4g7n - Revision history

OCA at 20:29, 19 October 2022

OCA — Wed, 19 Oct 2022 20:29:00 GMT

←Older revision		Revision as of 20:29, 19 October 2022
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	==The Structure of the Plk4 Cryptic Polo Box Reveals Two Tandem Polo Boxes Required for Centriole Duplication==		==The Structure of the Plk4 Cryptic Polo Box Reveals Two Tandem Polo Boxes Required for Centriole Duplication==
-	<StructureSection load='4g7n' size='340' side='right' caption='[[4g7n]], [[Resolution\|resolution]] 2.30Å' scene=''>	+	<StructureSection load='4g7n' size='340' side='right'caption='[[4g7n]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4g7n]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Drome Drome~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G7N OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4G7N FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4g7n]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G7N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4G7N FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CG7186, Plk4, SAK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME])</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4g7n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4g7n OCA], [https://pdbe.org/4g7n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4g7n RCSB], [https://www.ebi.ac.uk/pdbsum/4g7n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4g7n ProSAT]</span></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Polo_kinase Polo kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.21 2.7.11.21] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4g7n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4g7n OCA], [~~http~~://pdbe.org/4g7n PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4g7n RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4g7n PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4g7n ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/PLK4_DROME PLK4_DROME]] Serine/threonine-protein kinase that plays a central role in centriole duplication. Able to trigger procentriole formation on the surface of the mother centriole cylinder, using mother centriole as a platform, leading to the recruitment of centriole biogenesis proteins such as Sas-6. When overexpressed, it is able to induce centrosome amplification through the simultaneous generation of multiple procentrioles adjoining each parental centriole during S phase. Centrosome amplification following overexpression can initiate tumorigenesis, highlighting the importance of centrosome regulation in cancers.<ref>PMID:16326102</ref> <ref>PMID:17463247</ref> <ref>PMID:18555779</ref>	+	[https://www.uniprot.org/uniprot/PLK4_DROME PLK4_DROME] Serine/threonine-protein kinase that plays a central role in centriole duplication. Able to trigger procentriole formation on the surface of the mother centriole cylinder, using mother centriole as a platform, leading to the recruitment of centriole biogenesis proteins such as Sas-6. When overexpressed, it is able to induce centrosome amplification through the simultaneous generation of multiple procentrioles adjoining each parental centriole during S phase. Centrosome amplification following overexpression can initiate tumorigenesis, highlighting the importance of centrosome regulation in cancers.<ref>PMID:16326102</ref> <ref>PMID:17463247</ref> <ref>PMID:18555779</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	==See Also==		==See Also==
-	*[[Serine/threonine protein kinase\|Serine/threonine protein kinase]]	+	*[[Serine/threonine protein kinase 3D structures\|Serine/threonine protein kinase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Drome~~]]	+	[[Category: Drosophila melanogaster]]
-	[[Category: ~~Polo kinase~~]]	+	[[Category: Large Structures]]
-	[[Category: Slep~~, K C~~]]	+	[[Category: Slep KC]]
-	[[Category: Slevin~~, L K]]~~	+	[[Category: Slevin LK]]
-	~~[[Category: Asterless]]~~	+
-	~~[[Category: Centriole]]~~	+
-	~~[[Category: Kinase targeting and regulation]]~~	+
-	~~[[Category: Polo box]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 21:07, 4 August 2016

OCA — Thu, 04 Aug 2016 21:07:53 GMT

←Older revision		Revision as of 21:07, 4 August 2016
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		+
	==The Structure of the Plk4 Cryptic Polo Box Reveals Two Tandem Polo Boxes Required for Centriole Duplication==		==The Structure of the Plk4 Cryptic Polo Box Reveals Two Tandem Polo Boxes Required for Centriole Duplication==
	<StructureSection load='4g7n' size='340' side='right' caption='[[4g7n]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='4g7n' size='340' side='right' caption='[[4g7n]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4g7n]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Drosophila_melanogaster Drosophila melanogaster~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G7N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4G7N FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4g7n]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Drome Drome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G7N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4G7N FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CG7186, Plk4, SAK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 ~~Drosophila melanogaster~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CG7186, Plk4, SAK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Polo_kinase Polo kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.21 2.7.11.21] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Polo_kinase Polo kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.21 2.7.11.21] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4g7n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4g7n OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4g7n RCSB], [http://www.ebi.ac.uk/pdbsum/4g7n PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4g7n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4g7n OCA], [http://pdbe.org/4g7n PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4g7n RCSB], [http://www.ebi.ac.uk/pdbsum/4g7n PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4g7n ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4g7n" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Serine/threonine protein kinase\|Serine/threonine protein kinase]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Drosophila melanogaster~~]]	+	[[Category: Drome]]
	[[Category: Polo kinase]]		[[Category: Polo kinase]]
	[[Category: Slep, K C]]		[[Category: Slep, K C]]

OCA at 19:40, 24 December 2014

OCA — Wed, 24 Dec 2014 19:40:30 GMT

←Older revision		Revision as of 19:40, 24 December 2014
Line 1:		Line 1:
-	[[~~Image:~~4g7n.~~png~~\|~~left~~\|~~200px~~]]	+	==The Structure of the Plk4 Cryptic Polo Box Reveals Two Tandem Polo Boxes Required for Centriole Duplication==
		+	<StructureSection load='4g7n' size='340' side='right' caption='[[4g7n]], [[Resolution\|resolution]] 2.30Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4g7n]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G7N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4G7N FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CG7186, Plk4, SAK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 Drosophila melanogaster])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Polo_kinase Polo kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.21 2.7.11.21] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4g7n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4g7n OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4g7n RCSB], [http://www.ebi.ac.uk/pdbsum/4g7n PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/PLK4_DROME PLK4_DROME]] Serine/threonine-protein kinase that plays a central role in centriole duplication. Able to trigger procentriole formation on the surface of the mother centriole cylinder, using mother centriole as a platform, leading to the recruitment of centriole biogenesis proteins such as Sas-6. When overexpressed, it is able to induce centrosome amplification through the simultaneous generation of multiple procentrioles adjoining each parental centriole during S phase. Centrosome amplification following overexpression can initiate tumorigenesis, highlighting the importance of centrosome regulation in cancers.<ref>PMID:16326102</ref> <ref>PMID:17463247</ref> <ref>PMID:18555779</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Centrioles are key microtubule polarity determinants. Centriole duplication is tightly controlled to prevent cells from developing multipolar spindles, a situation that promotes chromosomal instability. A conserved component in the duplication pathway is Plk4, a polo kinase family member that localizes to centrioles in M/G1. To limit centriole duplication, Plk4 levels are controlled through trans-autophosphorylation that primes ubiquitination. In contrast to Plks 1-3, Plk4 possesses a unique central region called the "cryptic polo box." Here, we present the crystal structure of this region at 2.3 A resolution. Surprisingly, the structure reveals two tandem homodimerized polo boxes, PB1-PB2, that form a unique winged architecture. The full PB1-PB2 cassette is required for binding the centriolar protein Asterless as well as robust centriole targeting. Thus, with its C-terminal polo box (PB3), Plk4 has a triple polo box architecture that facilitates oligomerization, targeting, and promotes trans-autophosphorylation, limiting centriole duplication to once per cell cycle.

-	~~{{STRUCTURE_4g7n\| PDB=4g7n \| SCENE= }}~~	+	The Structure of the Plk4 Cryptic Polo Box Reveals Two Tandem Polo Boxes Required for Centriole Duplication.,Slevin LK, Nye J, Pinkerton DC, Buster DW, Rogers GC, Slep KC Structure. 2012 Sep 18. pii: S0969-2126(12)00328-0. doi:, 10.1016/j.str.2012.08.025. PMID:23000383<ref>PMID:23000383</ref>

-	~~===The Structure~~ of the ~~Plk4 Cryptic Polo Box Reveals Two Tandem Polo Boxes Required for Centriole Duplication===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_23000383}}~~	+	== References ==
-		+	<references/>
-	==~~About this Structure~~==	+	__TOC__
-	~~[[4g7n]] is a 2 chain structure with sequence from [http:~~//~~en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G7N OCA].~~	+	</StructureSection>
	[[Category: Drosophila melanogaster]]		[[Category: Drosophila melanogaster]]
	[[Category: Polo kinase]]		[[Category: Polo kinase]]
-	[[Category: Slep, K C.]]	+	[[Category: Slep, K C]]
-	[[Category: Slevin, L K.]]	+	[[Category: Slevin, L K]]
	[[Category: Asterless]]		[[Category: Asterless]]
	[[Category: Centriole]]		[[Category: Centriole]]

OCA at 21:33, 12 December 2012

OCA — Wed, 12 Dec 2012 21:33:38 GMT

←Older revision		Revision as of 21:33, 12 December 2012
Line 1:		Line 1:
-	[[Image:4g7n.~~jpg~~\|left\|200px]]	+	[[Image:4g7n.png\|left\|200px]]

	{{STRUCTURE_4g7n\| PDB=4g7n \| SCENE= }}		{{STRUCTURE_4g7n\| PDB=4g7n \| SCENE= }}
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[4g7n]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G7N OCA].	+	[[4g7n]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G7N OCA].
		+	[[Category: Drosophila melanogaster]]
	[[Category: Polo kinase]]		[[Category: Polo kinase]]
	[[Category: Slep, K C.]]		[[Category: Slep, K C.]]

OCA at 07:21, 10 October 2012

OCA — Wed, 10 Oct 2012 07:21:11 GMT

←Older revision		Revision as of 07:21, 10 October 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:4g7n.jpg\|left\|200px]]

-	~~The entry~~ 4g7n ~~is ON HOLD~~	+	{{STRUCTURE_4g7n\| PDB=4g7n \| SCENE= }}

-	~~Authors: Slep, K.C., Slevin, L.K.~~	+	===The Structure of the Plk4 Cryptic Polo Box Reveals Two Tandem Polo Boxes Required for Centriole Duplication===

-	~~Description: The~~ Structure ~~of the Plk4 Cryptic~~ Polo ~~Box Reveals Two Tandem Polo Boxes Required for~~ Centriole ~~Duplication~~	+	{{ABSTRACT_PUBMED_23000383}}
		+
		+	==About this Structure==
		+	[[4g7n]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G7N OCA].
		+	[[Category: Polo kinase]]
		+	[[Category: Slep, K C.]]
		+	[[Category: Slevin, L K.]]
		+	[[Category: Asterless]]
		+	[[Category: Centriole]]
		+	[[Category: Kinase targeting and regulation]]
		+	[[Category: Polo box]]
		+	[[Category: Transferase]]

OCA: Protected "4g7n" [edit=sysop:move=sysop]

OCA — Wed, 01 Aug 2012 05:29:49 GMT

Protected "4g7n" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:29, 1 August 2012

OCA: New page: '''Unreleased structure''' The entry 4g7n is ON HOLD Authors: Slep, K.C., Slevin, L.K. Description: The Structure of the Plk4 Cryptic Polo Box Reveals Two Tandem Polo Boxes Required fo...

OCA — Wed, 01 Aug 2012 05:29:48 GMT

New page: '''Unreleased structure''' The entry 4g7n is ON HOLD Authors: Slep, K.C., Slevin, L.K. Description: The Structure of the Plk4 Cryptic Polo Box Reveals Two Tandem Polo Boxes Required fo...

New page

'''Unreleased structure'''

The entry 4g7n is ON HOLD

Authors: Slep, K.C., Slevin, L.K.

Description: The Structure of the Plk4 Cryptic Polo Box Reveals Two Tandem Polo Boxes Required for Centriole Duplication