4h0o - Revision history

OCA at 16:01, 14 March 2024

OCA — Thu, 14 Mar 2024 16:01:23 GMT

←Older revision		Revision as of 16:01, 14 March 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4h0o]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Entamoeba_histolytica Entamoeba histolytica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H0O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4H0O FirstGlance]. <br>		<table><tr><td colspan='2'>[[4h0o]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Entamoeba_histolytica Entamoeba histolytica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H0O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4H0O FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4h0o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4h0o OCA], [https://pdbe.org/4h0o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4h0o RCSB], [https://www.ebi.ac.uk/pdbsum/4h0o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4h0o ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.4Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4h0o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4h0o OCA], [https://pdbe.org/4h0o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4h0o RCSB], [https://www.ebi.ac.uk/pdbsum/4h0o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4h0o ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/C4M1C3_ENTHI C4M1C3_ENTHI]		[https://www.uniprot.org/uniprot/C4M1C3_ENTHI C4M1C3_ENTHI]
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Acetate kinases (ACKs) are members of the acetate and sugar kinase/hsp70/actin (ASKHA) superfamily and catalyze the reversible phosphorylation of acetate, with ADP/ATP the most common phosphoryl acceptor/donor. While prokaryotic ACKs have been the subject of extensive biochemical and structural characterization, there is a comparative paucity of information on eukaryotic ACKs, and prior to this report, no structure of an ACK of eukaryotic origin was available. We determined the structures of ACKs from the eukaryotic pathogens Entamoeba histolytica and Cryptococcus neoformans. Each active site is located at an interdomain interface, and the acetate and phosphate binding pockets display sequence and structural conservation with their prokaryotic counterparts. Interestingly, the E. histolytica ACK has previously been shown to be pyrophosphate (PP(i))-dependent, and is the first ACK demonstrated to have this property. Examination of its structure demonstrates how subtle amino acid substitutions within the active site have converted cosubstrate specificity from ATP to PP(i) while retaining a similar backbone conformation. Differences in the angle between domains surrounding the active site suggest that interdomain movement may accompany catalysis. Taken together, these structures are consistent with the eukaryotic ACKs following a similar reaction mechanism as is proposed for the prokaryotic homologs.
-
-	Crystal structures of acetate kinases from the eukaryotic pathogens Entamoeba histolytica and Cryptococcus neoformans.,Thaker TM, Tanabe M, Fowler ML, Preininger AM, Ingram-Smith C, Smith KS, Iverson TM J Struct Biol. 2012 Nov 16. pii: S1047-8477(12)00313-9. doi:, 10.1016/j.jsb.2012.11.001. PMID:23159802<ref>PMID:23159802</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 4h0o" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 07:51, 3 November 2022

OCA — Thu, 03 Nov 2022 07:51:57 GMT

←Older revision		Revision as of 07:51, 3 November 2022
Line 1:		Line 1:

	==Crystal Structure of Acetate Kinase from Entamoeba histolytica==		==Crystal Structure of Acetate Kinase from Entamoeba histolytica==
-	<StructureSection load='4h0o' size='340' side='right' caption='[[4h0o]], [[Resolution\|resolution]] 2.40Å' scene=''>	+	<StructureSection load='4h0o' size='340' side='right'caption='[[4h0o]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4h0o]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Enthi Enthi~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H0O OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4H0O FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4h0o]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Entamoeba_histolytica Entamoeba histolytica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H0O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4H0O FirstGlance]. <br>
-	</td></tr>~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4h0p\|4h0p]]</td></tr>~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4h0o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4h0o OCA], [https://pdbe.org/4h0o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4h0o RCSB], [https://www.ebi.ac.uk/pdbsum/4h0o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4h0o ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ACKA, EHI_170010 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5759 ENTHI])</td></tr>	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Acetate_kinase_(diphosphate) Acetate kinase (diphosphate)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.2.12 2.7.2.12] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4h0o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4h0o OCA], [~~http~~://pdbe.org/4h0o PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4h0o RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4h0o PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4h0o ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/C4M1C3_ENTHI C4M1C3_ENTHI]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Enthi~~]]	+	[[Category: Entamoeba histolytica]]
-	[[Category: ~~Iverson, T M~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Tanabe, M~~]]	+	[[Category: Iverson TM]]
-	[[Category: ~~Thaker, T~~ M]]	+	[[Category: Tanabe M]]
-	[[Category: ~~Hsc70]]~~	+	[[Category: Thaker TM]]
-	~~[[Category: Pyrophosphate-dependent acetate kinase]]~~	+
-	~~[[Category: Ribonuclease h-like fold]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 10:52, 11 August 2016

OCA — Thu, 11 Aug 2016 10:52:17 GMT

←Older revision		Revision as of 10:52, 11 August 2016
Line 1:		Line 1:
		+
	==Crystal Structure of Acetate Kinase from Entamoeba histolytica==		==Crystal Structure of Acetate Kinase from Entamoeba histolytica==
	<StructureSection load='4h0o' size='340' side='right' caption='[[4h0o]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='4h0o' size='340' side='right' caption='[[4h0o]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4h0o]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Entamoeba_histolytica Entamoeba histolytica~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H0O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4H0O FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4h0o]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Enthi Enthi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H0O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4H0O FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4h0p\|4h0p]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4h0p\|4h0p]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ACKA, EHI_170010 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5759 ~~Entamoeba histolytica~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ACKA, EHI_170010 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5759 ENTHI])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Acetate_kinase_(diphosphate) Acetate kinase (diphosphate)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.2.12 2.7.2.12] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Acetate_kinase_(diphosphate) Acetate kinase (diphosphate)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.2.12 2.7.2.12] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4h0o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4h0o OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4h0o RCSB], [http://www.ebi.ac.uk/pdbsum/4h0o PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4h0o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4h0o OCA], [http://pdbe.org/4h0o PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4h0o RCSB], [http://www.ebi.ac.uk/pdbsum/4h0o PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4h0o ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4h0o" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Entamoeba histolytica~~]]	+	[[Category: Enthi]]
	[[Category: Iverson, T M]]		[[Category: Iverson, T M]]
	[[Category: Tanabe, M]]		[[Category: Tanabe, M]]

OCA at 12:32, 4 January 2015

OCA — Sun, 04 Jan 2015 12:32:12 GMT

←Older revision		Revision as of 12:32, 4 January 2015
Line 1:		Line 1:
-	[[~~Image:~~4h0o.~~png~~\|~~left~~\|~~200px~~]]	+	==Crystal Structure of Acetate Kinase from Entamoeba histolytica==
		+	<StructureSection load='4h0o' size='340' side='right' caption='[[4h0o]], [[Resolution\|resolution]] 2.40Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4h0o]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Entamoeba_histolytica Entamoeba histolytica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H0O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4H0O FirstGlance]. <br>
		+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4h0p\|4h0p]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ACKA, EHI_170010 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5759 Entamoeba histolytica])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Acetate_kinase_(diphosphate) Acetate kinase (diphosphate)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.2.12 2.7.2.12] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4h0o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4h0o OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4h0o RCSB], [http://www.ebi.ac.uk/pdbsum/4h0o PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Acetate kinases (ACKs) are members of the acetate and sugar kinase/hsp70/actin (ASKHA) superfamily and catalyze the reversible phosphorylation of acetate, with ADP/ATP the most common phosphoryl acceptor/donor. While prokaryotic ACKs have been the subject of extensive biochemical and structural characterization, there is a comparative paucity of information on eukaryotic ACKs, and prior to this report, no structure of an ACK of eukaryotic origin was available. We determined the structures of ACKs from the eukaryotic pathogens Entamoeba histolytica and Cryptococcus neoformans. Each active site is located at an interdomain interface, and the acetate and phosphate binding pockets display sequence and structural conservation with their prokaryotic counterparts. Interestingly, the E. histolytica ACK has previously been shown to be pyrophosphate (PP(i))-dependent, and is the first ACK demonstrated to have this property. Examination of its structure demonstrates how subtle amino acid substitutions within the active site have converted cosubstrate specificity from ATP to PP(i) while retaining a similar backbone conformation. Differences in the angle between domains surrounding the active site suggest that interdomain movement may accompany catalysis. Taken together, these structures are consistent with the eukaryotic ACKs following a similar reaction mechanism as is proposed for the prokaryotic homologs.

-	~~{{STRUCTURE_4h0o\| PDB=4h0o \| SCENE= }}~~	+	Crystal structures of acetate kinases from the eukaryotic pathogens Entamoeba histolytica and Cryptococcus neoformans.,Thaker TM, Tanabe M, Fowler ML, Preininger AM, Ingram-Smith C, Smith KS, Iverson TM J Struct Biol. 2012 Nov 16. pii: S1047-8477(12)00313-9. doi:, 10.1016/j.jsb.2012.11.001. PMID:23159802<ref>PMID:23159802</ref>

-	~~===Crystal Structure~~ of ~~Acetate Kinase from Entamoeba histolytica===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_23159802}}~~	+	== References ==
-		+	<references/>
-	==~~About this Structure~~==	+	__TOC__
-	~~[[4h0o]] is a 2 chain structure with sequence from [http:~~//~~en.wikipedia.org/wiki/Entamoeba_histolytica Entamoeba histolytica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H0O OCA].~~	+	</StructureSection>
	[[Category: Entamoeba histolytica]]		[[Category: Entamoeba histolytica]]
-	[[Category: Iverson, T M.]]	+	[[Category: Iverson, T M]]
-	[[Category: Tanabe, M.]]	+	[[Category: Tanabe, M]]
-	[[Category: Thaker, T M.]]	+	[[Category: Thaker, T M]]
	[[Category: Hsc70]]		[[Category: Hsc70]]
	[[Category: Pyrophosphate-dependent acetate kinase]]		[[Category: Pyrophosphate-dependent acetate kinase]]
	[[Category: Ribonuclease h-like fold]]		[[Category: Ribonuclease h-like fold]]
	[[Category: Transferase]]		[[Category: Transferase]]

OCA at 13:03, 20 February 2013

OCA — Wed, 20 Feb 2013 13:03:35 GMT

←Older revision		Revision as of 13:03, 20 February 2013
Line 1:		Line 1:
-	[[Image:4h0o.~~jpg~~\|left\|200px]]	+	[[Image:4h0o.png\|left\|200px]]

	{{STRUCTURE_4h0o\| PDB=4h0o \| SCENE= }}		{{STRUCTURE_4h0o\| PDB=4h0o \| SCENE= }}

OCA at 10:53, 5 December 2012

OCA — Wed, 05 Dec 2012 10:53:34 GMT

←Older revision		Revision as of 10:53, 5 December 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:4h0o.jpg\|left\|200px]]

-	~~The entry~~ 4h0o ~~is ON HOLD~~ ~~until Paper Publication~~	+	{{STRUCTURE_4h0o\| PDB=4h0o \| SCENE= }}

-	~~Authors: Thaker, T.M., Tanabe, M., Iverson, T.M.~~	+	===Crystal Structure of Acetate Kinase from Entamoeba histolytica===

-	~~Description: Crystal~~ Structure ~~of Acetate Kinase~~ from Entamoeba histolytica	+	{{ABSTRACT_PUBMED_23159802}}
		+
		+	==About this Structure==
		+	[[4h0o]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Entamoeba_histolytica Entamoeba histolytica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H0O OCA].
		+	[[Category: Entamoeba histolytica]]
		+	[[Category: Iverson, T M.]]
		+	[[Category: Tanabe, M.]]
		+	[[Category: Thaker, T M.]]
		+	[[Category: Hsc70]]
		+	[[Category: Pyrophosphate-dependent acetate kinase]]
		+	[[Category: Ribonuclease h-like fold]]
		+	[[Category: Transferase]]

OCA at 05:46, 3 October 2012

OCA — Wed, 03 Oct 2012 05:46:24 GMT

←Older revision		Revision as of 05:46, 3 October 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4h0o is ON HOLD	+	The entry 4h0o is ON HOLD until Paper Publication

	Authors: Thaker, T.M., Tanabe, M., Iverson, T.M.		Authors: Thaker, T.M., Tanabe, M., Iverson, T.M.

-	Description: Crystal Structure of ~~Apo Form~~ Acetate Kinase from Entamoeba histolytica	+	Description: Crystal Structure of Acetate Kinase from Entamoeba histolytica

OCA: Protected "4h0o" [edit=sysop:move=sysop]

OCA — Wed, 19 Sep 2012 06:56:46 GMT

Protected "4h0o" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:56, 19 September 2012

OCA: New page: '''Unreleased structure''' The entry 4h0o is ON HOLD Authors: Thaker, T.M., Tanabe, M., Iverson, T.M. Description: Crystal Structure of Apo Form Acetate Kinase from Entamoeba histolyti...

OCA — Wed, 19 Sep 2012 06:56:45 GMT

New page: '''Unreleased structure''' The entry 4h0o is ON HOLD Authors: Thaker, T.M., Tanabe, M., Iverson, T.M. Description: Crystal Structure of Apo Form Acetate Kinase from Entamoeba histolyti...

New page

'''Unreleased structure'''

The entry 4h0o is ON HOLD

Authors: Thaker, T.M., Tanabe, M., Iverson, T.M.

Description: Crystal Structure of Apo Form Acetate Kinase from Entamoeba histolytica