4h1g - Revision history

OCA at 16:01, 14 March 2024

OCA — Thu, 14 Mar 2024 16:01:47 GMT

←Older revision		Revision as of 16:01, 14 March 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4h1g]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Candida_albicans_WO-1 Candida albicans WO-1] and [https://en.wikipedia.org/wiki/Escherichia_coli_MS_21-1 Escherichia coli MS 21-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H1G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4H1G FirstGlance]. <br>		<table><tr><td colspan='2'>[[4h1g]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Candida_albicans_WO-1 Candida albicans WO-1] and [https://en.wikipedia.org/wiki/Escherichia_coli_MS_21-1 Escherichia coli MS 21-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H1G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4H1G FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PRD_900010:alpha-maltotetraose'>PRD_900010</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.15Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PRD_900010:alpha-maltotetraose'>PRD_900010</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4h1g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4h1g OCA], [https://pdbe.org/4h1g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4h1g RCSB], [https://www.ebi.ac.uk/pdbsum/4h1g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4h1g ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4h1g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4h1g OCA], [https://pdbe.org/4h1g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4h1g RCSB], [https://www.ebi.ac.uk/pdbsum/4h1g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4h1g ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/MALE_ECOLI MALE_ECOLI] Involved in the high-affinity maltose membrane transport system MalEFGK. Initial receptor for the active transport of and chemotaxis toward maltooligosaccharides.[https://www.uniprot.org/uniprot/C4YKK8_CANAW C4YKK8_CANAW]		[https://www.uniprot.org/uniprot/MALE_ECOLI MALE_ECOLI] Involved in the high-affinity maltose membrane transport system MalEFGK. Initial receptor for the active transport of and chemotaxis toward maltooligosaccharides.[https://www.uniprot.org/uniprot/C4YKK8_CANAW C4YKK8_CANAW]
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	In the human fungal pathogen Candida albicans, the Kinesin-14 motor protein Kar3 (CaKar3) is critical for normal mitotic division, nuclear fusion during mating, and morphogenic transition from the commensal yeast form to the virulent hyphal form. As a first step towards detailed characterization of this motor of potential medical significance, we have crystallized and determined the X-ray structure of the motor domain of CaKar3 as a maltose-binding protein (MBP) fusion. The structure shows strong conservation of overall motor domain topology to other Kar3 kinesins, but with some prominent differences in one of the motifs that compose the nucleotide-binding pocket and the surface charge distribution. The MBP and Kar3 modules are arranged such that MBP interacts with the Kar3 motor domain core at the same site where the neck linker of conventional kinesins docks during the "ATP state" of the mechanochemical cycle. This site differs from the Kar3 neck-core interface in the recent structure of the ScKar3Vik1 heterodimer. The position of MBP is also completely distinct from the Vik1 subunit in this complex. This may suggest that the site of MBP interaction on the CaKar3 motor domain provides an interface for the neck, or perhaps a partner subunit, at an intermediate state of its motile cycle that has not yet been observed for Kinesin-14 motors.
-
-	Crystal structure of the Candida albicans Kar3 kinesin motor domain fused to maltose-binding protein.,Delorme C, Joshi M, Allingham JS Biochem Biophys Res Commun. 2012 Nov 30;428(4):427-32. doi:, 10.1016/j.bbrc.2012.10.101. Epub 2012 Nov 5. PMID:23137538<ref>PMID:23137538</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 4h1g" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 07:52, 3 November 2022

OCA — Thu, 03 Nov 2022 07:52:41 GMT

←Older revision		Revision as of 07:52, 3 November 2022
Line 3:		Line 3:
	<StructureSection load='4h1g' size='340' side='right'caption='[[4h1g]], [[Resolution\|resolution]] 2.15Å' scene=''>		<StructureSection load='4h1g' size='340' side='right'caption='[[4h1g]], [[Resolution\|resolution]] 2.15Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4h1g]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Ecoli Ecoli~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H1G OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4H1G FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4h1g]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Candida_albicans_WO-1 Candida albicans WO-1] and [https://en.wikipedia.org/wiki/Escherichia_coli_MS_21-1 Escherichia coli MS 21-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H1G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4H1G FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:~~MAGNESIUM+ION~~'>MG</scene>, <scene name='pdbligand=~~MTT~~:~~MALTOTETRAOSE~~'>~~MTT~~</scene><~~/td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PRD_900010:alpha-maltotetraose'>PRD_900010</scene></td></tr>
-	~~<tr id~~='~~related'><td class~~=~~"sblockLbl"><b>[[Related_structure\|Related~~:~~]]</b~~></td>~~<td class="sblockDat">[[3kar\|3kar]], [[3t0q\|3t0q]], [[4gkr\|4gkr]]~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4h1g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4h1g OCA], [https://pdbe.org/4h1g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4h1g RCSB], [https://www.ebi.ac.uk/pdbsum/4h1g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4h1g ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4h1g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4h1g OCA], [~~http~~://pdbe.org/4h1g PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4h1g RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4h1g PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4h1g ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/MALE_ECOLI MALE_ECOLI] Involved in the high-affinity maltose membrane transport system MalEFGK. Initial receptor for the active transport of and chemotaxis toward maltooligosaccharides.[https://www.uniprot.org/uniprot/C4YKK8_CANAW C4YKK8_CANAW]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Ecoli~~]]	+	[[Category: Candida albicans WO-1]]
		+	[[Category: Escherichia coli MS 21-1]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Allingham~~, J A~~]]	+	[[Category: Allingham JA]]
-	[[Category: Delorme, C]]	+	[[Category: Delorme C]]
-	[[Category: Duan, D]]	+	[[Category: Duan D]]
-	[[Category: Joshi, M]]	+	[[Category: Joshi M]]
-	~~[[Category: Chimera]]~~	+
-	~~[[Category: Kinesin motor domain]]~~	+
-	~~[[Category: Motor protein~~]]	+

OCA at 09:47, 5 February 2020

OCA — Wed, 05 Feb 2020 09:47:00 GMT

←Older revision		Revision as of 09:47, 5 February 2020
Line 1:		Line 1:

	==Structure of Candida albicans Kar3 motor domain fused to maltose-binding protein==		==Structure of Candida albicans Kar3 motor domain fused to maltose-binding protein==
-	<StructureSection load='4h1g' size='340' side='right' caption='[[4h1g]], [[Resolution\|resolution]] 2.15Å' scene=''>	+	<StructureSection load='4h1g' size='340' side='right'caption='[[4h1g]], [[Resolution\|resolution]] 2.15Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4h1g]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H1G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4H1G FirstGlance]. <br>		<table><tr><td colspan='2'>[[4h1g]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H1G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4H1G FirstGlance]. <br>
Line 22:		Line 22:
	</StructureSection>		</StructureSection>
	[[Category: Ecoli]]		[[Category: Ecoli]]
		+	[[Category: Large Structures]]
	[[Category: Allingham, J A]]		[[Category: Allingham, J A]]
	[[Category: Delorme, C]]		[[Category: Delorme, C]]

OCA at 02:45, 16 November 2017

OCA — Thu, 16 Nov 2017 02:45:15 GMT

←Older revision		Revision as of 02:45, 16 November 2017
Line 3:		Line 3:
	<StructureSection load='4h1g' size='340' side='right' caption='[[4h1g]], [[Resolution\|resolution]] 2.15Å' scene=''>		<StructureSection load='4h1g' size='340' side='right' caption='[[4h1g]], [[Resolution\|resolution]] 2.15Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4h1g]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Canaw Canaw~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H1G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4H1G FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4h1g]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H1G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4H1G FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MTT:MALTOTETRAOSE'>MTT</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MTT:MALTOTETRAOSE'>MTT</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3kar\|3kar]], [[3t0q\|3t0q]], [[4gkr\|4gkr]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3kar\|3kar]], [[3t0q\|3t0q]], [[4gkr\|4gkr]]</td></tr>
Line 21:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Canaw~~]]	+	[[Category: Ecoli]]
	[[Category: Allingham, J A]]		[[Category: Allingham, J A]]
	[[Category: Delorme, C]]		[[Category: Delorme, C]]

OCA at 20:08, 11 August 2016

OCA — Thu, 11 Aug 2016 20:08:26 GMT

←Older revision		Revision as of 20:08, 11 August 2016
Line 1:		Line 1:
		+
	==Structure of Candida albicans Kar3 motor domain fused to maltose-binding protein==		==Structure of Candida albicans Kar3 motor domain fused to maltose-binding protein==
	<StructureSection load='4h1g' size='340' side='right' caption='[[4h1g]], [[Resolution\|resolution]] 2.15Å' scene=''>		<StructureSection load='4h1g' size='340' side='right' caption='[[4h1g]], [[Resolution\|resolution]] 2.15Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4h1g]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Candida_albicans_wo-1 Candida albicans wo-1~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H1G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4H1G FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4h1g]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Canaw Canaw]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H1G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4H1G FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MTT:MALTOTETRAOSE'>MTT</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MTT:MALTOTETRAOSE'>MTT</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3kar\|3kar]], [[3t0q\|3t0q]], [[4gkr\|4gkr]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3kar\|3kar]], [[3t0q\|3t0q]], [[4gkr\|4gkr]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4h1g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4h1g OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4h1g RCSB], [http://www.ebi.ac.uk/pdbsum/4h1g PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4h1g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4h1g OCA], [http://pdbe.org/4h1g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4h1g RCSB], [http://www.ebi.ac.uk/pdbsum/4h1g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4h1g ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 15:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4h1g" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Candida albicans wo-1~~]]	+	[[Category: Canaw]]
	[[Category: Allingham, J A]]		[[Category: Allingham, J A]]
	[[Category: Delorme, C]]		[[Category: Delorme, C]]

OCA at 12:56, 5 January 2015

OCA — Mon, 05 Jan 2015 12:56:56 GMT

←Older revision		Revision as of 12:56, 5 January 2015
Line 1:		Line 1:
-	~~{{STRUCTURE_4h1g\| PDB=4h1g \| SCENE= }}~~	+	==Structure of Candida albicans Kar3 motor domain fused to maltose-binding protein==
-	===Structure of Candida albicans Kar3 motor domain fused to maltose-binding protein===	+	<StructureSection load='4h1g' size='340' side='right' caption='[[4h1g]], [[Resolution\|resolution]] 2.15Å' scene=''>
-	~~{{ABSTRACT_PUBMED_23137538}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4h1g]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Candida_albicans_wo-1 Candida albicans wo-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H1G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4H1G FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MTT:MALTOTETRAOSE'>MTT</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3kar\|3kar]], [[3t0q\|3t0q]], [[4gkr\|4gkr]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4h1g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4h1g OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4h1g RCSB], [http://www.ebi.ac.uk/pdbsum/4h1g PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	In the human fungal pathogen Candida albicans, the Kinesin-14 motor protein Kar3 (CaKar3) is critical for normal mitotic division, nuclear fusion during mating, and morphogenic transition from the commensal yeast form to the virulent hyphal form. As a first step towards detailed characterization of this motor of potential medical significance, we have crystallized and determined the X-ray structure of the motor domain of CaKar3 as a maltose-binding protein (MBP) fusion. The structure shows strong conservation of overall motor domain topology to other Kar3 kinesins, but with some prominent differences in one of the motifs that compose the nucleotide-binding pocket and the surface charge distribution. The MBP and Kar3 modules are arranged such that MBP interacts with the Kar3 motor domain core at the same site where the neck linker of conventional kinesins docks during the "ATP state" of the mechanochemical cycle. This site differs from the Kar3 neck-core interface in the recent structure of the ScKar3Vik1 heterodimer. The position of MBP is also completely distinct from the Vik1 subunit in this complex. This may suggest that the site of MBP interaction on the CaKar3 motor domain provides an interface for the neck, or perhaps a partner subunit, at an intermediate state of its motile cycle that has not yet been observed for Kinesin-14 motors.

-	~~==About this Structure==~~	+	Crystal structure of the Candida albicans Kar3 kinesin motor domain fused to maltose-binding protein.,Delorme C, Joshi M, Allingham JS Biochem Biophys Res Commun. 2012 Nov 30;428(4):427-32. doi:, 10.1016/j.bbrc.2012.10.101. Epub 2012 Nov 5. PMID:23137538<ref>PMID:23137538</ref>
-	~~[[4h1g]] is a 1 chain~~ structure ~~with sequence from [http~~:~~//en~~.~~wikipedia~~.~~org~~/~~wiki/Candida_albicans_wo-1 Candida albicans wo-1]~~. ~~Full crystallographic information is available from [http~~://~~oca~~.~~weizmann~~.ac.il/~~oca-bin/ocashort?id~~=~~4H1G OCA].~~	+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Candida albicans wo-1]]		[[Category: Candida albicans wo-1]]
-	[[Category: Allingham, J A.]]	+	[[Category: Allingham, J A]]
-	[[Category: Delorme, C.]]	+	[[Category: Delorme, C]]
-	[[Category: Duan, D.]]	+	[[Category: Duan, D]]
-	[[Category: Joshi, M.]]	+	[[Category: Joshi, M]]
	[[Category: Chimera]]		[[Category: Chimera]]
	[[Category: Kinesin motor domain]]		[[Category: Kinesin motor domain]]
	[[Category: Motor protein]]		[[Category: Motor protein]]

OCA at 06:51, 8 May 2013

OCA — Wed, 08 May 2013 06:51:16 GMT

←Older revision		Revision as of 06:51, 8 May 2013
Line 1:		Line 1:
-	[[Image:4h1g.jpg\|left\|200px]]
-
	{{STRUCTURE_4h1g\| PDB=4h1g \| SCENE= }}		{{STRUCTURE_4h1g\| PDB=4h1g \| SCENE= }}
-
	===Structure of Candida albicans Kar3 motor domain fused to maltose-binding protein===		===Structure of Candida albicans Kar3 motor domain fused to maltose-binding protein===
-		+	{{ABSTRACT_PUBMED_23137538}}

	==About this Structure==		==About this Structure==
-	[[4h1g]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H1G OCA].	+	[[4h1g]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Candida_albicans_wo-1 Candida albicans wo-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H1G OCA].
		+	[[Category: Candida albicans wo-1]]
	[[Category: Allingham, J A.]]		[[Category: Allingham, J A.]]
	[[Category: Delorme, C.]]		[[Category: Delorme, C.]]

OCA at 07:29, 10 October 2012

OCA — Wed, 10 Oct 2012 07:29:48 GMT

←Older revision		Revision as of 07:29, 10 October 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:4h1g.jpg\|left\|200px]]

-	~~The entry~~ 4h1g ~~is ON HOLD~~	+	{{STRUCTURE_4h1g\| PDB=4h1g \| SCENE= }}

-	~~Authors: Allingham, J.A., Duan, D., Delorme, C., Joshi, M.~~	+	===Structure of Candida albicans Kar3 motor domain fused to maltose-binding protein===

-	~~Description:~~ Structure ~~of Candida albicans Kar3~~ motor domain ~~fused to maltose-binding~~ protein	+
		+	==About this Structure==
		+	[[4h1g]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H1G OCA].
		+	[[Category: Allingham, J A.]]
		+	[[Category: Delorme, C.]]
		+	[[Category: Duan, D.]]
		+	[[Category: Joshi, M.]]
		+	[[Category: Chimera]]
		+	[[Category: Kinesin motor domain]]
		+	[[Category: Motor protein]]

OCA: Protected "4h1g" [edit=sysop:move=sysop]

OCA — Wed, 19 Sep 2012 06:57:28 GMT

Protected "4h1g" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:57, 19 September 2012

OCA: New page: '''Unreleased structure''' The entry 4h1g is ON HOLD Authors: Allingham, J.A., Duan, D., Delorme, C., Joshi, M. Description: Structure of Candida albicans Kar3 motor domain fused to ma...

OCA — Wed, 19 Sep 2012 06:57:27 GMT

New page: '''Unreleased structure''' The entry 4h1g is ON HOLD Authors: Allingham, J.A., Duan, D., Delorme, C., Joshi, M. Description: Structure of Candida albicans Kar3 motor domain fused to ma...

New page

'''Unreleased structure'''

The entry 4h1g is ON HOLD

Authors: Allingham, J.A., Duan, D., Delorme, C., Joshi, M.

Description: Structure of Candida albicans Kar3 motor domain fused to maltose-binding protein