4h3n - Revision history

OCA at 14:59, 20 September 2023

OCA — Wed, 20 Sep 2023 14:59:46 GMT

←Older revision		Revision as of 14:59, 20 September 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4h3n]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Discosoma_sp._LW-2004 Discosoma sp. LW-2004]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H3N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4H3N FirstGlance]. <br>		<table><tr><td colspan='2'>[[4h3n]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Discosoma_sp._LW-2004 Discosoma sp. LW-2004]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H3N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4H3N FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NRQ:{(4Z)-4-(4-HYDROXYBENZYLIDENE)-2-[3-(METHYLTHIO)PROPANIMIDOYL]-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>NRQ</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.75Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NRQ:{(4Z)-4-(4-HYDROXYBENZYLIDENE)-2-[3-(METHYLTHIO)PROPANIMIDOYL]-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>NRQ</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4h3n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4h3n OCA], [https://pdbe.org/4h3n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4h3n RCSB], [https://www.ebi.ac.uk/pdbsum/4h3n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4h3n ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4h3n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4h3n OCA], [https://pdbe.org/4h3n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4h3n RCSB], [https://www.ebi.ac.uk/pdbsum/4h3n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4h3n ProSAT]</span></td></tr>
	</table>		</table>

OCA at 07:57, 3 November 2022

OCA — Thu, 03 Nov 2022 07:57:49 GMT

←Older revision		Revision as of 07:57, 3 November 2022
Line 3:		Line 3:
	<StructureSection load='4h3n' size='340' side='right'caption='[[4h3n]], [[Resolution\|resolution]] 1.75Å' scene=''>		<StructureSection load='4h3n' size='340' side='right'caption='[[4h3n]], [[Resolution\|resolution]] 1.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4h3n]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Discosoma_sp.~~_lw~~-2004 Discosoma sp. lw-2004]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H3N OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4H3N FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4h3n]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Discosoma_sp._LW-2004 Discosoma sp. LW-2004]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H3N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4H3N FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NRQ:{(4Z)-4-(4-HYDROXYBENZYLIDENE)-2-[3-(METHYLTHIO)PROPANIMIDOYL]-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>NRQ</scene></td></tr>
-	~~<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat">~~<scene name='pdbligand=NRQ:{(4Z)-4-(4-HYDROXYBENZYLIDENE)-2-[3-(METHYLTHIO)PROPANIMIDOYL]-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>NRQ</scene>~~</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4h3n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4h3n OCA], [https://pdbe.org/4h3n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4h3n RCSB], [https://www.ebi.ac.uk/pdbsum/4h3n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4h3n ProSAT]</span></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4h3l\|4h3l]], [[4h3m\|4h3m]]~~</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4h3n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4h3n OCA], [~~http~~://pdbe.org/4h3n PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4h3n RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4h3n PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4h3n ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q5S3G7_9CNID Q5S3G7_9CNID]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Discosoma sp. lw-2004]]	+	[[Category: Discosoma sp. LW-2004]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Chica~~, R A~~]]	+	[[Category: Chica RA]]
-	[[Category: Moore~~, M M]]~~	+	[[Category: Moore MM]]
-	~~[[Category: Fluorescent protein~~]]	+

OCA at 09:40, 5 February 2020

OCA — Wed, 05 Feb 2020 09:40:16 GMT

←Older revision		Revision as of 09:40, 5 February 2020
Line 1:		Line 1:

	==mPlumAYC==		==mPlumAYC==
-	<StructureSection load='4h3n' size='340' side='right' caption='[[4h3n]], [[Resolution\|resolution]] 1.75Å' scene=''>	+	<StructureSection load='4h3n' size='340' side='right'caption='[[4h3n]], [[Resolution\|resolution]] 1.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4h3n]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Discosoma_sp._lw-2004 Discosoma sp. lw-2004]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H3N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4H3N FirstGlance]. <br>		<table><tr><td colspan='2'>[[4h3n]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Discosoma_sp._lw-2004 Discosoma sp. lw-2004]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H3N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4H3N FirstGlance]. <br>
Line 23:		Line 23:
	</StructureSection>		</StructureSection>
	[[Category: Discosoma sp. lw-2004]]		[[Category: Discosoma sp. lw-2004]]
		+	[[Category: Large Structures]]
	[[Category: Chica, R A]]		[[Category: Chica, R A]]
	[[Category: Moore, M M]]		[[Category: Moore, M M]]
	[[Category: Fluorescent protein]]		[[Category: Fluorescent protein]]

OCA at 20:08, 15 December 2016

OCA — Thu, 15 Dec 2016 20:08:32 GMT

←Older revision		Revision as of 20:08, 15 December 2016
Line 1:		Line 1:
		+
	==mPlumAYC==		==mPlumAYC==
	<StructureSection load='4h3n' size='340' side='right' caption='[[4h3n]], [[Resolution\|resolution]] 1.75Å' scene=''>		<StructureSection load='4h3n' size='340' side='right' caption='[[4h3n]], [[Resolution\|resolution]] 1.75Å' scene=''>
Line 6:		Line 7:
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NRQ:{(4Z)-4-(4-HYDROXYBENZYLIDENE)-2-[3-(METHYLTHIO)PROPANIMIDOYL]-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>NRQ</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NRQ:{(4Z)-4-(4-HYDROXYBENZYLIDENE)-2-[3-(METHYLTHIO)PROPANIMIDOYL]-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>NRQ</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4h3l\|4h3l]], [[4h3m\|4h3m]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4h3l\|4h3l]], [[4h3m\|4h3m]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4h3n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4h3n OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4h3n RCSB], [http://www.ebi.ac.uk/pdbsum/4h3n PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4h3n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4h3n OCA], [http://pdbe.org/4h3n PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4h3n RCSB], [http://www.ebi.ac.uk/pdbsum/4h3n PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4h3n ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4h3n" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 09:57, 10 December 2014

OCA — Wed, 10 Dec 2014 09:57:37 GMT

←Older revision		Revision as of 09:57, 10 December 2014
Line 1:		Line 1:
-	[[~~Image:~~4h3n.~~png~~\|~~left~~\|~~200px~~]]	+	==mPlumAYC==
		+	<StructureSection load='4h3n' size='340' side='right' caption='[[4h3n]], [[Resolution\|resolution]] 1.75Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4h3n]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Discosoma_sp._lw-2004 Discosoma sp. lw-2004]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H3N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4H3N FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NRQ:{(4Z)-4-(4-HYDROXYBENZYLIDENE)-2-[3-(METHYLTHIO)PROPANIMIDOYL]-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>NRQ</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4h3l\|4h3l]], [[4h3m\|4h3m]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4h3n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4h3n OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4h3n RCSB], [http://www.ebi.ac.uk/pdbsum/4h3n PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Red fluorescent proteins (RFPs) derived from organisms in the class Anthozoa have found widespread application as imaging tools in biological research. For most imaging experiments, RFPs that mature quickly to the red chromophore and produce little or no green chromophore are most useful. In this study, we used rational design to convert a yellow fluorescent mPlum mutant to a red-emitting RFP without reverting any of the mutations causing the maturation deficiency and without altering the red chromophore's covalent structure. We also created an optimized mPlum mutant (mPlum-E16P) that matures almost exclusively to the red chromophore. Analysis of the structure/function relationships in these proteins revealed two structural characteristics that are important for efficient red chromophore maturation in DsRed-derived RFPs. The first is the presence of a lysine residue at position 70 that is able to interact directly with the chromophore. The second is an absence of non-bonding interactions limiting the conformational flexibility at the peptide backbone that is oxidized during red chromophore formation. Satisfying or improving these structural features in other maturation-deficient RFPs may result in RFPs with faster and more complete maturation to the red chromophore.

-	~~{{STRUCTURE_4h3n\| PDB=4h3n \| SCENE= }}~~	+	Recovery of Red Fluorescent Protein Chromophore Maturation Deficiency through Rational Design.,Moore MM, Oteng-Pabi SK, Pandelieva AT, Mayo SL, Chica RA PLoS One. 2012;7(12):e52463. doi: 10.1371/journal.pone.0052463. Epub 2012 Dec 20. PMID:23285050<ref>PMID:23285050</ref>

-	~~===mPlumAYC===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_23285050}}~~	+	== References ==
-		+	<references/>
-	==~~About this Structure~~==	+	__TOC__
-	~~[[4h3n]] is a 1 chain structure with sequence from [http:~~//~~en.wikipedia.org/wiki/Discosoma_sp._lw-2004 Discosoma sp. lw-2004]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H3N OCA].~~	+	</StructureSection>
	[[Category: Discosoma sp. lw-2004]]		[[Category: Discosoma sp. lw-2004]]
-	[[Category: Chica, R A.]]	+	[[Category: Chica, R A]]
-	[[Category: Moore, M M.]]	+	[[Category: Moore, M M]]
	[[Category: Fluorescent protein]]		[[Category: Fluorescent protein]]

OCA at 09:00, 16 January 2013

OCA — Wed, 16 Jan 2013 09:00:52 GMT

←Older revision		Revision as of 09:00, 16 January 2013
Line 1:		Line 1:
-	[[Image:4h3n.~~jpg~~\|left\|200px]]	+	[[Image:4h3n.png\|left\|200px]]

	{{STRUCTURE_4h3n\| PDB=4h3n \| SCENE= }}		{{STRUCTURE_4h3n\| PDB=4h3n \| SCENE= }}
Line 5:		Line 5:
	===mPlumAYC===		===mPlumAYC===

		+	{{ABSTRACT_PUBMED_23285050}}

	==About this Structure==		==About this Structure==

OCA at 07:47, 24 October 2012

OCA — Wed, 24 Oct 2012 07:47:04 GMT

←Older revision		Revision as of 07:47, 24 October 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:4h3n.jpg\|left\|200px]]

-	~~The entry~~ 4h3n ~~is ON HOLD~~	+	{{STRUCTURE_4h3n\| PDB=4h3n \| SCENE= }}

-	~~Authors: Moore, M.M., Chica, R.A.~~	+	===mPlumAYC===

-	~~Description~~: ~~mPlumAYC~~	+
		+	==About this Structure==
		+	[[4h3n]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Discosoma_sp._lw-2004 Discosoma sp. lw-2004]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H3N OCA].
		+	[[Category: Discosoma sp. lw-2004]]
		+	[[Category: Chica, R A.]]
		+	[[Category: Moore, M M.]]
		+	[[Category: Fluorescent protein]]

OCA: Protected "4h3n" [edit=sysop:move=sysop]

OCA — Wed, 19 Sep 2012 06:59:43 GMT

Protected "4h3n" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:59, 19 September 2012

OCA: New page: '''Unreleased structure''' The entry 4h3n is ON HOLD Authors: Moore, M.M., Chica, R.A. Description: mPlumAYC

OCA — Wed, 19 Sep 2012 06:59:42 GMT

New page: '''Unreleased structure''' The entry 4h3n is ON HOLD Authors: Moore, M.M., Chica, R.A. Description: mPlumAYC

New page

'''Unreleased structure'''

The entry 4h3n is ON HOLD

Authors: Moore, M.M., Chica, R.A.

Description: mPlumAYC