4h2x - Revision history

OCA at 07:56, 3 November 2022

OCA — Thu, 03 Nov 2022 07:56:44 GMT

←Older revision		Revision as of 07:56, 3 November 2022
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	==Crystal structure of engineered Bradyrhizobium japonicum glycine:[carrier protein] ligase complexed with carrier protein from Agrobacterium tumefaciens and an analogue of glycyl adenylate==		==Crystal structure of engineered Bradyrhizobium japonicum glycine:[carrier protein] ligase complexed with carrier protein from Agrobacterium tumefaciens and an analogue of glycyl adenylate==
-	<StructureSection load='4h2x' size='340' side='right' caption='[[4h2x]], [[Resolution\|resolution]] 2.15Å' scene=''>	+	<StructureSection load='4h2x' size='340' side='right'caption='[[4h2x]], [[Resolution\|resolution]] 2.15Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4h2x]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Agrfc Agrfc~~] and [~~http~~://en.wikipedia.org/wiki/~~Bradu Bradu~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H2X OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4H2X FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4h2x]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Agrobacterium_fabrum_str._C58 Agrobacterium fabrum str. C58] and [https://en.wikipedia.org/wiki/Bradyrhizobium_diazoefficiens_USDA_110 Bradyrhizobium diazoefficiens USDA 110]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H2X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4H2X FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=G5A:5-O-(GLYCYLSULFAMOYL)ADENOSINE'>G5A</scene>, <scene name='pdbligand=PNS:4-PHOSPHOPANTETHEINE'>PNS</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene>, <scene name='pdbligand=G5A:5-O-(GLYCYLSULFAMOYL)ADENOSINE'>G5A</scene>, <scene name='pdbligand=PNS:4-PHOSPHOPANTETHEINE'>PNS</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	~~<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene></td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4h2x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4h2x OCA], [https://pdbe.org/4h2x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4h2x RCSB], [https://www.ebi.ac.uk/pdbsum/4h2x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4h2x ProSAT]</span></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3mf2\|3mf2]], [[4h2s\|4h2s]], [[4h2t\|4h2t]], [[4h2u\|4h2u]], [[4h2v\|4h2v]], [[4h2w\|4h2w]], [[4h2y\|4h2y]]</td></tr>	+
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Atu2573,AGR_C_4663 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224911 BRADU]), AGR_C_4658, Atu2571 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=176299 AGRFC])</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4h2x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4h2x OCA], [~~http~~://pdbe.org/4h2x PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4h2x RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4h2x PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4h2x ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/~~AACL1_BRAJA AACL1_BRAJA]~~] Catalyzes the ATP-dependent activation of L-glycine and its transfer to the phosphopantetheine prosthetic group covalently attached to the vicinal carrier protein bsr0959 of yet unknown function. May participate in nonribosomal peptide synthesis or related processes. L-alanine is a poor substrate whereas L-serine or D-amino acids are not substrates for ATP-dependent activation. Does not display tRNA aminoacylation activity. ~~[[http~~://www.uniprot.org/uniprot/AACP_AGRT5 AACP_AGRT5]] Aminoacyl carrier protein. Can be charged with L-alanine, L-glycine or L-serine, via the formation of a thioester bond between the amino acid and the 4'-phosphopantetheinyl prosthetic group, catalyzed by the Atu2573 ligase.	+	[https://www.uniprot.org/uniprot/AACL1_BRADU AACL1_BRADU] Catalyzes the ATP-dependent activation of L-glycine and its transfer to the phosphopantetheine prosthetic group covalently attached to the vicinal carrier protein bsr0959 of yet unknown function. May participate in nonribosomal peptide synthesis or related processes. L-alanine is a poor substrate whereas L-serine or D-amino acids are not substrates for ATP-dependent activation. Does not display tRNA aminoacylation activity.<ref>PMID:20663952</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 25:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Agrfc~~]]	+	[[Category: Agrobacterium fabrum str. C58]]
-	[[Category: ~~Bradu~~]]	+	[[Category: Bradyrhizobium diazoefficiens USDA 110]]
-	[[Category: Ivic, N]]	+	[[Category: Large Structures]]
-	[[Category: Luic, M]]	+	[[Category: Ivic N]]
-	[[Category: Mocibob, M]]	+	[[Category: Luic M]]
-	[[Category: Weygand-Durasevic, I]]	+	[[Category: Mocibob M]]
-	~~[[Category: Aminoacyl-trna synthetase]]~~	+	[[Category: Weygand-Durasevic I]]
-	~~[[Category: Atp binding]]~~	+
-	~~[[Category: Carrier protein]]~~	+
-	~~[[Category: Glycine binding]]~~	+
-	~~[[Category: Ligase]]~~	+
-	~~[[Category: Seryl-trna synthetase~~]]	+

OCA at 22:28, 15 November 2017

OCA — Wed, 15 Nov 2017 22:28:43 GMT

←Older revision		Revision as of 22:28, 15 November 2017
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	<StructureSection load='4h2x' size='340' side='right' caption='[[4h2x]], [[Resolution\|resolution]] 2.15Å' scene=''>		<StructureSection load='4h2x' size='340' side='right' caption='[[4h2x]], [[Resolution\|resolution]] 2.15Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4h2x]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Agrt5 Agrt5~~] and [http://en.wikipedia.org/wiki/Bradu Bradu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H2X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4H2X FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4h2x]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Agrfc Agrfc] and [http://en.wikipedia.org/wiki/Bradu Bradu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H2X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4H2X FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=G5A:5-O-(GLYCYLSULFAMOYL)ADENOSINE'>G5A</scene>, <scene name='pdbligand=PNS:4-PHOSPHOPANTETHEINE'>PNS</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=G5A:5-O-(GLYCYLSULFAMOYL)ADENOSINE'>G5A</scene>, <scene name='pdbligand=PNS:4-PHOSPHOPANTETHEINE'>PNS</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3mf2\|3mf2]], [[4h2s\|4h2s]], [[4h2t\|4h2t]], [[4h2u\|4h2u]], [[4h2v\|4h2v]], [[4h2w\|4h2w]], [[4h2y\|4h2y]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3mf2\|3mf2]], [[4h2s\|4h2s]], [[4h2t\|4h2t]], [[4h2u\|4h2u]], [[4h2v\|4h2v]], [[4h2w\|4h2w]], [[4h2y\|4h2y]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">~~bll0957~~ ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224911 BRADU]), AGR_C_4658, Atu2571 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=176299 ~~AGRT5~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Atu2573,AGR_C_4663 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224911 BRADU]), AGR_C_4658, Atu2571 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=176299 AGRFC])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4h2x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4h2x OCA], [http://pdbe.org/4h2x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4h2x RCSB], [http://www.ebi.ac.uk/pdbsum/4h2x PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4h2x ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4h2x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4h2x OCA], [http://pdbe.org/4h2x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4h2x RCSB], [http://www.ebi.ac.uk/pdbsum/4h2x PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4h2x ProSAT]</span></td></tr>
	</table>		</table>
Line 25:		Line 25:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Agrt5~~]]	+	[[Category: Agrfc]]
	[[Category: Bradu]]		[[Category: Bradu]]
	[[Category: Ivic, N]]		[[Category: Ivic, N]]

OCA at 08:05, 4 August 2016

OCA — Thu, 04 Aug 2016 08:05:02 GMT

←Older revision		Revision as of 08:05, 4 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of engineered Bradyrhizobium japonicum glycine:[carrier protein] ligase complexed with carrier protein from Agrobacterium tumefaciens and an analogue of glycyl adenylate==		==Crystal structure of engineered Bradyrhizobium japonicum glycine:[carrier protein] ligase complexed with carrier protein from Agrobacterium tumefaciens and an analogue of glycyl adenylate==
	<StructureSection load='4h2x' size='340' side='right' caption='[[4h2x]], [[Resolution\|resolution]] 2.15Å' scene=''>		<StructureSection load='4h2x' size='340' side='right' caption='[[4h2x]], [[Resolution\|resolution]] 2.15Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4h2x]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Agrobacterium_fabrum_str._c58 Agrobacterium fabrum str. c58~~] and [http://en.wikipedia.org/wiki/~~Bradyrhizobium_japonicum_usda_110 Bradyrhizobium japonicum usda 110~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H2X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4H2X FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4h2x]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Agrt5 Agrt5] and [http://en.wikipedia.org/wiki/Bradu Bradu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H2X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4H2X FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=G5A:5-O-(GLYCYLSULFAMOYL)ADENOSINE'>G5A</scene>, <scene name='pdbligand=PNS:4-PHOSPHOPANTETHEINE'>PNS</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=G5A:5-O-(GLYCYLSULFAMOYL)ADENOSINE'>G5A</scene>, <scene name='pdbligand=PNS:4-PHOSPHOPANTETHEINE'>PNS</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3mf2\|3mf2]], [[4h2s\|4h2s]], [[4h2t\|4h2t]], [[4h2u\|4h2u]], [[4h2v\|4h2v]], [[4h2w\|4h2w]], [[4h2y\|4h2y]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3mf2\|3mf2]], [[4h2s\|4h2s]], [[4h2t\|4h2t]], [[4h2u\|4h2u]], [[4h2v\|4h2v]], [[4h2w\|4h2w]], [[4h2y\|4h2y]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bll0957 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224911 ~~Bradyrhizobium japonicum USDA 110~~]), AGR_C_4658, Atu2571 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=176299 ~~Agrobacterium fabrum str. C58~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bll0957 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224911 BRADU]), AGR_C_4658, Atu2571 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=176299 AGRT5])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4h2x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4h2x OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4h2x RCSB], [http://www.ebi.ac.uk/pdbsum/4h2x PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4h2x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4h2x OCA], [http://pdbe.org/4h2x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4h2x RCSB], [http://www.ebi.ac.uk/pdbsum/4h2x PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4h2x ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 19:		Line 20:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4h2x" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Agrobacterium fabrum str. c58~~]]	+	[[Category: Agrt5]]
-	[[Category: ~~Bradyrhizobium japonicum usda 110~~]]	+	[[Category: Bradu]]
	[[Category: Ivic, N]]		[[Category: Ivic, N]]
	[[Category: Luic, M]]		[[Category: Luic, M]]

OCA at 07:11, 25 December 2014

OCA — Thu, 25 Dec 2014 07:11:00 GMT

←Older revision		Revision as of 07:11, 25 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_4h2x\| PDB=4h2x \| SCENE= }}~~	+	==Crystal structure of engineered Bradyrhizobium japonicum glycine:[carrier protein] ligase complexed with carrier protein from Agrobacterium tumefaciens and an analogue of glycyl adenylate==
-	===Crystal structure of engineered Bradyrhizobium japonicum glycine:[carrier protein] ligase complexed with carrier protein from Agrobacterium tumefaciens and an analogue of glycyl adenylate===	+	<StructureSection load='4h2x' size='340' side='right' caption='[[4h2x]], [[Resolution\|resolution]] 2.15Å' scene=''>
-	~~{{ABSTRACT_PUBMED_23541895}}~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[4h2x]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Agrobacterium_fabrum_str._c58 Agrobacterium fabrum str. c58] and [http://en.wikipedia.org/wiki/Bradyrhizobium_japonicum_usda_110 Bradyrhizobium japonicum usda 110]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H2X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4H2X FirstGlance]. <br>
-	==Function==	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=G5A:5-O-(GLYCYLSULFAMOYL)ADENOSINE'>G5A</scene>, <scene name='pdbligand=PNS:4-PHOSPHOPANTETHEINE'>PNS</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3mf2\|3mf2]], [[4h2s\|4h2s]], [[4h2t\|4h2t]], [[4h2u\|4h2u]], [[4h2v\|4h2v]], [[4h2w\|4h2w]], [[4h2y\|4h2y]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bll0957 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224911 Bradyrhizobium japonicum USDA 110]), AGR_C_4658, Atu2571 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=176299 Agrobacterium fabrum str. C58])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4h2x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4h2x OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4h2x RCSB], [http://www.ebi.ac.uk/pdbsum/4h2x PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
	[[http://www.uniprot.org/uniprot/AACL1_BRAJA AACL1_BRAJA]] Catalyzes the ATP-dependent activation of L-glycine and its transfer to the phosphopantetheine prosthetic group covalently attached to the vicinal carrier protein bsr0959 of yet unknown function. May participate in nonribosomal peptide synthesis or related processes. L-alanine is a poor substrate whereas L-serine or D-amino acids are not substrates for ATP-dependent activation. Does not display tRNA aminoacylation activity. [[http://www.uniprot.org/uniprot/AACP_AGRT5 AACP_AGRT5]] Aminoacyl carrier protein. Can be charged with L-alanine, L-glycine or L-serine, via the formation of a thioester bond between the amino acid and the 4'-phosphopantetheinyl prosthetic group, catalyzed by the Atu2573 ligase.		[[http://www.uniprot.org/uniprot/AACL1_BRAJA AACL1_BRAJA]] Catalyzes the ATP-dependent activation of L-glycine and its transfer to the phosphopantetheine prosthetic group covalently attached to the vicinal carrier protein bsr0959 of yet unknown function. May participate in nonribosomal peptide synthesis or related processes. L-alanine is a poor substrate whereas L-serine or D-amino acids are not substrates for ATP-dependent activation. Does not display tRNA aminoacylation activity. [[http://www.uniprot.org/uniprot/AACP_AGRT5 AACP_AGRT5]] Aminoacyl carrier protein. Can be charged with L-alanine, L-glycine or L-serine, via the formation of a thioester bond between the amino acid and the 4'-phosphopantetheinyl prosthetic group, catalyzed by the Atu2573 ligase.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Amino acid:[carrier protein] ligases (aa:CP ligases) are recently discovered enzymes that are highly similar to class II aminoacyl-tRNA synthetases (aaRSs). However, while aaRSs aminoacylate tRNA and supply building blocks for ribosomal translation, aa:CP ligases transfer activated amino acids to the phosphopantetheine group of small carrier proteins. We have solved the crystal structure of an aa:CP ligase complexed with the carrier protein (CP). The CP prosthetic group enters the active site from a different direction than tRNA in class II aaRS complexes through an idiosyncratic tunnel. CP binds to aa:CP ligase in a fundamentally different manner compared to tRNA binding by structurally closely related aaRSs. Based on crystallographic analysis, an enzyme of altered CP specificity was designed, and the mechanism of amino acid transfer to the prosthetic group was proposed. The presented study reveals how a conserved class II aaRS catalytic core can adapt to another function through minor structural alterations.

-	~~==About this~~ Structure==	+	Adaptation of Aminoacyl-tRNA Synthetase Catalytic Core to Carrier Protein Aminoacylation.,Mocibob M, Ivic N, Luic M, Weygand-Durasevic I Structure. 2013 Mar 26. pii: S0969-2126(13)00058-0. doi:, 10.1016/j.str.2013.02.017. PMID:23541895<ref>PMID:23541895</ref>
-	~~[[4h2x]] is a 4 chain structure with sequence from [http~~:~~//en~~.~~wikipedia~~.~~org~~/~~wiki/Agrobacterium_fabrum_str~~.~~_c58 Agrobacterium fabrum~~ str. ~~c58] and [http://en~~.~~wikipedia~~.~~org/wiki/Bradyrhizobium_japonicum_usda_110 Bradyrhizobium japonicum usda 110]~~. ~~Full crystallographic information is available from [http~~:/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=4H2X OCA].~~	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group="xtra">PMID:023541895</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Agrobacterium fabrum str. c58]]		[[Category: Agrobacterium fabrum str. c58]]
	[[Category: Bradyrhizobium japonicum usda 110]]		[[Category: Bradyrhizobium japonicum usda 110]]
-	[[Category: Ivic, N.]]	+	[[Category: Ivic, N]]
-	[[Category: Luic, M.]]	+	[[Category: Luic, M]]
-	[[Category: Mocibob, M.]]	+	[[Category: Mocibob, M]]
-	[[Category: Weygand-Durasevic, I.]]	+	[[Category: Weygand-Durasevic, I]]
	[[Category: Aminoacyl-trna synthetase]]		[[Category: Aminoacyl-trna synthetase]]
	[[Category: Atp binding]]		[[Category: Atp binding]]

OCA at 08:43, 19 June 2013

OCA — Wed, 19 Jun 2013 08:43:53 GMT

←Older revision		Revision as of 08:43, 19 June 2013
Line 10:		Line 10:

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~020663952~~</ref><references group="xtra"/><references/>	+	<ref group="xtra">PMID:023541895</ref><references group="xtra"/><references/>
	[[Category: Agrobacterium fabrum str. c58]]		[[Category: Agrobacterium fabrum str. c58]]
	[[Category: Bradyrhizobium japonicum usda 110]]		[[Category: Bradyrhizobium japonicum usda 110]]

OCA at 21:56, 10 April 2013

OCA — Wed, 10 Apr 2013 21:56:22 GMT

←Older revision		Revision as of 21:56, 10 April 2013
Line 1:		Line 1:
-	[[Image:4h2x.jpg\|left\|200px]]
-
	{{STRUCTURE_4h2x\| PDB=4h2x \| SCENE= }}		{{STRUCTURE_4h2x\| PDB=4h2x \| SCENE= }}
-
	===Crystal structure of engineered Bradyrhizobium japonicum glycine:[carrier protein] ligase complexed with carrier protein from Agrobacterium tumefaciens and an analogue of glycyl adenylate===		===Crystal structure of engineered Bradyrhizobium japonicum glycine:[carrier protein] ligase complexed with carrier protein from Agrobacterium tumefaciens and an analogue of glycyl adenylate===
		+	{{ABSTRACT_PUBMED_23541895}}

-	~~{{ABSTRACT_PUBMED_20663952}}~~	+	==Function==
		+	[[http://www.uniprot.org/uniprot/AACL1_BRAJA AACL1_BRAJA]] Catalyzes the ATP-dependent activation of L-glycine and its transfer to the phosphopantetheine prosthetic group covalently attached to the vicinal carrier protein bsr0959 of yet unknown function. May participate in nonribosomal peptide synthesis or related processes. L-alanine is a poor substrate whereas L-serine or D-amino acids are not substrates for ATP-dependent activation. Does not display tRNA aminoacylation activity. [[http://www.uniprot.org/uniprot/AACP_AGRT5 AACP_AGRT5]] Aminoacyl carrier protein. Can be charged with L-alanine, L-glycine or L-serine, via the formation of a thioester bond between the amino acid and the 4'-phosphopantetheinyl prosthetic group, catalyzed by the Atu2573 ligase.

	==About this Structure==		==About this Structure==
Line 11:		Line 10:

	==Reference==		==Reference==
-	<ref group="xtra">PMID:020663952</ref><references group="xtra"/>	+	<ref group="xtra">PMID:020663952</ref><references group="xtra"/><references/>
	[[Category: Agrobacterium fabrum str. c58]]		[[Category: Agrobacterium fabrum str. c58]]
	[[Category: Bradyrhizobium japonicum usda 110]]		[[Category: Bradyrhizobium japonicum usda 110]]

OCA at 14:27, 6 March 2013

OCA — Wed, 06 Mar 2013 14:27:27 GMT

←Older revision		Revision as of 14:27, 6 March 2013
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:4h2x.jpg\|left\|200px]]

-	~~The entry~~ 4h2x ~~is ON HOLD~~ ~~until Paper Publication~~	+	{{STRUCTURE_4h2x\| PDB=4h2x \| SCENE= }}

-	~~Authors~~: ~~Luic, M., Weygand-Durasevic, I., Ivic, N., Mocibob, M.~~	+	===Crystal structure of engineered Bradyrhizobium japonicum glycine:[carrier protein] ligase complexed with carrier protein from Agrobacterium tumefaciens and an analogue of glycyl adenylate===

-	~~Description: Crystal~~ structure ~~of engineered~~ Bradyrhizobium japonicum ~~glycine~~:[~~carrier protein~~] ~~ligase complexed with carrier~~ protein ~~from Agrobacterium tumefaciens and an analogue of glycyl adenylate~~	+	{{ABSTRACT_PUBMED_20663952}}
		+
		+	==About this Structure==
		+	[[4h2x]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Agrobacterium_fabrum_str._c58 Agrobacterium fabrum str. c58] and [http://en.wikipedia.org/wiki/Bradyrhizobium_japonicum_usda_110 Bradyrhizobium japonicum usda 110]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H2X OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:020663952</ref><references group="xtra"/>
		+	[[Category: Agrobacterium fabrum str. c58]]
		+	[[Category: Bradyrhizobium japonicum usda 110]]
		+	[[Category: Ivic, N.]]
		+	[[Category: Luic, M.]]
		+	[[Category: Mocibob, M.]]
		+	[[Category: Weygand-Durasevic, I.]]
		+	[[Category: Aminoacyl-trna synthetase]]
		+	[[Category: Atp binding]]
		+	[[Category: Carrier protein]]
		+	[[Category: Glycine binding]]
		+	[[Category: Ligase]]
		+	[[Category: Seryl-trna synthetase]]

OCA at 05:47, 3 October 2012

OCA — Wed, 03 Oct 2012 05:47:07 GMT

←Older revision		Revision as of 05:47, 3 October 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4h2x is ON HOLD	+	The entry 4h2x is ON HOLD until Paper Publication

	Authors: Luic, M., Weygand-Durasevic, I., Ivic, N., Mocibob, M.		Authors: Luic, M., Weygand-Durasevic, I., Ivic, N., Mocibob, M.

	Description: Crystal structure of engineered Bradyrhizobium japonicum glycine:[carrier protein] ligase complexed with carrier protein from Agrobacterium tumefaciens and an analogue of glycyl adenylate		Description: Crystal structure of engineered Bradyrhizobium japonicum glycine:[carrier protein] ligase complexed with carrier protein from Agrobacterium tumefaciens and an analogue of glycyl adenylate

OCA: Protected "4h2x" [edit=sysop:move=sysop]

OCA — Wed, 19 Sep 2012 06:58:58 GMT

Protected "4h2x" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:58, 19 September 2012

OCA: New page: '''Unreleased structure''' The entry 4h2x is ON HOLD Authors: Luic, M., Weygand-Durasevic, I., Ivic, N., Mocibob, M. Description: Crystal structure of engineered Bradyrhizobium japonic...

OCA — Wed, 19 Sep 2012 06:58:57 GMT

New page: '''Unreleased structure''' The entry 4h2x is ON HOLD Authors: Luic, M., Weygand-Durasevic, I., Ivic, N., Mocibob, M. Description: Crystal structure of engineered Bradyrhizobium japonic...

New page

'''Unreleased structure'''

The entry 4h2x is ON HOLD

Authors: Luic, M., Weygand-Durasevic, I., Ivic, N., Mocibob, M.

Description: Crystal structure of engineered Bradyrhizobium japonicum glycine:[carrier protein] ligase complexed with carrier protein from Agrobacterium tumefaciens and an analogue of glycyl adenylate