4geq - Revision history

OCA at 15:44, 14 March 2024

OCA — Thu, 14 Mar 2024 15:44:53 GMT

←Older revision		Revision as of 15:44, 14 March 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4geq]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GEQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4GEQ FirstGlance]. <br>		<table><tr><td colspan='2'>[[4geq]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GEQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4GEQ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.01Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4geq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4geq OCA], [https://pdbe.org/4geq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4geq RCSB], [https://www.ebi.ac.uk/pdbsum/4geq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4geq ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4geq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4geq OCA], [https://pdbe.org/4geq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4geq RCSB], [https://www.ebi.ac.uk/pdbsum/4geq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4geq ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/SPC25_YEAST SPC25_YEAST] Acts as a component of the essential kinetochore-associated NDC80 complex, which is involved in chromosome segregation and spindle checkpoint activity.<ref>PMID:11266451</ref> <ref>PMID:12514103</ref>		[https://www.uniprot.org/uniprot/SPC25_YEAST SPC25_YEAST] Acts as a component of the essential kinetochore-associated NDC80 complex, which is involved in chromosome segregation and spindle checkpoint activity.<ref>PMID:11266451</ref> <ref>PMID:12514103</ref>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The Ndc80 complex is the key microtubule-binding element of the kinetochore. In contrast to the well-characterized interaction of Ndc80-Nuf2 heads with microtubules, little is known about how the Spc24-25 heterodimer connects to centromeric chromatin. Here, we present molecular details of Spc24-25 in complex with the histone-fold protein Cnn1/CENP-T illustrating how this connection ultimately links microtubules to chromosomes. The conserved Ndc80 receptor motif of Cnn1 is bound as an alpha helix in a hydrophobic cleft at the interface between Spc24 and Spc25. Point mutations that disrupt the Ndc80-Cnn1 interaction also abrogate binding to the Mtw1 complex and are lethal in yeast. We identify a Cnn1-related motif in the Dsn1 subunit of the Mtw1 complex, necessary for Ndc80 binding and essential for yeast growth. Replacing this region with the Cnn1 peptide restores viability demonstrating functionality of the Ndc80-binding module in different molecular contexts. Finally, phosphorylation of the Cnn1 N-terminus coordinates the binding of the two competing Ndc80 interaction partners. Together, our data provide structural insights into the modular binding mechanism of the Ndc80 complex to its centromere recruiters.
-
-	A structural basis for kinetochore recruitment of the Ndc80 complex via two distinct centromere receptors.,Malvezzi F, Litos G, Schleiffer A, Heuck A, Mechtler K, Clausen T, Westermann S EMBO J. 2013 Feb 6;32(3):409-23. doi: 10.1038/emboj.2012.356. Epub 2013 Jan 18. PMID:23334295<ref>PMID:23334295</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 4geq" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 06:53, 26 October 2022

OCA — Wed, 26 Oct 2022 06:53:02 GMT

←Older revision		Revision as of 06:53, 26 October 2022
Line 1:		Line 1:

	==Crystal structure of the Spc24-Spc25/Cnn1 binding interface==		==Crystal structure of the Spc24-Spc25/Cnn1 binding interface==
-	<StructureSection load='4geq' size='340' side='right' caption='[[4geq]], [[Resolution\|resolution]] 2.01Å' scene=''>	+	<StructureSection load='4geq' size='340' side='right'caption='[[4geq]], [[Resolution\|resolution]] 2.01Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4geq]] is a 6 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Baker's_yeast Baker's yeast~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GEQ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4GEQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4geq]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GEQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4GEQ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SPC25, YER018C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast]), SPC24, YM9718.16C, YMR117C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4geq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4geq OCA], [https://pdbe.org/4geq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4geq RCSB], [https://www.ebi.ac.uk/pdbsum/4geq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4geq ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4geq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4geq OCA], [~~http~~://pdbe.org/4geq PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4geq RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4geq PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4geq ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/SPC25_YEAST SPC25_YEAST]] Acts as a component of the essential kinetochore-associated NDC80 complex, which is involved in chromosome segregation and spindle checkpoint activity.<ref>PMID:11266451</ref> <ref>PMID:12514103</ref> [[http://www.uniprot.org/uniprot/SPC24_YEAST SPC24_YEAST]] Acts as a component of the essential kinetochore-associated NDC80 complex, which is involved in chromosome segregation and spindle checkpoint activity.<ref>PMID:11266451</ref> <ref>PMID:11952896</ref> <ref>PMID:12514103</ref>	+	[https://www.uniprot.org/uniprot/SPC25_YEAST SPC25_YEAST] Acts as a component of the essential kinetochore-associated NDC80 complex, which is involved in chromosome segregation and spindle checkpoint activity.<ref>PMID:11266451</ref> <ref>PMID:12514103</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Baker's yeast~~]]	+	[[Category: Large Structures]]
-	[[Category: Clausen, T]]	+	[[Category: Saccharomyces cerevisiae S288C]]
-	[[Category: Heuck, A]]	+	[[Category: Clausen T]]
-	[[Category: Litos, G]]	+	[[Category: Heuck A]]
-	[[Category: Malvezzi, F]]	+	[[Category: Litos G]]
-	[[Category: Schleiffer, A]]	+	[[Category: Malvezzi F]]
-	[[Category: Westermann, S]]	+	[[Category: Schleiffer A]]
-	~~[[Category: Cell cycle]]~~	+	[[Category: Westermann S]]
-	~~[[Category: Kinetochore component]]~~	+
-	~~[[Category: Ndc80-binding motif]]~~	+
-	~~[[Category: Nucleus]]~~	+
-	~~[[Category: Protein-protein complex]]~~	+
-	~~[[Category: Rwd domain~~]]	+

OCA at 12:07, 12 April 2017

OCA — Wed, 12 Apr 2017 12:07:39 GMT

←Older revision		Revision as of 12:07, 12 April 2017
Line 1:		Line 1:
		+
	==Crystal structure of the Spc24-Spc25/Cnn1 binding interface==		==Crystal structure of the Spc24-Spc25/Cnn1 binding interface==
	<StructureSection load='4geq' size='340' side='right' caption='[[4geq]], [[Resolution\|resolution]] 2.01Å' scene=''>		<StructureSection load='4geq' size='340' side='right' caption='[[4geq]], [[Resolution\|resolution]] 2.01Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SPC25, YER018C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast]), SPC24, YM9718.16C, YMR117C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SPC25, YER018C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast]), SPC24, YM9718.16C, YMR117C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4geq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4geq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4geq RCSB], [http://www.ebi.ac.uk/pdbsum/4geq PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4geq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4geq OCA], [http://pdbe.org/4geq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4geq RCSB], [http://www.ebi.ac.uk/pdbsum/4geq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4geq ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4geq" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 12:34, 1 July 2015

OCA — Wed, 01 Jul 2015 12:34:18 GMT

←Older revision		Revision as of 12:34, 1 July 2015
Line 2:		Line 2:
	<StructureSection load='4geq' size='340' side='right' caption='[[4geq]], [[Resolution\|resolution]] 2.01Å' scene=''>		<StructureSection load='4geq' size='340' side='right' caption='[[4geq]], [[Resolution\|resolution]] 2.01Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4geq]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Saccharomyces_cerevisiae_s288c Saccharomyces cerevisiae s288c~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GEQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GEQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4geq]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GEQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GEQ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SPC25, YER018C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 ~~Saccharomyces cerevisiae S288c~~]), SPC24, YM9718.16C, YMR117C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 ~~Saccharomyces cerevisiae S288c~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SPC25, YER018C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast]), SPC24, YM9718.16C, YMR117C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4geq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4geq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4geq RCSB], [http://www.ebi.ac.uk/pdbsum/4geq PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4geq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4geq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4geq RCSB], [http://www.ebi.ac.uk/pdbsum/4geq PDBsum]</span></td></tr>
	</table>		</table>
Line 21:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Saccharomyces cerevisiae s288c~~]]	+	[[Category: Baker's yeast]]
	[[Category: Clausen, T]]		[[Category: Clausen, T]]
	[[Category: Heuck, A]]		[[Category: Heuck, A]]

OCA at 22:28, 25 December 2014

OCA — Thu, 25 Dec 2014 22:28:56 GMT

←Older revision		Revision as of 22:28, 25 December 2014
Line 7:		Line 7:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4geq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4geq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4geq RCSB], [http://www.ebi.ac.uk/pdbsum/4geq PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4geq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4geq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4geq RCSB], [http://www.ebi.ac.uk/pdbsum/4geq PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/SPC25_YEAST SPC25_YEAST]] Acts as a component of the essential kinetochore-associated NDC80 complex, which is involved in chromosome segregation and spindle checkpoint activity.<ref>PMID:11266451</ref> <ref>PMID:12514103</ref> [[http://www.uniprot.org/uniprot/SPC24_YEAST SPC24_YEAST]] Acts as a component of the essential kinetochore-associated NDC80 complex, which is involved in chromosome segregation and spindle checkpoint activity.<ref>PMID:11266451</ref> <ref>PMID:11952896</ref> <ref>PMID:12514103</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 09:27, 10 December 2014

OCA — Wed, 10 Dec 2014 09:27:34 GMT

←Older revision		Revision as of 09:27, 10 December 2014
Line 1:		Line 1:
-	[[~~Image:~~4geq.~~png~~\|~~left~~\|~~200px~~]]	+	==Crystal structure of the Spc24-Spc25/Cnn1 binding interface==
		+	<StructureSection load='4geq' size='340' side='right' caption='[[4geq]], [[Resolution\|resolution]] 2.01Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4geq]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_s288c Saccharomyces cerevisiae s288c]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GEQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GEQ FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SPC25, YER018C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Saccharomyces cerevisiae S288c]), SPC24, YM9718.16C, YMR117C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Saccharomyces cerevisiae S288c])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4geq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4geq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4geq RCSB], [http://www.ebi.ac.uk/pdbsum/4geq PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The Ndc80 complex is the key microtubule-binding element of the kinetochore. In contrast to the well-characterized interaction of Ndc80-Nuf2 heads with microtubules, little is known about how the Spc24-25 heterodimer connects to centromeric chromatin. Here, we present molecular details of Spc24-25 in complex with the histone-fold protein Cnn1/CENP-T illustrating how this connection ultimately links microtubules to chromosomes. The conserved Ndc80 receptor motif of Cnn1 is bound as an alpha helix in a hydrophobic cleft at the interface between Spc24 and Spc25. Point mutations that disrupt the Ndc80-Cnn1 interaction also abrogate binding to the Mtw1 complex and are lethal in yeast. We identify a Cnn1-related motif in the Dsn1 subunit of the Mtw1 complex, necessary for Ndc80 binding and essential for yeast growth. Replacing this region with the Cnn1 peptide restores viability demonstrating functionality of the Ndc80-binding module in different molecular contexts. Finally, phosphorylation of the Cnn1 N-terminus coordinates the binding of the two competing Ndc80 interaction partners. Together, our data provide structural insights into the modular binding mechanism of the Ndc80 complex to its centromere recruiters.

-	~~{{STRUCTURE_4geq\| PDB=4geq \| SCENE= }}~~	+	A structural basis for kinetochore recruitment of the Ndc80 complex via two distinct centromere receptors.,Malvezzi F, Litos G, Schleiffer A, Heuck A, Mechtler K, Clausen T, Westermann S EMBO J. 2013 Feb 6;32(3):409-23. doi: 10.1038/emboj.2012.356. Epub 2013 Jan 18. PMID:23334295<ref>PMID:23334295</ref>

-	~~===Crystal structure~~ of the ~~Spc24-Spc25~~/~~Cnn1 binding interface===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_23334295}}~~	+	== References ==
-		+	<references/>
-	==~~About this Structure~~==	+	__TOC__
-	~~[[4geq]] is a 6 chain structure with sequence from [http:~~//~~en.wikipedia.org/wiki/Saccharomyces_cerevisiae_s288c Saccharomyces cerevisiae s288c]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GEQ OCA].~~	+	</StructureSection>
	[[Category: Saccharomyces cerevisiae s288c]]		[[Category: Saccharomyces cerevisiae s288c]]
-	[[Category: Clausen, T.]]	+	[[Category: Clausen, T]]
-	[[Category: Heuck, A.]]	+	[[Category: Heuck, A]]
-	[[Category: Litos, G.]]	+	[[Category: Litos, G]]
-	[[Category: Malvezzi, F.]]	+	[[Category: Malvezzi, F]]
-	[[Category: Schleiffer, A.]]	+	[[Category: Schleiffer, A]]
-	[[Category: Westermann, S.]]	+	[[Category: Westermann, S]]
	[[Category: Cell cycle]]		[[Category: Cell cycle]]
	[[Category: Kinetochore component]]		[[Category: Kinetochore component]]

OCA at 13:02, 20 February 2013

OCA — Wed, 20 Feb 2013 13:02:01 GMT

←Older revision		Revision as of 13:02, 20 February 2013
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-	[[Image:4geq.~~jpg~~\|left\|200px]]	+	[[Image:4geq.png\|left\|200px]]

	{{STRUCTURE_4geq\| PDB=4geq \| SCENE= }}		{{STRUCTURE_4geq\| PDB=4geq \| SCENE= }}
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	===Crystal structure of the Spc24-Spc25/Cnn1 binding interface===		===Crystal structure of the Spc24-Spc25/Cnn1 binding interface===

		+	{{ABSTRACT_PUBMED_23334295}}

	==About this Structure==		==About this Structure==

OCA at 12:39, 30 January 2013

OCA — Wed, 30 Jan 2013 12:39:54 GMT

←Older revision		Revision as of 12:39, 30 January 2013
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-	~~'''Unreleased structure'''~~	+	[[Image:4geq.jpg\|left\|200px]]

-	~~The entry~~ 4geq ~~is ON HOLD~~ ~~until Paper Publication~~	+	{{STRUCTURE_4geq\| PDB=4geq \| SCENE= }}

-	~~Authors: Malvezzi, F., Litos, G., Schleiffer, A., Heuck, A., Clausen, T., Westermann, S.~~	+	===Crystal structure of the Spc24-Spc25/Cnn1 binding interface===

-	~~Description: Crystal~~ structure ~~of the Spc24~~-~~Spc25~~/~~Cnn1~~ binding ~~interface~~	+
		+	==About this Structure==
		+	[[4geq]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_s288c Saccharomyces cerevisiae s288c]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GEQ OCA].
		+	[[Category: Saccharomyces cerevisiae s288c]]
		+	[[Category: Clausen, T.]]
		+	[[Category: Heuck, A.]]
		+	[[Category: Litos, G.]]
		+	[[Category: Malvezzi, F.]]
		+	[[Category: Schleiffer, A.]]
		+	[[Category: Westermann, S.]]
		+	[[Category: Cell cycle]]
		+	[[Category: Kinetochore component]]
		+	[[Category: Ndc80-binding motif]]
		+	[[Category: Nucleus]]
		+	[[Category: Protein-protein complex]]
		+	[[Category: Rwd domain]]

OCA at 10:56, 19 December 2012

OCA — Wed, 19 Dec 2012 10:56:16 GMT

←Older revision		Revision as of 10:56, 19 December 2012
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4geq is ON HOLD	+	The entry 4geq is ON HOLD until Paper Publication

	Authors: Malvezzi, F., Litos, G., Schleiffer, A., Heuck, A., Clausen, T., Westermann, S.		Authors: Malvezzi, F., Litos, G., Schleiffer, A., Heuck, A., Clausen, T., Westermann, S.

	Description: Crystal structure of the Spc24-Spc25/Cnn1 binding interface		Description: Crystal structure of the Spc24-Spc25/Cnn1 binding interface

OCA: Protected "4geq" [edit=sysop:move=sysop]

OCA — Wed, 08 Aug 2012 05:02:37 GMT

Protected "4geq" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:02, 8 August 2012