophile determined at 1.9 A resolution==
-	<StructureSection load='4g4i' size='340' side='right' caption='[[4g4i]], [[Resolution\|resolution]] 1.90Å' scene=''>	+	<StructureSection load='4g4i' size='340' side='right'caption='[[4g4i]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4g4i]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Myctt Myctt~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G4I OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4G4I FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4g4i]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermothelomyces_thermophilus_ATCC_42464 Thermothelomyces thermophilus ATCC 42464]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G4I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4G4I FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4g4g\|4g4g]], [[4g4j\|4g4j]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4g4i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4g4i OCA], [https://pdbe.org/4g4i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4g4i RCSB], [https://www.ebi.ac.uk/pdbsum/4g4i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4g4i ProSAT]</span></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MYCTH_55568 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=573729 MYCTT])~~</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4g4i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4g4i OCA], [~~http~~://pdbe.org/4g4i PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4g4i RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4g4i PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4g4i ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/~~CIP2_THIHA CIP2_THIHA]~~] Glucuronoyl esterase which may play a significant role in biomass degradation, as it is considered to disconnect hemicellulose from lignin through the hydrolysis of the ester bond between 4-O-methyl-D-glucuronic acid residues of glucuronoxylans and aromatic alcohols of lignin.<ref>PMID:20473662</ref>	+	[https://www.uniprot.org/uniprot/GCE2_MYCTT GCE2_MYCTT] Glucuronoyl esterase which may play a significant role in biomass degradation, as it is considered to disconnect hemicellulose from lignin through the hydrolysis of the ester bond between 4-O-methyl-D-glucuronic acid residues of glucuronoxylans and aromatic alcohols of lignin.<ref>PMID:20473662</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 20:

	==See Also==		==See Also==
-	*[[Methylesterase\|Methylesterase]]	+	*[[Methylesterase 3D structures\|Methylesterase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Myctt~~]]	+	[[Category: Large Structures]]
-	[[Category: Charvagi~~, M D~~]]	+	[[Category: Thermothelomyces thermophilus ATCC 42464]]
-	[[Category: Christakopoulos, P]]	+	[[Category: Charvagi MD]]
-	[[Category: Chrysina~~, E D~~]]	+	[[Category: Christakopoulos P]]
-	[[Category: Dimarogona, M]]	+	[[Category: Chrysina ED]]
-	[[Category: Topakas, E]]	+	[[Category: Dimarogona M]]
-	~~[[Category: 3-layer alpha/beta/alpha sandwich]]~~	+	[[Category: Topakas E]]
-	~~[[Category: Alpha/beta hydrolase]]~~	+
-	~~[[Category: Glucuronoyl esterase]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Rossmann fold~~]]	+

OCA at 06:39, 4 April 2018

OCA — Wed, 04 Apr 2018 06:39:14 GMT

←Older revision		Revision as of 06:39, 4 April 2018
Line 3:		Line 3:
	<StructureSection load='4g4i' size='340' side='right' caption='[[4g4i]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='4g4i' size='340' side='right' caption='[[4g4i]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4g4i]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Myceliophthora_thermophila Myceliophthora thermophila~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G4I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4G4I FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4g4i]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Myctt Myctt]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G4I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4G4I FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4g4g\|4g4g]], [[4g4j\|4g4j]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4g4g\|4g4g]], [[4g4j\|4g4j]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MYCTH_55568 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=573729 ~~Myceliophthora thermophila~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MYCTH_55568 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=573729 MYCTT])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4g4i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4g4i OCA], [http://pdbe.org/4g4i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4g4i RCSB], [http://www.ebi.ac.uk/pdbsum/4g4i PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4g4i ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4g4i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4g4i OCA], [http://pdbe.org/4g4i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4g4i RCSB], [http://www.ebi.ac.uk/pdbsum/4g4i PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4g4i ProSAT]</span></td></tr>
	</table>		</table>
Line 27:		Line 27:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Myceliophthora thermophila~~]]	+	[[Category: Myctt]]
	[[Category: Charvagi, M D]]		[[Category: Charvagi, M D]]
	[[Category: Christakopoulos, P]]		[[Category: Christakopoulos, P]]

OCA at 15:49, 4 August 2016

OCA — Thu, 04 Aug 2016 15:49:41 GMT

←Older revision		Revision as of 15:49, 4 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of glucuronoyl esterase S213A mutant from Sporotrichum thermophile determined at 1.9 A resolution==		==Crystal structure of glucuronoyl esterase S213A mutant from Sporotrichum thermophile determined at 1.9 A resolution==
	<StructureSection load='4g4i' size='340' side='right' caption='[[4g4i]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='4g4i' size='340' side='right' caption='[[4g4i]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4g4i]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Myceliophthora_thermophila_atcc_42464~~ Myceliophthora thermophila ~~atcc 42464~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G4I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4G4I FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4g4i]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Myceliophthora_thermophila Myceliophthora thermophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G4I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4G4I FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4g4g\|4g4g]], [[4g4j\|4g4j]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4g4g\|4g4g]], [[4g4j\|4g4j]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MYCTH_55568 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=573729 Myceliophthora thermophila ~~ATCC 42464~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MYCTH_55568 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=573729 Myceliophthora thermophila])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4g4i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4g4i OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4g4i RCSB], [http://www.ebi.ac.uk/pdbsum/4g4i PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4g4i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4g4i OCA], [http://pdbe.org/4g4i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4g4i RCSB], [http://www.ebi.ac.uk/pdbsum/4g4i PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4g4i ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4g4i" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 25:		Line 27:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Myceliophthora thermophila ~~atcc 42464~~]]	+	[[Category: Myceliophthora thermophila]]
	[[Category: Charvagi, M D]]		[[Category: Charvagi, M D]]
	[[Category: Christakopoulos, P]]		[[Category: Christakopoulos, P]]

OCA at 16:40, 24 December 2014

OCA — Wed, 24 Dec 2014 16:40:15 GMT

←Older revision		Revision as of 16:40, 24 December 2014
Line 1:		Line 1:
-	[[~~Image:~~4g4i.~~png~~\|~~left~~\|~~200px~~]]	+	==Crystal structure of glucuronoyl esterase S213A mutant from Sporotrichum thermophile determined at 1.9 A resolution==
		+	<StructureSection load='4g4i' size='340' side='right' caption='[[4g4i]], [[Resolution\|resolution]] 1.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4g4i]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Myceliophthora_thermophila_atcc_42464 Myceliophthora thermophila atcc 42464]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G4I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4G4I FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4g4g\|4g4g]], [[4g4j\|4g4j]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MYCTH_55568 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=573729 Myceliophthora thermophila ATCC 42464])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4g4i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4g4i OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4g4i RCSB], [http://www.ebi.ac.uk/pdbsum/4g4i PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/CIP2_THIHA CIP2_THIHA]] Glucuronoyl esterase which may play a significant role in biomass degradation, as it is considered to disconnect hemicellulose from lignin through the hydrolysis of the ester bond between 4-O-methyl-D-glucuronic acid residues of glucuronoxylans and aromatic alcohols of lignin.<ref>PMID:20473662</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The increasing demand for the development of efficient biocatalysts is a consequence of their broad industrial applications. Typical difficulties that are encountered during their exploitation in a variety of processes are interconnected with factors such as temperature, pH, product inhibitors etc. To eliminate these, research has been directed towards the identification of new enzymes that would comply with the required standards. To this end, the recently discovered glucuronoyl esterases (GEs) are an enigmatic family within the carbohydrate esterase (CE) family. Structures of the thermophilic StGE2 esterase from Myceliophthora thermophila (synonym Sporotrichum thermophile), a member of the CE15 family, and its S213A mutant were determined at 1.55 and 1.9 A resolution, respectively. The first crystal structure of the S213A mutant in complex with a substrate analogue, methyl 4-O-methyl-beta-D-glucopyranuronate, was determined at 2.35 A resolution. All of the three-dimensional protein structures have an alpha/beta-hydrolase fold with a three-layer alphabetaalpha-sandwich architecture and a Rossmann topology and comprise one molecule per asymmetric unit. These are the first crystal structures of a thermophilic GE both in an unliganded form and bound to a substrate analogue, thus unravelling the organization of the catalytic triad residues and their neighbours lining the active site. The knowledge derived offers novel insights into the key structural elements that drive the hydrolysis of glucuronic acid esters.

-	~~{{STRUCTURE_4g4i\| PDB=4g4i \| SCENE= }}~~	+	The structure of a novel glucuronoyl esterase from Myceliophthora thermophila gives new insights into its role as a potential biocatalyst.,Charavgi MD, Dimarogona M, Topakas E, Christakopoulos P, Chrysina ED Acta Crystallogr D Biol Crystallogr. 2013 Jan;69(Pt 1):63-73. doi:, 10.1107/S0907444912042400. Epub 2012 Dec 20. PMID:23275164<ref>PMID:23275164</ref>

-	~~===Crystal structure~~ of ~~glucuronoyl esterase S213A mutant from Sporotrichum thermophile determined at 1~~.~~9 A resolution===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

-	~~{{ABSTRACT_PUBMED_23275164}}~~	+	==See Also==
-		+	*[[Methylesterase\|Methylesterase]]
-	==~~About this Structure~~==	+	== References ==
-	[[~~4g4i~~]] ~~is a 1 chain structure with sequence from [http:~~//en.wikipedia.org/wiki/Myceliophthora_thermophila_atcc_42464 Myceliophthora thermophila atcc 42464]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G4I OCA].	+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Myceliophthora thermophila atcc 42464]]		[[Category: Myceliophthora thermophila atcc 42464]]
-	[[Category: Charvagi, M D.]]	+	[[Category: Charvagi, M D]]
-	[[Category: Christakopoulos, P.]]	+	[[Category: Christakopoulos, P]]
-	[[Category: Chrysina, E D.]]	+	[[Category: Chrysina, E D]]
-	[[Category: Dimarogona, M.]]	+	[[Category: Dimarogona, M]]
-	[[Category: Topakas, E.]]	+	[[Category: Topakas, E]]
	[[Category: 3-layer alpha/beta/alpha sandwich]]		[[Category: 3-layer alpha/beta/alpha sandwich]]
	[[Category: Alpha/beta hydrolase]]		[[Category: Alpha/beta hydrolase]]

OCA at 11:38, 23 January 2013

OCA — Wed, 23 Jan 2013 11:38:12 GMT

←Older revision		Revision as of 11:38, 23 January 2013
Line 1:		Line 1:
-	[[Image:4g4i.~~jpg~~\|left\|200px]]	+	[[Image:4g4i.png\|left\|200px]]

	{{STRUCTURE_4g4i\| PDB=4g4i \| SCENE= }}		{{STRUCTURE_4g4i\| PDB=4g4i \| SCENE= }}
Line 5:		Line 5:
	===Crystal structure of glucuronoyl esterase S213A mutant from Sporotrichum thermophile determined at 1.9 A resolution===		===Crystal structure of glucuronoyl esterase S213A mutant from Sporotrichum thermophile determined at 1.9 A resolution===

		+	{{ABSTRACT_PUBMED_23275164}}

	==About this Structure==		==About this Structure==

OCA at 09:35, 2 January 2013

OCA — Wed, 02 Jan 2013 09:35:57 GMT

←Older revision		Revision as of 09:35, 2 January 2013
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:4g4i.jpg\|left\|200px]]

-	~~The entry~~ 4g4i ~~is ON HOLD~~ ~~until Jul 16 2014~~	+	{{STRUCTURE_4g4i\| PDB=4g4i \| SCENE= }}

-	~~Authors: Charvagi, M.D., Dimarogona, M., Topakas, E., Christakopoulos, P., Chrysina, E.D~~.	+	===Crystal structure of glucuronoyl esterase S213A mutant from Sporotrichum thermophile determined at 1.9 A resolution===

-	~~Description: Crystal~~ structure ~~of glucuronoyl esterase S213A mutant~~ from ~~Sporotrichum thermophile determined at 1~~.~~9 A resolution~~	+
		+	==About this Structure==
		+	[[4g4i]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Myceliophthora_thermophila_atcc_42464 Myceliophthora thermophila atcc 42464]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G4I OCA].
		+	[[Category: Myceliophthora thermophila atcc 42464]]
		+	[[Category: Charvagi, M D.]]
		+	[[Category: Christakopoulos, P.]]
		+	[[Category: Chrysina, E D.]]
		+	[[Category: Dimarogona, M.]]
		+	[[Category: Topakas, E.]]
		+	[[Category: 3-layer alpha/beta/alpha sandwich]]
		+	[[Category: Alpha/beta hydrolase]]
		+	[[Category: Glucuronoyl esterase]]
		+	[[Category: Hydrolase]]
		+	[[Category: Rossmann fold]]

OCA at 05:30, 22 August 2012

OCA — Wed, 22 Aug 2012 05:30:55 GMT

←Older revision		Revision as of 05:30, 22 August 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4g4i is ON HOLD	+	The entry 4g4i is ON HOLD until Jul 16 2014

	Authors: Charvagi, M.D., Dimarogona, M., Topakas, E., Christakopoulos, P., Chrysina, E.D.		Authors: Charvagi, M.D., Dimarogona, M., Topakas, E., Christakopoulos, P., Chrysina, E.D.

	Description: Crystal structure of glucuronoyl esterase S213A mutant from Sporotrichum thermophile determined at 1.9 A resolution		Description: Crystal structure of glucuronoyl esterase S213A mutant from Sporotrichum thermophile determined at 1.9 A resolution

OCA: Protected "4g4i" [edit=sysop:move=sysop]

OCA — Wed, 25 Jul 2012 06:52:06 GMT

Protected "4g4i" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:52, 25 July 2012

OCA: New page: '''Unreleased structure''' The entry 4g4i is ON HOLD Authors: Charvagi, M.D., Dimarogona, M., Topakas, E., Christakopoulos, P., Chrysina, E.D. Description: Crystal structure of glucuro...

OCA — Wed, 25 Jul 2012 06:52:06 GMT

New page: '''Unreleased structure''' The entry 4g4i is ON HOLD Authors: Charvagi, M.D., Dimarogona, M., Topakas, E., Christakopoulos, P., Chrysina, E.D. Description: Crystal structure of glucuro...

New page

'''Unreleased structure'''

The entry 4g4i is ON HOLD

Authors: Charvagi, M.D., Dimarogona, M., Topakas, E., Christakopoulos, P., Chrysina, E.D.

Description: Crystal structure of glucuronoyl esterase S213A mutant from Sporotrichum thermophile determined at 1.9 A resolution