4gsk - Revision history

OCA at 15:55, 14 March 2024

OCA — Thu, 14 Mar 2024 15:55:20 GMT

←Older revision		Revision as of 15:55, 14 March 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4gsk]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GSK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4GSK FirstGlance]. <br>		<table><tr><td colspan='2'>[[4gsk]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GSK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4GSK FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.9Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4gsk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gsk OCA], [https://pdbe.org/4gsk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4gsk RCSB], [https://www.ebi.ac.uk/pdbsum/4gsk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4gsk ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4gsk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gsk OCA], [https://pdbe.org/4gsk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4gsk RCSB], [https://www.ebi.ac.uk/pdbsum/4gsk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4gsk ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/ATG7_YEAST ATG7_YEAST] E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 and ATG8 for its conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes membranes.<ref>PMID:9759731</ref> <ref>PMID:8224160</ref> <ref>PMID:7593182</ref> <ref>PMID:10233148</ref> <ref>PMID:10233150</ref> <ref>PMID:11100732</ref> <ref>PMID:11149920</ref> <ref>PMID:12965207</ref> <ref>PMID:15277523</ref>		[https://www.uniprot.org/uniprot/ATG7_YEAST ATG7_YEAST] E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 and ATG8 for its conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes membranes.<ref>PMID:9759731</ref> <ref>PMID:8224160</ref> <ref>PMID:7593182</ref> <ref>PMID:10233148</ref> <ref>PMID:10233150</ref> <ref>PMID:11100732</ref> <ref>PMID:11149920</ref> <ref>PMID:12965207</ref> <ref>PMID:15277523</ref>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Core functions of autophagy are mediated by ubiquitin-like protein (UBL) cascades, in which a homodimeric E1 enzyme, Atg7, directs the UBLs Atg8 and Atg12 to their respective E2 enzymes, Atg3 and Atg10. Crystallographic and mutational analyses of yeast (Atg7-Atg3)(2) and (Atg7-Atg10)(2) complexes reveal noncanonical, multisite E1-E2 recognition in autophagy. Atg7's unique N-terminal domain recruits distinctive elements from the Atg3 and Atg10 'backsides'. This, along with E1 and E2 conformational variability, allows presentation of 'frontside' Atg3 and Atg10 active sites to the catalytic cysteine in the C-terminal domain from the opposite Atg7 protomer in the homodimer. Despite different modes of binding, the data suggest that common principles underlie conjugation in both noncanonical and canonical UBL cascades, whereby flexibly tethered E1 domains recruit E2s through surfaces remote from their active sites to juxtapose the E1 and E2 catalytic cysteines.
-
-	Noncanonical E2 recruitment by the autophagy E1 revealed by Atg7-Atg3 and Atg7-Atg10 structures.,Kaiser SE, Mao K, Taherbhoy AM, Yu S, Olszewski JL, Duda DM, Kurinov I, Deng A, Fenn TD, Klionsky DJ, Schulman BA Nat Struct Mol Biol. 2012 Nov 11. doi: 10.1038/nsmb.2415. PMID:23142976<ref>PMID:23142976</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 4gsk" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 07:15, 26 October 2022

OCA — Wed, 26 Oct 2022 07:15:09 GMT

←Older revision		Revision as of 07:15, 26 October 2022
Line 1:		Line 1:

	==Crystal structure of an Atg7-Atg10 crosslinked complex==		==Crystal structure of an Atg7-Atg10 crosslinked complex==
-	<StructureSection load='4gsk' size='340' side='right' caption='[[4gsk]], [[Resolution\|resolution]] 2.90Å' scene=''>	+	<StructureSection load='4gsk' size='340' side='right'caption='[[4gsk]], [[Resolution\|resolution]] 2.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4gsk]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Baker's_yeast Baker's yeast~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GSK OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4GSK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4gsk]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GSK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4GSK FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4gsj\|4gsj]], [[4gsl\|4gsl]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4gsk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gsk OCA], [https://pdbe.org/4gsk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4gsk RCSB], [https://www.ebi.ac.uk/pdbsum/4gsk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4gsk ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">APG7, ATG7, CVT2, YHR171W ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast]), APG10, ATG10, YLL042C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4gsk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gsk OCA], [~~http~~://pdbe.org/4gsk PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4gsk RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4gsk PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4gsk ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/ATG7_YEAST ATG7_YEAST]] E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 and ATG8 for its conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes membranes.<ref>PMID:9759731</ref> <ref>PMID:8224160</ref> <ref>PMID:7593182</ref> <ref>PMID:10233148</ref> <ref>PMID:10233150</ref> <ref>PMID:11100732</ref> <ref>PMID:11149920</ref> <ref>PMID:12965207</ref> <ref>PMID:15277523</ref> [[http://www.uniprot.org/uniprot/ATG10_YEAST ATG10_YEAST]] E2-like enzyme required for the cytoplasm to vacuole transport (Cvt), autophagy and nucleophagy. Acts as an E2-like enzyme that catalyzes the conjugation of ATG12 to ATG5. ATG12 conjugation to ATG5 is required for proper localization of ATG8 to the preautophagosomal structure (PAS). Likely serves as an ATG5-recognition molecule.	+	[https://www.uniprot.org/uniprot/ATG7_YEAST ATG7_YEAST] E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 and ATG8 for its conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes membranes.<ref>PMID:9759731</ref> <ref>PMID:8224160</ref> <ref>PMID:7593182</ref> <ref>PMID:10233148</ref> <ref>PMID:10233150</ref> <ref>PMID:11100732</ref> <ref>PMID:11149920</ref> <ref>PMID:12965207</ref> <ref>PMID:15277523</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 20:		Line 18:
	</div>		</div>
	<div class="pdbe-citations 4gsk" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4gsk" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Autophagy-related protein 3D structures\|Autophagy-related protein 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Baker's yeast~~]]	+	[[Category: Large Structures]]
-	[[Category: Deng, A]]	+	[[Category: Saccharomyces cerevisiae S288C]]
-	[[Category: Duda~~, D M~~]]	+	[[Category: Deng A]]
-	[[Category: Fenn~~, T D~~]]	+	[[Category: Duda DM]]
-	[[Category: Kaiser~~, S E~~]]	+	[[Category: Fenn TD]]
-	[[Category: Klionsky~~, D J~~]]	+	[[Category: Kaiser SE]]
-	[[Category: Kurinov, I]]	+	[[Category: Klionsky DJ]]
-	[[Category: Mao, K]]	+	[[Category: Kurinov I]]
-	[[Category: Olszewski~~, J L~~]]	+	[[Category: Mao K]]
-	[[Category: Schulman~~, B A~~]]	+	[[Category: Olszewski JL]]
-	[[Category: Taherbhoy~~, A M~~]]	+	[[Category: Schulman BA]]
-	[[Category: Yu, S]]	+	[[Category: Taherbhoy AM]]
-	~~[[Category: Protein transport-ligase complex]]~~	+	[[Category: Yu S]]
-	~~[[Category: Ubiquitin-like protein activation enzyme]]~~	+
-	~~[[Category: Ubiquitin-like protein transfer enzyme~~]]	+

OCA at 08:32, 15 November 2017

OCA — Wed, 15 Nov 2017 08:32:25 GMT

←Older revision		Revision as of 08:32, 15 November 2017
Line 20:		Line 20:
	</div>		</div>
	<div class="pdbe-citations 4gsk" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4gsk" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Autophagy-related protein\|Autophagy-related protein]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 12:52, 4 August 2016

OCA — Thu, 04 Aug 2016 12:52:02 GMT

←Older revision		Revision as of 12:52, 4 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of an Atg7-Atg10 crosslinked complex==		==Crystal structure of an Atg7-Atg10 crosslinked complex==
	<StructureSection load='4gsk' size='340' side='right' caption='[[4gsk]], [[Resolution\|resolution]] 2.90Å' scene=''>		<StructureSection load='4gsk' size='340' side='right' caption='[[4gsk]], [[Resolution\|resolution]] 2.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4gsk]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Saccharomyces_cerevisiae_s288c Saccharomyces cerevisiae s288c~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GSK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GSK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4gsk]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GSK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GSK FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4gsj\|4gsj]], [[4gsl\|4gsl]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4gsj\|4gsj]], [[4gsl\|4gsl]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">APG7, ATG7, CVT2, YHR171W ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 ~~Saccharomyces cerevisiae S288c~~]), APG10, ATG10, YLL042C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 ~~Saccharomyces cerevisiae S288c~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">APG7, ATG7, CVT2, YHR171W ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast]), APG10, ATG10, YLL042C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4gsk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gsk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4gsk RCSB], [http://www.ebi.ac.uk/pdbsum/4gsk PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4gsk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gsk OCA], [http://pdbe.org/4gsk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4gsk RCSB], [http://www.ebi.ac.uk/pdbsum/4gsk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4gsk ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4gsk" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Autophagy-related protein\|Autophagy-related protein]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Saccharomyces cerevisiae s288c~~]]	+	[[Category: Baker's yeast]]
	[[Category: Deng, A]]		[[Category: Deng, A]]
	[[Category: Duda, D M]]		[[Category: Duda, D M]]

OCA at 09:28, 25 December 2014

OCA — Thu, 25 Dec 2014 09:28:23 GMT

←Older revision		Revision as of 09:28, 25 December 2014
Line 1:		Line 1:
-	[[~~Image:~~4gsk.~~png~~\|~~left~~\|~~200px~~]]	+	==Crystal structure of an Atg7-Atg10 crosslinked complex==
		+	<StructureSection load='4gsk' size='340' side='right' caption='[[4gsk]], [[Resolution\|resolution]] 2.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4gsk]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_s288c Saccharomyces cerevisiae s288c]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GSK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GSK FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4gsj\|4gsj]], [[4gsl\|4gsl]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">APG7, ATG7, CVT2, YHR171W ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Saccharomyces cerevisiae S288c]), APG10, ATG10, YLL042C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Saccharomyces cerevisiae S288c])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4gsk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gsk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4gsk RCSB], [http://www.ebi.ac.uk/pdbsum/4gsk PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/ATG7_YEAST ATG7_YEAST]] E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 and ATG8 for its conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes membranes.<ref>PMID:9759731</ref> <ref>PMID:8224160</ref> <ref>PMID:7593182</ref> <ref>PMID:10233148</ref> <ref>PMID:10233150</ref> <ref>PMID:11100732</ref> <ref>PMID:11149920</ref> <ref>PMID:12965207</ref> <ref>PMID:15277523</ref> [[http://www.uniprot.org/uniprot/ATG10_YEAST ATG10_YEAST]] E2-like enzyme required for the cytoplasm to vacuole transport (Cvt), autophagy and nucleophagy. Acts as an E2-like enzyme that catalyzes the conjugation of ATG12 to ATG5. ATG12 conjugation to ATG5 is required for proper localization of ATG8 to the preautophagosomal structure (PAS). Likely serves as an ATG5-recognition molecule.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Core functions of autophagy are mediated by ubiquitin-like protein (UBL) cascades, in which a homodimeric E1 enzyme, Atg7, directs the UBLs Atg8 and Atg12 to their respective E2 enzymes, Atg3 and Atg10. Crystallographic and mutational analyses of yeast (Atg7-Atg3)(2) and (Atg7-Atg10)(2) complexes reveal noncanonical, multisite E1-E2 recognition in autophagy. Atg7's unique N-terminal domain recruits distinctive elements from the Atg3 and Atg10 'backsides'. This, along with E1 and E2 conformational variability, allows presentation of 'frontside' Atg3 and Atg10 active sites to the catalytic cysteine in the C-terminal domain from the opposite Atg7 protomer in the homodimer. Despite different modes of binding, the data suggest that common principles underlie conjugation in both noncanonical and canonical UBL cascades, whereby flexibly tethered E1 domains recruit E2s through surfaces remote from their active sites to juxtapose the E1 and E2 catalytic cysteines.

-	~~{{STRUCTURE_4gsk\| PDB=4gsk \| SCENE= }}~~	+	Noncanonical E2 recruitment by the autophagy E1 revealed by Atg7-Atg3 and Atg7-Atg10 structures.,Kaiser SE, Mao K, Taherbhoy AM, Yu S, Olszewski JL, Duda DM, Kurinov I, Deng A, Fenn TD, Klionsky DJ, Schulman BA Nat Struct Mol Biol. 2012 Nov 11. doi: 10.1038/nsmb.2415. PMID:23142976<ref>PMID:23142976</ref>

-	~~===Crystal structure~~ of ~~an Atg7-Atg10 crosslinked complex===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_23142976}}~~	+	== References ==
-		+	<references/>
-	==~~About this Structure~~==	+	__TOC__
-	~~[[4gsk]] is a 4 chain structure with sequence from [http:~~//~~en.wikipedia.org/wiki/Saccharomyces_cerevisiae_s288c Saccharomyces cerevisiae s288c]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GSK OCA].~~	+	</StructureSection>
	[[Category: Saccharomyces cerevisiae s288c]]		[[Category: Saccharomyces cerevisiae s288c]]
-	[[Category: Deng, A.]]	+	[[Category: Deng, A]]
-	[[Category: Duda, D M.]]	+	[[Category: Duda, D M]]
-	[[Category: Fenn, T D.]]	+	[[Category: Fenn, T D]]
-	[[Category: Kaiser, S E.]]	+	[[Category: Kaiser, S E]]
-	[[Category: Klionsky, D J.]]	+	[[Category: Klionsky, D J]]
-	[[Category: Kurinov, I.]]	+	[[Category: Kurinov, I]]
-	[[Category: Mao, K.]]	+	[[Category: Mao, K]]
-	[[Category: Olszewski, J L.]]	+	[[Category: Olszewski, J L]]
-	[[Category: Schulman, B A.]]	+	[[Category: Schulman, B A]]
-	[[Category: Taherbhoy, A M.]]	+	[[Category: Taherbhoy, A M]]
-	[[Category: Yu, S.]]	+	[[Category: Yu, S]]
	[[Category: Protein transport-ligase complex]]		[[Category: Protein transport-ligase complex]]
	[[Category: Ubiquitin-like protein activation enzyme]]		[[Category: Ubiquitin-like protein activation enzyme]]
	[[Category: Ubiquitin-like protein transfer enzyme]]		[[Category: Ubiquitin-like protein transfer enzyme]]

OCA at 14:30, 28 November 2012

OCA — Wed, 28 Nov 2012 14:30:12 GMT

←Older revision		Revision as of 14:30, 28 November 2012
Line 1:		Line 1:
-	[[Image:4gsk.~~jpg~~\|left\|200px]]	+	[[Image:4gsk.png\|left\|200px]]

	{{STRUCTURE_4gsk\| PDB=4gsk \| SCENE= }}		{{STRUCTURE_4gsk\| PDB=4gsk \| SCENE= }}
Line 5:		Line 5:
	===Crystal structure of an Atg7-Atg10 crosslinked complex===		===Crystal structure of an Atg7-Atg10 crosslinked complex===

		+	{{ABSTRACT_PUBMED_23142976}}

	==About this Structure==		==About this Structure==

OCA at 06:59, 14 November 2012

OCA — Wed, 14 Nov 2012 06:59:11 GMT

←Older revision		Revision as of 06:59, 14 November 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:4gsk.jpg\|left\|200px]]

-	~~The entry~~ 4gsk ~~is ON HOLD~~ ~~until Paper Publication~~	+	{{STRUCTURE_4gsk\| PDB=4gsk \| SCENE= }}

-	~~Authors: Kaiser, S.E., Mao, K., Taherbhoy, A.M., Yu, S., Olszewski, J.L., Duda, D.M., Kurinov, I., Deng, A., Fenn, T.D., Klionsky, D.J., Schulman, B.A.~~	+	===Crystal structure of an Atg7-Atg10 crosslinked complex===

-	~~Description: Crystal~~ structure ~~of an Atg7~~-~~Atg10 crosslinked~~ complex	+
		+	==About this Structure==
		+	[[4gsk]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_s288c Saccharomyces cerevisiae s288c]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GSK OCA].
		+	[[Category: Saccharomyces cerevisiae s288c]]
		+	[[Category: Deng, A.]]
		+	[[Category: Duda, D M.]]
		+	[[Category: Fenn, T D.]]
		+	[[Category: Kaiser, S E.]]
		+	[[Category: Klionsky, D J.]]
		+	[[Category: Kurinov, I.]]
		+	[[Category: Mao, K.]]
		+	[[Category: Olszewski, J L.]]
		+	[[Category: Schulman, B A.]]
		+	[[Category: Taherbhoy, A M.]]
		+	[[Category: Yu, S.]]
		+	[[Category: Protein transport-ligase complex]]
		+	[[Category: Ubiquitin-like protein activation enzyme]]
		+	[[Category: Ubiquitin-like protein transfer enzyme]]

OCA at 06:53, 10 October 2012

OCA — Wed, 10 Oct 2012 06:53:31 GMT

←Older revision		Revision as of 06:53, 10 October 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4gsk is ON HOLD	+	The entry 4gsk is ON HOLD until Paper Publication

	Authors: Kaiser, S.E., Mao, K., Taherbhoy, A.M., Yu, S., Olszewski, J.L., Duda, D.M., Kurinov, I., Deng, A., Fenn, T.D., Klionsky, D.J., Schulman, B.A.		Authors: Kaiser, S.E., Mao, K., Taherbhoy, A.M., Yu, S., Olszewski, J.L., Duda, D.M., Kurinov, I., Deng, A., Fenn, T.D., Klionsky, D.J., Schulman, B.A.

-	Description: ~~E1 E2~~ complex 2	+	Description: Crystal structure of an Atg7-Atg10 crosslinked complex

OCA: Protected "4gsk" [edit=sysop:move=sysop]

OCA — Wed, 05 Sep 2012 04:57:56 GMT

Protected "4gsk" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:57, 5 September 2012

OCA: New page: '''Unreleased structure''' The entry 4gsk is ON HOLD Authors: Kaiser, S.E., Mao, K., Taherbhoy, A.M., Yu, S., Olszewski, J.L., Duda, D.M., Kurinov, I., Deng, A., Fenn, T.D., Klionsky, D...

OCA — Wed, 05 Sep 2012 04:57:55 GMT

New page: '''Unreleased structure''' The entry 4gsk is ON HOLD Authors: Kaiser, S.E., Mao, K., Taherbhoy, A.M., Yu, S., Olszewski, J.L., Duda, D.M., Kurinov, I., Deng, A., Fenn, T.D., Klionsky, D...

New page

'''Unreleased structure'''

The entry 4gsk is ON HOLD

Authors: Kaiser, S.E., Mao, K., Taherbhoy, A.M., Yu, S., Olszewski, J.L., Duda, D.M., Kurinov, I., Deng, A., Fenn, T.D., Klionsky, D.J., Schulman, B.A.

Description: E1 E2 complex 2