4hb1 - Revision history

OCA at 11:36, 1 March 2024

OCA — Fri, 01 Mar 2024 11:36:23 GMT

←Older revision		Revision as of 11:36, 1 March 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4hb1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HB1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4HB1 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4hb1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HB1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4HB1 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=UNX:UNKNOWN+ATOM+OR+ION'>UNX</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.9Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=UNX:UNKNOWN+ATOM+OR+ION'>UNX</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4hb1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hb1 OCA], [https://pdbe.org/4hb1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4hb1 RCSB], [https://www.ebi.ac.uk/pdbsum/4hb1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4hb1 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4hb1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hb1 OCA], [https://pdbe.org/4hb1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4hb1 RCSB], [https://www.ebi.ac.uk/pdbsum/4hb1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4hb1 ProSAT]</span></td></tr>
	</table>		</table>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	A 108 amino acid protein was designed and constructed from a reduced alphabet of seven amino acids. The 2.9 A resolution X-ray crystal structure confirms that the protein is a four helix bundle, as it was designed to be. Hydrogen/deuterium exchange experiments reveal buried amide protons with protection factors in excess of 1 x 10(6) in the range characteristic of well protected protons in functional folded proteins (10(3)-10(8)) rather than protons in rapid exchange (0-10(2)). The protein is monomeric at 1 mM, the concentration at which the exchange experiments were undertaken, indicating that the exchange factors are due to a unique stable tertiary structure fold, and not due to any higher order quaternary structure. Thermodynamic analysis provides an estimate of the free energy of folding of -9.3 kcal mole-1 at 25 degrees C, consistent with the free energy of folding derived from the protection factors of the most protected protons, indicating that global unfolding is required for exchange of the most protected protons.
-
-	A designed four helix bundle protein with native-like structure.,Schafmeister CE, LaPorte SL, Miercke LJ, Stroud RM Nat Struct Biol. 1997 Dec;4(12):1039-46. PMID:9406555<ref>PMID:9406555</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 4hb1" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 08:08, 3 November 2022

OCA — Thu, 03 Nov 2022 08:08:06 GMT

←Older revision		Revision as of 08:08, 3 November 2022
Line 1:		Line 1:

	==A DESIGNED FOUR HELIX BUNDLE PROTEIN.==		==A DESIGNED FOUR HELIX BUNDLE PROTEIN.==
-	<StructureSection load='4hb1' size='340' side='right' caption='[[4hb1]], [[Resolution\|resolution]] 2.90Å' scene=''>	+	<StructureSection load='4hb1' size='340' side='right'caption='[[4hb1]], [[Resolution\|resolution]] 2.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4hb1]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Synthetic_construct_sequences~~ Synthetic construct ~~sequences~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HB1 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4HB1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4hb1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HB1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4HB1 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNX:UNKNOWN+ATOM+OR+ION'>UNX</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=UNX:UNKNOWN+ATOM+OR+ION'>UNX</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SYNTHETIC GENE DHP1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=32630 SYNTHETIC CONSTRUCT sequences])</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4hb1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hb1 OCA], [https://pdbe.org/4hb1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4hb1 RCSB], [https://www.ebi.ac.uk/pdbsum/4hb1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4hb1 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4hb1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hb1 OCA], [~~http~~://pdbe.org/4hb1 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4hb1 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4hb1 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4hb1 ProSAT]</span></td></tr>	+
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 21:		Line 20:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Synthetic construct sequences~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Laporte, S L~~]]	+	[[Category: Synthetic construct]]
-	[[Category: ~~Miercke, L J.W~~]]	+	[[Category: Laporte SL]]
-	[[Category: ~~Schafmeister, C E~~]]	+	[[Category: Miercke LJW]]
-	[[Category: ~~Stroud, R M~~]]	+	[[Category: Schafmeister CE]]
-	[[Category: ~~Designed helical bundle~~]]	+	[[Category: Stroud RM]]

OCA at 14:39, 11 August 2016

OCA — Thu, 11 Aug 2016 14:39:43 GMT

←Older revision		Revision as of 14:39, 11 August 2016
Line 1:		Line 1:
		+
	==A DESIGNED FOUR HELIX BUNDLE PROTEIN.==		==A DESIGNED FOUR HELIX BUNDLE PROTEIN.==
	<StructureSection load='4hb1' size='340' side='right' caption='[[4hb1]], [[Resolution\|resolution]] 2.90Å' scene=''>		<StructureSection load='4hb1' size='340' side='right' caption='[[4hb1]], [[Resolution\|resolution]] 2.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4hb1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Synthetic_construct~~ Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HB1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HB1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4hb1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Synthetic_construct_sequences Synthetic construct sequences]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HB1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HB1 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNX:UNKNOWN+ATOM+OR+ION'>UNX</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNX:UNKNOWN+ATOM+OR+ION'>UNX</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SYNTHETIC GENE DHP1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=32630 ~~synthetic construct~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SYNTHETIC GENE DHP1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=32630 SYNTHETIC CONSTRUCT sequences])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hb1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hb1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4hb1 RCSB], [http://www.ebi.ac.uk/pdbsum/4hb1 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hb1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hb1 OCA], [http://pdbe.org/4hb1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4hb1 RCSB], [http://www.ebi.ac.uk/pdbsum/4hb1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4hb1 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 15:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4hb1" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Synthetic construct]]	+	[[Category: Synthetic construct sequences]]
	[[Category: Laporte, S L]]		[[Category: Laporte, S L]]
	[[Category: Miercke, L J.W]]		[[Category: Miercke, L J.W]]

OCA at 13:01, 4 January 2015

OCA — Sun, 04 Jan 2015 13:01:20 GMT

←Older revision		Revision as of 13:01, 4 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4hb1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HB1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HB1 FirstGlance]. <br>		<table><tr><td colspan='2'>[[4hb1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HB1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HB1 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNX:UNKNOWN+ATOM+OR+ION'>UNX</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNX:UNKNOWN+ATOM+OR+ION'>UNX</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SYNTHETIC GENE DHP1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=32630 synthetic construct])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SYNTHETIC GENE DHP1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=32630 synthetic construct])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hb1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hb1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4hb1 RCSB], [http://www.ebi.ac.uk/pdbsum/4hb1 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hb1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hb1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4hb1 RCSB], [http://www.ebi.ac.uk/pdbsum/4hb1 PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 20:		Line 20:
	</StructureSection>		</StructureSection>
	[[Category: Synthetic construct]]		[[Category: Synthetic construct]]
-	[[Category: Laporte, S L.]]	+	[[Category: Laporte, S L]]
-	[[Category: Miercke, L J.W.]]	+	[[Category: Miercke, L J.W]]
-	[[Category: Schafmeister, C E.]]	+	[[Category: Schafmeister, C E]]
-	[[Category: Stroud, R M.]]	+	[[Category: Stroud, R M]]
	[[Category: Designed helical bundle]]		[[Category: Designed helical bundle]]

OCA at 10:23, 16 June 2014

OCA — Mon, 16 Jun 2014 10:23:36 GMT

←Older revision		Revision as of 10:23, 16 June 2014
Line 1:		Line 1:
-	[[~~Image:~~4hb1.~~png~~\|~~left~~\|~~200px~~]]	+	==A DESIGNED FOUR HELIX BUNDLE PROTEIN.==
		+	<StructureSection load='4hb1' size='340' side='right' caption='[[4hb1]], [[Resolution\|resolution]] 2.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4hb1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HB1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HB1 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNX:UNKNOWN+ATOM+OR+ION'>UNX</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SYNTHETIC GENE DHP1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=32630 synthetic construct])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hb1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hb1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4hb1 RCSB], [http://www.ebi.ac.uk/pdbsum/4hb1 PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	A 108 amino acid protein was designed and constructed from a reduced alphabet of seven amino acids. The 2.9 A resolution X-ray crystal structure confirms that the protein is a four helix bundle, as it was designed to be. Hydrogen/deuterium exchange experiments reveal buried amide protons with protection factors in excess of 1 x 10(6) in the range characteristic of well protected protons in functional folded proteins (10(3)-10(8)) rather than protons in rapid exchange (0-10(2)). The protein is monomeric at 1 mM, the concentration at which the exchange experiments were undertaken, indicating that the exchange factors are due to a unique stable tertiary structure fold, and not due to any higher order quaternary structure. Thermodynamic analysis provides an estimate of the free energy of folding of -9.3 kcal mole-1 at 25 degrees C, consistent with the free energy of folding derived from the protection factors of the most protected protons, indicating that global unfolding is required for exchange of the most protected protons.

-	~~{{STRUCTURE_4hb1\| PDB=4hb1 \| SCENE= }}~~	+	A designed four helix bundle protein with native-like structure.,Schafmeister CE, LaPorte SL, Miercke LJ, Stroud RM Nat Struct Biol. 1997 Dec;4(12):1039-46. PMID:9406555<ref>PMID:9406555</ref>

-	~~===A DESIGNED FOUR HELIX BUNDLE PROTEIN.===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_9406555}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[4hb1]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Synthetic_construct Synthetic construct]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=4HB1 OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:009406555<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Synthetic construct]]		[[Category: Synthetic construct]]
	[[Category: Laporte, S L.]]		[[Category: Laporte, S L.]]

OCA: Protected "4hb1" [edit=sysop:move=sysop]

OCA — Tue, 14 Aug 2012 15:58:00 GMT

Protected "4hb1" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:58, 14 August 2012

OCA at 15:57, 14 August 2012

OCA — Tue, 14 Aug 2012 15:57:58 GMT

←Older revision		Revision as of 15:57, 14 August 2012
Line 1:		Line 1:
-	{{Seed}}
	[[Image:4hb1.png\|left\|200px]]		[[Image:4hb1.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_4hb1", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_4hb1\| PDB=4hb1 \| SCENE= }}		{{STRUCTURE_4hb1\| PDB=4hb1 \| SCENE= }}

	===A DESIGNED FOUR HELIX BUNDLE PROTEIN.===		===A DESIGNED FOUR HELIX BUNDLE PROTEIN.===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_9406555}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 9406555 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_9406555}}		{{ABSTRACT_PUBMED_9406555}}

	==About this Structure==		==About this Structure==
-	Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HB1 OCA].	+	[[4hb1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HB1 OCA].

	==Reference==		==Reference==
-	~~A designed four helix bundle protein with native-like structure., Schafmeister CE, LaPorte SL, Miercke LJ, Stroud RM, Nat Struct Biol. 1997 Dec;4(12):1039-46.~~ PMID:[~~http~~:~~//www.ncbi.nlm.nih.gov/pubmed/9406555 9406555~~]	+	<ref group="xtra">PMID:009406555</ref><references group="xtra"/>
		+	[[Category: Synthetic construct]]
	[[Category: Laporte, S L.]]		[[Category: Laporte, S L.]]
	[[Category: Miercke, L J.W.]]		[[Category: Miercke, L J.W.]]
Line 29:		Line 18:
	[[Category: Stroud, R M.]]		[[Category: Stroud, R M.]]
	[[Category: Designed helical bundle]]		[[Category: Designed helical bundle]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jul 3 13:40:17 2008''

OCA at 10:40, 3 July 2008

OCA — Thu, 03 Jul 2008 10:40:18 GMT

←Older revision		Revision as of 10:40, 3 July 2008
Line 1:		Line 1:
-	[[Image:4hb1.~~jpg~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:4hb1.png\|left\|200px]]

	<!--		<!--
Line 9:		Line 10:
	{{STRUCTURE_4hb1\| PDB=4hb1 \| SCENE= }}		{{STRUCTURE_4hb1\| PDB=4hb1 \| SCENE= }}

-	~~'''~~A DESIGNED FOUR HELIX BUNDLE PROTEIN.~~'''~~	+	===A DESIGNED FOUR HELIX BUNDLE PROTEIN.===


-	~~==Overview==~~	+	<!--
-	~~A 108 amino acid protein was designed and constructed from a reduced alphabet of seven amino acids. The 2.9 A resolution X~~-ray crystal structure confirms that the protein is a four helix bundle, as it was designed to be. Hydrogen/deuterium exchange experiments reveal buried amide protons with protection factors in excess of 1 x 10(6) in the range characteristic of well protected protons in functional folded proteins (10(3)-~~10(8)) rather than protons in rapid exchange (0-10(2)).~~ The ~~protein is monomeric at 1 mM~~, ~~the concentration at which the exchange experiments were undertaken~~, ~~indicating that~~ the ~~exchange factors are due~~ to ~~a unique stable tertiary structure fold, and not due to any higher order quaternary structure. Thermodynamic analysis provides an estimate of~~ the ~~free energy of folding of -9~~.~~3 kcal mole-1 at 25 degrees C, consistent with the free energy of folding derived from the protection factors of the most protected protons~~, ~~indicating that global unfolding~~ is ~~required for exchange of~~ the ~~most protected protons~~.	+	The line below this paragraph, {{ABSTRACT_PUBMED_9406555}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 9406555 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_9406555}}

	==About this Structure==		==About this Structure==
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	[[Category: Stroud, R M.]]		[[Category: Stroud, R M.]]
	[[Category: Designed helical bundle]]		[[Category: Designed helical bundle]]
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sun May~~ ~~4 22~~:25:05 2008''	+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jul 3 13:40:17 2008''

OCA at 19:25, 4 May 2008

OCA — Sun, 04 May 2008 19:25:05 GMT

←Older revision		Revision as of 19:25, 4 May 2008
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	[[Image:4hb1.jpg\|left\|200px]]		[[Image:4hb1.jpg\|left\|200px]]

-	~~{{Structure~~	+	<!--
-	~~\|PDB= 4hb1 \|SIZE=350\|CAPTION= <scene name='initialview01'>4hb1</scene>~~, ~~resolution 2~~.~~9Å~~	+	The line below this paragraph, containing "STRUCTURE_4hb1", creates the "Structure Box" on the page.
-	~~\|SITE=~~	+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	~~\|LIGAND= <~~scene ~~name='pdbligand=UNX:UNKNOWN+ATOM+OR+ION'>UNX</scene>~~	+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	~~\|ACTIVITY=~~	+	or leave the SCENE parameter empty for the default display.
-	~~\|GENE= SYNTHETIC GENE DHP1 ([http://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=32630 synthetic construct])~~	+	-->
-	~~\|DOMAIN=~~	+	{{STRUCTURE_4hb1\| PDB=4hb1 \| SCENE= }}
-	\|~~RELATEDENTRY~~=	+
-	\|~~RESOURCES~~=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hb1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hb1 OCA], [http://www.ebi.ac.uk/pdbsum/4hb1 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=4hb1 RCSB]</span>	+
-	}}	+

	'''A DESIGNED FOUR HELIX BUNDLE PROTEIN.'''		'''A DESIGNED FOUR HELIX BUNDLE PROTEIN.'''
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	==About this Structure==		==About this Structure==
-	~~4HB1 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct].~~ Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HB1 OCA].	+	Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HB1 OCA].

	==Reference==		==Reference==
	A designed four helix bundle protein with native-like structure., Schafmeister CE, LaPorte SL, Miercke LJ, Stroud RM, Nat Struct Biol. 1997 Dec;4(12):1039-46. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9406555 9406555]		A designed four helix bundle protein with native-like structure., Schafmeister CE, LaPorte SL, Miercke LJ, Stroud RM, Nat Struct Biol. 1997 Dec;4(12):1039-46. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9406555 9406555]
-	[[Category: Protein complex]]
-	[[Category: Synthetic construct]]
	[[Category: Laporte, S L.]]		[[Category: Laporte, S L.]]
	[[Category: Miercke, L J.W.]]		[[Category: Miercke, L J.W.]]
	[[Category: Schafmeister, C E.]]		[[Category: Schafmeister, C E.]]
	[[Category: Stroud, R M.]]		[[Category: Stroud, R M.]]
-	[[Category: ~~designed~~ helical bundle]]	+	[[Category: Designed helical bundle]]
-		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 22:25:05 2008''
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Mon Mar 31 05~~:39:03 2008''	+

OCA at 02:39, 31 March 2008

OCA — Mon, 31 Mar 2008 02:39:05 GMT

←Older revision		Revision as of 02:39, 31 March 2008
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	\|PDB= 4hb1 \|SIZE=350\|CAPTION= <scene name='initialview01'>4hb1</scene>, resolution 2.9Å		\|PDB= 4hb1 \|SIZE=350\|CAPTION= <scene name='initialview01'>4hb1</scene>, resolution 2.9Å
	\|SITE=		\|SITE=
-	\|LIGAND= <scene name='pdbligand=UNX:UNKNOWN ATOM OR ION'>UNX</scene>	+	\|LIGAND= <scene name='pdbligand=UNX:UNKNOWN+ATOM+OR+ION'>UNX</scene>
	\|ACTIVITY=		\|ACTIVITY=
	\|GENE= SYNTHETIC GENE DHP1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=32630 synthetic construct])		\|GENE= SYNTHETIC GENE DHP1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=32630 synthetic construct])
		+	\|DOMAIN=
		+	\|RELATEDENTRY=
		+	\|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hb1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hb1 OCA], [http://www.ebi.ac.uk/pdbsum/4hb1 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=4hb1 RCSB]</span>
	}}		}}

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	[[Category: Schafmeister, C E.]]		[[Category: Schafmeister, C E.]]
	[[Category: Stroud, R M.]]		[[Category: Stroud, R M.]]
-	[[Category: UNX]]
	[[Category: designed helical bundle]]		[[Category: designed helical bundle]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Thu~~ Mar ~~20 19~~:09:52 2008''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:39:03 2008''