4hgp - Revision history

OCA at 15:05, 20 September 2023

OCA — Wed, 20 Sep 2023 15:05:23 GMT

←Older revision		Revision as of 15:05, 20 September 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4hgp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Haemophilus_influenzae_Rd_KW20 Haemophilus influenzae Rd KW20]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HGP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4HGP FirstGlance]. <br>		<table><tr><td colspan='2'>[[4hgp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Haemophilus_influenzae_Rd_KW20 Haemophilus influenzae Rd KW20]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HGP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4HGP FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=KDO:3-DEOXY-D-MANNO-OCT-2-ULOSONIC+ACID'>KDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=VN4:OXIDO(DIOXO)VANADIUM'>VN4</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.8Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=KDO:3-DEOXY-D-MANNO-OCT-2-ULOSONIC+ACID'>KDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=VN4:OXIDO(DIOXO)VANADIUM'>VN4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4hgp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hgp OCA], [https://pdbe.org/4hgp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4hgp RCSB], [https://www.ebi.ac.uk/pdbsum/4hgp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4hgp ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4hgp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hgp OCA], [https://pdbe.org/4hgp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4hgp RCSB], [https://www.ebi.ac.uk/pdbsum/4hgp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4hgp ProSAT]</span></td></tr>
	</table>		</table>

OCA at 08:15, 3 November 2022

OCA — Thu, 03 Nov 2022 08:15:43 GMT

←Older revision		Revision as of 08:15, 3 November 2022
Line 3:		Line 3:
	<StructureSection load='4hgp' size='340' side='right'caption='[[4hgp]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='4hgp' size='340' side='right'caption='[[4hgp]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4hgp]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Haein Haein~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HGP OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4HGP FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4hgp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Haemophilus_influenzae_Rd_KW20 Haemophilus influenzae Rd KW20]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HGP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4HGP FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=KDO:3-DEOXY-D-MANNO-OCT-2-ULOSONIC+ACID'>KDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=VN4:OXIDO(DIOXO)VANADIUM'>VN4</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=KDO:3-DEOXY-D-MANNO-OCT-2-ULOSONIC+ACID'>KDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=VN4:OXIDO(DIOXO)VANADIUM'>VN4</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1k1e\|1k1e]], [[4hgn\|4hgn]], [[4hgo\|4hgo]], [[4hgq\|4hgq]], [[4hgr\|4hgr]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4hgp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hgp OCA], [https://pdbe.org/4hgp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4hgp RCSB], [https://www.ebi.ac.uk/pdbsum/4hgp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4hgp ProSAT]</span></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">HI_1679 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=71421 HAEIN])</td></tr>~~	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/3-deoxy-manno-octulosonate-8-phosphatase 3-deoxy-manno-octulosonate-8-phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.45 3.1.3.45] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4hgp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hgp OCA], [~~http~~://pdbe.org/4hgp PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4hgp RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4hgp PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4hgp ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/KDSC_HAEIN KDSC_HAEIN]] Involved in the biosynthesis of lipopolysaccharides (LPSs), but is not essential. Catalyzes the hydrolysis of 3-deoxy-D-manno-octulosonate 8-phosphate (KDO 8-P) to 3-deoxy-D-manno-octulosonate (KDO) and inorganic phosphate.<ref>PMID:12639950</ref>	+	[https://www.uniprot.org/uniprot/KDSC_HAEIN KDSC_HAEIN] Involved in the biosynthesis of lipopolysaccharides (LPSs), but is not essential. Catalyzes the hydrolysis of 3-deoxy-D-manno-octulosonate 8-phosphate (KDO 8-P) to 3-deoxy-D-manno-octulosonate (KDO) and inorganic phosphate.<ref>PMID:12639950</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~3-deoxy-manno-octulosonate-8-phosphatase]]~~	+	[[Category: Haemophilus influenzae Rd KW20]]
-	~~[[Category: Haein~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Allen~~, K N~~]]	+	[[Category: Allen KN]]
-	[[Category: Daughtry~~, K D]]~~	+	[[Category: Daughtry KD]]
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Phosphohydroylase]]~~	+
-	~~[[Category: Rossmann fold~~]]	+

OCA at 08:20, 27 March 2019

OCA — Wed, 27 Mar 2019 08:20:41 GMT

←Older revision		Revision as of 08:20, 27 March 2019
Line 1:		Line 1:

	==Crystal Structure of 2-keto-3-deoxyoctulosonate 8-phosphate phosphohydrolase from Haemophilus influenzae in complex with transition state mimic==		==Crystal Structure of 2-keto-3-deoxyoctulosonate 8-phosphate phosphohydrolase from Haemophilus influenzae in complex with transition state mimic==
-	<StructureSection load='4hgp' size='340' side='right' caption='[[4hgp]], [[Resolution\|resolution]] 1.80Å' scene=''>	+	<StructureSection load='4hgp' size='340' side='right'caption='[[4hgp]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4hgp]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HGP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HGP FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4hgp]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Haein Haein]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HGP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HGP FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=KDO:3-DEOXY-D-MANNO-OCT-2-ULOSONIC+ACID'>KDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=VN4:OXIDO(DIOXO)VANADIUM'>VN4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=KDO:3-DEOXY-D-MANNO-OCT-2-ULOSONIC+ACID'>KDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=VN4:OXIDO(DIOXO)VANADIUM'>VN4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1k1e\|1k1e]], [[4hgn\|4hgn]], [[4hgo\|4hgo]], [[4hgq\|4hgq]], [[4hgr\|4hgr]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1k1e\|1k1e]], [[4hgn\|4hgn]], [[4hgo\|4hgo]], [[4hgq\|4hgq]], [[4hgr\|4hgr]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">HI_1679 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=71421 HAEIN])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/3-deoxy-manno-octulosonate-8-phosphatase 3-deoxy-manno-octulosonate-8-phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.45 3.1.3.45] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/3-deoxy-manno-octulosonate-8-phosphatase 3-deoxy-manno-octulosonate-8-phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.45 3.1.3.45] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hgp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hgp OCA], [http://pdbe.org/4hgp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4hgp RCSB], [http://www.ebi.ac.uk/pdbsum/4hgp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4hgp ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hgp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hgp OCA], [http://pdbe.org/4hgp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4hgp RCSB], [http://www.ebi.ac.uk/pdbsum/4hgp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4hgp ProSAT]</span></td></tr>
Line 25:		Line 26:
	</StructureSection>		</StructureSection>
	[[Category: 3-deoxy-manno-octulosonate-8-phosphatase]]		[[Category: 3-deoxy-manno-octulosonate-8-phosphatase]]
		+	[[Category: Haein]]
		+	[[Category: Large Structures]]
	[[Category: Allen, K N]]		[[Category: Allen, K N]]
	[[Category: Daughtry, K D]]		[[Category: Daughtry, K D]]

OCA at 02:24, 5 August 2016

OCA — Fri, 05 Aug 2016 02:24:30 GMT

←Older revision		Revision as of 02:24, 5 August 2016
Line 1:		Line 1:
		+
	==Crystal Structure of 2-keto-3-deoxyoctulosonate 8-phosphate phosphohydrolase from Haemophilus influenzae in complex with transition state mimic==		==Crystal Structure of 2-keto-3-deoxyoctulosonate 8-phosphate phosphohydrolase from Haemophilus influenzae in complex with transition state mimic==
	<StructureSection load='4hgp' size='340' side='right' caption='[[4hgp]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='4hgp' size='340' side='right' caption='[[4hgp]], [[Resolution\|resolution]] 1.80Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1k1e\|1k1e]], [[4hgn\|4hgn]], [[4hgo\|4hgo]], [[4hgq\|4hgq]], [[4hgr\|4hgr]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1k1e\|1k1e]], [[4hgn\|4hgn]], [[4hgo\|4hgo]], [[4hgq\|4hgq]], [[4hgr\|4hgr]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/3-deoxy-manno-octulosonate-8-phosphatase 3-deoxy-manno-octulosonate-8-phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.45 3.1.3.45] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/3-deoxy-manno-octulosonate-8-phosphatase 3-deoxy-manno-octulosonate-8-phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.45 3.1.3.45] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hgp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hgp OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4hgp RCSB], [http://www.ebi.ac.uk/pdbsum/4hgp PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hgp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hgp OCA], [http://pdbe.org/4hgp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4hgp RCSB], [http://www.ebi.ac.uk/pdbsum/4hgp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4hgp ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4hgp" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 15:05, 25 December 2014

OCA — Thu, 25 Dec 2014 15:05:07 GMT

←Older revision		Revision as of 15:05, 25 December 2014
Line 8:		Line 8:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hgp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hgp OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4hgp RCSB], [http://www.ebi.ac.uk/pdbsum/4hgp PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hgp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hgp OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4hgp RCSB], [http://www.ebi.ac.uk/pdbsum/4hgp PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/KDSC_HAEIN KDSC_HAEIN]] Involved in the biosynthesis of lipopolysaccharides (LPSs), but is not essential. Catalyzes the hydrolysis of 3-deoxy-D-manno-octulosonate 8-phosphate (KDO 8-P) to 3-deoxy-D-manno-octulosonate (KDO) and inorganic phosphate.<ref>PMID:12639950</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 07:35, 12 November 2014

OCA — Wed, 12 Nov 2014 07:35:30 GMT

←Older revision		Revision as of 07:35, 12 November 2014
Line 2:		Line 2:
	<StructureSection load='4hgp' size='340' side='right' caption='[[4hgp]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='4hgp' size='340' side='right' caption='[[4hgp]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4hgp]] is a 1 chain structure ~~with sequence from [http://en.wikipedia.org/wiki/Haemophilus_influenzae_rd_kw20 Haemophilus influenzae rd kw20]~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HGP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HGP FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4hgp]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HGP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HGP FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=KDO:3-DEOXY-D-MANNO-OCT-2-ULOSONIC+ACID'>KDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=VN4:OXIDO(DIOXO)VANADIUM'>VN4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=KDO:3-DEOXY-D-MANNO-OCT-2-ULOSONIC+ACID'>KDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=VN4:OXIDO(DIOXO)VANADIUM'>VN4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1k1e\|1k1e]], [[4hgn\|4hgn]], [[4hgo\|4hgo]], [[4hgq\|4hgq]], [[4hgr\|4hgr]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1k1e\|1k1e]], [[4hgn\|4hgn]], [[4hgo\|4hgo]], [[4hgq\|4hgq]], [[4hgr\|4hgr]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">HI_1679 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=71421 Haemophilus influenzae Rd KW20])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/3-deoxy-manno-octulosonate-8-phosphatase 3-deoxy-manno-octulosonate-8-phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.45 3.1.3.45] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/3-deoxy-manno-octulosonate-8-phosphatase 3-deoxy-manno-octulosonate-8-phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.45 3.1.3.45] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hgp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hgp OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4hgp RCSB], [http://www.ebi.ac.uk/pdbsum/4hgp PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hgp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hgp OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4hgp RCSB], [http://www.ebi.ac.uk/pdbsum/4hgp PDBsum]</span></td></tr>
Line 22:		Line 21:
	</StructureSection>		</StructureSection>
	[[Category: 3-deoxy-manno-octulosonate-8-phosphatase]]		[[Category: 3-deoxy-manno-octulosonate-8-phosphatase]]
-	~~[[Category: Haemophilus influenzae rd kw20]]~~	+	[[Category: Allen, K N]]
-	[[Category: Allen, K N.]]	+	[[Category: Daughtry, K D]]
-	[[Category: Daughtry, K D.]]	+
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Phosphohydroylase]]		[[Category: Phosphohydroylase]]
	[[Category: Rossmann fold]]		[[Category: Rossmann fold]]

OCA at 14:21, 3 November 2014

OCA — Mon, 03 Nov 2014 14:21:50 GMT

←Older revision		Revision as of 14:21, 3 November 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_4hgp\| PDB=4hgp \| SCENE= }}~~	+	==Crystal Structure of 2-keto-3-deoxyoctulosonate 8-phosphate phosphohydrolase from Haemophilus influenzae in complex with transition state mimic==
-	===Crystal Structure of 2-keto-3-deoxyoctulosonate 8-phosphate phosphohydrolase from Haemophilus influenzae in complex with transition state mimic===	+	<StructureSection load='4hgp' size='340' side='right' caption='[[4hgp]], [[Resolution\|resolution]] 1.80Å' scene=''>
-	~~{{ABSTRACT_PUBMED_23848398}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4hgp]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Haemophilus_influenzae_rd_kw20 Haemophilus influenzae rd kw20]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HGP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HGP FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=KDO:3-DEOXY-D-MANNO-OCT-2-ULOSONIC+ACID'>KDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=VN4:OXIDO(DIOXO)VANADIUM'>VN4</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1k1e\|1k1e]], [[4hgn\|4hgn]], [[4hgo\|4hgo]], [[4hgq\|4hgq]], [[4hgr\|4hgr]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">HI_1679 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=71421 Haemophilus influenzae Rd KW20])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/3-deoxy-manno-octulosonate-8-phosphatase 3-deoxy-manno-octulosonate-8-phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.45 3.1.3.45] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hgp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hgp OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4hgp RCSB], [http://www.ebi.ac.uk/pdbsum/4hgp PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The haloacid dehalogenase enzyme superfamily (HADSF) is largely composed of phosphatases that have been particularly successful at adaptating to novel biological functions relative to members of other phosphatase families. Herein, we examine the structural basis for the divergence of function in two bacterial homologues: 2-keto-3-deoxy-d-manno-octulosonate 8-phosphate phosphohydrolase (KDO8P phosphatase, KDO8PP) and 2-keto-3-deoxy-9-O-phosphonononic acid phosphohydrolase (KDN9P phosphatase, KDN9PP). KDO8PP and KDN9PP catalyze the final step in KDO and KDN synthesis, respectively, prior to transfer to CMP to form the activated sugar nucleotide. KDO8PP and KDN9PP orthologs derived from an evolutionarily diverse collection of bacterial species were subjected to steady-state kinetic analysis to determine their specificities toward catalyzed KDO8P and KDN9P hydrolysis. Although each enzyme was more active with its biological substrate, the degree of selectivity (as defined by the ratio of kcat/Km for KDO8P vs KDN9P) varied significantly. High-resolution X-ray structure determination of Haemophilus influenzae KDO8PP bound to KDO/VO3(-) and Bacteriodes thetaiotaomicron KDN9PP bound to KDN/VO3(-) revealed the substrate-binding residues. The structures of the KDO8PP and KDN9PP orthologs were also determined to reveal the differences in their active-site structures that underlie the variation in substrate preference. Bioinformatic analysis was carried out to define the sequence divergence among KDN9PP and KDO8PP orthologs. The KDN9PP orthologs were found to exist as single-domain proteins or fused with the pathway nucleotidyl transferases; the fusion of KDO8PP with the transferase is rare. The KDO8PP and KDN9PP orthologs share a stringently conserved Arg residue that forms a salt bridge with the substrate carboxylate group. The split of the KDN9PP lineage from the KDO8PP orthologs is easily tracked by the acquisition of a Glu/Lys pair that supports KDN9P binding. Moreover, independently evolved lineages of KDO8PP orthologs exist, and are separated by diffuse active-site sequence boundaries. We infer a high tolerance of the KDO8PP catalytic platform to amino acid replacements that in turn influence substrate specificity changes and thereby facilitate the divergence in biological function.

-	~~==About this Structure==~~	+	Structural Basis for the Divergence of Substrate Specificity and Biological Function within HAD Phosphatases in Lipopolysaccharide and Sialic Acid Biosynthesis.,Daughtry KD, Huang H, Malashkevich V, Patskovsky Y, Liu W, Ramagopal U, Sauder JM, Burley SK, Almo SC, Dunaway-Mariano D, Allen KN Biochemistry. 2013 Aug 13;52(32):5372-86. doi: 10.1021/bi400659k. Epub 2013 Jul, 29. PMID:23848398<ref>PMID:23848398</ref>
-	~~[[4hgp]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Haemophilus_influenzae_rd_kw20 Haemophilus influenzae rd kw20]~~. ~~Full crystallographic information is available from [http~~:/~~/oca~~.~~weizmann~~.~~ac.il~~/~~oca-bin/ocashort?id=4HGP OCA].~~	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group="xtra">PMID:023848398</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: 3-deoxy-manno-octulosonate-8-phosphatase]]		[[Category: 3-deoxy-manno-octulosonate-8-phosphatase]]
	[[Category: Haemophilus influenzae rd kw20]]		[[Category: Haemophilus influenzae rd kw20]]
Line 14:		Line 27:
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Phosphohydroylase]]		[[Category: Phosphohydroylase]]
-	[[Category: ~~Rossman~~ fold]]	+	[[Category: Rossmann fold]]

OCA at 08:24, 21 August 2013

OCA — Wed, 21 Aug 2013 08:24:58 GMT

←Older revision		Revision as of 08:24, 21 August 2013
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{STRUCTURE_4hgp\| PDB=4hgp \| SCENE= }}
		+	===Crystal Structure of 2-keto-3-deoxyoctulosonate 8-phosphate phosphohydrolase from Haemophilus influenzae in complex with transition state mimic===
		+	{{ABSTRACT_PUBMED_23848398}}

-	~~The entry~~ 4hgp is ~~ON HOLD until Paper Publication~~	+	==About this Structure==
		+	[[4hgp]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Haemophilus_influenzae_rd_kw20 Haemophilus influenzae rd kw20]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HGP OCA].

-	~~Authors~~: ~~Daughtry, K.D., Allen, K.N.~~	+	==Reference==
-		+	<ref group="xtra">PMID:023848398</ref><references group="xtra"/><references/>
-	~~Description~~: ~~Crystal Structure of 2~~-~~keto~~-3-~~deoxyoctulosonate~~ 8-~~phosphate phosphohydrolase from~~ Haemophilus influenzae ~~in complex with transition state mimic~~	+	[[Category: 3-deoxy-manno-octulosonate-8-phosphatase]]
		+	[[Category: Haemophilus influenzae rd kw20]]
		+	[[Category: Allen, K N.]]
		+	[[Category: Daughtry, K D.]]
		+	[[Category: Hydrolase]]
		+	[[Category: Phosphohydroylase]]
		+	[[Category: Rossman fold]]

OCA at 13:21, 8 November 2012

OCA — Thu, 08 Nov 2012 13:21:48 GMT

←Older revision		Revision as of 13:21, 8 November 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4hgp is ON HOLD	+	The entry 4hgp is ON HOLD until Paper Publication

	Authors: Daughtry, K.D., Allen, K.N.		Authors: Daughtry, K.D., Allen, K.N.

	Description: Crystal Structure of 2-keto-3-deoxyoctulosonate 8-phosphate phosphohydrolase from Haemophilus influenzae in complex with transition state mimic		Description: Crystal Structure of 2-keto-3-deoxyoctulosonate 8-phosphate phosphohydrolase from Haemophilus influenzae in complex with transition state mimic

OCA: Protected "4hgp" [edit=sysop:move=sysop]

OCA — Wed, 17 Oct 2012 06:37:06 GMT

Protected "4hgp" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:37, 17 October 2012