4g6g - Revision history

OCA at 08:51, 20 March 2024

OCA — Wed, 20 Mar 2024 08:51:12 GMT

←Older revision		Revision as of 08:51, 20 March 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4g6g]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G6G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4G6G FirstGlance]. <br>		<table><tr><td colspan='2'>[[4g6g]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G6G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4G6G FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TRT:FRAGMENT+OF+TRITON+X-100'>TRT</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.39Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TRT:FRAGMENT+OF+TRITON+X-100'>TRT</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4g6g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4g6g OCA], [https://pdbe.org/4g6g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4g6g RCSB], [https://www.ebi.ac.uk/pdbsum/4g6g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4g6g ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4g6g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4g6g OCA], [https://pdbe.org/4g6g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4g6g RCSB], [https://www.ebi.ac.uk/pdbsum/4g6g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4g6g ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/NDI1_YEAST NDI1_YEAST] Catalyzes the oxidation of NADH generated inside the Mitochondrion.		[https://www.uniprot.org/uniprot/NDI1_YEAST NDI1_YEAST] Catalyzes the oxidation of NADH generated inside the Mitochondrion.
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The single-component type-II NADH dehydrogenases (NDH-2s) serve as alternatives to the multisubunit respiratory complex I (type-I NADH dehydrogenase (NDH-1), also called NADH:ubiquinone oxidoreductase; EC 1.6.5.3) in catalysing electron transfer from NADH to ubiquinone in the mitochondrial respiratory chain. The yeast NDH-2 (Ndi1) oxidizes NADH on the matrix side and reduces ubiquinone to maintain mitochondrial NADH/NAD(+) homeostasis. Ndi1 is a potential therapeutic agent for human diseases caused by complex I defects, particularly Parkinson's disease, because its expression restores the mitochondrial activity in animals with complex I deficiency. NDH-2s in pathogenic microorganisms are viable targets for new antibiotics. Here we solve the crystal structures of Ndi1 in its substrate-free, NADH-, ubiquinone- and NADH-ubiquinone-bound states, to help understand the catalytic mechanism of NDH-2s. We find that Ndi1 homodimerization through its carboxy-terminal domain is critical for its catalytic activity and membrane targeting. The structures reveal two ubiquinone-binding sites (UQ(I) and UQ(II)) in Ndi1. NADH and UQ(I) can bind to Ndi1 simultaneously to form a substrate-protein complex. We propose that UQ(I) interacts with FAD to act as an intermediate for electron transfer, and that NADH transfers electrons through this FAD-UQ(I) complex to UQ(II). Together our data reveal the regulatory and catalytic mechanisms of Ndi1 and may facilitate the development or targeting of NDH-2s for potential therapeutic applications.
-
-	Structural insight into the type-II mitochondrial NADH dehydrogenases.,Feng Y, Li W, Li J, Wang J, Ge J, Xu D, Liu Y, Wu K, Zeng Q, Wu JW, Tian C, Zhou B, Yang M Nature. 2012 Nov 15;491(7424):478-82. doi: 10.1038/nature11541. Epub 2012 Oct 21. PMID:23086143<ref>PMID:23086143</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 4g6g" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 20:26, 19 October 2022

OCA — Wed, 19 Oct 2022 20:26:42 GMT

←Older revision		Revision as of 20:26, 19 October 2022
Line 1:		Line 1:

	==Crystal structure of NDH with TRT==		==Crystal structure of NDH with TRT==
-	<StructureSection load='4g6g' size='340' side='right' caption='[[4g6g]], [[Resolution\|resolution]] 2.39Å' scene=''>	+	<StructureSection load='4g6g' size='340' side='right'caption='[[4g6g]], [[Resolution\|resolution]] 2.39Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4g6g]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Baker's_yeast Baker's yeast~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G6G OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4G6G FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4g6g]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G6G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4G6G FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TRT:FRAGMENT+OF+TRITON+X-100'>TRT</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TRT:FRAGMENT+OF+TRITON+X-100'>TRT</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4g6h\|4g6h]], [[4g73\|4g73]], [[4g74\|4g74]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4g6g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4g6g OCA], [https://pdbe.org/4g6g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4g6g RCSB], [https://www.ebi.ac.uk/pdbsum/4g6g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4g6g ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">NDI1, YML120C, YM7056.06C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/NADH:ubiquinone_reductase_(non-electrogenic) NADH:ubiquinone reductase (non-electrogenic)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.6.5.9 1.6.5.9] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4g6g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4g6g OCA], [~~http~~://pdbe.org/4g6g PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4g6g RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4g6g PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4g6g ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/NDI1_YEAST NDI1_YEAST]] Catalyzes the oxidation of NADH generated inside the Mitochondrion.	+	[https://www.uniprot.org/uniprot/NDI1_YEAST NDI1_YEAST] Catalyzes the oxidation of NADH generated inside the Mitochondrion.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 25:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Baker's yeast~~]]	+	[[Category: Large Structures]]
-	[[Category: Feng, Y]]	+	[[Category: Saccharomyces cerevisiae S288C]]
-	[[Category: Ge, J]]	+	[[Category: Feng Y]]
-	[[Category: Li, W]]	+	[[Category: Ge J]]
-	[[Category: Yang, M]]	+	[[Category: Li W]]
-	~~[[Category: Electron transfer]]~~	+	[[Category: Yang M]]
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: Rossmann fold]]~~	+
-	~~[[Category: Trt~~]]	+

OCA at 17:49, 4 August 2016

OCA — Thu, 04 Aug 2016 17:49:29 GMT

←Older revision		Revision as of 17:49, 4 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of NDH with TRT==		==Crystal structure of NDH with TRT==
	<StructureSection load='4g6g' size='340' side='right' caption='[[4g6g]], [[Resolution\|resolution]] 2.39Å' scene=''>		<StructureSection load='4g6g' size='340' side='right' caption='[[4g6g]], [[Resolution\|resolution]] 2.39Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4g6g]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Saccharomyces_cerevisiae_s288c Saccharomyces cerevisiae s288c~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G6G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4G6G FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4g6g]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G6G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4G6G FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TRT:FRAGMENT+OF+TRITON+X-100'>TRT</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TRT:FRAGMENT+OF+TRITON+X-100'>TRT</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4g6h\|4g6h]], [[4g73\|4g73]], [[4g74\|4g74]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4g6h\|4g6h]], [[4g73\|4g73]], [[4g74\|4g74]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">NDI1, YML120C, YM7056.06C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 ~~Saccharomyces cerevisiae S288c~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">NDI1, YML120C, YM7056.06C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/NADH:ubiquinone_reductase_(non-electrogenic) NADH:ubiquinone reductase (non-electrogenic)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.6.5.9 1.6.5.9] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/NADH:ubiquinone_reductase_(non-electrogenic) NADH:ubiquinone reductase (non-electrogenic)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.6.5.9 1.6.5.9] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4g6g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4g6g OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4g6g RCSB], [http://www.ebi.ac.uk/pdbsum/4g6g PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4g6g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4g6g OCA], [http://pdbe.org/4g6g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4g6g RCSB], [http://www.ebi.ac.uk/pdbsum/4g6g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4g6g ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 19:		Line 20:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4g6g" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Saccharomyces cerevisiae s288c~~]]	+	[[Category: Baker's yeast]]
	[[Category: Feng, Y]]		[[Category: Feng, Y]]
	[[Category: Ge, J]]		[[Category: Ge, J]]

OCA at 17:49, 24 December 2014

OCA — Wed, 24 Dec 2014 17:49:25 GMT

←Older revision		Revision as of 17:49, 24 December 2014
Line 9:		Line 9:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4g6g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4g6g OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4g6g RCSB], [http://www.ebi.ac.uk/pdbsum/4g6g PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4g6g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4g6g OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4g6g RCSB], [http://www.ebi.ac.uk/pdbsum/4g6g PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/NDI1_YEAST NDI1_YEAST]] Catalyzes the oxidation of NADH generated inside the Mitochondrion.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 09:44, 10 December 2014

OCA — Wed, 10 Dec 2014 09:44:59 GMT

←Older revision		Revision as of 09:44, 10 December 2014
Line 1:		Line 1:
-	[[~~Image:~~4g6g.~~png~~\|~~left~~\|~~200px~~]]	+	==Crystal structure of NDH with TRT==
		+	<StructureSection load='4g6g' size='340' side='right' caption='[[4g6g]], [[Resolution\|resolution]] 2.39Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4g6g]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_s288c Saccharomyces cerevisiae s288c]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G6G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4G6G FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TRT:FRAGMENT+OF+TRITON+X-100'>TRT</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4g6h\|4g6h]], [[4g73\|4g73]], [[4g74\|4g74]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">NDI1, YML120C, YM7056.06C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Saccharomyces cerevisiae S288c])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/NADH:ubiquinone_reductase_(non-electrogenic) NADH:ubiquinone reductase (non-electrogenic)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.6.5.9 1.6.5.9] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4g6g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4g6g OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4g6g RCSB], [http://www.ebi.ac.uk/pdbsum/4g6g PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The single-component type-II NADH dehydrogenases (NDH-2s) serve as alternatives to the multisubunit respiratory complex I (type-I NADH dehydrogenase (NDH-1), also called NADH:ubiquinone oxidoreductase; EC 1.6.5.3) in catalysing electron transfer from NADH to ubiquinone in the mitochondrial respiratory chain. The yeast NDH-2 (Ndi1) oxidizes NADH on the matrix side and reduces ubiquinone to maintain mitochondrial NADH/NAD(+) homeostasis. Ndi1 is a potential therapeutic agent for human diseases caused by complex I defects, particularly Parkinson's disease, because its expression restores the mitochondrial activity in animals with complex I deficiency. NDH-2s in pathogenic microorganisms are viable targets for new antibiotics. Here we solve the crystal structures of Ndi1 in its substrate-free, NADH-, ubiquinone- and NADH-ubiquinone-bound states, to help understand the catalytic mechanism of NDH-2s. We find that Ndi1 homodimerization through its carboxy-terminal domain is critical for its catalytic activity and membrane targeting. The structures reveal two ubiquinone-binding sites (UQ(I) and UQ(II)) in Ndi1. NADH and UQ(I) can bind to Ndi1 simultaneously to form a substrate-protein complex. We propose that UQ(I) interacts with FAD to act as an intermediate for electron transfer, and that NADH transfers electrons through this FAD-UQ(I) complex to UQ(II). Together our data reveal the regulatory and catalytic mechanisms of Ndi1 and may facilitate the development or targeting of NDH-2s for potential therapeutic applications.

-	~~{{STRUCTURE_4g6g\| PDB=4g6g \| SCENE= }}~~	+	Structural insight into the type-II mitochondrial NADH dehydrogenases.,Feng Y, Li W, Li J, Wang J, Ge J, Xu D, Liu Y, Wu K, Zeng Q, Wu JW, Tian C, Zhou B, Yang M Nature. 2012 Nov 15;491(7424):478-82. doi: 10.1038/nature11541. Epub 2012 Oct 21. PMID:23086143<ref>PMID:23086143</ref>

-	~~===Crystal structure~~ of ~~NDH with TRT===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_23086143}}~~	+	== References ==
-		+	<references/>
-	==~~About this Structure~~==	+	__TOC__
-	~~[[4g6g]] is a 2 chain structure with sequence from [http:~~//~~en.wikipedia.org/wiki/Saccharomyces_cerevisiae_s288c Saccharomyces cerevisiae s288c]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G6G OCA].~~	+	</StructureSection>
	[[Category: Saccharomyces cerevisiae s288c]]		[[Category: Saccharomyces cerevisiae s288c]]
-	[[Category: Feng, Y.]]	+	[[Category: Feng, Y]]
-	[[Category: Ge, J.]]	+	[[Category: Ge, J]]
-	[[Category: Li, W.]]	+	[[Category: Li, W]]
-	[[Category: Yang, M.]]	+	[[Category: Yang, M]]
	[[Category: Electron transfer]]		[[Category: Electron transfer]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]
	[[Category: Rossmann fold]]		[[Category: Rossmann fold]]
	[[Category: Trt]]		[[Category: Trt]]

OCA at 09:55, 5 December 2012

OCA — Wed, 05 Dec 2012 09:55:40 GMT

←Older revision		Revision as of 09:55, 5 December 2012
Line 1:		Line 1:
-	[[Image:4g6g.~~jpg~~\|left\|200px]]	+	[[Image:4g6g.png\|left\|200px]]

	{{STRUCTURE_4g6g\| PDB=4g6g \| SCENE= }}		{{STRUCTURE_4g6g\| PDB=4g6g \| SCENE= }}
Line 5:		Line 5:
	===Crystal structure of NDH with TRT===		===Crystal structure of NDH with TRT===

		+	{{ABSTRACT_PUBMED_23086143}}

	==About this Structure==		==About this Structure==

OCA at 07:45, 24 October 2012

OCA — Wed, 24 Oct 2012 07:45:29 GMT

←Older revision		Revision as of 07:45, 24 October 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:4g6g.jpg\|left\|200px]]

-	~~The entry~~ 4g6g ~~is ON HOLD~~ ~~until Paper Publication~~	+	{{STRUCTURE_4g6g\| PDB=4g6g \| SCENE= }}

-	~~Authors: Li, W., Feng, Y., Ge, J., Yang, M.~~	+	===Crystal structure of NDH with TRT===

-	~~Description: Crystal~~ structure ~~of NDH~~ with ~~TRT~~	+
		+	==About this Structure==
		+	[[4g6g]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_s288c Saccharomyces cerevisiae s288c]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G6G OCA].
		+	[[Category: Saccharomyces cerevisiae s288c]]
		+	[[Category: Feng, Y.]]
		+	[[Category: Ge, J.]]
		+	[[Category: Li, W.]]
		+	[[Category: Yang, M.]]
		+	[[Category: Electron transfer]]
		+	[[Category: Oxidoreductase]]
		+	[[Category: Rossmann fold]]
		+	[[Category: Trt]]

OCA at 05:31, 22 August 2012

OCA — Wed, 22 Aug 2012 05:31:15 GMT

←Older revision		Revision as of 05:31, 22 August 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4g6g is ON HOLD	+	The entry 4g6g is ON HOLD until Paper Publication

	Authors: Li, W., Feng, Y., Ge, J., Yang, M.		Authors: Li, W., Feng, Y., Ge, J., Yang, M.

	Description: Crystal structure of NDH with TRT		Description: Crystal structure of NDH with TRT

OCA at 05:22, 15 August 2012

OCA — Wed, 15 Aug 2012 05:22:36 GMT

←Older revision		Revision as of 05:22, 15 August 2012
Line 3:		Line 3:
	The entry 4g6g is ON HOLD		The entry 4g6g is ON HOLD

-	Authors: Li,W, Feng,Y, Ge,J, Yang,M	+	Authors: Li, W., Feng, Y., Ge, J., Yang, M.

	Description: Crystal structure of NDH with TRT		Description: Crystal structure of NDH with TRT

OCA: Protected "4g6g" [edit=sysop:move=sysop]

OCA — Wed, 25 Jul 2012 06:54:07 GMT

Protected "4g6g" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:54, 25 July 2012