4gx8 - Revision history

OCA at 14:02, 8 November 2023

OCA — Wed, 08 Nov 2023 14:02:44 GMT

←Older revision		Revision as of 14:02, 8 November 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4gx8]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GX8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4GX8 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4gx8]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GX8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4GX8 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.7Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4gx8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gx8 OCA], [https://pdbe.org/4gx8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4gx8 RCSB], [https://www.ebi.ac.uk/pdbsum/4gx8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4gx8 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4gx8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gx8 OCA], [https://pdbe.org/4gx8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4gx8 RCSB], [https://www.ebi.ac.uk/pdbsum/4gx8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4gx8 ProSAT]</span></td></tr>
	</table>		</table>
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	</div>		</div>
	<div class="pdbe-citations 4gx8" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4gx8" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[DNA polymerase 3D structures\|DNA polymerase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 07:24, 26 October 2022

OCA — Wed, 26 Oct 2022 07:24:18 GMT

←Older revision		Revision as of 07:24, 26 October 2022
Line 3:		Line 3:
	<StructureSection load='4gx8' size='340' side='right'caption='[[4gx8]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='4gx8' size='340' side='right'caption='[[4gx8]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4gx8]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GX8 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4GX8 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4gx8]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GX8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4GX8 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2hnh\|2hnh]], [[2hqa\|2hqa]], [[1j53\|1j53]], [[1j54\|1j54]], [[2ido\|2ido]], [[2gui\|2gui]], [[2axd\|2axd]], [[4gx9\|4gx9]]</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4gx8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gx8 OCA], [https://pdbe.org/4gx8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4gx8 RCSB], [https://www.ebi.ac.uk/pdbsum/4gx8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4gx8 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4gx8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gx8 OCA], [~~http~~://pdbe.org/4gx8 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4gx8 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4gx8 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4gx8 ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/DPO3E_ECOLI DPO3E_ECOLI]] DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. The epsilon subunit contain the editing function and is a proofreading 3'-5' exonuclease.	+	[https://www.uniprot.org/uniprot/DPO3A_ECOLI DPO3A_ECOLI] DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The alpha chain is the DNA polymerase.[https://www.uniprot.org/uniprot/DPO3E_ECOLI DPO3E_ECOLI] DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. The epsilon subunit contain the editing function and is a proofreading 3'-5' exonuclease.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 4gx8" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4gx8" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[DNA polymerase 3D structures\|DNA polymerase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Escherichia coli K-12]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Dixon~~, N E~~]]	+	[[Category: Dixon NE]]
-	[[Category: Horan, N]]	+	[[Category: Horan N]]
-	[[Category: Oakley~~, A J~~]]	+	[[Category: Oakley AJ]]
-	[[Category: Robinson, A]]	+	[[Category: Robinson A]]
-	[[Category: Xu, Z Q]]	+	[[Category: Xu Z-Q]]
-	~~[[Category: Dna polymerase iii]]~~	+
-	~~[[Category: Dnae]]~~	+
-	~~[[Category: Dnaq]]~~	+
-	~~[[Category: Poliii alpha]]~~	+
-	~~[[Category: Poliii epsilon]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 09:22, 5 February 2020

OCA — Wed, 05 Feb 2020 09:22:14 GMT

←Older revision		Revision as of 09:22, 5 February 2020
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	==Crystal structure of a DNA polymerase III alpha-epsilon chimera==		==Crystal structure of a DNA polymerase III alpha-epsilon chimera==
-	<StructureSection load='4gx8' size='340' side='right' caption='[[4gx8]], [[Resolution\|resolution]] 1.70Å' scene=''>	+	<StructureSection load='4gx8' size='340' side='right'caption='[[4gx8]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4gx8]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GX8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GX8 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4gx8]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GX8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GX8 FirstGlance]. <br>
Line 19:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 4gx8" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4gx8" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[DNA polymerase 3D structures\|DNA polymerase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Dixon, N E]]		[[Category: Dixon, N E]]
	[[Category: Horan, N]]		[[Category: Horan, N]]

OCA at 09:20, 16 November 2017

OCA — Thu, 16 Nov 2017 09:20:59 GMT

←Older revision		Revision as of 09:20, 16 November 2017
Line 19:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 4gx8" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4gx8" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[DNA polymerase\|DNA polymerase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 10:16, 3 August 2017

OCA — Thu, 03 Aug 2017 10:16:29 GMT

←Older revision		Revision as of 10:16, 3 August 2017
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	<StructureSection load='4gx8' size='340' side='right' caption='[[4gx8]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='4gx8' size='340' side='right' caption='[[4gx8]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4gx8]] is a 4 chain structure ~~with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GX8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GX8 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4gx8]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GX8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GX8 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2hnh\|2hnh]], [[2hqa\|2hqa]], [[1j53\|1j53]], [[1j54\|1j54]], [[2ido\|2ido]], [[2gui\|2gui]], [[2axd\|2axd]], [[4gx9\|4gx9]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2hnh\|2hnh]], [[2hqa\|2hqa]], [[1j53\|1j53]], [[1j54\|1j54]], [[2ido\|2ido]], [[2gui\|2gui]], [[2axd\|2axd]], [[4gx9\|4gx9]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b0184, b0215, dnaE, dnaQ, JW0179, JW0205, mutD, polC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4gx8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gx8 OCA], [http://pdbe.org/4gx8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4gx8 RCSB], [http://www.ebi.ac.uk/pdbsum/4gx8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4gx8 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4gx8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gx8 OCA], [http://pdbe.org/4gx8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4gx8 RCSB], [http://www.ebi.ac.uk/pdbsum/4gx8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4gx8 ProSAT]</span></td></tr>
	</table>		</table>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: DNA-directed DNA polymerase]]
-	[[Category: Ecoli]]
	[[Category: Dixon, N E]]		[[Category: Dixon, N E]]
	[[Category: Horan, N]]		[[Category: Horan, N]]

OCA at 22:44, 4 August 2016

OCA — Thu, 04 Aug 2016 22:44:01 GMT

←Older revision		Revision as of 22:44, 4 August 2016
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		+
	==Crystal structure of a DNA polymerase III alpha-epsilon chimera==		==Crystal structure of a DNA polymerase III alpha-epsilon chimera==
	<StructureSection load='4gx8' size='340' side='right' caption='[[4gx8]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='4gx8' size='340' side='right' caption='[[4gx8]], [[Resolution\|resolution]] 1.70Å' scene=''>
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	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b0184, b0215, dnaE, dnaQ, JW0179, JW0205, mutD, polC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b0184, b0215, dnaE, dnaQ, JW0179, JW0205, mutD, polC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4gx8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gx8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4gx8 RCSB], [http://www.ebi.ac.uk/pdbsum/4gx8 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4gx8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gx8 OCA], [http://pdbe.org/4gx8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4gx8 RCSB], [http://www.ebi.ac.uk/pdbsum/4gx8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4gx8 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 19:		Line 20:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4gx8" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 13:43, 25 December 2014

OCA — Thu, 25 Dec 2014 13:43:24 GMT

←Older revision		Revision as of 13:43, 25 December 2014
Line 9:		Line 9:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4gx8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gx8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4gx8 RCSB], [http://www.ebi.ac.uk/pdbsum/4gx8 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4gx8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gx8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4gx8 RCSB], [http://www.ebi.ac.uk/pdbsum/4gx8 PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/DPO3E_ECOLI DPO3E_ECOLI]] DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. The epsilon subunit contain the editing function and is a proofreading 3'-5' exonuclease.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 10:47, 21 December 2014

OCA — Sun, 21 Dec 2014 10:47:45 GMT

←Older revision		Revision as of 10:47, 21 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_4gx8\| PDB=4gx8 \| SCENE= }}~~	+	==Crystal structure of a DNA polymerase III alpha-epsilon chimera==
-	===Crystal structure of a DNA polymerase III alpha-epsilon chimera===	+	<StructureSection load='4gx8' size='340' side='right' caption='[[4gx8]], [[Resolution\|resolution]] 1.70Å' scene=''>
-	~~{{ABSTRACT_PUBMED_23580545}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4gx8]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GX8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GX8 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2hnh\|2hnh]], [[2hqa\|2hqa]], [[1j53\|1j53]], [[1j54\|1j54]], [[2ido\|2ido]], [[2gui\|2gui]], [[2axd\|2axd]], [[4gx9\|4gx9]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b0184, b0215, dnaE, dnaQ, JW0179, JW0205, mutD, polC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4gx8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gx8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4gx8 RCSB], [http://www.ebi.ac.uk/pdbsum/4gx8 PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	A complex of the three (alphaepsilontheta) core subunits and the beta2 sliding clamp is responsible for DNA synthesis by Pol III, the Escherichia coli chromosomal DNA replicase. The 1.7 A crystal structure of a complex between the PHP domain of alpha (polymerase) and the C-terminal segment of epsilon (proofreading exonuclease) subunits shows that epsilon is attached to alpha at a site far from the polymerase active site. Both alpha and epsilon contain clamp-binding motifs (CBMs) that interact simultaneously with beta2 in the polymerization mode of DNA replication by Pol III. Strengthening of both CBMs enables isolation of stable alphaepsilontheta:beta2 complexes. Nuclear magnetic resonance experiments with reconstituted alphaepsilontheta:beta2 demonstrate retention of high mobility of a segment of 22 residues in the linker that connects the exonuclease domain of epsilon with its alpha-binding segment. In spite of this, small-angle X-ray scattering data show that the isolated complex with strengthened CBMs has a compact, but still flexible, structure. Photo-crosslinking with p-benzoyl-L-phenylalanine incorporated at different sites in the alpha-PHP domain confirm the conformational variability of the tether. Structural models of the alphaepsilontheta:beta2 replicase complex with primer-template DNA combine all available structural data.

-	~~==Function==~~	+	Proofreading exonuclease on a tether: the complex between the E. coli DNA polymerase III subunits alpha, {varepsilon}, theta and beta reveals a highly flexible arrangement of the proofreading domain.,Ozawa K, Horan NP, Robinson A, Yagi H, Hill FR, Jergic S, Xu ZQ, Loscha KV, Li N, Tehei M, Oakley AJ, Otting G, Huber T, Dixon NE Nucleic Acids Res. 2013 Apr 10. PMID:23580545<ref>PMID:23580545</ref>
-	~~[[http~~:~~//www~~.~~uniprot.org/uniprot/DPO3E_ECOLI DPO3E_ECOLI]]~~ DNA polymerase III ~~is a complex~~, ~~multichain enzyme responsible for most of the replicative synthesis in bacteria. The epsilon subunit contain the editing function~~ and is a proofreading ~~3'-5' exonuclease~~.	+

-	~~==About this Structure==~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[4gx8]] is~~ a ~~4 chain structure~~. ~~Full crystallographic information is available from [http://oca~~.~~weizmann~~.~~ac.il~~/~~oca-bin/ocashort?id=4GX8 OCA].~~	+	</div>

-	==~~Reference~~==	+	==See Also==
-	~~<ref group~~=~~"xtra">PMID:023580545</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	*[[DNA polymerase\|DNA polymerase]]
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: DNA-directed DNA polymerase]]		[[Category: DNA-directed DNA polymerase]]
-	[[Category: Dixon, N E.]]	+	[[Category: Ecoli]]
-	[[Category: Horan, N.]]	+	[[Category: Dixon, N E]]
-	[[Category: Oakley, A J.]]	+	[[Category: Horan, N]]
-	[[Category: Robinson, A.]]	+	[[Category: Oakley, A J]]
-	[[Category: Xu, Z Q.]]	+	[[Category: Robinson, A]]
		+	[[Category: Xu, Z Q]]
	[[Category: Dna polymerase iii]]		[[Category: Dna polymerase iii]]
	[[Category: Dnae]]		[[Category: Dnae]]

OCA at 07:12, 30 June 2013

OCA — Sun, 30 Jun 2013 07:12:59 GMT

←Older revision		Revision as of 07:12, 30 June 2013
Line 8:		Line 8:
	==About this Structure==		==About this Structure==
	[[4gx8]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GX8 OCA].		[[4gx8]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GX8 OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:023580545</ref><references group="xtra"/><references/>
	[[Category: DNA-directed DNA polymerase]]		[[Category: DNA-directed DNA polymerase]]
	[[Category: Dixon, N E.]]		[[Category: Dixon, N E.]]

OCA at 10:20, 24 April 2013

OCA — Wed, 24 Apr 2013 10:20:29 GMT

←Older revision		Revision as of 10:20, 24 April 2013
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	{{STRUCTURE_4gx8\| PDB=4gx8 \| SCENE= }}		{{STRUCTURE_4gx8\| PDB=4gx8 \| SCENE= }}
	===Crystal structure of a DNA polymerase III alpha-epsilon chimera===		===Crystal structure of a DNA polymerase III alpha-epsilon chimera===
-	{{~~ABSTRACT_PUBMED_18663010~~}}	+	{{ABSTRACT_PUBMED_23580545}}

	==Function==		==Function==