4him - Revision history

OCA at 15:06, 20 September 2023

OCA — Wed, 20 Sep 2023 15:06:52 GMT

←Older revision		Revision as of 15:06, 20 September 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4him]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Schizosaccharomyces_pombe_972h- Schizosaccharomyces pombe 972h-]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HIM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4HIM FirstGlance]. <br>		<table><tr><td colspan='2'>[[4him]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Schizosaccharomyces_pombe_972h- Schizosaccharomyces pombe 972h-]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HIM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4HIM FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4him FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4him OCA], [https://pdbe.org/4him PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4him RCSB], [https://www.ebi.ac.uk/pdbsum/4him PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4him ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.75Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4him FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4him OCA], [https://pdbe.org/4him PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4him RCSB], [https://www.ebi.ac.uk/pdbsum/4him PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4him ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 08:19, 3 November 2022

OCA — Thu, 03 Nov 2022 08:19:12 GMT

←Older revision		Revision as of 08:19, 3 November 2022
Line 1:		Line 1:

	==Crystal Structure of Schizosaccharomyces pombe Pot1pC bound to ssDNA (GGATACGGT)==		==Crystal Structure of Schizosaccharomyces pombe Pot1pC bound to ssDNA (GGATACGGT)==
-	<StructureSection load='4him' size='340' side='right' caption='[[4him]], [[Resolution\|resolution]] 1.75Å' scene=''>	+	<StructureSection load='4him' size='340' side='right'caption='[[4him]], [[Resolution\|resolution]] 1.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4him]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Fission_yeast Fission yeast~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HIM OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4HIM FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4him]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Schizosaccharomyces_pombe_972h- Schizosaccharomyces pombe 972h-]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HIM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4HIM FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4hio\|4hio]], [[4hik\|4hik]], [[4hid\|4hid]], [[4hj5\|4hj5]], [[4hj7\|4hj7]], [[4hj8\|4hj8]], [[4hj9\|4hj9]], [[4hja\|4hja]]</td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4him FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4him OCA], [https://pdbe.org/4him PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4him RCSB], [https://www.ebi.ac.uk/pdbsum/4him PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4him ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pot1, SPAC26H5.06 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284812 Fission yeast])</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4him FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4him OCA], [~~http~~://pdbe.org/4him PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4him RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4him PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4him ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/POT1_SCHPO POT1_SCHPO]] Single-stranded telomeric DNA-binding protein that is required to protect the 3'-end telomeric overhang. It binds the consensus sequence 5'-GGTTAC-3'. Regulates telomerase and telomere length.<ref>PMID:11349150</ref> <ref>PMID:12463756</ref> <ref>PMID:18535244</ref>	+	[https://www.uniprot.org/uniprot/POT1_SCHPO POT1_SCHPO] Single-stranded telomeric DNA-binding protein that is required to protect the 3'-end telomeric overhang. It binds the consensus sequence 5'-GGTTAC-3'. Regulates telomerase and telomere length.<ref>PMID:11349150</ref> <ref>PMID:12463756</ref> <ref>PMID:18535244</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Fission yeast~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Dickey, T H]]~~	+	[[Category: Schizosaccharomyces pombe 972h-]]
-	~~[[Category: Wuttke, D S]]~~	+	[[Category: Dickey TH]]
-	~~[[Category: Dna binding protein]]~~	+	[[Category: Wuttke DS]]
-	~~[[Category: Ob~~-~~fold~~]]	+
-	[[Category: ~~Plasticity~~]]	+
-	[[Category: ~~Promiscuity]]~~	+
-	~~[[Category: Single-stranded telomeric dna]]~~	+
-	~~[[Category: Specificity]]~~	+
-	~~[[Category: Ssdna-binding~~]]	+

OCA at 20:18, 4 August 2016

OCA — Thu, 04 Aug 2016 20:18:25 GMT

←Older revision		Revision as of 20:18, 4 August 2016
Line 1:		Line 1:
		+
	==Crystal Structure of Schizosaccharomyces pombe Pot1pC bound to ssDNA (GGATACGGT)==		==Crystal Structure of Schizosaccharomyces pombe Pot1pC bound to ssDNA (GGATACGGT)==
	<StructureSection load='4him' size='340' side='right' caption='[[4him]], [[Resolution\|resolution]] 1.75Å' scene=''>		<StructureSection load='4him' size='340' side='right' caption='[[4him]], [[Resolution\|resolution]] 1.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4him]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Schizosaccharomyces_pombe_972h- Schizosaccharomyces pombe 972h-~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HIM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HIM FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4him]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Fission_yeast Fission yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HIM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HIM FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4hio\|4hio]], [[4hik\|4hik]], [[4hid\|4hid]], [[4hj5\|4hj5]], [[4hj7\|4hj7]], [[4hj8\|4hj8]], [[4hj9\|4hj9]], [[4hja\|4hja]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4hio\|4hio]], [[4hik\|4hik]], [[4hid\|4hid]], [[4hj5\|4hj5]], [[4hj7\|4hj7]], [[4hj8\|4hj8]], [[4hj9\|4hj9]], [[4hja\|4hja]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pot1, SPAC26H5.06 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284812 ~~Schizosaccharomyces pombe 972h-~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pot1, SPAC26H5.06 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284812 Fission yeast])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4him FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4him OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4him RCSB], [http://www.ebi.ac.uk/pdbsum/4him PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4him FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4him OCA], [http://pdbe.org/4him PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4him RCSB], [http://www.ebi.ac.uk/pdbsum/4him PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4him ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4him" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Schizosaccharomyces pombe 972h-~~]]	+	[[Category: Fission yeast]]
	[[Category: Dickey, T H]]		[[Category: Dickey, T H]]
	[[Category: Wuttke, D S]]		[[Category: Wuttke, D S]]

OCA at 19:14, 24 December 2014

OCA — Wed, 24 Dec 2014 19:14:43 GMT

←Older revision		Revision as of 19:14, 24 December 2014
Line 1:		Line 1:
-	[[~~Image:~~4him.~~png~~\|~~left~~\|~~200px~~]]	+	==Crystal Structure of Schizosaccharomyces pombe Pot1pC bound to ssDNA (GGATACGGT)==
		+	<StructureSection load='4him' size='340' side='right' caption='[[4him]], [[Resolution\|resolution]] 1.75Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4him]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Schizosaccharomyces_pombe_972h- Schizosaccharomyces pombe 972h-]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HIM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HIM FirstGlance]. <br>
		+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4hio\|4hio]], [[4hik\|4hik]], [[4hid\|4hid]], [[4hj5\|4hj5]], [[4hj7\|4hj7]], [[4hj8\|4hj8]], [[4hj9\|4hj9]], [[4hja\|4hja]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pot1, SPAC26H5.06 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284812 Schizosaccharomyces pombe 972h-])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4him FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4him OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4him RCSB], [http://www.ebi.ac.uk/pdbsum/4him PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/POT1_SCHPO POT1_SCHPO]] Single-stranded telomeric DNA-binding protein that is required to protect the 3'-end telomeric overhang. It binds the consensus sequence 5'-GGTTAC-3'. Regulates telomerase and telomere length.<ref>PMID:11349150</ref> <ref>PMID:12463756</ref> <ref>PMID:18535244</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Pot1 is the protein responsible for binding to and protecting the 3' single-stranded DNA (ssDNA) overhang at most eukaryotic telomeres. Here, we present the crystal structure of one of the two oligonucleotide/oligosaccharide-binding folds (Pot1pC) that make up the ssDNA-binding domain in S. pombe Pot1. Comparison with the homologous human domain reveals unexpected structural divergence in the mode of ligand binding that explains the differing ligand requirements between species. Despite the presence of apparently base-specific hydrogen bonds, Pot1pC is able to bind a wide range of ssDNA sequences with thermodynamic equivalence. To address how Pot1pC binds ssDNA with little to no specificity, multiple structures of Pot1pC bound to noncognate ssDNA ligands were solved. These structures reveal that this promiscuity is implemented through new binding modes that thermodynamically compensate for base-substitutions through alternate stacking interactions and new H-bonding networks.

-	~~{{STRUCTURE_4him\| PDB=4him \| SCENE= }}~~	+	Nonspecific Recognition Is Achieved in Pot1pC through the Use of Multiple Binding Modes.,Dickey TH, McKercher MA, Wuttke DS Structure. 2012 Nov 28. pii: S0969-2126(12)00411-X. doi:, 10.1016/j.str.2012.10.015. PMID:23201273<ref>PMID:23201273</ref>

-	~~===Crystal Structure~~ of ~~Schizosaccharomyces pombe Pot1pC bound to ssDNA (GGATACGGT)===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_23201273}}~~	+	== References ==
-		+	<references/>
-	==~~About this Structure~~==	+	__TOC__
-	~~[[4him]] is a 2 chain structure with sequence from [http:~~//~~en.wikipedia.org/wiki/Schizosaccharomyces_pombe_972h- Schizosaccharomyces pombe 972h-]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HIM OCA].~~	+	</StructureSection>
	[[Category: Schizosaccharomyces pombe 972h-]]		[[Category: Schizosaccharomyces pombe 972h-]]
-	[[Category: Dickey, T H.]]	+	[[Category: Dickey, T H]]
-	[[Category: Wuttke, D S.]]	+	[[Category: Wuttke, D S]]
	[[Category: Dna binding protein]]		[[Category: Dna binding protein]]
	[[Category: Ob-fold]]		[[Category: Ob-fold]]

OCA at 13:33, 27 February 2013

OCA — Wed, 27 Feb 2013 13:33:26 GMT

←Older revision		Revision as of 13:33, 27 February 2013
Line 1:		Line 1:
-	[[Image:4him.~~jpg~~\|left\|200px]]	+	[[Image:4him.png\|left\|200px]]

	{{STRUCTURE_4him\| PDB=4him \| SCENE= }}		{{STRUCTURE_4him\| PDB=4him \| SCENE= }}

OCA at 21:07, 12 December 2012

OCA — Wed, 12 Dec 2012 21:07:48 GMT

←Older revision		Revision as of 21:07, 12 December 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:4him.jpg\|left\|200px]]

-	~~The entry~~ 4him ~~is ON HOLD~~ ~~until Paper Publication~~	+	{{STRUCTURE_4him\| PDB=4him \| SCENE= }}

-	~~Authors: Dickey, T.D., Wuttke, D.S.~~	+	===Crystal Structure of Schizosaccharomyces pombe Pot1pC bound to ssDNA (GGATACGGT)===

-	~~Description: Crystal~~ Structure of Schizosaccharomyces pombe ~~Pot1pC bound to ssDNA (GGATACGGT)~~	+	{{ABSTRACT_PUBMED_23201273}}
		+
		+	==About this Structure==
		+	[[4him]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Schizosaccharomyces_pombe_972h- Schizosaccharomyces pombe 972h-]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HIM OCA].
		+	[[Category: Schizosaccharomyces pombe 972h-]]
		+	[[Category: Dickey, T H.]]
		+	[[Category: Wuttke, D S.]]
		+	[[Category: Dna binding protein]]
		+	[[Category: Ob-fold]]
		+	[[Category: Plasticity]]
		+	[[Category: Promiscuity]]
		+	[[Category: Single-stranded telomeric dna]]
		+	[[Category: Specificity]]
		+	[[Category: Ssdna-binding]]

OCA at 09:04, 21 November 2012

OCA — Wed, 21 Nov 2012 09:04:22 GMT

←Older revision		Revision as of 09:04, 21 November 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4him is ON HOLD	+	The entry 4him is ON HOLD until Paper Publication

	Authors: Dickey, T.D., Wuttke, D.S.		Authors: Dickey, T.D., Wuttke, D.S.

	Description: Crystal Structure of Schizosaccharomyces pombe Pot1pC bound to ssDNA (GGATACGGT)		Description: Crystal Structure of Schizosaccharomyces pombe Pot1pC bound to ssDNA (GGATACGGT)

OCA: Protected "4him" [edit=sysop:move=sysop]

OCA — Wed, 17 Oct 2012 06:39:03 GMT

Protected "4him" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:39, 17 October 2012

OCA: New page: '''Unreleased structure''' The entry 4him is ON HOLD Authors: Dickey, T.D., Wuttke, D.S. Description: Crystal Structure of Schizosaccharomyces pombe Pot1pC bound to ssDNA (GGATACGGT)

OCA — Wed, 17 Oct 2012 06:39:02 GMT

New page: '''Unreleased structure''' The entry 4him is ON HOLD Authors: Dickey, T.D., Wuttke, D.S. Description: Crystal Structure of Schizosaccharomyces pombe Pot1pC bound to ssDNA (GGATACGGT)

New page

'''Unreleased structure'''

The entry 4him is ON HOLD

Authors: Dickey, T.D., Wuttke, D.S.

Description: Crystal Structure of Schizosaccharomyces pombe Pot1pC bound to ssDNA (GGATACGGT)