4g63 - Revision history

OCA at 10:35, 11 August 2016

OCA — Thu, 11 Aug 2016 10:35:59 GMT

←Older revision		Revision as of 10:35, 11 August 2016
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		+
	==Crystal structure of cytosolic IMP-GMP specific 5'-nucleotidase (lpg0095) in complex with phosphate ions from Legionella pneumophila, Northeast Structural Genomics Consortium Target LgR1==		==Crystal structure of cytosolic IMP-GMP specific 5'-nucleotidase (lpg0095) in complex with phosphate ions from Legionella pneumophila, Northeast Structural Genomics Consortium Target LgR1==
	<StructureSection load='4g63' size='340' side='right' caption='[[4g63]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='4g63' size='340' side='right' caption='[[4g63]], [[Resolution\|resolution]] 2.70Å' scene=''>
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	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2bde\|2bde]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2bde\|2bde]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">lpg0095 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272624 LEGPH])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">lpg0095 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272624 LEGPH])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4g63 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4g63 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4g63 RCSB], [http://www.ebi.ac.uk/pdbsum/4g63 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4g63 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4g63 OCA], [http://pdbe.org/4g63 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4g63 RCSB], [http://www.ebi.ac.uk/pdbsum/4g63 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4g63 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4g63" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 14:29, 4 January 2015

OCA — Sun, 04 Jan 2015 14:29:03 GMT

←Older revision		Revision as of 14:29, 4 January 2015
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4g63]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Legph Legph]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G63 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4G63 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4g63]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Legph Legph]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G63 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4G63 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2bde\|2bde]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2bde\|2bde]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">lpg0095 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272624 LEGPH])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">lpg0095 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272624 LEGPH])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4g63 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4g63 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4g63 RCSB], [http://www.ebi.ac.uk/pdbsum/4g63 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4g63 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4g63 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4g63 RCSB], [http://www.ebi.ac.uk/pdbsum/4g63 PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	Allosteric regulation and substrate activation in cytosolic nucleotidase II from Legionella pneumophila.,Srinivasan B, Forouhar F, Shukla A, Sampangi C, Kulkarni S, Abashidze M, Seetharaman J, Lew S, Mao L, Acton TB, Xiao R, Everett JK, Montelione GT, Tong L, Balaram H FEBS J. 2014 Jan 24. doi: 10.1111/febs.12727. PMID:24456211<ref>PMID:24456211</ref>		Allosteric regulation and substrate activation in cytosolic nucleotidase II from Legionella pneumophila.,Srinivasan B, Forouhar F, Shukla A, Sampangi C, Kulkarni S, Abashidze M, Seetharaman J, Lew S, Mao L, Acton TB, Xiao R, Everett JK, Montelione GT, Tong L, Balaram H FEBS J. 2014 Jan 24. doi: 10.1111/febs.12727. PMID:24456211<ref>PMID:24456211</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==
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	</StructureSection>		</StructureSection>
	[[Category: Legph]]		[[Category: Legph]]
-	[[Category: Abashidze, M.]]	+	[[Category: Abashidze, M]]
-	[[Category: Acton, T B.]]	+	[[Category: Acton, T B]]
-	[[Category: Ciccosanti, C.]]	+	[[Category: Ciccosanti, C]]
-	[[Category: Forouhar, F.]]	+	[[Category: Forouhar, F]]
-	[[Category: Ho, C K.]]	+	[[Category: Ho, C K]]
-	[[Category: Mao, L.]]	+	[[Category: Mao, L]]
-	[[Category: Montelione, G T.]]	+	[[Category: Montelione, G T]]
-	[[Category: ~~NESG, Northeast~~ Structural ~~Genomics Consortium.~~]]	+	[[Category: Structural genomic]]
-	[[Category: Seetharaman, J.]]	+	[[Category: Seetharaman, J]]
-	[[Category: Tong, L.]]	+	[[Category: Tong, L]]
-	[[Category: Xiao, R.]]	+	[[Category: Xiao, R]]
	[[Category: Alpha-beta protein]]		[[Category: Alpha-beta protein]]
	[[Category: Dna binding protein]]		[[Category: Dna binding protein]]
	[[Category: Had-like superfamily]]		[[Category: Had-like superfamily]]
	[[Category: Nesg]]		[[Category: Nesg]]
-	[[Category: Northeast structural genomics consortium]]
	[[Category: Psi-biology]]		[[Category: Psi-biology]]
-	[[Category: Structural genomic]]

OCA at 13:25, 18 May 2014

OCA — Sun, 18 May 2014 13:25:30 GMT

←Older revision		Revision as of 13:25, 18 May 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_4g63\| PDB=4g63 \| SCENE= }}~~	+	==Crystal structure of cytosolic IMP-GMP specific 5'-nucleotidase (lpg0095) in complex with phosphate ions from Legionella pneumophila, Northeast Structural Genomics Consortium Target LgR1==
-	===Crystal structure of cytosolic IMP-GMP specific 5'-nucleotidase (lpg0095) in complex with phosphate ions from Legionella pneumophila, Northeast Structural Genomics Consortium Target LgR1===	+	<StructureSection load='4g63' size='340' side='right' caption='[[4g63]], [[Resolution\|resolution]] 2.70Å' scene=''>
-	~~{{ABSTRACT_PUBMED_24456211}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4g63]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Legph Legph]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G63 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4G63 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2bde\|2bde]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">lpg0095 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272624 LEGPH])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4g63 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4g63 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4g63 RCSB], [http://www.ebi.ac.uk/pdbsum/4g63 PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Cytosolic nucleotidase II (cN-II) from Legionella pneumophila (Lp) catalyzes the hydrolysis of GMP and dGMP displaying sigmoidal curves, whereas catalysis of IMP hydrolysis displayed a biphasic curve in the initial rate versus substrate concentration plots. Allosteric modulators of mammalian cN-II did not activate LpcN-II although GTP, GDP and the substrate GMP were specific activators. Crystal structures of the tetrameric LpcN-II revealed an activator-binding site at the dimer interface. A double mutation in this allosteric-binding site abolished activation, confirming the structural observations. The substrate GMP acting as an activator, partitioning between the allosteric and active site, is the basis for the sigmoidicity of the initial velocity versus GMP concentration plot. The LpcN-II tetramer showed differences in subunit organization upon activator binding that are absent in the activator-bound human cN-II structure. This is the first observation of a structural change induced by activator binding in cN-II that may be the molecular mechanism for enzyme activation. DATABASE: The coordinates and structure factors reported in this paper have been submitted to the Protein Data Bank under the accession numbers 2BDE and 4G63. The accession number of GMP complexed LpcN-II is 4OHF.

-	~~==About this Structure==~~	+	Allosteric regulation and substrate activation in cytosolic nucleotidase II from Legionella pneumophila.,Srinivasan B, Forouhar F, Shukla A, Sampangi C, Kulkarni S, Abashidze M, Seetharaman J, Lew S, Mao L, Acton TB, Xiao R, Everett JK, Montelione GT, Tong L, Balaram H FEBS J. 2014 Jan 24. doi: 10.1111/febs.12727. PMID:24456211<ref>PMID:24456211</ref>
-	~~[[4g63]] is a 1 chain structure with sequence~~ from ~~[http://en~~.~~wikipedia~~.~~org/wiki/Legph Legph]~~. ~~Full crystallographic information is available from [http~~:/~~/oca~~.~~weizmann~~.~~ac.il~~/~~oca-bin/ocashort?id=4G63 OCA].~~	+

-	==~~Reference~~==	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group="xtra">PMID:024456211</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Legph]]		[[Category: Legph]]
	[[Category: Abashidze, M.]]		[[Category: Abashidze, M.]]

OCA at 07:35, 2 April 2014

OCA — Wed, 02 Apr 2014 07:35:18 GMT

←Older revision		Revision as of 07:35, 2 April 2014
Line 1:		Line 1:
-	[[Image:4g63.jpg\|left\|200px]]
-
	{{STRUCTURE_4g63\| PDB=4g63 \| SCENE= }}		{{STRUCTURE_4g63\| PDB=4g63 \| SCENE= }}
-
	===Crystal structure of cytosolic IMP-GMP specific 5'-nucleotidase (lpg0095) in complex with phosphate ions from Legionella pneumophila, Northeast Structural Genomics Consortium Target LgR1===		===Crystal structure of cytosolic IMP-GMP specific 5'-nucleotidase (lpg0095) in complex with phosphate ions from Legionella pneumophila, Northeast Structural Genomics Consortium Target LgR1===
		+	{{ABSTRACT_PUBMED_24456211}}

		+	==About this Structure==
		+	[[4g63]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Legph Legph]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G63 OCA].

-	==~~About this Structure~~==	+	==Reference==
-	~~[[4g63]] is a 1 chain structure. Full crystallographic information is available from [http~~://~~oca.weizmann.ac.il~~/~~oca-bin/ocashort?id=4G63 OCA~~].	+	<ref group="xtra">PMID:024456211</ref><references group="xtra"/><references/>
		+	[[Category: Legph]]
	[[Category: Abashidze, M.]]		[[Category: Abashidze, M.]]
	[[Category: Acton, T B.]]		[[Category: Acton, T B.]]

OCA at 07:28, 10 October 2012

OCA — Wed, 10 Oct 2012 07:28:38 GMT

←Older revision		Revision as of 07:28, 10 October 2012
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-	~~'''Unreleased structure'''~~	+	[[Image:4g63.jpg\|left\|200px]]

-	~~The entry~~ 4g63 ~~is ON HOLD~~	+	{{STRUCTURE_4g63\| PDB=4g63 \| SCENE= }}

-	~~Authors: Forouhar, F., Abashidze, M., Seetharaman, J., Ho, C.K., Ciccosanti, C., Mao, L., Xiao, R., Acton, T.B., Montelione, G.T., Tong, L.~~, Northeast Structural Genomics Consortium ~~(NESG)~~	+	===Crystal structure of cytosolic IMP-GMP specific 5'-nucleotidase (lpg0095) in complex with phosphate ions from Legionella pneumophila, Northeast Structural Genomics Consortium Target LgR1===

-	~~Description: Crystal~~ structure ~~of cytosolic IMP-GMP specific 5'-nucleotidase (lpg0095) in complex with phosphate ions~~ from ~~Legionella pneumophila~~, Northeast Structural Genomics Consortium ~~Target LgR1~~	+
		+	==About this Structure==
		+	[[4g63]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G63 OCA].
		+	[[Category: Abashidze, M.]]
		+	[[Category: Acton, T B.]]
		+	[[Category: Ciccosanti, C.]]
		+	[[Category: Forouhar, F.]]
		+	[[Category: Ho, C K.]]
		+	[[Category: Mao, L.]]
		+	[[Category: Montelione, G T.]]
		+	[[Category: NESG, Northeast Structural Genomics Consortium.]]
		+	[[Category: Seetharaman, J.]]
		+	[[Category: Tong, L.]]
		+	[[Category: Xiao, R.]]
		+	[[Category: Alpha-beta protein]]
		+	[[Category: Dna binding protein]]
		+	[[Category: Had-like superfamily]]
		+	[[Category: Nesg]]
		+	[[Category: Northeast structural genomics consortium]]
		+	[[Category: Psi-biology]]
		+	[[Category: Structural genomic]]

OCA: Protected "4g63" [edit=sysop:move=sysop]

OCA — Wed, 25 Jul 2012 06:53:45 GMT

Protected "4g63" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:53, 25 July 2012

OCA: New page: '''Unreleased structure''' The entry 4g63 is ON HOLD Authors: Forouhar, F., Abashidze, M., Seetharaman, J., Ho, C.K., Ciccosanti, C., Mao, L., Xiao, R., Acton, T.B., Montelione, G.T., T...

OCA — Wed, 25 Jul 2012 06:53:44 GMT

New page: '''Unreleased structure''' The entry 4g63 is ON HOLD Authors: Forouhar, F., Abashidze, M., Seetharaman, J., Ho, C.K., Ciccosanti, C., Mao, L., Xiao, R., Acton, T.B., Montelione, G.T., T...

New page

'''Unreleased structure'''

The entry 4g63 is ON HOLD

Authors: Forouhar, F., Abashidze, M., Seetharaman, J., Ho, C.K., Ciccosanti, C., Mao, L., Xiao, R., Acton, T.B., Montelione, G.T., Tong, L., Northeast Structural Genomics Consortium (NESG)

Description: Crystal structure of cytosolic IMP-GMP specific 5'-nucleotidase (lpg0095) in complex with phosphate ions from Legionella pneumophila, Northeast Structural Genomics Consortium Target LgR1