4h9w - Revision history

OCA at 15:02, 20 September 2023

OCA — Wed, 20 Sep 2023 15:02:11 GMT

←Older revision		Revision as of 15:02, 20 September 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4h9w]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Psophocarpus_tetragonolobus Psophocarpus tetragonolobus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H9W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4H9W FirstGlance]. <br>		<table><tr><td colspan='2'>[[4h9w]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Psophocarpus_tetragonolobus Psophocarpus tetragonolobus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H9W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4H9W FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4h9w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4h9w OCA], [https://pdbe.org/4h9w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4h9w RCSB], [https://www.ebi.ac.uk/pdbsum/4h9w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4h9w ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.5Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4h9w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4h9w OCA], [https://pdbe.org/4h9w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4h9w RCSB], [https://www.ebi.ac.uk/pdbsum/4h9w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4h9w ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 08:06, 3 November 2022

OCA — Thu, 03 Nov 2022 08:06:32 GMT

←Older revision		Revision as of 08:06, 3 November 2022
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	<StructureSection load='4h9w' size='340' side='right'caption='[[4h9w]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='4h9w' size='340' side='right'caption='[[4h9w]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4h9w]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Dolichos_tetragonolobus Dolichos~~ tetragonolobus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H9W OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4H9W FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4h9w]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Psophocarpus_tetragonolobus Psophocarpus tetragonolobus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H9W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4H9W FirstGlance]. <br>
-	</td></tr>~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ha2\|4ha2]]</td></tr>~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4h9w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4h9w OCA], [https://pdbe.org/4h9w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4h9w RCSB], [https://www.ebi.ac.uk/pdbsum/4h9w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4h9w ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4h9w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4h9w OCA], [~~http~~://pdbe.org/4h9w PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4h9w RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4h9w PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4h9w ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/ICW3_PSOTE ICW3_PSOTE]] Inhibits alpha-chymotrypsin at the molar ratio of 1:2 in state of 1:1.	+	[https://www.uniprot.org/uniprot/ICW3_PSOTE ICW3_PSOTE] Inhibits alpha-chymotrypsin at the molar ratio of 1:2 in state of 1:1.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	==See Also==		==See Also==
-	*[[Chymotrypsin ~~Inhibitor~~\|Chymotrypsin ~~Inhibitor~~]]	+	*[[Chymotrypsin inhibitor 3D structures\|Chymotrypsin inhibitor 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Dolichos tetragonolobus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Majumder, S~~]]	+	[[Category: Psophocarpus tetragonolobus]]
-	[[Category: ~~Sen, U~~]]	+	[[Category: Majumder S]]
-	[[Category: ~~Chymotrypsin inhibitor]]~~	+	[[Category: Sen U]]
-	~~[[Category: Hydrolase inhibitor]]~~	+
-	~~[[Category: Inhibitor of chymotrypsin~~]]	+

OCA at 07:55, 27 March 2019

OCA — Wed, 27 Mar 2019 07:55:23 GMT

←Older revision		Revision as of 07:55, 27 March 2019
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	==crystal structure of a METHIONINE mutant of WCI==		==crystal structure of a METHIONINE mutant of WCI==
-	<StructureSection load='4h9w' size='340' side='right' caption='[[4h9w]], [[Resolution\|resolution]] 2.50Å' scene=''>	+	<StructureSection load='4h9w' size='340' side='right'caption='[[4h9w]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4h9w]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Dolichos_tetragonolobus Dolichos tetragonolobus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H9W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4H9W FirstGlance]. <br>		<table><tr><td colspan='2'>[[4h9w]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Dolichos_tetragonolobus Dolichos tetragonolobus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H9W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4H9W FirstGlance]. <br>
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	</StructureSection>		</StructureSection>
	[[Category: Dolichos tetragonolobus]]		[[Category: Dolichos tetragonolobus]]
		+	[[Category: Large Structures]]
	[[Category: Majumder, S]]		[[Category: Majumder, S]]
	[[Category: Sen, U]]		[[Category: Sen, U]]

OCA at 09:10, 4 August 2016

OCA — Thu, 04 Aug 2016 09:10:38 GMT

←Older revision		Revision as of 09:10, 4 August 2016
Line 1:		Line 1:
		+
	==crystal structure of a METHIONINE mutant of WCI==		==crystal structure of a METHIONINE mutant of WCI==
	<StructureSection load='4h9w' size='340' side='right' caption='[[4h9w]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='4h9w' size='340' side='right' caption='[[4h9w]], [[Resolution\|resolution]] 2.50Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[4h9w]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Dolichos_tetragonolobus Dolichos tetragonolobus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H9W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4H9W FirstGlance]. <br>		<table><tr><td colspan='2'>[[4h9w]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Dolichos_tetragonolobus Dolichos tetragonolobus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H9W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4H9W FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ha2\|4ha2]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ha2\|4ha2]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4h9w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4h9w OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4h9w RCSB], [http://www.ebi.ac.uk/pdbsum/4h9w PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4h9w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4h9w OCA], [http://pdbe.org/4h9w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4h9w RCSB], [http://www.ebi.ac.uk/pdbsum/4h9w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4h9w ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4h9w" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Chymotrypsin Inhibitor\|Chymotrypsin Inhibitor]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 10:51, 24 December 2014

OCA — Wed, 24 Dec 2014 10:51:41 GMT

←Older revision		Revision as of 10:51, 24 December 2014
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-	~~{{STRUCTURE_4h9w\| PDB=4h9w \| SCENE= }}~~	+	==crystal structure of a METHIONINE mutant of WCI==
-	===crystal structure of a METHIONINE mutant of WCI===	+	<StructureSection load='4h9w' size='340' side='right' caption='[[4h9w]], [[Resolution\|resolution]] 2.50Å' scene=''>
-		+	== Structural highlights ==
-	==Function==	+	<table><tr><td colspan='2'>[[4h9w]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Dolichos_tetragonolobus Dolichos tetragonolobus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H9W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4H9W FirstGlance]. <br>
		+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ha2\|4ha2]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4h9w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4h9w OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4h9w RCSB], [http://www.ebi.ac.uk/pdbsum/4h9w PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
	[[http://www.uniprot.org/uniprot/ICW3_PSOTE ICW3_PSOTE]] Inhibits alpha-chymotrypsin at the molar ratio of 1:2 in state of 1:1.		[[http://www.uniprot.org/uniprot/ICW3_PSOTE ICW3_PSOTE]] Inhibits alpha-chymotrypsin at the molar ratio of 1:2 in state of 1:1.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	beta-trefoil fold, consisting of a six stranded beta-barrel capped at one end by a lid comprising of another six beta-strands, is one of the most important folds among proteins. Important classes of proteins like Interleukins (ILs), Fibroblast Growth Factors (FGFs), Kunitz (STI) family of inhibitors etc. belong to this fold. Their core is packed by hydrophobic residues contributed by the 6 stranded beta-barrel and three beta-hairpins that make essential contacts with each other and keep the protein in 'topologically minimal frustrated state'. A complete database analysis of the core residues of the beta-trefoil fold proteins presented here identified a conserved tryptophan (W91) residue in the Kunitz (STI) family of inhibitors that projects from the lid and interacts with the bottom layer residues of the barrel. This kind of interactions is unique in Kunitz (STI) family because no other families of beta-trefoil fold have such a shear sized residue at the barrel lid junction; suggesting its possible importance in packing and stability. We took WCI as a representative of this family and prepared four cavity creating mutants W91F-WCI, W91M-WCI, W91I-WCI & W91A-WCI. CD experiments show that the secondary structure of the mutants remains indistinguishable with the wild type. Crystal structures of the mutants W91F-WCI, W91M-WCI & W91A-WCI also show the same feature. However, slight readjustments of the side chains around the site of mutation have been observed so as to minimize the cavity created due to mutation. Comparative stability of these mutants, estimated using heat denaturation CD spectroscopy, indicates that stability of the mutants inversely correlates with the size of the cavity inside the core. Interestingly, although we mutated at the core, mutants show varying susceptibility against tryptic digestion that grossly follow their instability determined by CD. Our findings suggest that the W91 residue plays an important role in determining the stability and packing of the core of WCI.
		+
		+	A conserved tryptophan (W91) at the barrel-lid junction modulates the packing and stability of Kunitz (STI) family of inhibitors.,Majumder S, Khamrui S, Banerjee R, Bhowmik P, Sen U Biochim Biophys Acta. 2015 Jan;1854(1):55-64. doi: 10.1016/j.bbapap.2014.10.021. , Epub 2014 Oct 31. PMID:25448016<ref>PMID:25448016</ref>

-	==~~About this Structure~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[~~4h9w]] is a 1 chain structure. Full crystallographic information is available from [http~~:~~//oca.weizmann.ac.il/oca-bin/ocashort?id=4H9W OCA~~].	+	</div>
-	[[Category: Majumder, S.]]	+	== References ==
-	[[Category: Sen, U.]]	+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Dolichos tetragonolobus]]
		+	[[Category: Majumder, S]]
		+	[[Category: Sen, U]]
	[[Category: Chymotrypsin inhibitor]]		[[Category: Chymotrypsin inhibitor]]
	[[Category: Hydrolase inhibitor]]		[[Category: Hydrolase inhibitor]]
	[[Category: Inhibitor of chymotrypsin]]		[[Category: Inhibitor of chymotrypsin]]

OCA at 06:40, 9 April 2014

OCA — Wed, 09 Apr 2014 06:40:12 GMT

←Older revision		Revision as of 06:40, 9 April 2014
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-	~~'''Unreleased~~ structure~~'''~~	+	{{STRUCTURE_4h9w\| PDB=4h9w \| SCENE= }}
		+	===crystal structure of a METHIONINE mutant of WCI===

-	~~The entry 4h9w is ON HOLD until Paper Publication~~	+	==Function==
		+	[[http://www.uniprot.org/uniprot/ICW3_PSOTE ICW3_PSOTE]] Inhibits alpha-chymotrypsin at the molar ratio of 1:2 in state of 1:1.

-	~~Authors~~: Majumder, S., Sen, U.	+	==About this Structure==
-		+	[[4h9w]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H9W OCA].
-	~~Description~~: ~~crystal structure of a METHIONINE mutant~~ of ~~WCI~~	+	[[Category: Majumder, S.]]
		+	[[Category: Sen, U.]]
		+	[[Category: Chymotrypsin inhibitor]]
		+	[[Category: Hydrolase inhibitor]]
		+	[[Category: Inhibitor of chymotrypsin]]

OCA at 09:47, 13 March 2013

OCA — Wed, 13 Mar 2013 09:47:48 GMT

←Older revision		Revision as of 09:47, 13 March 2013
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4h9w is ON HOLD	+	The entry 4h9w is ON HOLD until Paper Publication

	Authors: Majumder, S., Sen, U.		Authors: Majumder, S., Sen, U.

	Description: crystal structure of a METHIONINE mutant of WCI		Description: crystal structure of a METHIONINE mutant of WCI

OCA at 07:08, 24 October 2012

OCA — Wed, 24 Oct 2012 07:08:35 GMT

←Older revision		Revision as of 07:08, 24 October 2012
Line 3:		Line 3:
	The entry 4h9w is ON HOLD		The entry 4h9w is ON HOLD

-	Authors: Majumder, ~~Sudip~~, Sen, ~~Udayaditya~~	+	Authors: Majumder, S., Sen, U.

	Description: crystal structure of a METHIONINE mutant of WCI		Description: crystal structure of a METHIONINE mutant of WCI

OCA: Protected "4h9w" [edit=sysop:move=sysop]

OCA — Wed, 03 Oct 2012 05:50:59 GMT

Protected "4h9w" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:50, 3 October 2012

OCA: New page: '''Unreleased structure''' The entry 4h9w is ON HOLD Authors: Majumder, Sudip, Sen, Udayaditya Description: crystal structure of a METHIONINE mutant of WCI

OCA — Wed, 03 Oct 2012 05:50:58 GMT

New page: '''Unreleased structure''' The entry 4h9w is ON HOLD Authors: Majumder, Sudip, Sen, Udayaditya Description: crystal structure of a METHIONINE mutant of WCI

New page

'''Unreleased structure'''

The entry 4h9w is ON HOLD

Authors: Majumder, Sudip, Sen, Udayaditya

Description: crystal structure of a METHIONINE mutant of WCI