4h3h - Revision history

OCA at 07:57, 3 November 2022

OCA — Thu, 03 Nov 2022 07:57:38 GMT

←Older revision		Revision as of 07:57, 3 November 2022
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	==Crystal structure of a ternary complex of human symplekin NTD, human Ssu72 and a RNA poymerase II CTD peptide phosphorylated at SER-7==		==Crystal structure of a ternary complex of human symplekin NTD, human Ssu72 and a RNA poymerase II CTD peptide phosphorylated at SER-7==
-	<StructureSection load='4h3h' size='340' side='right' caption='[[4h3h]], [[Resolution\|resolution]] 2.20Å' scene=''>	+	<StructureSection load='4h3h' size='340' side='right'caption='[[4h3h]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4h3h]] is a 5 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H3H OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4H3H FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4h3h]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H3H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4H3H FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene></td></tr>
-	~~<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat">~~<scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene~~></td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4h3h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4h3h OCA], [https://pdbe.org/4h3h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4h3h RCSB], [https://www.ebi.ac.uk/pdbsum/4h3h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4h3h ProSAT]</span></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3o2q\|3o2q]], [[4h3k\|4h3k]]</td></tr>~~	+
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SYMPK, SPK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), SSU72, HSPC182, PNAS-120 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphoprotein_phosphatase Phosphoprotein phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.16 3.1.3.16] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4h3h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4h3h OCA], [~~http~~://pdbe.org/4h3h PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4h3h RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4h3h PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4h3h ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/SYMPK_HUMAN SYMPK_HUMAN]] Scaffold protein that functions as a component of a multimolecular complex involved in histone mRNA 3'-end processing. Specific component of the tight junction (TJ) plaque, but might not be an exclusively junctional component. May have a house-keeping rule. Is involved in pre-mRNA polyadenylation. Enhances SSU72 phosphatase activity.<ref>PMID:16230528</ref> <ref>PMID:20861839</ref> [[http://www.uniprot.org/uniprot/SSU72_HUMAN SSU72_HUMAN]] Protein phosphatase that catalyzes the dephosphorylation of the C-terminal domain of RNA polymerase II. Plays a role in RNA processing and termination. Plays a role in pre-mRNA polyadenylation via its interaction with SYMPK.<ref>PMID:15659578</ref> <ref>PMID:20861839</ref> <ref>PMID:23070812</ref>	+	[https://www.uniprot.org/uniprot/SYMPK_HUMAN SYMPK_HUMAN] Scaffold protein that functions as a component of a multimolecular complex involved in histone mRNA 3'-end processing. Specific component of the tight junction (TJ) plaque, but might not be an exclusively junctional component. May have a house-keeping rule. Is involved in pre-mRNA polyadenylation. Enhances SSU72 phosphatase activity.<ref>PMID:16230528</ref> <ref>PMID:20861839</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 4h3h" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4h3h" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Symplekin\|Symplekin]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
-	[[Category: ~~Phosphoprotein phosphatase~~]]	+	[[Category: Large Structures]]
-	[[Category: Tong, L]]	+	[[Category: Tong L]]
-	[[Category: Xiang, K]]	+	[[Category: Xiang K]]
-	~~[[Category: Heat repeat]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Phosphatase]]~~	+
-	~~[[Category: Rna polymerase ii~~]]	+

OCA at 08:35, 15 November 2017

OCA — Wed, 15 Nov 2017 08:35:26 GMT

←Older revision		Revision as of 08:35, 15 November 2017
Line 22:		Line 22:
	</div>		</div>
	<div class="pdbe-citations 4h3h" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4h3h" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Symplekin\|Symplekin]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 22:13, 4 August 2016

OCA — Thu, 04 Aug 2016 22:13:24 GMT

←Older revision		Revision as of 22:13, 4 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of a ternary complex of human symplekin NTD, human Ssu72 and a RNA poymerase II CTD peptide phosphorylated at SER-7==		==Crystal structure of a ternary complex of human symplekin NTD, human Ssu72 and a RNA poymerase II CTD peptide phosphorylated at SER-7==
	<StructureSection load='4h3h' size='340' side='right' caption='[[4h3h]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='4h3h' size='340' side='right' caption='[[4h3h]], [[Resolution\|resolution]] 2.20Å' scene=''>
Line 8:		Line 9:
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SYMPK, SPK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), SSU72, HSPC182, PNAS-120 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SYMPK, SPK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), SSU72, HSPC182, PNAS-120 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphoprotein_phosphatase Phosphoprotein phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.16 3.1.3.16] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphoprotein_phosphatase Phosphoprotein phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.16 3.1.3.16] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4h3h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4h3h OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4h3h RCSB], [http://www.ebi.ac.uk/pdbsum/4h3h PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4h3h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4h3h OCA], [http://pdbe.org/4h3h PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4h3h RCSB], [http://www.ebi.ac.uk/pdbsum/4h3h PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4h3h ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 20:		Line 21:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4h3h" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Symplekin\|Symplekin]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 13:34, 25 December 2014

OCA — Thu, 25 Dec 2014 13:34:55 GMT

←Older revision		Revision as of 13:34, 25 December 2014
Line 10:		Line 10:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4h3h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4h3h OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4h3h RCSB], [http://www.ebi.ac.uk/pdbsum/4h3h PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4h3h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4h3h OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4h3h RCSB], [http://www.ebi.ac.uk/pdbsum/4h3h PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/SYMPK_HUMAN SYMPK_HUMAN]] Scaffold protein that functions as a component of a multimolecular complex involved in histone mRNA 3'-end processing. Specific component of the tight junction (TJ) plaque, but might not be an exclusively junctional component. May have a house-keeping rule. Is involved in pre-mRNA polyadenylation. Enhances SSU72 phosphatase activity.<ref>PMID:16230528</ref> <ref>PMID:20861839</ref> [[http://www.uniprot.org/uniprot/SSU72_HUMAN SSU72_HUMAN]] Protein phosphatase that catalyzes the dephosphorylation of the C-terminal domain of RNA polymerase II. Plays a role in RNA processing and termination. Plays a role in pre-mRNA polyadenylation via its interaction with SYMPK.<ref>PMID:15659578</ref> <ref>PMID:20861839</ref> <ref>PMID:23070812</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 09:33, 10 December 2014

OCA — Wed, 10 Dec 2014 09:33:04 GMT

←Older revision		Revision as of 09:33, 10 December 2014
Line 1:		Line 1:
-	[[~~Image:~~4h3h.~~jpg~~\|~~left~~\|~~200px~~]]	+	==Crystal structure of a ternary complex of human symplekin NTD, human Ssu72 and a RNA poymerase II CTD peptide phosphorylated at SER-7==
		+	<StructureSection load='4h3h' size='340' side='right' caption='[[4h3h]], [[Resolution\|resolution]] 2.20Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4h3h]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H3H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4H3H FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3o2q\|3o2q]], [[4h3k\|4h3k]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SYMPK, SPK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), SSU72, HSPC182, PNAS-120 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphoprotein_phosphatase Phosphoprotein phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.16 3.1.3.16] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4h3h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4h3h OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4h3h RCSB], [http://www.ebi.ac.uk/pdbsum/4h3h PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Ssu72, an RNA polymerase II C-terminal domain (CTD) phospho-Ser5 (pSer5) phosphatase, was recently reported to have pSer7 phosphatase activity as well. We report here the crystal structure of a ternary complex of the N-terminal domain of human symplekin, human Ssu72, and a 10-mer pSer7 CTD peptide. Surprisingly, the peptide is bound in the Ssu72 active site with its backbone running in the opposite direction compared with a pSer5 peptide. The pSer7 phosphatase activity of Ssu72 is approximately 4000-fold lower than its pSer5 phosphatase activity toward a peptide substrate, consistent with the structural observations.

-	~~{{STRUCTURE_4h3h\| PDB=4h3h \| SCENE= }}~~	+	An unexpected binding mode for a Pol II CTD peptide phosphorylated at Ser7 in the active site of the CTD phosphatase Ssu72.,Xiang K, Manley JL, Tong L Genes Dev. 2012 Oct 15;26(20):2265-70. doi: 10.1101/gad.198853.112. PMID:23070812<ref>PMID:23070812</ref>

-	~~===Crystal structure of~~ a ~~ternary complex~~ of ~~human symplekin NTD, human Ssu72 and a RNA poymerase II CTD peptide phosphorylated at SER-7===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_23070812}}~~	+	== References ==
-		+	<references/>
-	==~~About this Structure~~==	+	__TOC__
-	~~[[4h3h]] is a 5 chain structure with sequence from [http:~~//~~en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H3H OCA].~~	+	</StructureSection>
-	[[Category: ~~Homo sapiens~~]]	+	[[Category: Human]]
	[[Category: Phosphoprotein phosphatase]]		[[Category: Phosphoprotein phosphatase]]
-	[[Category: Tong, L.]]	+	[[Category: Tong, L]]
-	[[Category: Xiang, K.]]	+	[[Category: Xiang, K]]
	[[Category: Heat repeat]]		[[Category: Heat repeat]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Phosphatase]]		[[Category: Phosphatase]]
	[[Category: Rna polymerase ii]]		[[Category: Rna polymerase ii]]

OCA at 08:58, 31 October 2012

OCA — Wed, 31 Oct 2012 08:58:37 GMT

←Older revision		Revision as of 08:58, 31 October 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:4h3h.jpg\|left\|200px]]

-	~~The entry~~ 4h3h ~~is ON HOLD~~	+	{{STRUCTURE_4h3h\| PDB=4h3h \| SCENE= }}

-	~~Authors: Tong~~, ~~L., Xiang, K.~~	+	===Crystal structure of a ternary complex of human symplekin NTD, human Ssu72 and a RNA poymerase II CTD peptide phosphorylated at SER-7===

-	~~Description: Crystal~~ structure ~~of pSer7 RNA polymerase II CTD phosphopeptide in human symplekin~~-~~Ssu72 complex~~	+	{{ABSTRACT_PUBMED_23070812}}
		+
		+	==About this Structure==
		+	[[4h3h]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H3H OCA].
		+	[[Category: Homo sapiens]]
		+	[[Category: Phosphoprotein phosphatase]]
		+	[[Category: Tong, L.]]
		+	[[Category: Xiang, K.]]
		+	[[Category: Heat repeat]]
		+	[[Category: Hydrolase]]
		+	[[Category: Phosphatase]]
		+	[[Category: Rna polymerase ii]]

OCA: Protected "4h3h" [edit=sysop:move=sysop]

OCA — Wed, 19 Sep 2012 06:59:33 GMT

Protected "4h3h" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:59, 19 September 2012

OCA: New page: '''Unreleased structure''' The entry 4h3h is ON HOLD Authors: Tong, L., Xiang, K. Description: Crystal structure of pSer7 RNA polymerase II CTD phosphopeptide in human symplekin-Ssu72 ...

OCA — Wed, 19 Sep 2012 06:59:32 GMT

New page: '''Unreleased structure''' The entry 4h3h is ON HOLD Authors: Tong, L., Xiang, K. Description: Crystal structure of pSer7 RNA polymerase II CTD phosphopeptide in human symplekin-Ssu72 ...

New page

'''Unreleased structure'''

The entry 4h3h is ON HOLD

Authors: Tong, L., Xiang, K.

Description: Crystal structure of pSer7 RNA polymerase II CTD phosphopeptide in human symplekin-Ssu72 complex