4hnu - Revision history

OCA at 11:42, 1 March 2024

OCA — Fri, 01 Mar 2024 11:42:35 GMT

←Older revision		Revision as of 11:42, 1 March 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4hnu]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HNU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4HNU FirstGlance]. <br>		<table><tr><td colspan='2'>[[4hnu]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HNU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4HNU FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=BTI:5-(HEXAHYDRO-2-OXO-1H-THIENO[3,4-D]IMIDAZOL-6-YL)PENTANAL'>BTI</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=BTI:5-(HEXAHYDRO-2-OXO-1H-THIENO[3,4-D]IMIDAZOL-6-YL)PENTANAL'>BTI</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4hnu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hnu OCA], [https://pdbe.org/4hnu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4hnu RCSB], [https://www.ebi.ac.uk/pdbsum/4hnu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4hnu ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4hnu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hnu OCA], [https://pdbe.org/4hnu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4hnu RCSB], [https://www.ebi.ac.uk/pdbsum/4hnu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4hnu ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/PYC_STAAM PYC_STAAM] Catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second.<ref>PMID:19523900</ref> <ref>PMID:23286247</ref>		[https://www.uniprot.org/uniprot/PYC_STAAM PYC_STAAM] Catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second.<ref>PMID:19523900</ref> <ref>PMID:23286247</ref>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Biotin carboxylase (BC) is a conserved component among biotin-dependent carboxylases and catalyzes the MgATP-dependent carboxylation of biotin, using bicarbonate as the CO(2) donor. Studies with Escherichia coli BC have suggested long-range communication between the two active sites of a dimer, although its mechanism is not well understood. In addition, mutations in the dimer interface can produce stable monomers that are still catalytically active. A homologous dimer for the BC domain is observed in the structure of the tetrameric pyruvate carboxylase (PC) holoenzyme. We have introduced site-specific mutations into the BC domain dimer interface of Staphylococcus aureus PC (SaPC), equivalent to those used for E. coli BC, and also made chimeras replacing the SaPC BC domain with the E. coli BC subunit (EcBC chimera) or the yeast ACC BC domain (ScBC chimera). We assessed the catalytic activities of these mutants and characterized their oligomerization states by gel filtration and analytical ultracentrifugation experiments. The K442E mutant and the ScBC chimera disrupted the BC dimer and were catalytically inactive, while the F403A mutant and the EcBC chimera were still tetrameric and retained catalytic activity. The R54E mutant was also tetrameric but was catalytically inactive. Crystal structures of the R54E, F403A, and K442E mutants showed that they were tetrameric in the crystal, with conformational changes near the mutation site as well as in the tetramer organization. We have also produced the isolated BC domain of SaPC. In contrast to E. coli BC, the SaPC BC domain is monomeric in solution and catalytically inactive.

-	Characterizing the Importance of the Biotin Carboxylase Domain Dimer for Staphylococcus aureus Pyruvate Carboxylase Catalysis.,Yu LP, Chou CY, Choi PH, Tong L Biochemistry. 2013 Jan 9. PMID:23286247<ref>PMID:23286247</ref>	+	==See Also==
-		+	*[[Pyruvate carboxylase 3D structures\|Pyruvate carboxylase 3D structures]]
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class~~=~~"pdbe-citations 4hnu" style~~=~~"background-color:#fffaf0;"></div>~~	+
	== References ==		== References ==
	<references/>		<references/>

OCA at 08:28, 3 November 2022

OCA — Thu, 03 Nov 2022 08:28:53 GMT

←Older revision		Revision as of 08:28, 3 November 2022
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	<StructureSection load='4hnu' size='340' side='right'caption='[[4hnu]], [[Resolution\|resolution]] 3.00Å' scene=''>		<StructureSection load='4hnu' size='340' side='right'caption='[[4hnu]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4hnu]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus~~ aureus~~" (rosenbach 1884) zopf 1885~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HNU OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4HNU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4hnu]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HNU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4HNU FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=BTI:5-(HEXAHYDRO-2-OXO-1H-THIENO[3,4-D]IMIDAZOL-6-YL)PENTANAL'>BTI</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene~~></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=BTI:5-(HEXAHYDRO-2-OXO-1H-THIENO[3,4-D]IMIDAZOL-6-YL)PENTANAL'>BTI</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3bg5\|3bg5]], [[4hnt\|4hnt]], [[4hnv\|4hnv]]</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4hnu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hnu OCA], [https://pdbe.org/4hnu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4hnu RCSB], [https://www.ebi.ac.uk/pdbsum/4hnu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4hnu ProSAT]</span></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pycA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 "Micrococcus aureus" (Rosenbach 1884) Zopf 1885])</td></tr>	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Pyruvate_carboxylase Pyruvate carboxylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.4.1.1 6.4.1.1] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4hnu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hnu OCA], [~~http~~://pdbe.org/4hnu PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4hnu RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4hnu PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4hnu ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/~~Q99UY8_STAAM Q99UY8_STAAM]~~] Catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second ~~(By similarity)~~.~~[PIRNR~~:~~PIRNR001594]~~	+	[https://www.uniprot.org/uniprot/PYC_STAAM PYC_STAAM] Catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second.<ref>PMID:19523900</ref> <ref>PMID:23286247</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 26:		Line 23:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Pyruvate carboxylase~~]]	+	[[Category: Staphylococcus aureus]]
-	[[Category: Tong, L]]	+	[[Category: Tong L]]
-	[[Category: Yu~~, L P.C]]~~	+	[[Category: Yu LPC]]
-	~~[[Category: Ligase~~]]	+

OCA at 11:57, 17 July 2019

OCA — Wed, 17 Jul 2019 11:57:36 GMT

←Older revision		Revision as of 11:57, 17 July 2019
Line 1:		Line 1:

	==crystal structure of K442E mutant of S. aureus Pyruvate carboxylase==		==crystal structure of K442E mutant of S. aureus Pyruvate carboxylase==
-	<StructureSection load='4hnu' size='340' side='right' caption='[[4hnu]], [[Resolution\|resolution]] 3.00Å' scene=''>	+	<StructureSection load='4hnu' size='340' side='right'caption='[[4hnu]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4hnu]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HNU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HNU FirstGlance]. <br>		<table><tr><td colspan='2'>[[4hnu]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HNU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HNU FirstGlance]. <br>
Line 25:		Line 25:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Pyruvate carboxylase]]		[[Category: Pyruvate carboxylase]]
	[[Category: Tong, L]]		[[Category: Tong, L]]
	[[Category: Yu, L P.C]]		[[Category: Yu, L P.C]]
	[[Category: Ligase]]		[[Category: Ligase]]

OCA at 07:25, 5 August 2016

OCA — Fri, 05 Aug 2016 07:25:52 GMT

←Older revision		Revision as of 07:25, 5 August 2016
Line 1:		Line 1:
		+
	==crystal structure of K442E mutant of S. aureus Pyruvate carboxylase==		==crystal structure of K442E mutant of S. aureus Pyruvate carboxylase==
	<StructureSection load='4hnu' size='340' side='right' caption='[[4hnu]], [[Resolution\|resolution]] 3.00Å' scene=''>		<StructureSection load='4hnu' size='340' side='right' caption='[[4hnu]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4hnu]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Staphylococcus_aureus Staphylococcus~~ aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HNU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HNU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4hnu]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HNU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HNU FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=BTI:5-(HEXAHYDRO-2-OXO-1H-THIENO[3,4-D]IMIDAZOL-6-YL)PENTANAL'>BTI</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=BTI:5-(HEXAHYDRO-2-OXO-1H-THIENO[3,4-D]IMIDAZOL-6-YL)PENTANAL'>BTI</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3bg5\|3bg5]], [[4hnt\|4hnt]], [[4hnv\|4hnv]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3bg5\|3bg5]], [[4hnt\|4hnt]], [[4hnv\|4hnv]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pycA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 ~~Staphylococcus~~ aureus])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pycA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 "Micrococcus aureus" (Rosenbach 1884) Zopf 1885])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Pyruvate_carboxylase Pyruvate carboxylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.4.1.1 6.4.1.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Pyruvate_carboxylase Pyruvate carboxylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.4.1.1 6.4.1.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hnu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hnu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4hnu RCSB], [http://www.ebi.ac.uk/pdbsum/4hnu PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hnu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hnu OCA], [http://pdbe.org/4hnu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4hnu RCSB], [http://www.ebi.ac.uk/pdbsum/4hnu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4hnu ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 19:		Line 20:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4hnu" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
Line 24:		Line 26:
	</StructureSection>		</StructureSection>
	[[Category: Pyruvate carboxylase]]		[[Category: Pyruvate carboxylase]]
-	[[Category: Staphylococcus aureus]]
	[[Category: Tong, L]]		[[Category: Tong, L]]
	[[Category: Yu, L P.C]]		[[Category: Yu, L P.C]]
	[[Category: Ligase]]		[[Category: Ligase]]

OCA at 00:14, 25 December 2014

OCA — Thu, 25 Dec 2014 00:14:50 GMT

←Older revision		Revision as of 00:14, 25 December 2014
Line 9:		Line 9:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hnu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hnu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4hnu RCSB], [http://www.ebi.ac.uk/pdbsum/4hnu PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hnu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hnu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4hnu RCSB], [http://www.ebi.ac.uk/pdbsum/4hnu PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/Q99UY8_STAAM Q99UY8_STAAM]] Catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second (By similarity).[PIRNR:PIRNR001594]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 09:18, 10 December 2014

OCA — Wed, 10 Dec 2014 09:18:09 GMT

←Older revision		Revision as of 09:18, 10 December 2014
Line 1:		Line 1:
-	[[~~Image:~~4hnu.~~png~~\|~~left~~\|~~200px~~]]	+	==crystal structure of K442E mutant of S. aureus Pyruvate carboxylase==
		+	<StructureSection load='4hnu' size='340' side='right' caption='[[4hnu]], [[Resolution\|resolution]] 3.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4hnu]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HNU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HNU FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=BTI:5-(HEXAHYDRO-2-OXO-1H-THIENO[3,4-D]IMIDAZOL-6-YL)PENTANAL'>BTI</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3bg5\|3bg5]], [[4hnt\|4hnt]], [[4hnv\|4hnv]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pycA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 Staphylococcus aureus])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Pyruvate_carboxylase Pyruvate carboxylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.4.1.1 6.4.1.1] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hnu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hnu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4hnu RCSB], [http://www.ebi.ac.uk/pdbsum/4hnu PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Biotin carboxylase (BC) is a conserved component among biotin-dependent carboxylases and catalyzes the MgATP-dependent carboxylation of biotin, using bicarbonate as the CO(2) donor. Studies with Escherichia coli BC have suggested long-range communication between the two active sites of a dimer, although its mechanism is not well understood. In addition, mutations in the dimer interface can produce stable monomers that are still catalytically active. A homologous dimer for the BC domain is observed in the structure of the tetrameric pyruvate carboxylase (PC) holoenzyme. We have introduced site-specific mutations into the BC domain dimer interface of Staphylococcus aureus PC (SaPC), equivalent to those used for E. coli BC, and also made chimeras replacing the SaPC BC domain with the E. coli BC subunit (EcBC chimera) or the yeast ACC BC domain (ScBC chimera). We assessed the catalytic activities of these mutants and characterized their oligomerization states by gel filtration and analytical ultracentrifugation experiments. The K442E mutant and the ScBC chimera disrupted the BC dimer and were catalytically inactive, while the F403A mutant and the EcBC chimera were still tetrameric and retained catalytic activity. The R54E mutant was also tetrameric but was catalytically inactive. Crystal structures of the R54E, F403A, and K442E mutants showed that they were tetrameric in the crystal, with conformational changes near the mutation site as well as in the tetramer organization. We have also produced the isolated BC domain of SaPC. In contrast to E. coli BC, the SaPC BC domain is monomeric in solution and catalytically inactive.

-	~~{{STRUCTURE_4hnu\| PDB=4hnu \| SCENE= }}~~	+	Characterizing the Importance of the Biotin Carboxylase Domain Dimer for Staphylococcus aureus Pyruvate Carboxylase Catalysis.,Yu LP, Chou CY, Choi PH, Tong L Biochemistry. 2013 Jan 9. PMID:23286247<ref>PMID:23286247</ref>

-	~~===crystal structure of K442E mutant~~ of S. ~~aureus Pyruvate carboxylase===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_23286247}}~~	+	== References ==
-		+	<references/>
-	==~~About this Structure~~==	+	__TOC__
-	~~[[4hnu]] is a 4 chain structure with sequence from [http:~~//~~en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HNU OCA].~~	+	</StructureSection>
	[[Category: Pyruvate carboxylase]]		[[Category: Pyruvate carboxylase]]
	[[Category: Staphylococcus aureus]]		[[Category: Staphylococcus aureus]]
-	[[Category: Tong, L.]]	+	[[Category: Tong, L]]
-	[[Category: Yu, L P.C.]]	+	[[Category: Yu, L P.C]]
	[[Category: Ligase]]		[[Category: Ligase]]

OCA at 12:48, 6 March 2013

OCA — Wed, 06 Mar 2013 12:48:31 GMT

←Older revision		Revision as of 12:48, 6 March 2013
Line 1:		Line 1:
-	[[Image:4hnu.~~jpg~~\|left\|200px]]	+	[[Image:4hnu.png\|left\|200px]]

	{{STRUCTURE_4hnu\| PDB=4hnu \| SCENE= }}		{{STRUCTURE_4hnu\| PDB=4hnu \| SCENE= }}

OCA at 08:48, 16 January 2013

OCA — Wed, 16 Jan 2013 08:48:44 GMT

←Older revision		Revision as of 08:48, 16 January 2013
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:4hnu.jpg\|left\|200px]]

-	~~The entry~~ 4hnu ~~is ON HOLD~~ ~~until Paper Publication~~	+	{{STRUCTURE_4hnu\| PDB=4hnu \| SCENE= }}

-	~~Authors: Yu, L.P.C., Tong, L~~.	+	===crystal structure of K442E mutant of S. aureus Pyruvate carboxylase===

-	~~Description: crystal~~ structure ~~of K442E mutant of S~~. aureus Pyruvate carboxylase	+	{{ABSTRACT_PUBMED_23286247}}
		+
		+	==About this Structure==
		+	[[4hnu]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HNU OCA].
		+	[[Category: Pyruvate carboxylase]]
		+	[[Category: Staphylococcus aureus]]
		+	[[Category: Tong, L.]]
		+	[[Category: Yu, L P.C.]]
		+	[[Category: Ligase]]

OCA at 06:16, 14 November 2012

OCA — Wed, 14 Nov 2012 06:16:31 GMT

←Older revision		Revision as of 06:16, 14 November 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4hnu is ON HOLD	+	The entry 4hnu is ON HOLD until Paper Publication

	Authors: Yu, L.P.C., Tong, L.		Authors: Yu, L.P.C., Tong, L.

	Description: crystal structure of K442E mutant of S. aureus Pyruvate carboxylase		Description: crystal structure of K442E mutant of S. aureus Pyruvate carboxylase

OCA: Protected "4hnu" [edit=sysop:move=sysop]

OCA — Wed, 31 Oct 2012 08:41:49 GMT

Protected "4hnu" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:41, 31 October 2012