4h7a - Revision history

OCA at 15:01, 20 September 2023

OCA — Wed, 20 Sep 2023 15:01:13 GMT

←Older revision		Revision as of 15:01, 20 September 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4h7a]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H7A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4H7A FirstGlance]. <br>		<table><tr><td colspan='2'>[[4h7a]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H7A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4H7A FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4h7a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4h7a OCA], [https://pdbe.org/4h7a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4h7a RCSB], [https://www.ebi.ac.uk/pdbsum/4h7a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4h7a ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.6Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4h7a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4h7a OCA], [https://pdbe.org/4h7a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4h7a RCSB], [https://www.ebi.ac.uk/pdbsum/4h7a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4h7a ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 08:03, 3 November 2022

OCA — Thu, 03 Nov 2022 08:03:34 GMT

←Older revision		Revision as of 08:03, 3 November 2022
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	==Crystal structure of CasB from Thermus thermophilus==		==Crystal structure of CasB from Thermus thermophilus==
-	<StructureSection load='4h7a' size='340' side='right' caption='[[4h7a]], [[Resolution\|resolution]] 2.60Å' scene=''>	+	<StructureSection load='4h7a' size='340' side='right'caption='[[4h7a]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4h7a]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Thet8 Thet8~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H7A OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4H7A FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4h7a]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H7A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4H7A FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cse2, TTHB189 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=300852 THET8])</td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4h7a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4h7a OCA], [https://pdbe.org/4h7a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4h7a RCSB], [https://www.ebi.ac.uk/pdbsum/4h7a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4h7a ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4h7a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4h7a OCA], [~~http~~://pdbe.org/4h7a PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4h7a RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4h7a PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4h7a ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/CSE2_THET8 CSE2_THET8]] CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (By similarity).	+	[https://www.uniprot.org/uniprot/CSE2_THET8 CSE2_THET8] CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 4h7a" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4h7a" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[CRISPR type I-E (Cascade)\|CRISPR type I-E (Cascade)]]
		+	*[[Endonuclease 3D structures\|Endonuclease 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Thet8~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Ke, A~~]]	+	[[Category: Thermus thermophilus HB8]]
-	[[Category: ~~Nam, K H~~]]	+	[[Category: Ke A]]
-	[[Category: ~~Casb]]~~	+	[[Category: Nam KH]]
-	~~[[Category: Cascade]]~~	+
-	~~[[Category: Crispr]]~~	+
-	~~[[Category: Crispr-assoicated protein]]~~	+
-	~~[[Category: Dna binding protein]]~~	+
-	~~[[Category: Nucleic acid binding protein~~]]	+

OCA at 09:45, 25 October 2017

OCA — Wed, 25 Oct 2017 09:45:02 GMT

←Older revision		Revision as of 09:45, 25 October 2017
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	</div>		</div>
	<div class="pdbe-citations 4h7a" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4h7a" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Endonuclease\|Endonuclease]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 14:53, 5 August 2016

OCA — Fri, 05 Aug 2016 14:53:21 GMT

←Older revision

Revision as of 14:53, 5 August 2016

OCA at 19:44, 25 December 2014

OCA — Thu, 25 Dec 2014 19:44:03 GMT

←Older revision		Revision as of 19:44, 25 December 2014
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-	[[~~Image:~~4h7a.~~png~~\|~~left\|200px~~]]	+	==Crystal structure of CasB from Thermus thermophilus==
		+	<StructureSection load='4h7a' size='340' side='right' caption='[[4h7a]], [[Resolution\|resolution]] 2.60Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4h7a]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus_hb8 Thermus thermophilus hb8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H7A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4H7A FirstGlance]. <br>
		+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cse2, TTHB189 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=300852 Thermus thermophilus HB8])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4h7a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4h7a OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4h7a RCSB], [http://www.ebi.ac.uk/pdbsum/4h7a PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/CSE2_THET8 CSE2_THET8]] CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (By similarity).
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The CRISPR system is an adaptive RNA-based microbial immune system against invasive genetic elements. CasB is an essential protein component in Type I-E Cascade. Here, we characterize CasB proteins from three different organisms as non-specific nucleic acid binding proteins. The Thermobifida fusca CasB crystal structure reveals conserved positive surface charges, which we show are important for its nucleic acid binding function. EM docking reveals that CasB dimerization aligns individual nucleic acid binding surfaces into a curved, elongated binding surface inside Type I-E Cascade, consistent with the putative functions of CasB in ds-DNA recruitment and crRNA-DNA duplex formation steps. STRUCTURED SUMMARY OF PROTEIN INTERACTIONS: TthCasB and TthCasBbind by x-ray crystallography (View interaction) TfuCasB1 and TfuCasB1bind by molecular sieving (View Interaction: 1, 2).

-	~~{{STRUCTURE_4h7a\| PDB=4h7a \| SCENE= }}~~	+	Nucleic acid binding surface and dimer interface revealed by CRISPR-associated CasB protein structures.,Nam KH, Huang Q, Ke A FEBS Lett. 2012 Nov 16;586(22):3956-61. doi: 10.1016/j.febslet.2012.09.041. Epub , 2012 Oct 16. PMID:23079036<ref>PMID:23079036</ref>

-	~~===Crystal structure~~ of ~~CasB from Thermus thermophilus===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

-	~~{{ABSTRACT_PUBMED_23079036}}~~	+	==See Also==
-		+	*[[Endonuclease\|Endonuclease]]
-	==~~About this Structure~~==	+	== References ==
-	[[~~4h7a~~]] ~~is a 2 chain structure with sequence from [http:~~//~~en.wikipedia.org/wiki/Thermus_thermophilus_hb8 Thermus thermophilus hb8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H7A OCA].~~	+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Thermus thermophilus hb8]]		[[Category: Thermus thermophilus hb8]]
-	[[Category: Ke, A.]]	+	[[Category: Ke, A]]
-	[[Category: Nam, K H.]]	+	[[Category: Nam, K H]]
	[[Category: Casb]]		[[Category: Casb]]
	[[Category: Cascade]]		[[Category: Cascade]]

OCA at 14:21, 28 November 2012

OCA — Wed, 28 Nov 2012 14:21:53 GMT

←Older revision		Revision as of 14:21, 28 November 2012
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-	[[Image:4h7a.~~jpg~~\|left\|200px]]	+	[[Image:4h7a.png\|left\|200px]]

	{{STRUCTURE_4h7a\| PDB=4h7a \| SCENE= }}		{{STRUCTURE_4h7a\| PDB=4h7a \| SCENE= }}
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	===Crystal structure of CasB from Thermus thermophilus===		===Crystal structure of CasB from Thermus thermophilus===

		+	{{ABSTRACT_PUBMED_23079036}}

	==About this Structure==		==About this Structure==
-	[[4h7a]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Thermus_thermophilus~~ Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H7A OCA].	+	[[4h7a]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus_hb8 Thermus thermophilus hb8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H7A OCA].
-	[[Category: Thermus thermophilus]]	+	[[Category: Thermus thermophilus hb8]]
	[[Category: Ke, A.]]		[[Category: Ke, A.]]
	[[Category: Nam, K H.]]		[[Category: Nam, K H.]]

OCA at 06:31, 3 October 2012

OCA — Wed, 03 Oct 2012 06:31:11 GMT

←Older revision		Revision as of 06:31, 3 October 2012
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-	~~'''Unreleased structure'''~~	+	[[Image:4h7a.jpg\|left\|200px]]

-	~~The entry~~ 4h7a ~~is ON HOLD~~	+	{{STRUCTURE_4h7a\| PDB=4h7a \| SCENE= }}

-	~~Authors: Ke, A., Nam, K.H.~~	+	===Crystal structure of CasB from Thermus thermophilus===

-	~~Description: Crystal~~ structure ~~of CasB~~ from Thermus thermophilus	+
		+	==About this Structure==
		+	[[4h7a]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H7A OCA].
		+	[[Category: Thermus thermophilus]]
		+	[[Category: Ke, A.]]
		+	[[Category: Nam, K H.]]
		+	[[Category: Casb]]
		+	[[Category: Cascade]]
		+	[[Category: Crispr]]
		+	[[Category: Crispr-assoicated protein]]
		+	[[Category: Dna binding protein]]
		+	[[Category: Nucleic acid binding protein]]

OCA: Protected "4h7a" [edit=sysop:move=sysop]

OCA — Wed, 26 Sep 2012 20:15:00 GMT

Protected "4h7a" [edit=sysop:move=sysop]

←Older revision

Revision as of 20:15, 26 September 2012

OCA: New page: '''Unreleased structure''' The entry 4h7a is ON HOLD Authors: Ke, A., Nam, K.H. Description: Crystal structure of CasB from Thermus thermophilus

OCA — Wed, 26 Sep 2012 20:14:59 GMT

New page: '''Unreleased structure''' The entry 4h7a is ON HOLD Authors: Ke, A., Nam, K.H. Description: Crystal structure of CasB from Thermus thermophilus

New page

'''Unreleased structure'''

The entry 4h7a is ON HOLD

Authors: Ke, A., Nam, K.H.

Description: Crystal structure of CasB from Thermus thermophilus