4hpf - Revision history

OCA at 15:09, 20 September 2023

OCA — Wed, 20 Sep 2023 15:09:23 GMT

←Older revision		Revision as of 15:09, 20 September 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[4hpf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HPF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4HPF FirstGlance]. <br>		<table><tr><td colspan='2'>[[4hpf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HPF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4HPF FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4hpf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hpf OCA], [https://pdbe.org/4hpf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4hpf RCSB], [https://www.ebi.ac.uk/pdbsum/4hpf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4hpf ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.4Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4hpf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hpf OCA], [https://pdbe.org/4hpf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4hpf RCSB], [https://www.ebi.ac.uk/pdbsum/4hpf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4hpf ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 08:31, 3 November 2022

OCA — Thu, 03 Nov 2022 08:31:00 GMT

←Older revision		Revision as of 08:31, 3 November 2022
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	==Structure of the human SLO3 gating ring==		==Structure of the human SLO3 gating ring==
-	<StructureSection load='4hpf' size='340' side='right' caption='[[4hpf]], [[Resolution\|resolution]] 3.40Å' scene=''>	+	<StructureSection load='4hpf' size='340' side='right'caption='[[4hpf]], [[Resolution\|resolution]] 3.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4hpf]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HPF OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4HPF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4hpf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HPF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4HPF FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">KCNMA3, KCNMC1, KCNU1, SLO3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4hpf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hpf OCA], [https://pdbe.org/4hpf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4hpf RCSB], [https://www.ebi.ac.uk/pdbsum/4hpf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4hpf ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4hpf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hpf OCA], [~~http~~://pdbe.org/4hpf PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4hpf RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4hpf PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4hpf ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/KCNU1_HUMAN KCNU1_HUMAN]] Testis-specific potassium channel activated by both intracellular pH and membrane voltage that mediates export of K(+). May represent the primary spermatozoan K(+) current. In contrast to KCNMA1/SLO1, it is not activated by Ca(2+) or Mg(2+). Critical for fertility. May play an important role in sperm osmoregulation required for the acquisition of normal morphology and motility when faced with osmotic challenges, such as those experienced after mixing with seminal fluid and entry into the vagina.<ref>PMID:23129643</ref>	+	[https://www.uniprot.org/uniprot/KCNU1_HUMAN KCNU1_HUMAN] Testis-specific potassium channel activated by both intracellular pH and membrane voltage that mediates export of K(+). May represent the primary spermatozoan K(+) current. In contrast to KCNMA1/SLO1, it is not activated by Ca(2+) or Mg(2+). Critical for fertility. May play an important role in sperm osmoregulation required for the acquisition of normal morphology and motility when faced with osmotic challenges, such as those experienced after mixing with seminal fluid and entry into the vagina.<ref>PMID:23129643</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 4hpf" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4hpf" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Potassium channel 3D structures\|Potassium channel 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
-	[[Category: ~~Leonetti, M D~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~MacKinnon, R~~]]	+	[[Category: Leonetti MD]]
-	[[Category: ~~Yuan, P~~]]	+	[[Category: MacKinnon R]]
-	[[Category: ~~Membrane protein]]~~	+	[[Category: Yuan P]]
-	~~[[Category: Ph-gated]]~~	+
-	~~[[Category: Potassium channel]]~~	+
-	~~[[Category: Transport protein~~]]	+

OCA at 08:43, 15 November 2017

OCA — Wed, 15 Nov 2017 08:43:41 GMT

←Older revision		Revision as of 08:43, 15 November 2017
Line 18:		Line 18:
	</div>		</div>
	<div class="pdbe-citations 4hpf" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 4hpf" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Potassium Channel\|Potassium Channel]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 05:07, 5 August 2016

OCA — Fri, 05 Aug 2016 05:07:40 GMT

←Older revision		Revision as of 05:07, 5 August 2016
Line 1:		Line 1:
		+
	==Structure of the human SLO3 gating ring==		==Structure of the human SLO3 gating ring==
	<StructureSection load='4hpf' size='340' side='right' caption='[[4hpf]], [[Resolution\|resolution]] 3.40Å' scene=''>		<StructureSection load='4hpf' size='340' side='right' caption='[[4hpf]], [[Resolution\|resolution]] 3.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4hpf]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HPF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HPF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4hpf]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HPF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HPF FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">KCNMA3, KCNMC1, KCNU1, SLO3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 ~~Homo sapiens~~])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">KCNMA3, KCNMC1, KCNU1, SLO3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hpf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hpf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4hpf RCSB], [http://www.ebi.ac.uk/pdbsum/4hpf PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hpf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hpf OCA], [http://pdbe.org/4hpf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4hpf RCSB], [http://www.ebi.ac.uk/pdbsum/4hpf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4hpf ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4hpf" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Potassium Channel\|Potassium Channel]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Homo sapiens~~]]	+	[[Category: Human]]
	[[Category: Leonetti, M D]]		[[Category: Leonetti, M D]]
	[[Category: MacKinnon, R]]		[[Category: MacKinnon, R]]

OCA at 16:00, 25 December 2014

OCA — Thu, 25 Dec 2014 16:00:22 GMT

←Older revision		Revision as of 16:00, 25 December 2014
Line 6:		Line 6:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hpf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hpf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4hpf RCSB], [http://www.ebi.ac.uk/pdbsum/4hpf PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hpf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hpf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4hpf RCSB], [http://www.ebi.ac.uk/pdbsum/4hpf PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/KCNU1_HUMAN KCNU1_HUMAN]] Testis-specific potassium channel activated by both intracellular pH and membrane voltage that mediates export of K(+). May represent the primary spermatozoan K(+) current. In contrast to KCNMA1/SLO1, it is not activated by Ca(2+) or Mg(2+). Critical for fertility. May play an important role in sperm osmoregulation required for the acquisition of normal morphology and motility when faced with osmotic challenges, such as those experienced after mixing with seminal fluid and entry into the vagina.<ref>PMID:23129643</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 09:49, 10 December 2014

OCA — Wed, 10 Dec 2014 09:49:41 GMT

←Older revision		Revision as of 09:49, 10 December 2014
Line 1:		Line 1:
-	[[~~Image:~~4hpf.~~png~~\|~~left\|200px~~]]	+	==Structure of the human SLO3 gating ring==
		+	<StructureSection load='4hpf' size='340' side='right' caption='[[4hpf]], [[Resolution\|resolution]] 3.40Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4hpf]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HPF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HPF FirstGlance]. <br>
		+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">KCNMA3, KCNMC1, KCNU1, SLO3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hpf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hpf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4hpf RCSB], [http://www.ebi.ac.uk/pdbsum/4hpf PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The activation of eukaryotic SLO K(+) channels by intracellular cues, mediated by a cytoplasmic structure called the gating ring, is central to their physiological roles. SLO3 channels are exclusively expressed in mammalian sperm, where variations of intracellular pH are critical to cellular function. Previous studies primarily focused on the mouse SLO3 orthologue and revealed that, in murine sperm, SLO3 mediates a voltage- and alkalization-activated K(+) current essential to male fertility. Here we investigate the activation of the human SLO3 channel by intracellular pH at the functional and structural level. By using electrophysiology in a heterologous system, we show that human SLO3 opens upon intracellular pH increase and that its expression and functional properties are modulated by LRRC52, a testis-specific accessory subunit. We next present the crystal structure of the human SLO3 gating ring. Comparison with the known structures of the corresponding domain from SLO1, a Ca(2+)-activated homologue, suggests that the SLO3 gating ring structure may represent an open state. Together, these results present insights into the function of a protein expected to be critical for human reproduction and provide a framework to study the mechanism of pH gating in SLO3 channels.

-	~~{{STRUCTURE_4hpf\| PDB=4hpf \| SCENE= }}~~	+	Functional and structural analysis of the human SLO3 pH- and voltage-gated K+ channel.,Leonetti MD, Yuan P, Hsiung Y, Mackinnon R Proc Natl Acad Sci U S A. 2012 Nov 20;109(47):19274-9. doi:, 10.1073/pnas.1215078109. Epub 2012 Nov 5. PMID:23129643<ref>PMID:23129643</ref>

-	~~===Structure~~ of the ~~human SLO3 gating ring===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_23129643}}~~	+	== References ==
-		+	<references/>
-	==~~About this Structure~~==	+	__TOC__
-	~~[[4hpf]] is a 2 chain structure with sequence from [http:~~//~~en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HPF OCA].~~	+	</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
-	[[Category: Leonetti, M D.]]	+	[[Category: Leonetti, M D]]
-	[[Category: MacKinnon, R.]]	+	[[Category: MacKinnon, R]]
-	[[Category: Yuan, P.]]	+	[[Category: Yuan, P]]
	[[Category: Membrane protein]]		[[Category: Membrane protein]]
	[[Category: Ph-gated]]		[[Category: Ph-gated]]
	[[Category: Potassium channel]]		[[Category: Potassium channel]]
	[[Category: Transport protein]]		[[Category: Transport protein]]

OCA at 09:58, 5 December 2012

OCA — Wed, 05 Dec 2012 09:58:52 GMT

←Older revision		Revision as of 09:58, 5 December 2012
Line 1:		Line 1:
-	[[Image:4hpf.~~jpg~~\|left\|200px]]	+	[[Image:4hpf.png\|left\|200px]]

	{{STRUCTURE_4hpf\| PDB=4hpf \| SCENE= }}		{{STRUCTURE_4hpf\| PDB=4hpf \| SCENE= }}
Line 5:		Line 5:
	===Structure of the human SLO3 gating ring===		===Structure of the human SLO3 gating ring===

		+	{{ABSTRACT_PUBMED_23129643}}

	==About this Structure==		==About this Structure==

OCA at 14:06, 8 November 2012

OCA — Thu, 08 Nov 2012 14:06:30 GMT

←Older revision		Revision as of 14:06, 8 November 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:4hpf.jpg\|left\|200px]]

-	~~The entry~~ 4hpf ~~is ON HOLD~~	+	{{STRUCTURE_4hpf\| PDB=4hpf \| SCENE= }}

-	~~Authors: Leonetti, M.D., Yuan, P., MacKinnon, R.~~	+	===Structure of the human SLO3 gating ring===

-	~~Description:~~ Structure ~~of the human SLO3 gating ring~~	+
		+	==About this Structure==
		+	[[4hpf]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HPF OCA].
		+	[[Category: Homo sapiens]]
		+	[[Category: Leonetti, M D.]]
		+	[[Category: MacKinnon, R.]]
		+	[[Category: Yuan, P.]]
		+	[[Category: Membrane protein]]
		+	[[Category: Ph-gated]]
		+	[[Category: Potassium channel]]
		+	[[Category: Transport protein]]

OCA: Protected "4hpf" [edit=sysop:move=sysop]

OCA — Wed, 31 Oct 2012 08:43:25 GMT

Protected "4hpf" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:43, 31 October 2012

OCA: New page: '''Unreleased structure''' The entry 4hpf is ON HOLD Authors: Leonetti, M.D., Yuan, P., MacKinnon, R. Description: Structure of the human SLO3 gating ring

OCA — Wed, 31 Oct 2012 08:43:24 GMT

New page: '''Unreleased structure''' The entry 4hpf is ON HOLD Authors: Leonetti, M.D., Yuan, P., MacKinnon, R. Description: Structure of the human SLO3 gating ring

New page

'''Unreleased structure'''

The entry 4hpf is ON HOLD

Authors: Leonetti, M.D., Yuan, P., MacKinnon, R.

Description: Structure of the human SLO3 gating ring