4g8x - Revision history

OCA at 06:43, 26 October 2022

OCA — Wed, 26 Oct 2022 06:43:06 GMT

←Older revision		Revision as of 06:43, 26 October 2022
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	==G1 ORF67 / Staphyloccus aureus sigmaA domain 4 complex==		==G1 ORF67 / Staphyloccus aureus sigmaA domain 4 complex==
-	<StructureSection load='4g8x' size='340' side='right' caption='[[4g8x]], [[Resolution\|resolution]] 3.00Å' scene=''>	+	<StructureSection load='4g8x' size='340' side='right'caption='[[4g8x]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4g8x]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Bacteriophage_g1 Bacteriophage g1~~] and [~~http~~://en.wikipedia.org/wiki/~~Staa8 Staa8~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G8X OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4G8X FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4g8x]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_NCTC_8325 Staphylococcus aureus subsp. aureus NCTC 8325] and [https://en.wikipedia.org/wiki/Staphylococcus_virus_G1 Staphylococcus virus G1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G8X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4G8X FirstGlance]. <br>
-	</td></tr><tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4g6d\|4g6d]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4g8x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4g8x OCA], [https://pdbe.org/4g8x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4g8x RCSB], [https://www.ebi.ac.uk/pdbsum/4g8x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4g8x ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">plaC, rpoD, SAOUHSC_01662, sigA, Sigma A domain 4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=93061 STAA8]), ORF67 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=292029 Bacteriophage G1])</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4g8x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4g8x OCA], [~~http~~://pdbe.org/4g8x PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4g8x RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4g8x PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4g8x ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/SIGA_STAA8 SIGA_STAA8]] Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is the primary sigma factor during exponential growth.	+	[https://www.uniprot.org/uniprot/SIGA_STAA8 SIGA_STAA8] Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is the primary sigma factor during exponential growth.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 20:

	==See Also==		==See Also==
-	*[[Sigma factor\|Sigma factor]]	+	*[[Sigma factor 3D structures\|Sigma factor 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacteriophage g1~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Staa8~~]]	+	[[Category: Staphylococcus aureus subsp. aureus NCTC 8325]]
-	[[Category: Darst~~, S A~~]]	+	[[Category: Staphylococcus virus G1]]
-	[[Category: Hochschild, A]]	+	[[Category: Darst SA]]
-	[[Category: Montero-Diaz~~, C M~~]]	+	[[Category: Hochschild A]]
-	[[Category: Osmundson~~, J S]]~~	+	[[Category: Montero-Diaz CM]]
-	~~[[Category: Dna binding protein]]~~	+	[[Category: Osmundson JS]]
-	~~[[Category: Rnap binding protein~~]]	+

OCA at 14:04, 5 August 2016

OCA — Fri, 05 Aug 2016 14:04:34 GMT

←Older revision		Revision as of 14:04, 5 August 2016
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		+
	==G1 ORF67 / Staphyloccus aureus sigmaA domain 4 complex==		==G1 ORF67 / Staphyloccus aureus sigmaA domain 4 complex==
	<StructureSection load='4g8x' size='340' side='right' caption='[[4g8x]], [[Resolution\|resolution]] 3.00Å' scene=''>		<StructureSection load='4g8x' size='340' side='right' caption='[[4g8x]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4g8x]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Staphylococcus_aureus_subsp._aureus_nctc_8325 Staphylococcus aureus subsp. aureus nctc 8325~~] and [http://en.wikipedia.org/wiki/~~Staphylococcus_phage_g1 Staphylococcus phage g1~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G8X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4G8X FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4g8x]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacteriophage_g1 Bacteriophage g1] and [http://en.wikipedia.org/wiki/Staa8 Staa8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G8X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4G8X FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4g6d\|4g6d]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4g6d\|4g6d]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">plaC, rpoD, SAOUHSC_01662, sigA, Sigma A domain 4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=93061 ~~Staphylococcus aureus subsp. aureus NCTC 8325~~]), ORF67 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=292029 ~~Staphylococcus phage~~ G1])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">plaC, rpoD, SAOUHSC_01662, sigA, Sigma A domain 4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=93061 STAA8]), ORF67 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=292029 Bacteriophage G1])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4g8x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4g8x OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4g8x RCSB], [http://www.ebi.ac.uk/pdbsum/4g8x PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4g8x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4g8x OCA], [http://pdbe.org/4g8x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4g8x RCSB], [http://www.ebi.ac.uk/pdbsum/4g8x PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4g8x ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4g8x" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 25:		Line 27:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Staphylococcus aureus subsp. aureus nctc 8325~~]]	+	[[Category: Bacteriophage g1]]
-	[[Category: ~~Staphylococcus phage g1~~]]	+	[[Category: Staa8]]
	[[Category: Darst, S A]]		[[Category: Darst, S A]]
	[[Category: Hochschild, A]]		[[Category: Hochschild, A]]

OCA at 03:06, 25 December 2014

OCA — Thu, 25 Dec 2014 03:06:34 GMT

←Older revision		Revision as of 03:06, 25 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_4g8x\| PDB~~=4g8x \| ~~SCENE~~= }}	+	==G1 ORF67 / Staphyloccus aureus sigmaA domain 4 complex==
-	===~~G1 ORF67~~ / ~~Staphyloccus~~ aureus ~~sigmaA~~ domain 4 ~~complex~~===	+	<StructureSection load='4g8x' size='340' side='right' caption='[[4g8x]], [[Resolution\|resolution]] 3.00Å' scene=''>
-	~~{{ABSTRACT_PUBMED_23178120}}~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[4g8x]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_nctc_8325 Staphylococcus aureus subsp. aureus nctc 8325] and [http://en.wikipedia.org/wiki/Staphylococcus_phage_g1 Staphylococcus phage g1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G8X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4G8X FirstGlance]. <br>
-	==Function==	+	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4g6d\|4g6d]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">plaC, rpoD, SAOUHSC_01662, sigA, Sigma A domain 4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=93061 Staphylococcus aureus subsp. aureus NCTC 8325]), ORF67 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=292029 Staphylococcus phage G1])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4g8x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4g8x OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4g8x RCSB], [http://www.ebi.ac.uk/pdbsum/4g8x PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
	[[http://www.uniprot.org/uniprot/SIGA_STAA8 SIGA_STAA8]] Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is the primary sigma factor during exponential growth.		[[http://www.uniprot.org/uniprot/SIGA_STAA8 SIGA_STAA8]] Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is the primary sigma factor during exponential growth.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Phage G1 gp67 is a 23 kDa protein that binds to the Staphylococcus aureus (Sau) RNA polymerase (RNAP) sigma(A) subunit and blocks cell growth by inhibiting transcription. We show that gp67 has little to no effect on transcription from most promoters but is a potent inhibitor of ribosomal RNA transcription. A 2.0-A-resolution crystal structure of the complex between gp67 and Sau sigma(A) domain 4 (sigma(A)(4)) explains how gp67 joins the RNAP promoter complex through sigma(A)(4) without significantly affecting sigma(A)(4) function. Our results indicate that gp67 forms a complex with RNAP at most, if not all, sigma(A)-dependent promoters, but selectively inhibits promoters that depend on an interaction between upstream DNA and the RNAP alpha-subunit C-terminal domain (alphaCTD). Thus, we reveal a promoter-specific transcription inhibition mechanism by which gp67 interacts with the RNAP promoter complex through one subunit (sigma(A)), and selectively affects the function of another subunit (alphaCTD) depending on promoter usage.
		+
		+	Promoter-specific transcription inhibition in Staphylococcus aureus by a phage protein.,Osmundson J, Montero-Diez C, Westblade LF, Hochschild A, Darst SA Cell. 2012 Nov 21;151(5):1005-16. doi: 10.1016/j.cell.2012.10.034. PMID:23178120<ref>PMID:23178120</ref>

-	~~==About this Structure==~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[4g8x]] is~~ a ~~4 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Staphylococcus_aureus_subsp~~.~~_aureus_nctc_8325 Staphylococcus aureus subsp. aureus nctc 8325] and [http:~~/~~/en.wikipedia.org/wiki/Staphylococcus_phage_g1 Staphylococcus phage g1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G8X OCA].~~	+	</div>

-	==~~Reference~~==	+	==See Also==
-	~~<ref group~~=~~"xtra">PMID:023178120</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	*[[Sigma factor\|Sigma factor]]
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Staphylococcus aureus subsp. aureus nctc 8325]]		[[Category: Staphylococcus aureus subsp. aureus nctc 8325]]
	[[Category: Staphylococcus phage g1]]		[[Category: Staphylococcus phage g1]]
-	[[Category: Darst, S A.]]	+	[[Category: Darst, S A]]
-	[[Category: Hochschild, A.]]	+	[[Category: Hochschild, A]]
-	[[Category: Montero-Diaz, C M.]]	+	[[Category: Montero-Diaz, C M]]
-	[[Category: Osmundson, J S.]]	+	[[Category: Osmundson, J S]]
	[[Category: Dna binding protein]]		[[Category: Dna binding protein]]
	[[Category: Rnap binding protein]]		[[Category: Rnap binding protein]]

OCA at 20:52, 7 August 2013

OCA — Wed, 07 Aug 2013 20:52:40 GMT

←Older revision		Revision as of 20:52, 7 August 2013
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{STRUCTURE_4g8x\| PDB=4g8x \| SCENE= }}
		+	===G1 ORF67 / Staphyloccus aureus sigmaA domain 4 complex===
		+	{{ABSTRACT_PUBMED_23178120}}

-	~~The entry 4g8x~~ is ~~ON HOLD until Paper Publication~~	+	==Function==
		+	[[http://www.uniprot.org/uniprot/SIGA_STAA8 SIGA_STAA8]] Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is the primary sigma factor during exponential growth.

-	~~Authors~~: ~~Darst, S~~.A.~~, Osmundson, J~~.S.~~, Montero-Diaz, C~~.M.~~, Hochschild, A~~.	+	==About this Structure==
		+	[[4g8x]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_nctc_8325 Staphylococcus aureus subsp. aureus nctc 8325] and [http://en.wikipedia.org/wiki/Staphylococcus_phage_g1 Staphylococcus phage g1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G8X OCA].

-	~~Description~~: ~~G1 ORF67~~ / ~~Staphyloccus~~ aureus ~~sigmaA domain 4 complex~~	+	==Reference==
		+	<ref group="xtra">PMID:023178120</ref><references group="xtra"/><references/>
		+	[[Category: Staphylococcus aureus subsp. aureus nctc 8325]]
		+	[[Category: Staphylococcus phage g1]]
		+	[[Category: Darst, S A.]]
		+	[[Category: Hochschild, A.]]
		+	[[Category: Montero-Diaz, C M.]]
		+	[[Category: Osmundson, J S.]]
		+	[[Category: Dna binding protein]]
		+	[[Category: Rnap binding protein]]

OCA at 11:02, 23 January 2013

OCA — Wed, 23 Jan 2013 11:02:41 GMT

←Older revision		Revision as of 11:02, 23 January 2013
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4g8x is ON HOLD	+	The entry 4g8x is ON HOLD until Paper Publication

	Authors: Darst, S.A., Osmundson, J.S., Montero-Diaz, C.M., Hochschild, A.		Authors: Darst, S.A., Osmundson, J.S., Montero-Diaz, C.M., Hochschild, A.

	Description: G1 ORF67 / Staphyloccus aureus sigmaA domain 4 complex		Description: G1 ORF67 / Staphyloccus aureus sigmaA domain 4 complex

OCA: Protected "4g8x" [edit=sysop:move=sysop]

OCA — Wed, 01 Aug 2012 05:31:14 GMT

Protected "4g8x" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:31, 1 August 2012

OCA: New page: '''Unreleased structure''' The entry 4g8x is ON HOLD Authors: Darst, S.A., Osmundson, J.S., Montero-Diaz, C.M., Hochschild, A. Description: G1 ORF67 / Staphyloccus aureus sigmaA domain...

OCA — Wed, 01 Aug 2012 05:31:13 GMT

New page: '''Unreleased structure''' The entry 4g8x is ON HOLD Authors: Darst, S.A., Osmundson, J.S., Montero-Diaz, C.M., Hochschild, A. Description: G1 ORF67 / Staphyloccus aureus sigmaA domain...

New page

'''Unreleased structure'''

The entry 4g8x is ON HOLD

Authors: Darst, S.A., Osmundson, J.S., Montero-Diaz, C.M., Hochschild, A.

Description: G1 ORF67 / Staphyloccus aureus sigmaA domain 4 complex