4g7w - Revision history

OCA at 20:29, 19 October 2022

OCA — Wed, 19 Oct 2022 20:29:10 GMT

←Older revision		Revision as of 20:29, 19 October 2022
Line 1:		Line 1:

	==Crystal structure of the N-terminal domain of the minor coat protein pIII from CTXphi==		==Crystal structure of the N-terminal domain of the minor coat protein pIII from CTXphi==
-	<StructureSection load='4g7w' size='340' side='right' caption='[[4g7w]], [[Resolution\|resolution]] 2.90Å' scene=''>	+	<StructureSection load='4g7w' size='340' side='right'caption='[[4g7w]], [[Resolution\|resolution]] 2.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4g7w]] is a 3 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Vibrio_cholera_cirs_101~~ Vibrio ~~cholera cirs 101~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G7W OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4G7W FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4g7w]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_cholerae_CIRS101 Vibrio cholerae CIRS101]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G7W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4G7W FirstGlance]. <br>
-	</td></tr><tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4g7x\|4g7x]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4g7w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4g7w OCA], [https://pdbe.org/4g7w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4g7w RCSB], [https://www.ebi.ac.uk/pdbsum/4g7w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4g7w ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">orfU, VCH_002198 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=661513 Vibrio cholera CIRS 101])</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4g7w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4g7w OCA], [~~http~~://pdbe.org/4g7w PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4g7w RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4g7w PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4g7w ProSAT]</span></td></tr>	+
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 22:		Line 20:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Vibrio cholera cirs 101~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Craig, L~~]]	+	[[Category: Vibrio cholerae CIRS101]]
-	[[Category: ~~Ford, C G~~]]	+	[[Category: Craig L]]
-	[[Category: ~~Kolappan, S~~]]	+	[[Category: Ford CG]]
-	[[Category: ~~Beta barrel]]~~	+	[[Category: Kolappan S]]
-	~~[[Category: Phage coat]]~~	+
-	~~[[Category: Protein binding]]~~	+
-	~~[[Category: Selenomethionine-substituted protein]]~~	+
-	~~[[Category: Tola binding domain~~]]	+

OCA at 19:53, 15 December 2016

OCA — Thu, 15 Dec 2016 19:53:59 GMT

←Older revision		Revision as of 19:53, 15 December 2016
Line 1:		Line 1:
		+
	==Crystal structure of the N-terminal domain of the minor coat protein pIII from CTXphi==		==Crystal structure of the N-terminal domain of the minor coat protein pIII from CTXphi==
	<StructureSection load='4g7w' size='340' side='right' caption='[[4g7w]], [[Resolution\|resolution]] 2.90Å' scene=''>		<StructureSection load='4g7w' size='340' side='right' caption='[[4g7w]], [[Resolution\|resolution]] 2.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4g7w]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Vibrio_cholerae_cirs101~~ Vibrio ~~cholerae cirs101~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G7W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4G7W FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4g7w]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Vibrio_cholera_cirs_101 Vibrio cholera cirs 101]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G7W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4G7W FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4g7x\|4g7x]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4g7x\|4g7x]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">orfU, VCH_002198 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=661513 Vibrio ~~cholerae CIRS101~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">orfU, VCH_002198 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=661513 Vibrio cholera CIRS 101])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4g7w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4g7w OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4g7w RCSB], [http://www.ebi.ac.uk/pdbsum/4g7w PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4g7w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4g7w OCA], [http://pdbe.org/4g7w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4g7w RCSB], [http://www.ebi.ac.uk/pdbsum/4g7w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4g7w ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4g7w" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Vibrio ~~cholerae cirs101~~]]	+	[[Category: Vibrio cholera cirs 101]]
	[[Category: Craig, L]]		[[Category: Craig, L]]
	[[Category: Ford, C G]]		[[Category: Ford, C G]]

OCA at 09:24, 15 February 2015

OCA — Sun, 15 Feb 2015 09:24:52 GMT

←Older revision		Revision as of 09:24, 15 February 2015
Line 1:		Line 1:
-	[[~~Image:~~4g7w.~~png~~\|~~left~~\|~~200px~~]]	+	==Crystal structure of the N-terminal domain of the minor coat protein pIII from CTXphi==
		+	<StructureSection load='4g7w' size='340' side='right' caption='[[4g7w]], [[Resolution\|resolution]] 2.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4g7w]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Vibrio_cholerae_cirs101 Vibrio cholerae cirs101]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G7W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4G7W FirstGlance]. <br>
		+	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4g7x\|4g7x]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">orfU, VCH_002198 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=661513 Vibrio cholerae CIRS101])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4g7w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4g7w OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4g7w RCSB], [http://www.ebi.ac.uk/pdbsum/4g7w PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Vibrio cholerae colonize the small intestine where they secrete cholera toxin, an ADP-ribosylating enzyme that is responsible for the volumous diarrhea characteristic of cholera disease. The genes encoding cholera toxin are located on the genome of the filamentous bacteriophage, CTXvarphi, which integrates as a prophage into the V. cholerae chromosome. CTXvarphi infection of V. cholerae requires the toxin coregulated pilus (TCP) and the periplasmic protein TolA. This infection process parallels that of Escherichia coli infection by the Ff family of filamentous coliphage. Here, we demonstrate a direct interaction between the N-terminal domain of the CTXvarphi minor coat protein pIII (pIII-N1) and the C-terminal domain of TolA (TolA-C) and present x-ray crystal structures of pIII-N1 alone and in complex with TolA-C. The structures of CTXvarphi pIII-N1 and V. cholerae TolA-C are similar to coliphage pIII-N1 and E. coli TolA-C, respectively, yet these proteins bind via a distinct interface that in E. coli TolA corresponds to a colicin binding site. Our data suggest that the TolA binding site on pIII-N1 of CTXvarphi is accessible in the native pIII protein. This contrasts with the Ff family phage, where the TolA binding site on pIII is blocked and requires a pilus-induced unfolding event to become exposed. We propose that CTXvarphi pIII accesses the periplasmic TolA through retraction of TCP, which bring the phage through the outer membrane pilus secretin channel. These data help to explain the process by which CTXvarphi converts a harmless marine microbe into a deadly human pathogen.

-	{~~{STRUCTURE_4g7w\| PDB=4g7w \| SCENE= }~~}	+	Crystal Structures of CTX{varphi} pIII Unbound and in Complex with Vibrio cholerae TolA Reveal Novel Interaction Interfaces.,Ford CG, Kolappan S, Phan HT, Winther-Larsen HC, Craig L J Biol Chem. 2012 Aug 31. PMID:22942280<ref>PMID:22942280</ref>

-	~~===Crystal structure~~ of the ~~N-terminal domain~~ of ~~the minor coat protein pIII from CTXphi===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_22942280}}~~	+	== References ==
-		+	<references/>
-	==~~About this Structure~~==	+	__TOC__
-	~~[[4g7w]] is a 3 chain structure with sequence from [http:~~//~~en.wikipedia.org/wiki/Vibrio_cholerae_cirs101 Vibrio cholerae cirs101]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G7W OCA].~~	+	</StructureSection>
	[[Category: Vibrio cholerae cirs101]]		[[Category: Vibrio cholerae cirs101]]
-	[[Category: Craig, L.]]	+	[[Category: Craig, L]]
-	[[Category: Ford, C G.]]	+	[[Category: Ford, C G]]
-	[[Category: Kolappan, S.]]	+	[[Category: Kolappan, S]]
	[[Category: Beta barrel]]		[[Category: Beta barrel]]
	[[Category: Phage coat]]		[[Category: Phage coat]]

OCA at 10:38, 14 November 2012

OCA — Wed, 14 Nov 2012 10:38:52 GMT

←Older revision		Revision as of 10:38, 14 November 2012
Line 5:		Line 5:
	===Crystal structure of the N-terminal domain of the minor coat protein pIII from CTXphi===		===Crystal structure of the N-terminal domain of the minor coat protein pIII from CTXphi===

		+	{{ABSTRACT_PUBMED_22942280}}

	==About this Structure==		==About this Structure==

OCA at 14:16, 8 November 2012

OCA — Thu, 08 Nov 2012 14:16:35 GMT

←Older revision		Revision as of 14:16, 8 November 2012
Line 5:		Line 5:
	===Crystal structure of the N-terminal domain of the minor coat protein pIII from CTXphi===		===Crystal structure of the N-terminal domain of the minor coat protein pIII from CTXphi===

-	{{ABSTRACT_PUBMED_22942280}}

	==About this Structure==		==About this Structure==
-	[[4g7w]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Vibrio_cholerae~~ Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G7W OCA].	+	[[4g7w]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Vibrio_cholerae_cirs101 Vibrio cholerae cirs101]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G7W OCA].
-	[[Category: Vibrio cholerae]]	+	[[Category: Vibrio cholerae cirs101]]
	[[Category: Craig, L.]]		[[Category: Craig, L.]]
	[[Category: Ford, C G.]]		[[Category: Ford, C G.]]

OCA at 06:07, 19 September 2012

OCA — Wed, 19 Sep 2012 06:07:15 GMT

←Older revision		Revision as of 06:07, 19 September 2012
Line 1:		Line 1:
-	[[Image:4g7w.~~jpg~~\|left\|200px]]	+	[[Image:4g7w.png\|left\|200px]]

	{{STRUCTURE_4g7w\| PDB=4g7w \| SCENE= }}		{{STRUCTURE_4g7w\| PDB=4g7w \| SCENE= }}
Line 5:		Line 5:
	===Crystal structure of the N-terminal domain of the minor coat protein pIII from CTXphi===		===Crystal structure of the N-terminal domain of the minor coat protein pIII from CTXphi===

		+	{{ABSTRACT_PUBMED_22942280}}

	==About this Structure==		==About this Structure==

OCA at 05:29, 29 August 2012

OCA — Wed, 29 Aug 2012 05:29:31 GMT

←Older revision		Revision as of 05:29, 29 August 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:4g7w.jpg\|left\|200px]]

-	~~The entry~~ 4g7w ~~is ON HOLD~~	+	{{STRUCTURE_4g7w\| PDB=4g7w \| SCENE= }}

-	~~Authors: Kolappan, S., Ford, C.G., Craig, L.~~	+	===Crystal structure of the N-terminal domain of the minor coat protein pIII from CTXphi===

-	~~Description: Crystal~~ structure ~~of the N~~-~~terminal domain of the minor~~ coat protein ~~pIII from CTXphi~~	+
		+	==About this Structure==
		+	[[4g7w]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G7W OCA].
		+	[[Category: Vibrio cholerae]]
		+	[[Category: Craig, L.]]
		+	[[Category: Ford, C G.]]
		+	[[Category: Kolappan, S.]]
		+	[[Category: Beta barrel]]
		+	[[Category: Phage coat]]
		+	[[Category: Protein binding]]
		+	[[Category: Selenomethionine-substituted protein]]
		+	[[Category: Tola binding domain]]

OCA: Protected "4g7w" [edit=sysop:move=sysop]

OCA — Wed, 01 Aug 2012 05:30:04 GMT

Protected "4g7w" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:30, 1 August 2012

OCA: New page: '''Unreleased structure''' The entry 4g7w is ON HOLD Authors: Kolappan, S., Ford, C.G., Craig, L. Description: Crystal structure of the N-terminal domain of the minor coat protein pIII...

OCA — Wed, 01 Aug 2012 05:30:03 GMT

New page: '''Unreleased structure''' The entry 4g7w is ON HOLD Authors: Kolappan, S., Ford, C.G., Craig, L. Description: Crystal structure of the N-terminal domain of the minor coat protein pIII...

New page

'''Unreleased structure'''

The entry 4g7w is ON HOLD

Authors: Kolappan, S., Ford, C.G., Craig, L.

Description: Crystal structure of the N-terminal domain of the minor coat protein pIII from CTXphi