4gga - Revision history

OCA at 06:55, 26 October 2022

OCA — Wed, 26 Oct 2022 06:55:12 GMT

←Older revision		Revision as of 06:55, 26 October 2022
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	==Structural Analysis of Human Cdc20 Supports Multi-site Degron Recognition by APC/C==		==Structural Analysis of Human Cdc20 Supports Multi-site Degron Recognition by APC/C==
-	<StructureSection load='4gga' size='340' side='right' caption='[[4gga]], [[Resolution\|resolution]] 2.04Å' scene=''>	+	<StructureSection load='4gga' size='340' side='right'caption='[[4gga]], [[Resolution\|resolution]] 2.04Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4gga]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GGA OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4GGA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4gga]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GGA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4GGA FirstGlance]. <br>
-	</td></tr>~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ggc\|4ggc]]</td></tr>~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4gga FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gga OCA], [https://pdbe.org/4gga PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4gga RCSB], [https://www.ebi.ac.uk/pdbsum/4gga PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4gga ProSAT]</span></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CDC20 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>~~	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4gga FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gga OCA], [~~http~~://pdbe.org/4gga PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4gga RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4gga PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4gga ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/CDC20_HUMAN CDC20_HUMAN]] Required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex. Is regulated by MAD2L1: in metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates. The CDC20-APC/C complex positively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. CDC20-APC/C-induced degradation of NEUROD2 induces presynaptic differentiation.<ref>PMID:9811605</ref> <ref>PMID:9734353</ref> <ref>PMID:9637688</ref>	+	[https://www.uniprot.org/uniprot/CDC20_HUMAN CDC20_HUMAN] Required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex. Is regulated by MAD2L1: in metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates. The CDC20-APC/C complex positively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. CDC20-APC/C-induced degradation of NEUROD2 induces presynaptic differentiation.<ref>PMID:9811605</ref> <ref>PMID:9734353</ref> <ref>PMID:9637688</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
-	[[Category: ~~Luo, X~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Tian, W~~]]	+	[[Category: Luo X]]
-	[[Category: ~~Tomchick, D R~~]]	+	[[Category: Tian W]]
-	[[Category: ~~Cell cycle]]~~	+	[[Category: Tomchick DR]]
-	~~[[Category: Mitosis]]~~	+
-	~~[[Category: Securin]]~~	+
-	~~[[Category: Ubiquitination]]~~	+
-	~~[[Category: Wd40~~]]	+

OCA at 12:42, 11 August 2016

OCA — Thu, 11 Aug 2016 12:42:11 GMT

←Older revision		Revision as of 12:42, 11 August 2016
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		+
	==Structural Analysis of Human Cdc20 Supports Multi-site Degron Recognition by APC/C==		==Structural Analysis of Human Cdc20 Supports Multi-site Degron Recognition by APC/C==
	<StructureSection load='4gga' size='340' side='right' caption='[[4gga]], [[Resolution\|resolution]] 2.04Å' scene=''>		<StructureSection load='4gga' size='340' side='right' caption='[[4gga]], [[Resolution\|resolution]] 2.04Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4gga]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GGA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GGA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4gga]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GGA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GGA FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ggc\|4ggc]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ggc\|4ggc]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CDC20 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 ~~Homo sapiens~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CDC20 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4gga FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gga OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4gga RCSB], [http://www.ebi.ac.uk/pdbsum/4gga PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4gga FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gga OCA], [http://pdbe.org/4gga PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4gga RCSB], [http://www.ebi.ac.uk/pdbsum/4gga PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4gga ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/CDC20_HUMAN CDC20_HUMAN]] Required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex. Is regulated by MAD2L1: in metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates. The CDC20-APC/C complex positively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. CDC20-APC/C-induced degradation of NEUROD2 induces presynaptic differentiation.<ref>PMID:9811605</ref> <ref>PMID:9734353</ref> <ref>PMID:9637688</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 15:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4gga" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Homo sapiens~~]]	+	[[Category: Human]]
	[[Category: Luo, X]]		[[Category: Luo, X]]
	[[Category: Tian, W]]		[[Category: Tian, W]]

OCA at 14:31, 5 January 2015

OCA — Mon, 05 Jan 2015 14:31:01 GMT

←Older revision		Revision as of 14:31, 5 January 2015
Line 1:		Line 1:
-	[[~~Image:~~4gga.~~png~~\|~~left~~\|~~200px~~]]	+	==Structural Analysis of Human Cdc20 Supports Multi-site Degron Recognition by APC/C==
		+	<StructureSection load='4gga' size='340' side='right' caption='[[4gga]], [[Resolution\|resolution]] 2.04Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4gga]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GGA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GGA FirstGlance]. <br>
		+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ggc\|4ggc]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CDC20 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4gga FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gga OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4gga RCSB], [http://www.ebi.ac.uk/pdbsum/4gga PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The anaphase-promoting complex/cyclosome (APC/C) promotes anaphase onset and mitotic exit through ubiquitinating securin and cyclin B1. The mitotic APC/C activator, the cell division cycle 20 (Cdc20) protein, directly interacts with APC/C degrons--the destruction (D) and KEN boxes. APC/C(Cdc20) is the target of the spindle checkpoint. Checkpoint inhibition of APC/C(Cdc20) requires the binding of a BubR1 KEN box to Cdc20. How APC/C recognizes substrates is not understood. We report the crystal structures of human Cdc20 alone or bound to a BubR1 KEN box. Cdc20 has a disordered N-terminal region and a C-terminal WD40 beta propeller with a preformed KEN-box-binding site at its top face. We identify a second conserved surface at the side of the Cdc20 beta propeller as a D-box-binding site. The D box of securin, but not its KEN box, is critical for securin ubiquitination by APC/C(Cdc20). Although both motifs contribute to securin ubiquitination by APC/C(Cdh1), securin mutants lacking either motif are efficiently ubiquitinated. Furthermore, D-box peptides diminish the ubiquitination of KEN-box substrates by APC/C(Cdh1), suggesting possible competition between the two motifs. Our results indicate the lack of strong positive cooperativity between the two degrons of securin. We propose that low-cooperativity, multisite target recognition enables APC/C to robustly ubiquitinate diverse substrates and helps to drive cell cycle oscillations.

-	~~{{STRUCTURE_4gga\| PDB=4gga \| SCENE= }}~~	+	Structural analysis of human Cdc20 supports multisite degron recognition by APC/C.,Tian W, Li B, Warrington R, Tomchick DR, Yu H, Luo X Proc Natl Acad Sci U S A. 2012 Nov 6;109(45):18419-24. doi:, 10.1073/pnas.1213438109. Epub 2012 Oct 22. PMID:23091007<ref>PMID:23091007</ref>

-	~~===Structural Analysis~~ of ~~Human Cdc20 Supports Multi-site Degron Recognition by APC~~/~~C===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_23091007}}~~	+	== References ==
-		+	<references/>
-	==~~About this Structure~~==	+	__TOC__
-	~~[[4gga]] is a 1 chain structure with sequence from [http:~~//~~en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GGA OCA].~~	+	</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
-	[[Category: Luo, X.]]	+	[[Category: Luo, X]]
-	[[Category: Tian, W.]]	+	[[Category: Tian, W]]
-	[[Category: Tomchick, D R.]]	+	[[Category: Tomchick, D R]]
	[[Category: Cell cycle]]		[[Category: Cell cycle]]
	[[Category: Mitosis]]		[[Category: Mitosis]]

OCA at 08:10, 2 January 2013

OCA — Wed, 02 Jan 2013 08:10:15 GMT

←Older revision		Revision as of 08:10, 2 January 2013
Line 1:		Line 1:
-	[[Image:4gga.~~jpg~~\|left\|200px]]	+	[[Image:4gga.png\|left\|200px]]

	{{STRUCTURE_4gga\| PDB=4gga \| SCENE= }}		{{STRUCTURE_4gga\| PDB=4gga \| SCENE= }}
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	===Structural Analysis of Human Cdc20 Supports Multi-site Degron Recognition by APC/C===		===Structural Analysis of Human Cdc20 Supports Multi-site Degron Recognition by APC/C===

		+	{{ABSTRACT_PUBMED_23091007}}

	==About this Structure==		==About this Structure==

OCA at 13:47, 8 November 2012

OCA — Thu, 08 Nov 2012 13:47:34 GMT

←Older revision		Revision as of 13:47, 8 November 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:4gga.jpg\|left\|200px]]

-	~~The entry~~ 4gga ~~is ON HOLD~~ ~~until Paper Publication~~	+	{{STRUCTURE_4gga\| PDB=4gga \| SCENE= }}

-	~~Authors: Luo, X., Tian, W., Tomchick, D.R.~~	+	===Structural Analysis of Human Cdc20 Supports Multi-site Degron Recognition by APC/C===

-	~~Description~~: ~~Structural Analysis of Human Cdc20 Supports Multi~~-~~site Degron Recognition by APC~~/C	+
		+	==About this Structure==
		+	[[4gga]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GGA OCA].
		+	[[Category: Homo sapiens]]
		+	[[Category: Luo, X.]]
		+	[[Category: Tian, W.]]
		+	[[Category: Tomchick, D R.]]
		+	[[Category: Cell cycle]]
		+	[[Category: Mitosis]]
		+	[[Category: Securin]]
		+	[[Category: Ubiquitination]]
		+	[[Category: Wd40]]

OCA at 06:52, 10 October 2012

OCA — Wed, 10 Oct 2012 06:52:10 GMT

←Older revision		Revision as of 06:52, 10 October 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4gga is ON HOLD	+	The entry 4gga is ON HOLD until Paper Publication

	Authors: Luo, X., Tian, W., Tomchick, D.R.		Authors: Luo, X., Tian, W., Tomchick, D.R.

-	Description: Structural Analysis of Human Cdc20 Supports ~~Anti-cooperative~~ Multi-site Degron Recognition by APC/C	+	Description: Structural Analysis of Human Cdc20 Supports Multi-site Degron Recognition by APC/C

OCA: Protected "4gga" [edit=sysop:move=sysop]

OCA — Wed, 15 Aug 2012 05:25:32 GMT

Protected "4gga" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:25, 15 August 2012

OCA: New page: '''Unreleased structure''' The entry 4gga is ON HOLD Authors: Luo, X., Tian, W., Tomchick, D.R. Description: Structural Analysis of Human Cdc20 Supports Anti-cooperative Multi-site Deg...

OCA — Wed, 15 Aug 2012 05:25:31 GMT

New page: '''Unreleased structure''' The entry 4gga is ON HOLD Authors: Luo, X., Tian, W., Tomchick, D.R. Description: Structural Analysis of Human Cdc20 Supports Anti-cooperative Multi-site Deg...

New page

'''Unreleased structure'''

The entry 4gga is ON HOLD

Authors: Luo, X., Tian, W., Tomchick, D.R.

Description: Structural Analysis of Human Cdc20 Supports Anti-cooperative Multi-site Degron Recognition by APC/C