4hjg - Revision history

OCA at 08:20, 3 November 2022

OCA — Thu, 03 Nov 2022 08:20:40 GMT

←Older revision		Revision as of 08:20, 3 November 2022
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	==Meditope-enabled trastuzumab==		==Meditope-enabled trastuzumab==
-	<StructureSection load='4hjg' size='340' side='right' caption='[[4hjg]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='4hjg' size='340' side='right'caption='[[4hjg]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4hjg]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"diplococcus_magnus"_prevot_1933 "diplococcus magnus" prevot 1933~~], [~~http~~://en.wikipedia.org/wiki/~~Human Human~~] and [~~http~~://en.wikipedia.org/wiki/~~Staam Staam~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HJG OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4HJG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4hjg]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Finegoldia_magna Finegoldia magna], [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_Mu50 Staphylococcus aureus subsp. aureus Mu50]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HJG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4HJG FirstGlance]. <br>
-	</td></tr>~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4hkz\|4hkz]], [[1n8z\|1n8z]]</td></tr>~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4hjg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hjg OCA], [https://pdbe.org/4hjg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4hjg RCSB], [https://www.ebi.ac.uk/pdbsum/4hjg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4hjg ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">spa, SAV0111 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=158878 STAAM])</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4hjg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hjg OCA], [~~http~~://pdbe.org/4hjg PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4hjg RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4hjg PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4hjg ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/SPA_STAAM SPA_STAAM]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 17:		Line 17:
	</div>		</div>
	<div class="pdbe-citations 4hjg" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4hjg" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Monoclonal Antibodies 3D structures\|Monoclonal Antibodies 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Diplococcus magnus prevot 1933~~]]	+	[[Category: Finegoldia magna]]
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
-	[[Category: ~~Staam~~]]	+	[[Category: Large Structures]]
-	[[Category: Avery~~, K N~~]]	+	[[Category: Staphylococcus aureus subsp. aureus Mu50]]
-	[[Category: Bzymek~~, K P~~]]	+	[[Category: Avery KN]]
-	[[Category: Donaldson~~, J M~~]]	+	[[Category: Bzymek KP]]
-	[[Category: Horne~~, D A~~]]	+	[[Category: Donaldson JM]]
-	[[Category: Williams~~, J C~~]]	+	[[Category: Horne DA]]
-	[[Category: Zer, C]]	+	[[Category: Williams JC]]
-	~~[[Category: Cancer therapeutic]]~~	+	[[Category: Zer C]]
-	~~[[Category: Immune system]]~~	+
-	~~[[Category: Monoclonal antibody~~]]	+

OCA at 22:05, 5 August 2016

OCA — Fri, 05 Aug 2016 22:05:31 GMT

←Older revision		Revision as of 22:05, 5 August 2016
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		+
	==Meditope-enabled trastuzumab==		==Meditope-enabled trastuzumab==
	<StructureSection load='4hjg' size='340' side='right' caption='[[4hjg]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='4hjg' size='340' side='right' caption='[[4hjg]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4hjg]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Finegoldia_magna Finegoldia magna~~], [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~] and [http://en.wikipedia.org/wiki/~~Staphylococcus_aureus_subsp._aureus_mu50 Staphylococcus aureus subsp. aureus mu50~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HJG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HJG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4hjg]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"diplococcus_magnus"_prevot_1933 "diplococcus magnus" prevot 1933], [http://en.wikipedia.org/wiki/Human Human] and [http://en.wikipedia.org/wiki/Staam Staam]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HJG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HJG FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4hkz\|4hkz]], [[1n8z\|1n8z]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4hkz\|4hkz]], [[1n8z\|1n8z]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">spa, SAV0111 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=158878 ~~Staphylococcus aureus subsp. aureus Mu50~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">spa, SAV0111 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=158878 STAAM])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hjg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hjg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4hjg RCSB], [http://www.ebi.ac.uk/pdbsum/4hjg PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hjg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hjg OCA], [http://pdbe.org/4hjg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4hjg RCSB], [http://www.ebi.ac.uk/pdbsum/4hjg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4hjg ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 15:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4hjg" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Finegoldia magna~~]]	+	[[Category: Diplococcus magnus prevot 1933]]
-	[[Category: ~~Homo sapiens~~]]	+	[[Category: Human]]
-	[[Category: ~~Staphylococcus aureus subsp. aureus mu50~~]]	+	[[Category: Staam]]
	[[Category: Avery, K N]]		[[Category: Avery, K N]]
	[[Category: Bzymek, K P]]		[[Category: Bzymek, K P]]

OCA at 13:07, 21 December 2014

OCA — Sun, 21 Dec 2014 13:07:26 GMT

←Older revision		Revision as of 13:07, 21 December 2014
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-	~~{{STRUCTURE_4hjg~~\| ~~PDB~~=4hjg \| ~~SCENE~~= }}	+	==Meditope-enabled trastuzumab==
-	===~~Meditope~~-~~enabled trastuzumab~~===	+	<StructureSection load='4hjg' size='340' side='right' caption='[[4hjg]], [[Resolution\|resolution]] 2.00Å' scene=''>
-	~~{{ABSTRACT_PUBMED_24101516}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4hjg]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Finegoldia_magna Finegoldia magna], [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_mu50 Staphylococcus aureus subsp. aureus mu50]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HJG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HJG FirstGlance]. <br>
		+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4hkz\|4hkz]], [[1n8z\|1n8z]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">spa, SAV0111 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=158878 Staphylococcus aureus subsp. aureus Mu50])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hjg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hjg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4hjg RCSB], [http://www.ebi.ac.uk/pdbsum/4hjg PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Capitalizing on their extraordinary specificity, monoclonal antibodies (mAbs) have become one of the most reengineered classes of biological molecules. A major goal in many of these engineering efforts is to add new functionality to the parental mAb, including the addition of cytotoxins and imaging agents for medical applications. Herein, we present a unique peptide-binding site within the central cavity of the fragment antigen binding framework region of the chimeric, anti-epidermal growth factor receptor mAb cetuximab. We demonstrate through diffraction methods, biophysical studies, and sequence analysis that this peptide, a meditope, has moderate affinity for the Fab, is specific to cetuximab (i.e., does not bind to human IgGs), and has no significant effect on antigen binding. We further demonstrate by diffraction studies and biophysical methods that the meditope binding site can be grafted onto the anti-human epidermal growth factor receptor 2 mAb trastuzumab, and that the antigen binding affinity of the grafted trastuzumab is indistinguishable from the parental mAb. Finally, we demonstrate a bivalent meditope variant binds specifically and stably to antigen-bearing cells only in the presence of the meditope-enabled mAbs. Collectively, this finding and the subsequent characterization and engineering efforts indicate that this unique interface could serve as a noncovalent "linker" for any meditope-enabled mAb with applications in multiple mAb-based technologies including diagnostics, imaging, and therapeutic delivery.

-	~~==About this Structure==~~	+	Identification and grafting of a unique peptide-binding site in the Fab framework of monoclonal antibodies.,Donaldson JM, Zer C, Avery KN, Bzymek KP, Horne DA, Williams JC Proc Natl Acad Sci U S A. 2013 Oct 7. PMID:24101516<ref>PMID:24101516</ref>
-	~~[[4hjg]] is~~ a ~~4 chain structure with sequence from [http://en~~.~~wikipedia.org/wiki/Finegoldia_magna Finegoldia magna]~~, ~~[http://en~~.~~wikipedia~~.~~org/wiki/Homo_sapiens Homo sapiens] and [http~~:~~//en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_mu50 Staphylococcus aureus subsp. aureus mu50]. Full crystallographic information is available from [http~~:/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=4HJG OCA].~~	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group="xtra">PMID:024101516</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Finegoldia magna]]		[[Category: Finegoldia magna]]
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Staphylococcus aureus subsp. aureus mu50]]		[[Category: Staphylococcus aureus subsp. aureus mu50]]
-	[[Category: Avery, K N.]]	+	[[Category: Avery, K N]]
-	[[Category: Bzymek, K P.]]	+	[[Category: Bzymek, K P]]
-	[[Category: Donaldson, J M.]]	+	[[Category: Donaldson, J M]]
-	[[Category: Horne, D A.]]	+	[[Category: Horne, D A]]
-	[[Category: Williams, J C.]]	+	[[Category: Williams, J C]]
-	[[Category: Zer, C.]]	+	[[Category: Zer, C]]
	[[Category: Cancer therapeutic]]		[[Category: Cancer therapeutic]]
	[[Category: Immune system]]		[[Category: Immune system]]
	[[Category: Monoclonal antibody]]		[[Category: Monoclonal antibody]]

OCA at 05:16, 23 October 2013

OCA — Wed, 23 Oct 2013 05:16:45 GMT

←Older revision		Revision as of 05:16, 23 October 2013
Line 1:		Line 1:
	{{STRUCTURE_4hjg\| PDB=4hjg \| SCENE= }}		{{STRUCTURE_4hjg\| PDB=4hjg \| SCENE= }}
	===Meditope-enabled trastuzumab===		===Meditope-enabled trastuzumab===
		+	{{ABSTRACT_PUBMED_24101516}}

	==About this Structure==		==About this Structure==
	[[4hjg]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Finegoldia_magna Finegoldia magna], [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_mu50 Staphylococcus aureus subsp. aureus mu50]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HJG OCA].		[[4hjg]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Finegoldia_magna Finegoldia magna], [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_mu50 Staphylococcus aureus subsp. aureus mu50]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HJG OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:024101516</ref><references group="xtra"/><references/>
	[[Category: Finegoldia magna]]		[[Category: Finegoldia magna]]
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]

OCA at 06:37, 10 October 2013

OCA — Thu, 10 Oct 2013 06:37:53 GMT

←Older revision		Revision as of 06:37, 10 October 2013
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{STRUCTURE_4hjg\| PDB=4hjg \| SCENE= }}
		+	===Meditope-enabled trastuzumab===

-	~~The entry~~ 4hjg is ~~ON HOLD until Apr 11 2015~~	+	==About this Structure==
-		+	[[4hjg]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Finegoldia_magna Finegoldia magna], [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_mu50 Staphylococcus aureus subsp. aureus mu50]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HJG OCA].
-	~~Authors~~: ~~Donaldson, J~~.M., ~~Zer, C~~., Avery, K.N., Bzymek, K.P., Horne, D.A., Williams, J.C.	+	[[Category: Finegoldia magna]]
-		+	[[Category: Homo sapiens]]
-	~~Description~~: ~~Meditope-enabled trastuzumab~~	+	[[Category: Staphylococcus aureus subsp. aureus mu50]]
		+	[[Category: Avery, K N.]]
		+	[[Category: Bzymek, K P.]]
		+	[[Category: Donaldson, J M.]]
		+	[[Category: Horne, D A.]]
		+	[[Category: Williams, J C.]]
		+	[[Category: Zer, C.]]
		+	[[Category: Cancer therapeutic]]
		+	[[Category: Immune system]]
		+	[[Category: Monoclonal antibody]]

OCA at 12:07, 24 July 2013

OCA — Wed, 24 Jul 2013 12:07:57 GMT

←Older revision		Revision as of 12:07, 24 July 2013
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4hjg is ON HOLD until ~~Paper Publication~~	+	The entry 4hjg is ON HOLD until Apr 11 2015

	Authors: Donaldson, J.M., Zer, C., Avery, K.N., Bzymek, K.P., Horne, D.A., Williams, J.C.		Authors: Donaldson, J.M., Zer, C., Avery, K.N., Bzymek, K.P., Horne, D.A., Williams, J.C.

	Description: Meditope-enabled trastuzumab		Description: Meditope-enabled trastuzumab

OCA: Protected "4hjg" [edit=sysop:move=sysop]

OCA — Wed, 24 Oct 2012 07:11:57 GMT

Protected "4hjg" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:11, 24 October 2012

OCA: New page: '''Unreleased structure''' The entry 4hjg is ON HOLD until Paper Publication Authors: Donaldson, J.M., Zer, C., Avery, K.N., Bzymek, K.P., Horne, D.A., Williams, J.C. Description: Medi...

OCA — Wed, 24 Oct 2012 07:11:56 GMT

New page: '''Unreleased structure''' The entry 4hjg is ON HOLD until Paper Publication Authors: Donaldson, J.M., Zer, C., Avery, K.N., Bzymek, K.P., Horne, D.A., Williams, J.C. Description: Medi...

New page

'''Unreleased structure'''

The entry 4hjg is ON HOLD until Paper Publication

Authors: Donaldson, J.M., Zer, C., Avery, K.N., Bzymek, K.P., Horne, D.A., Williams, J.C.

Description: Meditope-enabled trastuzumab