4gaj - Revision history

OCA at 06:45, 26 October 2022

OCA — Wed, 26 Oct 2022 06:45:33 GMT

←Older revision		Revision as of 06:45, 26 October 2022
Line 3:		Line 3:
	<StructureSection load='4gaj' size='340' side='right'caption='[[4gaj]], [[Resolution\|resolution]] 2.51Å' scene=''>		<StructureSection load='4gaj' size='340' side='right'caption='[[4gaj]], [[Resolution\|resolution]] 2.51Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4gaj]] is a 3 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GAJ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4GAJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4gaj]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Hepacivirus_C Hepacivirus C] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GAJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4GAJ FirstGlance]. <br>
-	</td></tr>~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4gag\|4gag]], [[4gay\|4gay]]</td></tr>~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4gaj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gaj OCA], [https://pdbe.org/4gaj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4gaj RCSB], [https://www.ebi.ac.uk/pdbsum/4gaj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4gaj ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4gaj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gaj OCA], [~~http~~://pdbe.org/4gaj PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4gaj RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4gaj PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4gaj ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q9IY15_9HEPC Q9IY15_9HEPC]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 19:		Line 20:
	==See Also==		==See Also==
	*[[Antibody 3D structures\|Antibody 3D structures]]		*[[Antibody 3D structures\|Antibody 3D structures]]
		+	*[[3D structures of non-human antibody\|3D structures of non-human antibody]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Hepacivirus C]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
-	[[Category: Owsianka, A]]	+	[[Category: Owsianka A]]
-	[[Category: Patel~~, A H~~]]	+	[[Category: Patel AH]]
-	[[Category: Potter~~, J A~~]]	+	[[Category: Potter JA]]
-	[[Category: Taylor~~, G L]]~~	+	[[Category: Taylor GL]]
-	~~[[Category: Antibody fab]]~~	+
-	~~[[Category: Hcv e2 binding]]~~	+
-	~~[[Category: Immune system]]~~	+
-	~~[[Category: Neutralizing antibody~~]]	+

OCA at 09:27, 5 February 2020

OCA — Wed, 05 Feb 2020 09:27:02 GMT

←Older revision		Revision as of 09:27, 5 February 2020
Line 1:		Line 1:

	==Structure of the broadly neutralizing antibody AP33 in complex with its HCV epitope (E2 residues 411-424)==		==Structure of the broadly neutralizing antibody AP33 in complex with its HCV epitope (E2 residues 411-424)==
-	<StructureSection load='4gaj' size='340' side='right' caption='[[4gaj]], [[Resolution\|resolution]] 2.51Å' scene=''>	+	<StructureSection load='4gaj' size='340' side='right'caption='[[4gaj]], [[Resolution\|resolution]] 2.51Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4gaj]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GAJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GAJ FirstGlance]. <br>		<table><tr><td colspan='2'>[[4gaj]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GAJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GAJ FirstGlance]. <br>
Line 16:		Line 16:
	</div>		</div>
	<div class="pdbe-citations 4gaj" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4gaj" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Antibody 3D structures\|Antibody 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
	[[Category: Owsianka, A]]		[[Category: Owsianka, A]]

OCA at 19:56, 24 January 2018

OCA — Wed, 24 Jan 2018 19:56:43 GMT

←Older revision		Revision as of 19:56, 24 January 2018
Line 16:		Line 16:
	</div>		</div>
	<div class="pdbe-citations 4gaj" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4gaj" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[3D structures of antibody\|3D structures of antibody]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 19:56, 15 December 2016

OCA — Thu, 15 Dec 2016 19:56:30 GMT

←Older revision		Revision as of 19:56, 15 December 2016
Line 1:		Line 1:
		+
	==Structure of the broadly neutralizing antibody AP33 in complex with its HCV epitope (E2 residues 411-424)==		==Structure of the broadly neutralizing antibody AP33 in complex with its HCV epitope (E2 residues 411-424)==
	<StructureSection load='4gaj' size='340' side='right' caption='[[4gaj]], [[Resolution\|resolution]] 2.51Å' scene=''>		<StructureSection load='4gaj' size='340' side='right' caption='[[4gaj]], [[Resolution\|resolution]] 2.51Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[4gaj]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GAJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GAJ FirstGlance]. <br>		<table><tr><td colspan='2'>[[4gaj]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GAJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GAJ FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4gag\|4gag]], [[4gay\|4gay]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4gag\|4gag]], [[4gay\|4gay]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4gaj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gaj OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4gaj RCSB], [http://www.ebi.ac.uk/pdbsum/4gaj PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4gaj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gaj OCA], [http://pdbe.org/4gaj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4gaj RCSB], [http://www.ebi.ac.uk/pdbsum/4gaj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4gaj ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 14:		Line 15:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4gaj" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
-	*[[~~Antibody~~\|~~Antibody~~]]	+	*[[3D structures of antibody\|3D structures of antibody]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 09:25, 10 December 2014

OCA — Wed, 10 Dec 2014 09:25:01 GMT

←Older revision		Revision as of 09:25, 10 December 2014
Line 1:		Line 1:
-	[[~~Image:~~4gaj.~~png~~\|~~left~~\|~~200px~~]]	+	==Structure of the broadly neutralizing antibody AP33 in complex with its HCV epitope (E2 residues 411-424)==
		+	<StructureSection load='4gaj' size='340' side='right' caption='[[4gaj]], [[Resolution\|resolution]] 2.51Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4gaj]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GAJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GAJ FirstGlance]. <br>
		+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4gag\|4gag]], [[4gay\|4gay]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4gaj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gaj OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4gaj RCSB], [http://www.ebi.ac.uk/pdbsum/4gaj PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The E2 envelope glycoprotein of HCV binds to the host entry factor CD81, and is the principal target for neutralizing antibodies (nAbs). Most nAbs recognize hypervariable region 1 on E2, which undergoes frequent mutation, thereby allowing the virus to evade neutralization. Consequently, there is great interest in nAbs that target conserved epitopes. One such nAb is AP33, a mouse monoclonal antibody that recognizes a conserved, linear epitope on E2 and potently neutralizes a broad range of HCV genotypes. In this study, the X-ray structure of AP33 Fab in complex with an epitope peptide spanning residues 412 to 423 of HCV E2 has been determined to 1.8A. In the complex, the peptide adopts a beta-hairpin conformation and docks into a deep binding pocket on the antibody. The major determinants of antibody recognition are E2 residues L413, N415, G418 and W420. The structure is compared to the recently described HCV1 Fab in complex with the same epitope. Interestingly, the antigen-binding sites of HCV1 and AP33 are completely different, whereas the peptide conformation is very similar in the two structures. Mutagenesis of the peptide-binding residues on AP33 confirmed that these residues are also critical for AP33 recognition of whole E2, confirming that the peptide-bound structure truly represents AP33 interaction with the intact glycoprotein. The slightly conformation-sensitive character of the AP33-E2 interaction was explored by cross-competition analysis and alanine-scanning mutagenesis. The structural details of this neutralizing epitope provide a starting point for the design of an immunogen capable of eliciting AP33-like antibodies.

-	~~{{STRUCTURE_4gaj\| PDB=4gaj \| SCENE= }}~~	+	Towards a Hepatitis C Vaccine: the Structural Basis of Hepatitis C Virus Neutralization by AP33, a Broadly Neutralizing Antibody.,Potter JA, Owsianka AM, Jeffery N, Matthews DJ, Keck ZY, Lau P, Foung SK, Taylor GL, Patel AH J Virol. 2012 Sep 19. PMID:22993159<ref>PMID:22993159</ref>

-	~~===Structure~~ of the ~~broadly neutralizing antibody AP33 in complex with its HCV epitope (E2 residues 411-424)===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

-	~~{{ABSTRACT_PUBMED_22993159}}~~	+	==See Also==
-		+	*[[Antibody\|Antibody]]
-	==~~About this Structure~~==	+	== References ==
-	[[~~4gaj~~]] ~~is a 3 chain structure with sequence from [http:~~//~~en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GAJ OCA].~~	+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
-	[[Category: Owsianka, A.]]	+	[[Category: Owsianka, A]]
-	[[Category: Patel, A H.]]	+	[[Category: Patel, A H]]
-	[[Category: Potter, J A.]]	+	[[Category: Potter, J A]]
	[[Category: Taylor, G L]]		[[Category: Taylor, G L]]
	[[Category: Antibody fab]]		[[Category: Antibody fab]]

OCA at 09:56, 21 November 2012

OCA — Wed, 21 Nov 2012 09:56:59 GMT

←Older revision		Revision as of 09:56, 21 November 2012
Line 1:		Line 1:
-	[[Image:4gaj.~~jpg~~\|left\|200px]]	+	[[Image:4gaj.png\|left\|200px]]

	{{STRUCTURE_4gaj\| PDB=4gaj \| SCENE= }}		{{STRUCTURE_4gaj\| PDB=4gaj \| SCENE= }}

OCA at 07:20, 10 October 2012

OCA — Wed, 10 Oct 2012 07:20:24 GMT

←Older revision		Revision as of 07:20, 10 October 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:4gaj.jpg\|left\|200px]]

-	~~The entry~~ 4gaj ~~is ON HOLD~~ ~~until Paper Publication~~	+	{{STRUCTURE_4gaj\| PDB=4gaj \| SCENE= }}

-	~~Authors: Potter, J.A., Owsianka, A., Taylor, G.L, Patel, A.H.~~	+	===Structure of the broadly neutralizing antibody AP33 in complex with its HCV epitope (E2 residues 411-424)===

-	~~Description:~~ Structure ~~of the broadly neutralizing antibody AP33 in complex~~ with ~~its HCV epitope (E2 residues 411~~-~~424)~~	+	{{ABSTRACT_PUBMED_22993159}}
		+
		+	==About this Structure==
		+	[[4gaj]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GAJ OCA].
		+	[[Category: Mus musculus]]
		+	[[Category: Owsianka, A.]]
		+	[[Category: Patel, A H.]]
		+	[[Category: Potter, J A.]]
		+	[[Category: Taylor, G L]]
		+	[[Category: Antibody fab]]
		+	[[Category: Hcv e2 binding]]
		+	[[Category: Immune system]]
		+	[[Category: Neutralizing antibody]]

OCA at 04:51, 5 September 2012

OCA — Wed, 05 Sep 2012 04:51:54 GMT

←Older revision		Revision as of 04:51, 5 September 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4gaj is ON HOLD	+	The entry 4gaj is ON HOLD until Paper Publication

	Authors: Potter, J.A., Owsianka, A., Taylor, G.L, Patel, A.H.		Authors: Potter, J.A., Owsianka, A., Taylor, G.L, Patel, A.H.

	Description: Structure of the broadly neutralizing antibody AP33 in complex with its HCV epitope (E2 residues 411-424)		Description: Structure of the broadly neutralizing antibody AP33 in complex with its HCV epitope (E2 residues 411-424)

OCA: Protected "4gaj" [edit=sysop:move=sysop]

OCA — Wed, 01 Aug 2012 05:32:51 GMT

Protected "4gaj" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:32, 1 August 2012

OCA: New page: '''Unreleased structure''' The entry 4gaj is ON HOLD Authors: Potter, J.A., Owsianka, A., Taylor, G.L, Patel, A.H. Description: Structure of the broadly neutralizing antibody AP33 in c...

OCA — Wed, 01 Aug 2012 05:32:50 GMT

New page: '''Unreleased structure''' The entry 4gaj is ON HOLD Authors: Potter, J.A., Owsianka, A., Taylor, G.L, Patel, A.H. Description: Structure of the broadly neutralizing antibody AP33 in c...

New page

'''Unreleased structure'''

The entry 4gaj is ON HOLD

Authors: Potter, J.A., Owsianka, A., Taylor, G.L, Patel, A.H.

Description: Structure of the broadly neutralizing antibody AP33 in complex with its HCV epitope (E2 residues 411-424)