4hte - Revision history

OCA at 08:12, 9 November 2022

OCA — Wed, 09 Nov 2022 08:12:34 GMT

←Older revision		Revision as of 08:12, 9 November 2022
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	==Crystal Structure of the C-terminal domain of Nicking Enzyme from Staphylococcus aureus==		==Crystal Structure of the C-terminal domain of Nicking Enzyme from Staphylococcus aureus==
-	<StructureSection load='4hte' size='340' side='right' caption='[[4hte]], [[Resolution\|resolution]] 3.00Å' scene=''>	+	<StructureSection load='4hte' size='340' side='right'caption='[[4hte]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4hte]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus~~ aureus~~" (rosenbach 1884) zopf 1885~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HTE OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4HTE FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4hte]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HTE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4HTE FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	~~<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat">~~<scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>~~</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4hte FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hte OCA], [https://pdbe.org/4hte PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4hte RCSB], [https://www.ebi.ac.uk/pdbsum/4hte PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4hte ProSAT]</span></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ht4\|4ht4]]</td></tr>~~	+
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">nes, NES from pLW1043, PGO1_p08, SAP014A_018, SAP015D_002, SAP068A_007, SAP069A_033, SAP079A_020, SAP080A_038, SAP082A_025, VRA0057 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 "Micrococcus aureus" (Rosenbach 1884) Zopf 1885])</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=4hte FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hte OCA], [~~http~~://pdbe.org/4hte PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=4hte RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/4hte PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=4hte ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/O87361_STAAU O87361_STAAU]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Betts, L~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Alpha-helical~~]]	+	[[Category: Staphylococcus aureus]]
-	[[Category: ~~Conjugative transfer]]~~	+	[[Category: Betts L]]
-	~~[[Category: Dna relaxase]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Nes cterminal domain]]~~	+
-	~~[[Category: Vancomycin resistance plasmid~~]]	+

OCA at 07:28, 23 December 2016

OCA — Fri, 23 Dec 2016 07:28:31 GMT

←Older revision		Revision as of 07:28, 23 December 2016
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		+
	==Crystal Structure of the C-terminal domain of Nicking Enzyme from Staphylococcus aureus==		==Crystal Structure of the C-terminal domain of Nicking Enzyme from Staphylococcus aureus==
	<StructureSection load='4hte' size='340' side='right' caption='[[4hte]], [[Resolution\|resolution]] 3.00Å' scene=''>		<StructureSection load='4hte' size='340' side='right' caption='[[4hte]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[4hte]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Staphylococcus_aureus Staphylococcus~~ aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HTE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HTE FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[4hte]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HTE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HTE FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ht4\|4ht4]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ht4\|4ht4]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">nes, NES from pLW1043, PGO1_p08, SAP014A_018, SAP015D_002, SAP068A_007, SAP069A_033, SAP079A_020, SAP080A_038, SAP082A_025, VRA0057 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 ~~Staphylococcus~~ aureus])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">nes, NES from pLW1043, PGO1_p08, SAP014A_018, SAP015D_002, SAP068A_007, SAP069A_033, SAP079A_020, SAP080A_038, SAP082A_025, VRA0057 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 "Micrococcus aureus" (Rosenbach 1884) Zopf 1885])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hte FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hte OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4hte RCSB], [http://www.ebi.ac.uk/pdbsum/4hte PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hte FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hte OCA], [http://pdbe.org/4hte PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4hte RCSB], [http://www.ebi.ac.uk/pdbsum/4hte PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4hte ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 4hte" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Staphylococcus aureus]]
	[[Category: Betts, L]]		[[Category: Betts, L]]
	[[Category: Alpha-helical]]		[[Category: Alpha-helical]]

OCA at 09:47, 10 December 2014

OCA — Wed, 10 Dec 2014 09:47:57 GMT

←Older revision		Revision as of 09:47, 10 December 2014
Line 1:		Line 1:
-	[[~~Image:~~4hte.~~png~~\|~~left~~\|~~200px~~]]	+	==Crystal Structure of the C-terminal domain of Nicking Enzyme from Staphylococcus aureus==
		+	<StructureSection load='4hte' size='340' side='right' caption='[[4hte]], [[Resolution\|resolution]] 3.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[4hte]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HTE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HTE FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[4ht4\|4ht4]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">nes, NES from pLW1043, PGO1_p08, SAP014A_018, SAP015D_002, SAP068A_007, SAP069A_033, SAP079A_020, SAP080A_038, SAP082A_025, VRA0057 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 Staphylococcus aureus])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hte FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hte OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4hte RCSB], [http://www.ebi.ac.uk/pdbsum/4hte PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Multidrug-resistant Staphylococcus aureus infections pose a significant threat to human health. Antibiotic resistance is most commonly propagated by conjugative plasmids like pLW1043, the first vancomycin-resistant S. aureus vector identified in humans. We present the molecular basis for resistance transmission by the nicking enzyme in S. aureus (NES), which is essential for conjugative transfer. NES initiates and terminates the transfer of plasmids that variously confer resistance to a range of drugs, including vancomycin, gentamicin, and mupirocin. The NES N-terminal relaxase-DNA complex crystal structure reveals unique protein-DNA contacts essential in vitro and for conjugation in S. aureus. Using this structural information, we designed a DNA minor groove-targeted polyamide that inhibits NES with low micromolar efficacy. The crystal structure of the 341-residue C-terminal region outlines a unique architecture; in vitro and cell-based studies further establish that it is essential for conjugation and regulates the activity of the N-terminal relaxase. This conclusion is supported by a small-angle X-ray scattering structure of a full-length, 665-residue NES-DNA complex. Together, these data reveal the structural basis for antibiotic multiresistance acquisition by S. aureus and suggest novel strategies for therapeutic intervention.

-	~~{{STRUCTURE_4hte\| PDB=4hte \| SCENE= }}~~	+	Molecular basis of antibiotic multiresistance transfer in Staphylococcus aureus.,Edwards JS, Betts L, Frazier ML, Pollet RM, Kwong SM, Walton WG, Ballentine WK 3rd, Huang JJ, Habibi S, Del Campo M, Meier JL, Dervan PB, Firth N, Redinbo MR Proc Natl Acad Sci U S A. 2013 Jan 28. PMID:23359708<ref>PMID:23359708</ref>

-	~~===Crystal Structure~~ of the ~~C-terminal domain~~ of ~~Nicking Enzyme from Staphylococcus aureus===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_23359708}}~~	+	== References ==
-		+	<references/>
-	==~~About this Structure~~==	+	__TOC__
-	~~[[4hte]] is a 1 chain structure with sequence from [http:~~//~~en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HTE OCA].~~	+	</StructureSection>
	[[Category: Staphylococcus aureus]]		[[Category: Staphylococcus aureus]]
-	[[Category: Betts, L.]]	+	[[Category: Betts, L]]
	[[Category: Alpha-helical]]		[[Category: Alpha-helical]]
	[[Category: Conjugative transfer]]		[[Category: Conjugative transfer]]

OCA at 09:42, 13 February 2013

OCA — Wed, 13 Feb 2013 09:42:05 GMT

←Older revision		Revision as of 09:42, 13 February 2013
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-	[[Image:4hte.~~jpg~~\|left\|200px]]	+	[[Image:4hte.png\|left\|200px]]

	{{STRUCTURE_4hte\| PDB=4hte \| SCENE= }}		{{STRUCTURE_4hte\| PDB=4hte \| SCENE= }}
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	===Crystal Structure of the C-terminal domain of Nicking Enzyme from Staphylococcus aureus===		===Crystal Structure of the C-terminal domain of Nicking Enzyme from Staphylococcus aureus===

		+	{{ABSTRACT_PUBMED_23359708}}

	==About this Structure==		==About this Structure==

OCA at 12:40, 30 January 2013

OCA — Wed, 30 Jan 2013 12:40:43 GMT

←Older revision		Revision as of 12:40, 30 January 2013
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:4hte.jpg\|left\|200px]]

-	~~The entry~~ 4hte ~~is ON HOLD~~ ~~until Paper Publication~~	+	{{STRUCTURE_4hte\| PDB=4hte \| SCENE= }}

-	~~Authors: Betts, L.~~	+	===Crystal Structure of the C-terminal domain of Nicking Enzyme from Staphylococcus aureus===

-	~~Description: Crystal~~ Structure ~~of the C-terminal domain of Nicking Enzyme~~ from Staphylococcus aureus	+
		+	==About this Structure==
		+	[[4hte]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HTE OCA].
		+	[[Category: Staphylococcus aureus]]
		+	[[Category: Betts, L.]]
		+	[[Category: Alpha-helical]]
		+	[[Category: Conjugative transfer]]
		+	[[Category: Dna relaxase]]
		+	[[Category: Hydrolase]]
		+	[[Category: Nes cterminal domain]]
		+	[[Category: Vancomycin resistance plasmid]]

OCA at 14:03, 28 November 2012

OCA — Wed, 28 Nov 2012 14:03:45 GMT

←Older revision		Revision as of 14:03, 28 November 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 4hte is ON HOLD	+	The entry 4hte is ON HOLD until Paper Publication

	Authors: Betts, L.		Authors: Betts, L.

	Description: Crystal Structure of the C-terminal domain of Nicking Enzyme from Staphylococcus aureus		Description: Crystal Structure of the C-terminal domain of Nicking Enzyme from Staphylococcus aureus

OCA at 09:06, 21 November 2012

OCA — Wed, 21 Nov 2012 09:06:52 GMT

←Older revision		Revision as of 09:06, 21 November 2012
Line 3:		Line 3:
	The entry 4hte is ON HOLD		The entry 4hte is ON HOLD

-	Authors: Betts, L	+	Authors: Betts, L.

	Description: Crystal Structure of the C-terminal domain of Nicking Enzyme from Staphylococcus aureus		Description: Crystal Structure of the C-terminal domain of Nicking Enzyme from Staphylococcus aureus

OCA: Protected "4hte" [edit=sysop:move=sysop]

OCA — Thu, 08 Nov 2012 13:27:22 GMT

Protected "4hte" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:27, 8 November 2012

OCA: New page: '''Unreleased structure''' The entry 4hte is ON HOLD Authors: Betts, L Description: Crystal Structure of the C-terminal domain of Nicking Enzyme from Staphylococcus aureus

OCA — Thu, 08 Nov 2012 13:27:21 GMT

New page: '''Unreleased structure''' The entry 4hte is ON HOLD Authors: Betts, L Description: Crystal Structure of the C-terminal domain of Nicking Enzyme from Staphylococcus aureus

New page

'''Unreleased structure'''

The entry 4hte is ON HOLD

Authors: Betts, L

Description: Crystal Structure of the C-terminal domain of Nicking Enzyme from Staphylococcus aureus