4gko - Revision history

OCA at 15:49, 14 March 2024

OCA — Thu, 14 Mar 2024 15:49:20 GMT

←Older revision		Revision as of 15:49, 14 March 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4gko]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GKO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4GKO FirstGlance]. <br>		<table><tr><td colspan='2'>[[4gko]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GKO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4GKO FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.3Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4gko FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gko OCA], [https://pdbe.org/4gko PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4gko RCSB], [https://www.ebi.ac.uk/pdbsum/4gko PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4gko ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4gko FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gko OCA], [https://pdbe.org/4gko PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4gko RCSB], [https://www.ebi.ac.uk/pdbsum/4gko PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4gko ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/IGHE_HUMAN IGHE_HUMAN]		[https://www.uniprot.org/uniprot/IGHE_HUMAN IGHE_HUMAN]
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Immunoglobulin E (IgE) antibodies play a fundamental role in allergic disease and are a target for therapeutic intervention. IgE functions principally through two receptors, FcepsilonRI and CD23 (FcepsilonRII). Minute amounts of allergen trigger mast cell or basophil degranulation by cross-linking IgE-bound FcepsilonRI, leading to an inflammatory response. The interaction between IgE and CD23 on B-cells regulates IgE synthesis. CD23 is unique among Ig receptors in that it belongs to the C-type (calcium-dependent) lectin-like superfamily. Although the interaction of CD23 with IgE is carbohydrate-independent, calcium has been reported to increase the affinity for IgE, but the structural basis for this activity has previously been unknown. We have determined the crystal structures of the human lectin-like head domain of CD23 in its Ca(2+)-free and Ca(2+)-bound forms, as well as the crystal structure of the Ca(2+)-bound head domain of CD23 in complex with a subfragment of IgE-Fc consisting of the dimer of Cepsilon3 and Cepsilon4 domains (Fcepsilon3-4). Together with site-directed mutagenesis, the crystal structures of four Ca(2+) ligand mutants, isothermal titration calorimetry, surface plasmon resonance, and stopped-flow analysis, we demonstrate that Ca(2+) binds at the principal and evolutionarily conserved binding site in CD23. Ca(2+) binding drives Pro-250, at the base of an IgE-binding loop (loop 4), from the trans to the cis configuration with a concomitant conformational change and ordering of residues in the loop. These Ca(2+)-induced structural changes in CD23 lead to additional interactions with IgE, a more entropically favorable interaction, and a 30-fold increase in affinity of a single head domain of CD23 for IgE. Taken together, these results suggest that binding of Ca(2+) brings an extra degree of modulation to CD23 function.
-
-	Ca2+-dependent Structural Changes in the B-cell Receptor CD23 Increase Its Affinity for Human Immunoglobulin E.,Yuan D, Keeble AH, Hibbert RG, Fabiane S, Gould HJ, McDonnell JM, Beavil AJ, Sutton BJ, Dhaliwal B J Biol Chem. 2013 Jul 26;288(30):21667-77. doi: 10.1074/jbc.M113.480657. Epub, 2013 Jun 17. PMID:23775083<ref>PMID:23775083</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 4gko" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[CD23\|CD23]]		*[[CD23\|CD23]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 07:02, 26 October 2022

OCA — Wed, 26 Oct 2022 07:02:22 GMT

←Older revision		Revision as of 07:02, 26 October 2022
Line 1:		Line 1:

	==Crystal structure of the calcium2+-bound human IgE-Fc(epsilon)3-4 bound to its B cell receptor derCD23==		==Crystal structure of the calcium2+-bound human IgE-Fc(epsilon)3-4 bound to its B cell receptor derCD23==
-	<StructureSection load='4gko' size='340' side='right' caption='[[4gko]], [[Resolution\|resolution]] 3.30Å' scene=''>	+	<StructureSection load='4gko' size='340' side='right'caption='[[4gko]], [[Resolution\|resolution]] 3.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4gko]] is a 12 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GKO OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4GKO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4gko]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GKO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4GKO FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1t8c\|1t8c]], [[1t8d\|1t8d]], [[2h2r\|2h2r]], [[2h2t\|2h2t]], [[3ha0\|3ha0]], [[3h9y\|3h9y]], [[4ezm\|4ezm]], [[4g96\|4g96]], [[4g9a\|4g9a]], [[4gi0\|4gi0]], [[4gj0\|4gj0]], [[4gjx\|4gjx]], [[4gk1\|4gk1]]</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4gko FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gko OCA], [https://pdbe.org/4gko PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4gko RCSB], [https://www.ebi.ac.uk/pdbsum/4gko PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4gko ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">IGHE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), FCER2, CD23A, CLEC4J, FCE2, IGEBF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4gko FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gko OCA], [~~http~~://pdbe.org/4gko PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4gko RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4gko PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4gko ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/~~FCER2_HUMAN FCER2_HUMAN~~]~~] Low-affinity receptor for immunoglobulin E (IgE) and CR2/CD21. Has essential roles in the regulation of IgE production and in the differentiation of B-cells (it is a B-cell-specific antigen).~~	+	[https://www.uniprot.org/uniprot/IGHE_HUMAN IGHE_HUMAN]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 20:		Line 18:
	</div>		</div>
	<div class="pdbe-citations 4gko" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4gko" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[CD23\|CD23]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
-	[[Category: ~~Dhaliwal, B~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Sutton,~~ B J]]	+	[[Category: Dhaliwal B]]
-	[[Category: ~~Yuan, D~~]]	+	[[Category: Sutton BJ]]
-	[[Category: ~~Antibody receptor]]~~	+	[[Category: Yuan D]]
-	~~[[Category: Immune system]]~~	+
-	~~[[Category: Immunoglobulin fold lectin~~]]	+

OCA at 04:48, 5 August 2016

OCA — Fri, 05 Aug 2016 04:48:07 GMT

←Older revision		Revision as of 04:48, 5 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of the calcium2+-bound human IgE-Fc(epsilon)3-4 bound to its B cell receptor derCD23==		==Crystal structure of the calcium2+-bound human IgE-Fc(epsilon)3-4 bound to its B cell receptor derCD23==
	<StructureSection load='4gko' size='340' side='right' caption='[[4gko]], [[Resolution\|resolution]] 3.30Å' scene=''>		<StructureSection load='4gko' size='340' side='right' caption='[[4gko]], [[Resolution\|resolution]] 3.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4gko]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Clostridium_acetobutylicum Clostridium acetobutylicum] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GKO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GKO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4gko]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GKO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GKO FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1t8c\|1t8c]], [[1t8d\|1t8d]], [[2h2r\|2h2r]], [[2h2t\|2h2t]], [[3ha0\|3ha0]], [[3h9y\|3h9y]], [[4ezm\|4ezm]], [[4g96\|4g96]], [[4g9a\|4g9a]], [[4gi0\|4gi0]], [[4gj0\|4gj0]], [[4gjx\|4gjx]], [[4gk1\|4gk1]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1t8c\|1t8c]], [[1t8d\|1t8d]], [[2h2r\|2h2r]], [[2h2t\|2h2t]], [[3ha0\|3ha0]], [[3h9y\|3h9y]], [[4ezm\|4ezm]], [[4g96\|4g96]], [[4g9a\|4g9a]], [[4gi0\|4gi0]], [[4gj0\|4gj0]], [[4gjx\|4gjx]], [[4gk1\|4gk1]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">IGHE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 ~~Homo sapiens~~]), FCER2, CD23A, CLEC4J, FCE2, IGEBF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=~~1488 Clostridium acetobutylicum~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">IGHE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), FCER2, CD23A, CLEC4J, FCE2, IGEBF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4gko FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gko OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4gko RCSB], [http://www.ebi.ac.uk/pdbsum/4gko PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4gko FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gko OCA], [http://pdbe.org/4gko PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4gko RCSB], [http://www.ebi.ac.uk/pdbsum/4gko PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4gko ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4gko" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Clostridium acetobutylicum]]~~	+	[[Category: Human]]
-	~~[[Category: Homo sapiens~~]]	+
	[[Category: Dhaliwal, B]]		[[Category: Dhaliwal, B]]
	[[Category: Sutton, B J]]		[[Category: Sutton, B J]]

OCA at 15:54, 25 December 2014

OCA — Thu, 25 Dec 2014 15:54:13 GMT

←Older revision		Revision as of 15:54, 25 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_4gko\| PDB=4gko \| SCENE= }}~~	+	==Crystal structure of the calcium2+-bound human IgE-Fc(epsilon)3-4 bound to its B cell receptor derCD23==
-	===Crystal structure of the calcium2+-bound human IgE-Fc(epsilon)3-4 bound to its B cell receptor derCD23===	+	<StructureSection load='4gko' size='340' side='right' caption='[[4gko]], [[Resolution\|resolution]] 3.30Å' scene=''>
-	~~{{ABSTRACT_PUBMED_23775083}}~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[4gko]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_acetobutylicum Clostridium acetobutylicum] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GKO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GKO FirstGlance]. <br>
-	==Function==	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1t8c\|1t8c]], [[1t8d\|1t8d]], [[2h2r\|2h2r]], [[2h2t\|2h2t]], [[3ha0\|3ha0]], [[3h9y\|3h9y]], [[4ezm\|4ezm]], [[4g96\|4g96]], [[4g9a\|4g9a]], [[4gi0\|4gi0]], [[4gj0\|4gj0]], [[4gjx\|4gjx]], [[4gk1\|4gk1]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">IGHE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), FCER2, CD23A, CLEC4J, FCE2, IGEBF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1488 Clostridium acetobutylicum])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4gko FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gko OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4gko RCSB], [http://www.ebi.ac.uk/pdbsum/4gko PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
	[[http://www.uniprot.org/uniprot/FCER2_HUMAN FCER2_HUMAN]] Low-affinity receptor for immunoglobulin E (IgE) and CR2/CD21. Has essential roles in the regulation of IgE production and in the differentiation of B-cells (it is a B-cell-specific antigen).		[[http://www.uniprot.org/uniprot/FCER2_HUMAN FCER2_HUMAN]] Low-affinity receptor for immunoglobulin E (IgE) and CR2/CD21. Has essential roles in the regulation of IgE production and in the differentiation of B-cells (it is a B-cell-specific antigen).
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Immunoglobulin E (IgE) antibodies play a fundamental role in allergic disease and are a target for therapeutic intervention. IgE functions principally through two receptors, FcepsilonRI and CD23 (FcepsilonRII). Minute amounts of allergen trigger mast cell or basophil degranulation by cross-linking IgE-bound FcepsilonRI, leading to an inflammatory response. The interaction between IgE and CD23 on B-cells regulates IgE synthesis. CD23 is unique among Ig receptors in that it belongs to the C-type (calcium-dependent) lectin-like superfamily. Although the interaction of CD23 with IgE is carbohydrate-independent, calcium has been reported to increase the affinity for IgE, but the structural basis for this activity has previously been unknown. We have determined the crystal structures of the human lectin-like head domain of CD23 in its Ca(2+)-free and Ca(2+)-bound forms, as well as the crystal structure of the Ca(2+)-bound head domain of CD23 in complex with a subfragment of IgE-Fc consisting of the dimer of Cepsilon3 and Cepsilon4 domains (Fcepsilon3-4). Together with site-directed mutagenesis, the crystal structures of four Ca(2+) ligand mutants, isothermal titration calorimetry, surface plasmon resonance, and stopped-flow analysis, we demonstrate that Ca(2+) binds at the principal and evolutionarily conserved binding site in CD23. Ca(2+) binding drives Pro-250, at the base of an IgE-binding loop (loop 4), from the trans to the cis configuration with a concomitant conformational change and ordering of residues in the loop. These Ca(2+)-induced structural changes in CD23 lead to additional interactions with IgE, a more entropically favorable interaction, and a 30-fold increase in affinity of a single head domain of CD23 for IgE. Taken together, these results suggest that binding of Ca(2+) brings an extra degree of modulation to CD23 function.

-	~~==About this Structure==~~	+	Ca2+-dependent Structural Changes in the B-cell Receptor CD23 Increase Its Affinity for Human Immunoglobulin E.,Yuan D, Keeble AH, Hibbert RG, Fabiane S, Gould HJ, McDonnell JM, Beavil AJ, Sutton BJ, Dhaliwal B J Biol Chem. 2013 Jul 26;288(30):21667-77. doi: 10.1074/jbc.M113.480657. Epub, 2013 Jun 17. PMID:23775083<ref>PMID:23775083</ref>
-	~~[[4gko]] is a 12 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Clostridium_acetobutylicum Clostridium acetobutylicum] and [http~~:~~//en~~.~~wikipedia~~.~~org~~/~~wiki/Homo_sapiens Homo sapiens]~~. ~~Full crystallographic information is available from [http://oca~~.~~weizmann~~.ac.il/~~oca-bin/ocashort?id=4GKO OCA].~~	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group="xtra">PMID:023775083</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Clostridium acetobutylicum]]		[[Category: Clostridium acetobutylicum]]
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
-	[[Category: Dhaliwal, B.]]	+	[[Category: Dhaliwal, B]]
-	[[Category: Sutton, B J.]]	+	[[Category: Sutton, B J]]
-	[[Category: Yuan, D.]]	+	[[Category: Yuan, D]]
	[[Category: Antibody receptor]]		[[Category: Antibody receptor]]
	[[Category: Immune system]]		[[Category: Immune system]]
	[[Category: Immunoglobulin fold lectin]]		[[Category: Immunoglobulin fold lectin]]

OCA at 06:39, 14 August 2013

OCA — Wed, 14 Aug 2013 06:39:45 GMT

←Older revision		Revision as of 06:39, 14 August 2013
Line 1:		Line 1:
	{{STRUCTURE_4gko\| PDB=4gko \| SCENE= }}		{{STRUCTURE_4gko\| PDB=4gko \| SCENE= }}
	===Crystal structure of the calcium2+-bound human IgE-Fc(epsilon)3-4 bound to its B cell receptor derCD23===		===Crystal structure of the calcium2+-bound human IgE-Fc(epsilon)3-4 bound to its B cell receptor derCD23===
		+	{{ABSTRACT_PUBMED_23775083}}

	==Function==		==Function==
Line 7:		Line 8:
	==About this Structure==		==About this Structure==
	[[4gko]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_acetobutylicum Clostridium acetobutylicum] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GKO OCA].		[[4gko]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_acetobutylicum Clostridium acetobutylicum] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GKO OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:023775083</ref><references group="xtra"/><references/>
	[[Category: Clostridium acetobutylicum]]		[[Category: Clostridium acetobutylicum]]
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]

OCA at 10:26, 30 June 2013

OCA — Sun, 30 Jun 2013 10:26:02 GMT

←Older revision		Revision as of 10:26, 30 June 2013
Line 1:		Line 1:
-	~~'''Unreleased~~ structure~~'''~~	+	{{STRUCTURE_4gko\| PDB=4gko \| SCENE= }}
		+	===Crystal structure of the calcium2+-bound human IgE-Fc(epsilon)3-4 bound to its B cell receptor derCD23===

-	~~The entry 4gko~~ is ~~ON HOLD until Paper Publication~~	+	==Function==
		+	[[http://www.uniprot.org/uniprot/FCER2_HUMAN FCER2_HUMAN]] Low-affinity receptor for immunoglobulin E (IgE) and CR2/CD21. Has essential roles in the regulation of IgE production and in the differentiation of B-cells (it is a B-cell-specific antigen).

-	~~Authors~~: ~~Yuan, D~~., Sutton, B.J., ~~Dhaliwal, B~~.	+	==About this Structure==
-		+	[[4gko]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_acetobutylicum Clostridium acetobutylicum] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GKO OCA].
-	~~Description~~: ~~Crystal structure of the calcium2+-bound human IgE-Fc(epsilon)3-4 bound to its B cell~~ receptor ~~derCD23~~	+	[[Category: Clostridium acetobutylicum]]
		+	[[Category: Homo sapiens]]
		+	[[Category: Dhaliwal, B.]]
		+	[[Category: Sutton, B J.]]
		+	[[Category: Yuan, D.]]
		+	[[Category: Antibody receptor]]
		+	[[Category: Immune system]]
		+	[[Category: Immunoglobulin fold lectin]]

OCA at 04:54, 5 September 2012

OCA — Wed, 05 Sep 2012 04:54:05 GMT

←Older revision		Revision as of 04:54, 5 September 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4gko is ON HOLD	+	The entry 4gko is ON HOLD until Paper Publication

	Authors: Yuan, D., Sutton, B.J., Dhaliwal, B.		Authors: Yuan, D., Sutton, B.J., Dhaliwal, B.

	Description: Crystal structure of the calcium2+-bound human IgE-Fc(epsilon)3-4 bound to its B cell receptor derCD23		Description: Crystal structure of the calcium2+-bound human IgE-Fc(epsilon)3-4 bound to its B cell receptor derCD23

OCA: Protected "4gko" [edit=sysop:move=sysop]

OCA — Wed, 22 Aug 2012 05:36:02 GMT

Protected "4gko" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:36, 22 August 2012

OCA: New page: '''Unreleased structure''' The entry 4gko is ON HOLD Authors: Yuan, D., Sutton, B.J., Dhaliwal, B. Description: Crystal structure of the calcium2+-bound human IgE-Fc(epsilon)3-4 bound ...

OCA — Wed, 22 Aug 2012 05:36:01 GMT

New page: '''Unreleased structure''' The entry 4gko is ON HOLD Authors: Yuan, D., Sutton, B.J., Dhaliwal, B. Description: Crystal structure of the calcium2+-bound human IgE-Fc(epsilon)3-4 bound ...

New page

'''Unreleased structure'''

The entry 4gko is ON HOLD

Authors: Yuan, D., Sutton, B.J., Dhaliwal, B.

Description: Crystal structure of the calcium2+-bound human IgE-Fc(epsilon)3-4 bound to its B cell receptor derCD23